Abstract：
:Osteosarcoma is characterized by high malignancy and high metastasis rate, resulting in high mortality and disability. MiR-663a has been reported in a variety of tumors to promote tumorigenesis. However, miR-663a has not been reported in the pathogenesis of osteosarcoma. Bioinformatics analysis and experiments including real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Western blot, RNA immunoprecipitation, and flow cytometry assay were applied to explore the function and mechanism of miR-663a in MG63, U2OS, Saos-2, SF-86, and hFOB1.19 cells. In this study, we found that miR-663a is highly expressed in osteosarcoma. At the same time, we discovered that miR-663a facilitates cell proliferation and migration, whereas suppresses cell apoptosis in osteosarcoma. Through a series of biological experiments, it was found that miR-663a regulates the cellular process in osteosarcoma by modulating the expression of MYL9. In addition, we also found that long noncoding RNA (lncRNA) GAS5 serves as a molecular sponge for miR-663a and regulates the progression of osteosarcoma via the ceRNA mechanism. We uncover that miR-663a promotes osteosarcoma development through targeting MYL9, which was regulated by lncRNA GAS5.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Zhao S,Xiong W,Xu Kdoi
10.1177/0960327120937330subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1607-1618issue
12eissn
0960-3271issn
1477-0903journal_volume
39pub_type
杂志文章abstract：:Seafood is considered by nutritionists to be a high source of omega 3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs) which are involved in prevention of cardiovascular diseases. The aim of the present study is firstly to detect the presence of organochlorine pesticides and heavy metals in some fresh seafood of ...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327110396535
更新日期：2011-10-01 00:00:00
abstract：:The aim of the present investigation was to check the fluctuation in essential elements, such as Na, K, Mg, Mn, Cu, Zn, Cr and Ni in the brain, spinal cord, liver and kidney of mice during methylmercury chloride (MMC) toxication and therapy with monothiols (N-acetyl-DL-homocysteine thiolactone and glutathione) and vit...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719401301201
更新日期：1994-12-01 00:00:00
abstract：:Naringenin is a naturally occurring flavanones and has been found to exhibit free radical scavenging, enzyme inhibition, antioxidants, anti-inflammatory, and anticancer activities. Present study was designed to evaluate the protective role of naringenin against benzo[a]pyrene (B[a]P)-induced oxidative stress and pulmo...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327116650009
更新日期：2017-04-01 00:00:00
abstract：:Organic solvents can cause hearing loss themselves or promote noise-induced hearing loss. The objective of this study was to review the literature on the effects of low-level exposure to trichloroethylene on the auditory system and consider its relevance for the occupational settings. Both human and animal investigati...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327108090267
更新日期：2008-03-01 00:00:00
abstract：:Sodium hypochlorite (NaClO) is used extensively as a disinfectant or bleaching agent. Most studies describe ingestion or inhalation route of this product with rare complication and fatalities. Despite global daily exposures, data about bleach injection is limited. Here we report intentional infusion of 20 mL, sodium h...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327111432506
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:The objective of the present study was to determine oxidant and antioxidant status in infants with hyperbilirubinemia and/or kernicterus and to find whether there is a relationship between bilirubin level and oxidant/antioxidant status. PATIENTS:The study includes 69 full-term newborns (neonates with hyperbi...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327111401638
更新日期：2011-11-01 00:00:00
abstract：:In vivo antidotal efficacy of new bis- quaternary 2-(hydroxyimino)- N-(pyridin-3yl) acetamide derivatives (HNK series), to counter multiples of lethal doses of nerve agent sarin (GB) and reactivation of acetylcholinesterase (AChE), was evaluated in Swiss albino mice. [Protection index PI; median lethal dose (LD50) of ...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327116637109
更新日期：2017-01-01 00:00:00
abstract：:Cultures of bovine pulmonary endothelial (BPE) cells were exposed to LC70 doses of ricin or abrin (15.5 and 4.5 pM respectively) over a period of up to 40 h. The viability of the cultures (as determined by the neutral red (NR) dye retention assay) declined after 6 h exposure to the toxins. From 15 h onwards, cellular ...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719601500513
更新日期：1996-05-01 00:00:00
abstract：:The study was done to find out whether subacute exposure to supercypermethrin forte (SCM) affected cellular and humoral immunity. Groups of 10 male Wistar rats were given SCM by gavage for 28 days at 12.5 mg kg-1 day-1 (1/14 LD50), 8.75 mg kg-1 day-1 (1/20 LD50) and 4.38 mg kg-1 day-1 (1/40 LD50) and the response of s...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719501400502
更新日期：1995-05-01 00:00:00
abstract：:1. Three indexes for evaluating the severity of paraquat poisoning based on plasma-paraquat concentration are presently in use; the curves of Proudfood et al. and Scherrmann et al. and the SIPP. Their effectiveness in determining the prognosis of patients with paraquat poisoning was evaluated. 2. To determine which in...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719101000104
更新日期：1991-01-01 00:00:00
abstract：:The majority of households in rural India still rely on unprocessed solid biomass for domestic energy. The aim of this study was to investigate whether chronic exposure to biomass smoke causes activation of leukocytes and the formation of leukocyte-platelet aggregates. We conducted flow cytometric analysis of beta2 Ma...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327106074603
更新日期：2006-11-01 00:00:00
abstract：:Personal reflections on radiation hormesis for the past 50 years are presented. The causes of ignoring and rejections of this phenomenon by international and national bodies and by radiation protection establishment are analyzed. The opposition against nuclear weapons and preparations for nuclear war was probably the ...
journal_title：Human & experimental toxicology
pub_type： 历史文章,杂志文章,评审
doi：10.1177/0960327110363974
更新日期：2010-04-01 00:00:00
abstract：:Sibutramine mesylate is a new anti-obesity drug. It is a crystalline salt of sibutramine developed to improve the solubility of sibutramine hydrochloride. Methanesulfonic acid was used as a salt-forming acid instead of hydrochloric acid, resulting in a greatly improved solubility of 1000 mg/mL in water. Sibutramine me...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1191/0960327105ht510oa
更新日期：2005-03-01 00:00:00
abstract：:Hydrogen sulfide (H2S) is an endogenously produced gaseous messenger that participates in regulation of cardiovascular functions. This study evaluates the possible protective effect of H2S in cardiovascular dysfunction induced by cecal ligation and puncture (CLP) in rats. After 24 h of induction of CLP, heart rate (HR...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327114564794
更新日期：2015-10-01 00:00:00
abstract：:Fructus Psoraleae (FP), widely used in traditional medicine, is increasingly reported to cause serious hepatotoxicity in recent years. However, the main toxic constituents responsible for hepatotoxicity and the underlying mechanisms are poorly understood. In the present study, psoralen, a main and quality-control cons...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327120979346
更新日期：2020-12-15 00:00:00
abstract：:1. Multiple low doses of the direct acting organophosphates, ecothiopate, paraoxon and mipafox produced persistent and additive inhibition of diaphragm acetylcholinesterase. Paraoxon and mipafox had similar effects on brain acetylcholinesterase. There was greater recovery from inhibition between doses for paraoxon and...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719701600201
更新日期：1997-02-01 00:00:00
abstract：:This study deals with pesticide exposure profile in some European countries with a specific focus on ethylenebisdithiocarbamates (EBDC). In all, 55 Bulgarian greenhouse workers, 51 Finnish potato farmers, 48 Italian vineyard workers, 42 Dutch floriculture farmers, and 52 Bulgarian zineb producers entered the study. Ea...
journal_title：Human & experimental toxicology
pub_type： 杂志文章,多中心研究
doi：10.1177/0960327108100003
更新日期：2008-09-01 00:00:00
abstract：:The time-dependent changes in lead (Pb) concentrations in major tissues, serum and urine, and the Pb biomarker delta-aminolevulinic acid (ALA) concentration in urine were studied in rats after sub-chronic Pb exposure. Female Sprague-Dawley (SD) rats were exposed to Pb in drinking water at concentrations of 100 ppm and...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327109107046
更新日期：2009-10-01 00:00:00
abstract：:Brain tumors are often incurable despite current aggressive treatment modalities. Regional intracerebral administration of labeled monoclonal antibodies (Mabs) can maximize the radioisotope and Mab concentration to tumor sites while reducing systemic toxicity. h-R3 is a humanized antiepidermal growth factor receptor M...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1191/096032700675323269
更新日期：2000-12-01 00:00:00
abstract：:Prolonged zidovudine (AZT) treatment in HIV-infected and AIDS patients is shown to induce liver toxicity leading to complications. Therapeutic regimen that could encounter this adverse effect is unavailable and management of toxicity is often symptomatic or is limited to withdrawal of therapy. In the present investiga...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327114567765
更新日期：2015-11-01 00:00:00
abstract：:Two previously healthy women developed nausea, vomiting, headache and dizziness for several days, a massive hair loss about 2 weeks later and a discoloration of the fingernails. Detailed diagnostic procedures did not reveal any pathological results. Therapeutic measures did not show any effect. Thallium and arsenic we...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327109360046
更新日期：2010-05-01 00:00:00
abstract：:1. Six healthy male volunteers received intravenous injections of 26Al as citrate. Accelerator mass spectrometry and gamma-ray spectrometry were used to determine levels of the tracer in blood and excreta at times up to 5-6 d. 2. There was a rapid clearance from blood (mean 2% of injection remaining after 1 d) and maj...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719501400707
更新日期：1995-07-01 00:00:00
abstract：:Pesticides are used in agriculture to protect crops from insects-pests. Most of the field workers of North Indian population are exposed to commonly used insecticides. In the present study, pesticides induced oxidative stress as well as alterations in the level of acetylcholinesterase (AChE) in a total of 70 male heal...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327112474835
更新日期：2013-11-01 00:00:00
abstract：:Sibutramine is an antiobesity drug that inhibits the reuptake of serotonin and noradrenalin in the hypothalamus. A 37-year-old Korean man presented to the emergency room for the oral intake of 280 mg of sibutramine. The patient was in thyrotoxic state. The (99m)Technetium-pertechnetate thyroid scan showed irregular up...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327112468907
更新日期：2013-08-01 00:00:00
abstract：:Persistent organochlorine pollutants (POPs), such as polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), dichloro diphenyl trichloroethane (DDT) and its major metabolite 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p' -DDE) have been associated with type 2 diabetes mellitus (T2DM) in rece...
journal_title：Human & experimental toxicology
pub_type： 杂志文章,多中心研究
doi：10.1177/0960327107076886
更新日期：2007-05-01 00:00:00
abstract：:In this study, the effect of a combination of vitamin C, vitamin E and selenium on ethanol-induced duodenal mucosal damage in rats was investigated morphologically and biochemically. The duodenal mucosal injury was produced by oral administration of 1 mL of absolute ethanol to each rat. Animals received vitamin C (250...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1191/0960327104ht468oa
更新日期：2004-08-01 00:00:00
abstract：:Enterococcus faecalis is a facultative anaerobic gram-positive commensal bacterium common in the gastrointestinal tract of animals and humans. This study aimed to investigate the protective effects of heat-killed E. faecalis EF-2001 (EF-2001) on acute gastric ulcer using a murine model of ethanol (EtOH)-induced acute ...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/0960327119899987
更新日期：2020-05-01 00:00:00
abstract：:1. The reactivating and therapeutic efficacy of a new acetylcholinesterase reactivator, designated BI-6 (1-/2-hydroxyiminomethylpyridinium/-4-/carbamoylpyridinium/- 2-butene dibromide), against organophosphate sarin was compared with presently used oximes (pralidoxime, obidoxime, methoxime) and H oximes (HI-6, HLö-7) ...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1191/096032799678845106
更新日期：1999-09-01 00:00:00
abstract：:The pharmacokinetics of the herbicide fluazifop-butyl have been determined in female rats following oral and intravenous dosing, and described by a mathematical model. Penetration of fluazifop-butyl through epidermal membranes has been determined using three different receptor fluids. It is demonstrated how this in vi...
journal_title：Human & experimental toxicology
pub_type： 杂志文章
doi：10.1177/096032719301200302
更新日期：1993-05-01 00:00:00
abstract：:It has been argued that the phenomenon of hormesis should prompt us to revise current regulatory policy in order to take beneficial effects of small doses of various agents into account. I argue that three problems--the comparative smallness of hormetic effects, the fine-tuning problem, and the problem of aggregated a...
journal_title：Human & experimental toxicology
pub_type： 杂志文章,评审
doi：10.1177/0960327108098490
更新日期：2008-08-01 00:00:00
