CRISPR system: Discovery, development and off-target detection.

abstract：PURPOSE OF REVIEW:microRNAs (miRNAs) are a class of small, evolutionarily conserved, non-coding RNAs (ncRNAs) that function as inhibitors of post-transcriptional mRNA expression. They are implicated in the pathogenesis of numerous diseases, including many common kidney conditions. In this review, we focus on how miRNAs...

journal_title：Cellular signalling

authors： Ramalingam H,Yheskel M,Patel V

更新日期：2020-07-01 00:00:00

abstract：:ASB proteins are the specificity subunits of cullin5-RING E3 ubiquitin ligases (CRL5) that play roles in ubiquitin-mediated protein degradation. However, how their activity is regulated remains poorly understood. Here, we unravel a novel mechanism of regulation of a CRL5 through phosphorylation of its specificity subu...

journal_title：Cellular signalling

authors： Zakaria R,Lamsoul I,Uttenweiler-Joseph S,Erard M,Monsarrat B,Burlet-Schiltz O,Moog-Lutz C,Lutz PG

更新日期：2013-12-01 00:00:00

abstract：:Prostatic stromal proliferation may be commonly associated with the development of benign prostatic hyperplasia. In this study, we investigate the role of testosterone and protein kinase C in stimulating cultured stromal cell proliferation. Testosterone increased the uptake of [(3)H]-thymidine into the human cultured ...

journal_title：Cellular signalling

authors： Haynes JM,Frydenberg M,Majewski H

更新日期：2001-10-01 00:00:00

abstract：:Src homology (SH) 3 domains are small modules found in a diverse array of proteins. The presence of an SH3 domain confers upon its resident protein the ability to interact with specific proline-rich sequences in protein binding partners. A major focus of research has highlighted a role for SH3 domain-mediated interact...

journal_title：Cellular signalling

authors： McPherson PS

更新日期：1999-04-01 00:00:00

abstract：:Calcineurin (CnA) is an important signalling molecule in skeletal muscle, in the promotion of differentiation, slow-fibre phenotype and possibly fibre hypertrophy. We found that stable expression of constitutively active CnA in muscle C2C12 cells strongly activated NF-kappaB, a key mediator of muscle wasting. NF-kappa...

journal_title：Cellular signalling

authors： Alzuherri H,Chang KC

更新日期：2003-05-01 00:00:00

abstract：:Nore1A was originally identified as a potential Ras effector, and Nore1B is an alternatively spliced isoform. Both share a Ras/Rap association domain (RA domain) but only Nore1A contains sequence motifs that predict SH3 domain binding and diacylglycerol/phorbol ester binding in the amino-terminal region. Here we repor...

journal_title：Cellular signalling

authors： Ishiguro K,Avruch J,Landry A,Qin S,Ando T,Goto H,Xavier R

更新日期：2006-10-01 00:00:00

abstract：:Receptor for Activated C-Kinase 1 (RACK1) belongs to the WD40 family of proteins, known to act as scaffolding proteins in interaction networks. Accordingly, RACK1 is found to have numerous interacting partners ranging from kinases and signaling proteins to membrane bound receptors and ion channels. Interestingly, RACK...

journal_title：Cellular signalling

authors： Nielsen MH,Flygaard RK,Jenner LB

更新日期：2017-07-01 00:00:00

abstract：:In humans, thromboxane (TX) A₂ signals through the TPα and TPβ isoforms of its G-protein coupled TXA₂ receptor (TP) to mediate a host of (patho)physiologic responses. Herein, angio-associated migratory cell protein (AAMP) was identified as a novel interacting partner of both TPα and TPβ through an interaction dependen...

journal_title：Cellular signalling

authors： Reid HM,Wikström K,Kavanagh DJ,Mulvaney EP,Kinsella BT

更新日期：2011-04-01 00:00:00

abstract：:Human neutrophils pre-incubated with granulocyte-macrophage-colony-stimulating factor (GM-CSF) exhibit an enhanced mobilization of calcium in response to secondary stimuli such as chemotactic factors. The mechanisms underlying this priming effect of GM-CSF were examined. It was first demonstrated that the additional c...

journal_title：Cellular signalling

authors： Naccache PH,Hamelin B,Gaudry M,Bourgoin S

更新日期：1991-01-01 00:00:00

abstract：:Non-small cell lung cancer is one of the most aggressive cancers as per as the mortality and occurrence is concerned. Paclitaxel based chemotherapeutic regimes are now used as an important option for the treatment of lung cancer. However, resistance of lung cancer cells to paclitaxel continues to be a major clinical p...

journal_title：Cellular signalling

authors： Chatterjee A,Chattopadhyay D,Chakrabarti G

更新日期：2015-02-01 00:00:00

abstract：:Heterotrimeric G proteins typically transduce signals from G protein-coupled receptors (GPCRs) to effector proteins. In the conventional G protein signaling paradigm, the G protein is located at the cytoplasmic surface of the plasma membrane, where, after activation by an agonist-bound GPCR, the GTP-bound Gα and free ...

journal_title：Cellular signalling

authors： Hewavitharana T,Wedegaertner PB

更新日期：2012-01-01 00:00:00

abstract：:Obesity is a widespread health problem that brings about various adipose tissue dysfunctions. The balance of energy storage and energy expenditure is critical for normal fat accumulation and lipid metabolism. Therefore, understanding the molecular basis of adipogenesis and thermogenesis is essential to maintain adipos...

journal_title：Cellular signalling

authors： Chen C,Cui Q,Zhang X,Luo X,Liu Y,Zuo J,Peng Y

更新日期：2018-11-01 00:00:00

abstract：:Cortical and medullary thymocytes can be separated from each other by virtue of the fact that only cortical thymocytes bear peanut agglutinin (PNA) receptors. The mitogenic responses of subpopulations of thymocytes were studied. We have confirmed the results of Conlon et al. [(1982) J. Immun. 128, 797-801], that lecti...

journal_title：Cellular signalling

authors： Zilberman Y

更新日期：1990-01-01 00:00:00

abstract：:Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 c...

journal_title：Cellular signalling

authors： Fink D,Contreras ML,Lelkes PI,Lazarovici P

更新日期：1989-01-01 00:00:00

abstract：:The Reelin-Disabled 1 (Dab1) signaling pathway plays an important role in neuronal cell migration during brain development. Dab1, an intracellular adapter protein which is tyrosine phosphorylated upon Reelin stimulation, has been directly implicated in the transmission and termination of Reelin-mediated signaling. Two...

journal_title：Cellular signalling

authors： Gao Z,Godbout R

更新日期：2011-03-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) define a specific group of eukaryotic protein kinases which regulate a number of cellular functions by transducing extracellular signals to intracellular responses. Unlike other protein kinases, catalytic activation of MAPKs by MAPKKs depends on dual phosphorylation at two tyr...

journal_title：Cellular signalling

authors： Vázquez B,Soto T,del Dedo JE,Franco A,Vicente J,Hidalgo E,Gacto M,Cansado J,Madrid M

更新日期：2015-12-01 00:00:00

abstract：:Netrin-1 attracts or repels growing axons during development. The UNC5 receptors mediate the repulsive response, either alone or in complex with DCC receptors. The signaling mechanisms activated by UNC5 are poorly understood. Here, we examined the role of Rho GTPases in UNC5a signaling. We found that UNC5a induced neu...

journal_title：Cellular signalling

authors： Picard M,Petrie RJ,Antoine-Bertrand J,Saint-Cyr-Proulx E,Villemure JF,Lamarche-Vane N

更新日期：2009-12-01 00:00:00

abstract：:Protein phosphatases play a key role in balancing the cellular responses to the transforming growth factor-β (TGFβ) signals. Several protein phosphatases have been attributed roles in the regulation of the TGFβ pathway. Among these, PPM1A is the only phosphatase reported to dephosphorylate SMAD2/3 in the nucleus. Howe...

journal_title：Cellular signalling

authors： Bruce DL,Macartney T,Yong W,Shou W,Sapkota GP

更新日期：2012-11-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase cascades are signal transduction pathways that play pivotal regulatory roles in the biosynthesis of pro-inflammatory cytokines. MAP kinase phosphatase (MKP)-1, an archetypal member of the MKP family, is essential for the dephosphorylation/deactivation of MAP kinases p38 and JNK. ...

journal_title：Cellular signalling

authors： Wang X,Liu Y

更新日期：2007-07-01 00:00:00

abstract：:Lysophosphatidic acid (LPA) plays a critical role in the pathophysiology of ovarian cancers. Previous studies have shown that LPA stimulates the proliferation of ovarian cancer cells via Gα12. The present study utilizing Protein/DNA array analyses of LPA-stimulated HeyA8 cells in which the expression of Gα12 was silen...

journal_title：Cellular signalling

authors： Ha JH,Ward JD,Varadarajalu L,Kim SG,Dhanasekaran DN

更新日期：2014-01-01 00:00:00

abstract：:We assign a new function to a tumor suppressor NPRL2 that activates the mTOR complex 1 (mTORC1) activity. The positive regulation of mTORC1 activity by NPRL2 is mediated through NPRL2 interaction with Raptor. While NPRL2 interacts with Rag GTPases, RagD in particular, to interfere with mTORC1 activity in amino acid sc...

journal_title：Cellular signalling

authors： Kwak SS,Kang KH,Kim S,Lee S,Lee JH,Kim JW,Byun B,Meadows GG,Joe CO

更新日期：2016-02-01 00:00:00

abstract：:As a member of the Rab small GTPase family, Rab11a has been shown to be involved in different vesicle trafficking processes. In earlier work we identified Rab11a to be present in GLUT4-containing vesicles after insulin stimulation and showed its involvement in insulin-dependent glucose uptake. However, it remained elu...

journal_title：Cellular signalling

authors： Schwenk RW,Eckel J

更新日期：2007-04-01 00:00:00

abstract：:Suppressor of Fused (SuFu) plays a conservative role in the regulation of the Gli transcription factors within the Hedgehog (Hh) signaling pathway. Despite the central importance of SuFu in the Hh pathway, little is known about its regulation. Here, we performed a GAL4-based yeast two-hybrid screen using human SuFu as...

journal_title：Cellular signalling

authors： Wang Y,Li Y,Hu G,Huang X,Rao H,Xiong X,Luo Z,Lu Q,Luo S

更新日期：2016-09-01 00:00:00

abstract：:The mitotic inhibitor securin is degraded via the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C)-Cdc20 after anaphase onset. This triggers activation of the mitotic protease separase and thereby sister chromatid separation. However, only a proportion of securin molecules are degraded at metaphase-anapha...

journal_title：Cellular signalling

authors： Hatano Y,Naoki K,Suzuki A,Ushimaru T

更新日期：2016-10-01 00:00:00

abstract：:Tyrosine phosphorylation of proteins is a mechanism of signalling for different receptors and is important for cell growth and differentiation. Mast cells and basophils are secretory cells that play a role in inflammatory and immediate allergic reactions. The activation/aggregation of different surface receptors on th...

journal_title：Cellular signalling

authors： Hamawy MM,Mergenhagen SE,Siraganian RP

更新日期：1995-08-01 00:00:00

abstract：:CyrA is a novel cysteine-rich protein with four EGFL repeats that was isolated using the calmodulin (CaM) binding overlay technique (CaMBOT), suggesting it is a CaM-binding protein (CaMBP). The full-length 63kDa cyrA is cleaved into two major C-terminal fragments, cyrA-C45 and cyrA-C40. A putative CaM-binding domain w...

journal_title：Cellular signalling

authors： Suarez A,Huber RJ,Myre MA,O'Day DH

更新日期：2011-07-01 00:00:00

abstract：:The Wnt signalling pathway is conserved in various species from worms to mammals, and plays important roles in development, cellular proliferation, and differentiation. The molecular mechanisms by which the Wnt signal regulates cellular functions are becoming increasingly well understood. Wnt stabilizes cytoplasmic be...

journal_title：Cellular signalling

authors： Kikuchi A

更新日期：1999-11-01 00:00:00

abstract：:Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairments. The classical symptoms of the disease include gradual deterioration of memory and language. Epidemiological studies indicate that around 25-40% of AD patients have sleep-wake cycle disturbances. Importantly, a series of st...

journal_title：Cellular signalling

authors： Havekes R,Heckman PRA,Wams EJ,Stasiukonyte N,Meerlo P,Eisel ULM

更新日期：2019-12-01 00:00:00

abstract：:We have demonstrated previously that chronic exposure to endothlin-1 enhances glucose transport in 3T3-L1 adipocytes via augmented GLUT1 mRNA and protein accumulation. In the present study, we further examined the combined effect of endothelin-1 (ET-1) and cAMP on glucose transport. In cells pretreated with ET-1 and 8...

journal_title：Cellular signalling

authors： Fong JC,Kao YS,Tsai HY,Chiou YY,Chiou GY

更新日期：2004-07-01 00:00:00

abstract：:Insulin is an inducer of brown fat adipogenesis through the activation of a signalling network that involves positive/negative modulators. Given the importance of brown adipose tissue (BAT) for basal thermogenic energy expenditure, we investigated the role of PTP1B in the acquisition of terminal differentiated phenoty...

journal_title：Cellular signalling

authors： Miranda S,González-Rodríguez A,Revuelta-Cervantes J,Rondinone CM,Valverde AM

更新日期：2010-04-01 00:00:00