Abstract:
:Recent increasing evidence supports a role for neuronal type signaling in bone. Specifically glutamate receptors have been found in cells responsible for bone remodeling, namely the osteoblasts and the osteoclasts. While most studies have focused on ionotropic glutamate receptors, the relevance of the metabotropic glutamate signaling in bone is poorly understood. Specifically type 1 metabotropic glutamate (mGlu1) receptors are expressed in bone, but the effect of its ablation on skeletal development has never been investigated. Here we report that Grm1crv4/crv4 mice, homozygous for an inactivating mutation of the mGlu1 receptor, and mainly characterized by ataxia and renal dysfunction, exhibit decreased body weight, bone length and bone mineral density compared to wild type (WT) animals. Blood analyses of the affected mice demonstrate the absence of changes in circulating factors, such as vitamin D and PTH, suggesting renal damage is not the main culprit of the skeletal phenotype. Cultures of osteoblasts lacking functional mGlu1 receptors exhibit less homogeneous collagen deposition than WT cells, and present increased expression of osteocalcin, a marker of osteoblast maturation. These data suggest that the skeletal damage is directly linked to the absence of the receptor, which in turn leads to osteoblasts dysfunction and earlier maturation. Accordingly, skeletal histomorphology suggests that Grm1crv4/crv4 mice exhibit enhanced bone maturation, resulting in premature fusion of the growth plate and shortened long bones, and further slowdown of bone apposition rate compared to the WT animals. In summary, this work reveals novel functions of mGlu1 receptors in the bone and indicates that in osteoblasts mGlu1 receptors are necessary for production of normal bone matrix, longitudinal bone growth, and normal skeletal development.
journal_name
Bonejournal_title
Boneauthors
Musante I,Mattinzoli D,Otescu LA,Bossi S,Ikehata M,Gentili C,Cangemi G,Gatti C,Emionite L,Messa P,Ravazzolo R,Rastaldi MP,Riccardi D,Puliti Adoi
10.1016/j.bone.2016.10.025subject
Has Abstractpub_date
2017-01-01 00:00:00pages
114-123eissn
8756-3282issn
1873-2763pii
S8756-3282(16)30322-2journal_volume
94pub_type
杂志文章相关文献
BONE文献大全abstract::It is well known that porosity has an inverse relationship with the mechanical properties of bones. We examined cortical and trabecular porosity of antlers, and mineral composition, thickness and mechanical properties in the cortical wall. Samples belonged to two deer populations: a captive population of an experiment...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.10.026
更新日期:2012-01-01 00:00:00
abstract::Osteocytes play an important role in signaling within bone. Communication of osteocytes with each other and with bone lining cells may have a function in mineral homeostasis and mechanotransduction. However, very little is known of the expression of ion channels in these cells. Using the whole-cell patch-clamp techniq...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(00)00439-7
更新日期:2001-01-01 00:00:00
abstract::LMP1 is an intracellular scaffold protein that contains a PDZ domain and three LIM domains. LMP1 has multiple functions including regulating mesenchymal stem cell (MSC) osteogenesis. Gene delivery of LMP1 induces bone formation in vivo in heterotopic and orthotopic sites. However, little is known about the physiologic...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.03.013
更新日期:2010-07-01 00:00:00
abstract::Low body weight is associated with an increased risk for osteoporosis and fractures, but the contribution of other lifestyle related factors have not been previously studied within lean elderly women. The present study evaluated the association between lifelong lifestyle factors and bone density, falls and postmenopau...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.01.143
更新日期:2006-08-01 00:00:00
abstract::Muscle and bone are two intimately connected tissues. A coordinated interplay between these tissues at mechanical levels is required for their development, function and ageing. Evidence is emerging that several genes and molecular pathways exert a pleiotropic effect on both muscle and bone. Bone morphogenetic proteins...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.05.023
更新日期:2015-11-01 00:00:00
abstract:INTRODUCTION:The RANKL-OPG axis is a key regulator of osteoclastogenesis and bone turnover activity. Its contribution to bone resorption under altered mechanical states, however, has not been fully elucidated. Here we examined the role of OPG in regulating mechanically induced bone modeling in a rat model of orthodonti...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.04.194
更新日期:2007-09-01 00:00:00
abstract:BACKGROUND:Good bone and joint health is essential for the physical tasks of daily living and poorer indicators of physical capability in older adults have been associated with increased mortality rates. Genetic variants of indicators of bone and joint health may be associated with measures of physical capability. MET...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.10.004
更新日期:2013-01-01 00:00:00
abstract::One hundred Sprague-Dawley rats were ovariectomized (OVX) or sham-operated (Sham) at the age of 12 weeks. Eight or six animals from each group were sacrificed at the following time points: 0, 2, 4, 8, 16, 28, and 40 weeks. Mechanical testing of the bones showed that the maximal load of the femoral neck remained at the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(96)00369-9
更新日期:1997-03-01 00:00:00
abstract:OBJECTIVE:Autosomal recessive osteopetrosis (ARO) is a rare congenital disorder of defective bone resorption. The inability of osteoclasts to resorb bone compromises the development of bone marrow cavity, and ultimately, leads to defective hematopoiesis and death within the first decade. The only curative treatment cur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115144
更新日期:2020-01-01 00:00:00
abstract::Mandibular condylar cartilage can be distinguished from articular and growth cartilages of long bones based on several differences in morphology, physiology, and function between these structures. However, there is almost no information available on the types of genes that contribute to these differences. In this stud...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.09.048
更新日期:2008-06-01 00:00:00
abstract::Impact microindentation (IMI) is a Reference Point Indentation technique measuring tissue-level properties of cortical bone in humans in vivo. The nature, however, of the properties that can affect bone strength is incompletely understood. In the present study we examined bone material properties in transiliac bone bi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115110
更新日期:2020-02-01 00:00:00
abstract::Parathyroid hormone (PTH) is used clinically in osteoporotic patients to increase bone mass by enhancing bone formation. PTH therapy is not uniformly effective at all skeletal sites and "life-style" factors may modulate the skeletal response to PTH. Alcohol may represent one of these factors. Chronic alcohol abuse is ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.12.221
更新日期:2008-04-01 00:00:00
abstract::The amount of bone turnover in the skeleton has been identified as a predictor of fracture risk independent of areal bone mineral density (aBMD) and is increasingly cited as an explanation for discrepancies between areal bone mineral density and fracture risk. A number of mechanisms have been proposed to explain how b...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2008.02.001
更新日期:2008-06-01 00:00:00
abstract:INTRODUCTION:Mechanical stresses on the bone are an important aspect of physical activity that promotes bone preservation and increases in bone mass. Exercise intensities leading to bone preservation and accrual have not been adequately defined for humans in general, and postmenopausal women in particular. MATERIALS A...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2007.06.009
更新日期:2007-10-01 00:00:00
abstract::We previously reported that a type II sodium phosphate (Na(+)-Pi) cotransporter (Npt2) protein is expressed in osteoclasts and that Pi limitation decreases osteoclast-mediated bone resorption in vitro. We also demonstrated that mice homozygous for the disrupted Npt2 gene (Npt2-/-) exhibit a unique age-dependent bone p...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00601-9
更新日期:2001-11-01 00:00:00
abstract::Paget's disease of bone (PDB) is a chronic bone metabolic disorder. Currently, PDB is the second most frequent bone disorder. PDB is a focal disorder affecting the skeleton segmentally but the cause of which is unknown. It has been hypothesised that somatic mutations could be responsible for the mosaicism described in...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.04.003
更新日期:2018-07-01 00:00:00
abstract::Osteonecrosis of the jaw (ONJ) is now defined by the presence of exposed bone in the mouth, which fails to heal after appropriate intervention over a period of six or eight weeks. It is commonly precipitated by a tooth extraction in patients treated with zoledronate, pamidronate or a combination of these agents, for t...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2008.09.012
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The treatment of complex bone loss scenarios remains challenging. This study evaluates the efficacy of ex vivo regional gene therapy using transduced human adipose-derived stem cells (ASCs) overexpressing bone morphogenetic protein-2 (BMP-2) to treat critical-sized bone defects. METHODS:Critical-sized femor...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115524
更新日期:2020-09-01 00:00:00
abstract::The Mediterranean osteoporosis (MEDOS) study was carried out in 14 centres from six countries in Southern Europe to determine the incidence rates and risk factors associated with hip fracture over the age of 50 years. This paper discusses both the validity and relevance of the data, that is, whether the number of coll...
journal_title:Bone
pub_type: 杂志文章,多中心研究
doi:10.1016/8756-3282(93)90349-f
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Roux-en-Y gastric bypass (RYGB) is a common surgical procedure for treatment of morbid obesity. RYGB induces considerable and sustained weight loss, and remission of obesity related-comorbidities. While studies have suggested negative effects of RYGB on bone health, long-term data are lacking. We aimed to ev...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.07.014
更新日期:2019-10-01 00:00:00
abstract::FGF23 is an O-glycosylated circulating peptide hormone with a critical role in phosphate homeostasis; it is inactivated by cellular proprotein convertases in a pre-release degradative pathway. We have here examined the metabolism of FGF23 in a model bone cell line, IDG-SW3, prior to and following differentiation, as w...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.12.055
更新日期:2016-03-01 00:00:00
abstract::Sulfate conjugation is an important pathway in the metabolism of many drugs, xenobiotic compounds, and hormones. Sulfotransferases (SULTs) catalyze these reactions and have been detected and characterized in various human tissues including the liver and small intestine. Substrates for SULTs that include estrogen and t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00463-x
更新日期:2001-06-01 00:00:00
abstract::The effects of prostaglandin E2 (PGE2) were histomorphometrically evaluated in cancellous bone of the axial skeleton of ovariectomized, osteopenic rats. Four months following bilateral ovariectomy (OVX) and sham-ovariectomy (SHAM) at 3 months of age, rats received daily subcutaneous injections of PGE2 at 0, 0.3, 1.0, ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(90)90057-6
更新日期:1990-01-01 00:00:00
abstract::Bone structure is an important determinant of osteoporotic fracture. In women, bone structure is highly heritable, and several quantitative trait loci (QTL) have been reported. There are few comparable data in men. This study in men aimed at establishing the heritability of bone structure at the proximal femur, identi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.05.005
更新日期:2005-10-01 00:00:00
abstract::Bone is a nanocomposite consisting of two main components, nano-hydroxyapatite (n-HA) and Type I collagen (Col). The aim is to exploit the nano-scale functional and material characteristics of natural bone in order to modulate cellular functions for optimal bone repair in bone graft systems. Here, we present an effect...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.03.674
更新日期:2009-07-01 00:00:00
abstract::Osteoporosis is a major cause of disability in the United States. Numerous factors contribute to the decline in bone mineral density (BMD) that characterizes this disease, and the importance of heredity is now widely appreciated. We evaluated the joint contributions of genes and environmental factors on variation in B...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00246-1
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:Vertebral fractures (VF) upon Denosumab (DMAB) discontinuation were first described as a distinct phenomenon in 2015, yet the magnitude of this event remains undetermined. OBJECTIVES:To estimate fracture risk after DMAB discontinuation, in a real-world setting. METHODS:The computerized database of a 2.3-mi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115150
更新日期:2020-01-01 00:00:00
abstract::The present work was designed to investigate the effects of oscillating fluid flow on gap junctional intercellular communication (GJIC) and the gap junction protein connexin (Cx) 43 in osteocyte-like MLOY-4 cells. Cells were exposed for 1 h to oscillating fluid flow at a shear stress of +/-10 dyn/cm(2) and a frequency...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00167-4
更新日期:2003-07-01 00:00:00
abstract::A theory is developed to resolve several inconsistencies between current concepts and observations about bone remodeling. For example, the observation that remodeling increases both when mechanical loading is excessively low, that is, in a disuse state, and when it is excessively high, producing substantial fatigue da...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00241-0
更新日期:2000-01-01 00:00:00
abstract::The study reports a theranostic nature of rno-miR-300 (miR300) in the osteoblast functioning, by influencing the signaling pathway(s), associated with osteoblast differentiation. Excessive expression of miR300 suppresses osteoblast functions. Smad3 served as a validated target for miR300, on homology-based computation...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115603
更新日期:2021-02-01 00:00:00