Abstract:
:Primary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4(+)Foxp3(+) regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Wang D,Zhang H,Liang J,Gu Z,Ma X,Huang J,Lin J,Hou Y,Lu L,Sun Ldoi
10.1007/s10238-010-0105-6subject
Has Abstractpub_date
2011-03-01 00:00:00pages
25-32issue
1eissn
1591-8890issn
1591-9528journal_volume
11pub_type
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doi:10.1007/s10238-010-0128-z
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doi:10.1007/s10238-007-0120-4
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journal_title:Clinical and experimental medicine
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更新日期:2009-06-01 00:00:00
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