Abstract:
:The global emergence and spread of malaria parasites resistant to antimalarial drugs is the major problem in malaria control. The genetic basis of the parasite's resistance to the antimalarial drug chloroquine (CQ) is well-documented, allowing for the analysis of field isolates of malaria parasites to address evolutionary questions concerning the origin and spread of CQ-resistance. Here, we present DNA sequence analyses of both the second exon of the Plasmodium falciparum CQ-resistance transporter (pfcrt) gene and the 5' end of the P. falciparum multidrug-resistance 1 (pfmdr-1) gene in 40 P. falciparum field isolates collected from eight different localities of Odisha, India. First, we genotyped the samples for the pfcrt K76T and pfmdr-1 N86Y mutations in these two genes, which are the mutations primarily implicated in CQ-resistance. We further analyzed amino acid changes in codons 72-76 of the pfcrt haplotypes. Interestingly, both the K76T and N86Y mutations were found to co-exist in 32 out of the total 40 isolates, which were of either the CVIET or SVMNT haplotype, while the remaining eight isolates were of the CVMNK haplotype. In total, eight nonsynonymous single nucleotide polymorphisms (SNPs) were observed, six in the pfcrt gene and two in the pfmdr-1 gene. One poorly studied SNP in the pfcrt gene (A97T) was found at a high frequency in many P. falciparum samples. Using population genetics to analyze these two gene fragments, we revealed comparatively higher nucleotide diversity in the pfcrt gene than in the pfmdr-1 gene. Furthermore, linkage disequilibrium was found to be tight between closely spaced SNPs of the pfcrt gene. Finally, both the pfcrt and the pfmdr-1 genes were found to evolve under the standard neutral model of molecular evolution.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Sutar SK,Gupta B,Ranjit M,Kar SK,Das Adoi
10.1590/s0074-02762011000100013subject
Has Abstractpub_date
2011-02-01 00:00:00pages
78-84issue
1eissn
0074-0276issn
1678-8060pii
S0074-02762011000100013journal_volume
106pub_type
杂志文章abstract::The enzyme-linked immunosorbent assay (Elisa) was used to examine sera of 104 children and adults in Jos, Plateau State, Nigeria for anti-toxocaral antibodies, out of which 31 (29.8%) were reactive. The seropositive rates were 30.4% for adults, 29.6% for children, 34% for females and 25.9% for males. However, the diff...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/S0074-02762000000200002
更新日期:2000-03-01 00:00:00
abstract::The diagnosis of tick-borne diseases such as babesiosis still depends on observing the parasite in the infected erythrocyte. Microscopic observation is tedious and often problematic in both early and carrier infections. Better diagnostic methods are needed to prevent clinical disease, especially when susceptible cattl...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761992000700032
更新日期:1992-01-01 00:00:00
abstract::In this paper a discussion is made on the pathogenesis of schistosomiasis mansoni in mice, presented from the perspectives of "processes", "mediators", "strategies for study" and "disease". These concepts overlap considerably. Regarding "processes", granulomas, fibrosis and vasculitis are discussed. The role of mediat...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761992000800054
更新日期:1992-01-01 00:00:00
abstract::A simple and rapid method for differentiating the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red coloration of the hemoglobin fraction permits visualization of the migration patterns withou...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761986000300008
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:Since the early 1990s, programs to control Chagas disease in South America have focused on eradicating domiciliary Triatoma infestans, the main vector. Seroprevalence studies of the chagasic infection are included as part of the vector control programs; they are essential to assess the impact of vector contr...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760160407
更新日期:2017-05-01 00:00:00
abstract::Genomic DNA fragments from males of Psychodopygus wellcomei were isolated and shown to be useful as sensitive diagnostic probes for positively separating individuals of this species from those of Ps. complexus. These two members of the Ps. squamiventris series are found sympatrically in foci of cutaneous leishmaniasis...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000100008
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. OBJECTIVE:The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760170117
更新日期:2017-12-01 00:00:00
abstract::Preparations of simian rotavirus SA11 maintained in different laboratories were compared with each other by polyacrylamide gel electrophoresis of genomic RNA. Differences in the migration of genome segments 4, 5 and 7 allowed the classification of eight virus preparations into four electrophoretic types. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761986000400005
更新日期:1986-10-01 00:00:00
abstract::Gryllophila cephalobulata n. sp. (Nematoda, Thelastomatidae) a parasite of the mole cricket Neocurtilla claraziana (Orthoptera, Gryllotalpidae) isolated in Buenos Aires Province, is described and illustrated. It is characterized by cuticle annulated all along the length of the body; the first ring has 4 lobules, the s...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762002000500012
更新日期:2002-07-01 00:00:00
abstract::Visceral leishmaniasis, or kala-azar, is recognised as a serious emerging public health problem in India. In this study, environmental parameters, such as land surface temperature (LST) and renormalised difference vegetation indices (RDVI), were used to delineate the association between environmental variables and Phl...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276108022013012
更新日期:2013-04-01 00:00:00
abstract::The diagnosis of meningitic angiostrongyliasis (MA) is based on clinical criteria. A lumbar puncture is used as a diagnostic tool, but it is an invasive procedure. The blood eosinophil levels are also assessed and used in the diagnosis of this disease. We enrolled 47 patients with serologically proven MA and 131 contr...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000700020
更新日期:2010-11-01 00:00:00
abstract::Mycobacterium fortuitum is a rapidly growing nontuberculous Mycobacterium that can cause a range of diseases in humans. Complications from M. fortuitum infection have been associated with numerous surgical procedures. A protective immune response against pathogenic mycobacterial infections is dependent on the granulom...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000500007
更新日期:2010-08-01 00:00:00
abstract::CD8+ T cells have been implicated as critical effector cells in protection against the pre-erythrocytic stage of malaria in mice and humans following irradiated sporozoite immunization. Immunization experiments in animal models by several investigators have suggested different strategies for vaccination against malari...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02762001000200014
更新日期:2001-02-01 00:00:00
abstract::The transference of the genetic markers and the presence of DNA plasmidial in 240 cultures of Escherichia coli was investigated. The strains were originated from Waste Treatment Plant (inffluent and effluents) located in Ilha do Governador, Rio de Janeiro. By conjugation analysis, E. coli K 12 allowed the isolation of...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761988000100004
更新日期:1988-01-01 00:00:00
abstract::Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic m...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000500017
更新日期:2010-08-01 00:00:00
abstract::Granuloma size is the variable most frequently used to evaluate the immunopathogenesis of schistosome infections. However, hepatic fibrosis is at the least an equally relevant variable. Hepatic fibrosis and the size of circumoval granulomas are frequently dissociated in experimental murine Schistosoma mansoni and S. j...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761997000500024
更新日期:1997-09-01 00:00:00
abstract::In this transversal study, factors related to infection with and transmission of Schistosoma mansoni were explored. Based on stool examinations of two Kato-Katz smears of a single sample, the prevalences of schistosomiasis and geohelminths were established. In a multivariable analysis, sets of demographic, socio-econo...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000400037
更新日期:2010-07-01 00:00:00
abstract::Infusions of Aspidosperma nitidum (Apocynaceae) wood bark are used to treat fever and malaria in the Amazon Region. Several species of this family are known to possess indole alkaloids and other classes of secondary metabolites, whereas terpenoids, an inositol and the indole alkaloids harmane-3 acid and braznitidumine...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276130246
更新日期:2013-12-01 00:00:00
abstract::Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761998000600023
更新日期:1998-11-01 00:00:00
abstract::This paper presents the morphological, histological and ultrastructural characteristics of Myxobolus oliveirai sp. nov., a parasite of the gill filaments in Brycon hilarii from the Brazilian Pantanal. Out of 216 B. hilariispecimens examined (126 wild and 90 cultivated), 38.1% of wild specimens (n = 48) were infected. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000600006
更新日期:2010-09-01 00:00:00
abstract::Acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. The significance of acoustic signals for a blood-sucking insect was first reported in the XIX century by Christopher Johnston, studying the hear...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/0074-0276130390
更新日期:2013-01-01 00:00:00
abstract::Analgesic and anti-inflammatory activities of water (WE) and ethanolic (EE) extracts of Scoparia dulcis L. were investigated in rats and mice, and compared to the effects induced by Glutinol, a triterpene isolated by purification of EE. Oral administration (p.o.) of either WE or EE (up to 2 g/kg) did not alter the nor...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000600034
更新日期:1991-01-01 00:00:00
abstract::A study of crepuscular and night-biting mosquitoes was conducted at remote settlements along the Padauiri River, middle Negro River, state of Amazonas, Brazil. Collections were performed with human bait and a CDC-light trap on three consecutive days per month from June 2003-May 2004. In total, 1,203 h of collection we...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762009000100002
更新日期:2009-02-01 00:00:00
abstract::BACKGROUND Studies on malaria vectors in the Pantanal biome, Central Brazil, were conducted more than half a century ago. OBJECTIVES To update anopheline records and assess receptivity and vulnerability to malaria transmission. METHODS Five-day anopheline collections were conducted bimonthly in Salobra, Mato Grosso do...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760170175
更新日期:2018-02-01 00:00:00
abstract::A survey of the carrion fauna was made at two sites in Curitiba, State of Paraná, with the objective of describing the insects associated with carrion and setting up a preliminary data-base for medico-legal purposes in south Brazil. Vertebrate exclusion experiments were carried out in each season between 1994 and 1995...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761997000200023
更新日期:1997-03-01 00:00:00
abstract::A closed Lutzomyia longipalpis colony, from Ceará has been used to transmit Leishmania chagasi isolated from a fox in Pará state. The last time this colony was successfully used in similar transmission experiments was eight years (64 generations) ago indicating that this colony of Lu. longipalpis has fully maintained ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761985000300010
更新日期:1985-07-01 00:00:00
abstract::The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequenc...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761996000100020
更新日期:1996-01-01 00:00:00
abstract::An experimental murine model was used to verify the viability and pathogenicity of coccoid Helicobacter pylori. For this purpose, 27 BALB/c mice were inoculated intragastrically with 1 ml broth culture (10(8)organisms/ml) of a coccoid H. pylori clinical isolate. The animals were divided into two groups. Nine were infe...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762002000800023
更新日期:2002-12-01 00:00:00
abstract::An experimental model for acquired and congenital ocular toxoplasmosis as well as a model to induce experimental autoimmune uveitis (EAU) was investigated in Calomys callosus. Toxoplasma gondii, ME-49 strain, was used to infect males and pregnant- and not pregnant-females while S-antigen, a major glycoprotein of the r...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761999000100021
更新日期:1999-01-01 00:00:00
abstract::Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positi...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761985000300005
更新日期:1985-07-01 00:00:00