Abstract：
:Infection by SARS-CoV-2 commonly begins in the nasopharynx, and the cytologic and molecular correlates are not characterized. Fifty-eight cytologic preps (20 oral and 38 from the nasopharynx) were obtained from ten patients and analyzed in a blinded fashion for SARS-CoV-2 spike and envelope protein by immunohistochemistry and viral RNA by in situ hybridization. qRTPCR identified three positive cases and seven controls; the three cases reported mild symptoms that resolved in 2-3 days. Blinded analyses confirmed the presence of the SARS-CoV-2 spike and envelope proteins and viral RNA in the three cases and viral absence in the seven controls. A signal for the positive cases was evident in each nasopharyngeal and none of the oral samples. Viral RNA/proteins localized exclusively to glandular cells and was present in high copy number. Blinded analysis of the cytology documented that the glandular cells infected by SARS-CoV-2 showed marked degeneration with ciliocytophthoria; viral inclusions were not evident. Co-expression analysis showed viral infected cells had increased apoptosis, marked by strong expression of activated caspase 3. Weekly serial testing of two of the cases showed persistence of productive viral infection for up to 2 weeks after symptom onset. It is concluded that the target cell of SARS-CoV-2 in the head and neck region is the glandular cell of the nasal passages, that viral infection is lytic and associated with high copy number that facilitates viral spread. The method outlines a simple, rapid test for productive SARS-CoV-2 based on immunohistochemistry or in situ hybridization of the glandular cells from the nasopharynx.
journal_name
Ann Diagn Patholjournal_title
Annals of diagnostic pathologyauthors
Nuovo GJ,Magro C,Mikhail Adoi
10.1016/j.anndiagpath.2020.151565subject
Has Abstractpub_date
2020-10-01 00:00:00pages
151565eissn
1092-9134issn
1532-8198pii
S1092-9134(20)30108-8journal_volume
48pub_type
杂志文章abstract：:Myofibrosarcomas of the tibia are exceedingly rare, with only one case reported in the literature. We describe DNA ploidy of high-grade myofibrosarcoma of the tibia in correlation with clinicomorphologic and ultrastructural features in a 16-year-old adolescent girl. Radiological studies revealed an expanding osteolyti...
journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
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journal_title：Annals of diagnostic pathology
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2007.01.012
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
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journal_title：Annals of diagnostic pathology
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2009.06.012
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2010.05.002
更新日期：2010-08-01 00:00:00
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2007.08.001
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2014.03.008
更新日期：2014-08-01 00:00:00
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2012.08.004
更新日期：2013-08-01 00:00:00
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2011.04.006
更新日期：2011-10-01 00:00:00
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/s1092-9134(99)80029-x
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2020.151593
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章,评审
doi：10.1016/s1092-9134(99)80006-9
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2016.05.002
更新日期：2016-08-01 00:00:00
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2015.02.007
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2016.02.001
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2011.04.010
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journal_title：Annals of diagnostic pathology
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doi：10.1016/j.anndiagpath.2007.03.001
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journal_title：Annals of diagnostic pathology
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journal_title：Annals of diagnostic pathology
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doi：10.1016/j.anndiagpath.2013.03.003
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journal_title：Annals of diagnostic pathology
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doi：10.1016/j.anndiagpath.2009.05.006
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章,评审
doi：10.1016/j.anndiagpath.2006.02.003
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2006.06.008
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2010.03.003
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章
doi：10.1016/j.anndiagpath.2007.10.001
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journal_title：Annals of diagnostic pathology
pub_type： 杂志文章,评审
doi：10.1016/j.anndiagpath.2011.10.001
更新日期：2012-06-01 00:00:00
abstract：:In the recent years, basaloid squamous cell carcinomas (BSCCs) have gained attention because of (1) observation of a relative increase in the number of tumors arising particularly in head and neck sites, (2) identification of human papillomavirus (HPV) in BSCCs arising predominantly in the oropharynx, and (3) controve...
journal_title：Annals of diagnostic pathology
pub_type： 杂志文章,评审
doi：10.1016/j.anndiagpath.2013.09.001
更新日期：2013-12-01 00:00:00
