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Phosphorylation of serine 323 of ASB2α is pivotal for the targeting of filamin A to degradation.

Abstract：

:ASB proteins are the specificity subunits of cullin5-RING E3 ubiquitin ligases (CRL5) that play roles in ubiquitin-mediated protein degradation. However, how their activity is regulated remains poorly understood. Here, we unravel a novel mechanism of regulation of a CRL5 through phosphorylation of its specificity subunit ASB2α. Indeed, using mass spectrometry, we showed for the first time that ASB2α is phosphorylated and that phosphorylation of serine-323 (Ser-323) of ASB2α is crucial for the targeting of the actin-binding protein filamin A (FLNa) to degradation. Mutation of ASB2α Ser-323 to Ala had no effect on intrinsic E3 ubiquitin ligase activity of ASB2α but abolished the ability of ASB2α to induce degradation of FLNa. In contrast, the ASB2α Ser-323 to Asp phosphomimetic mutant induced acute degradation of FLNa. Moreover, inhibition of the extracellular signal-regulated kinases 1 and 2 (Erk1/2) activity reduced ASB2α-mediated FLNa degradation. We further showed that the subcellular localization of ASB2α to actin-rich structures is dependent on ASB2α Ser-323 phosphorylation and propose that the interaction with FLNa depends on the electrostatic potential redistribution induced by the Ser-323 phosphate group. Taken together, these data unravel an important mechanism by which ASB2α-mediated FLNa degradation can be regulated.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Zakaria R,Lamsoul I,Uttenweiler-Joseph S,Erard M,Monsarrat B,Burlet-Schiltz O,Moog-Lutz C,Lutz PG

doi

10.1016/j.cellsig.2013.09.011

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

2823-30

issue

12

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(13)00291-X

journal_volume

25

pub_type

杂志文章

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