Abstract:
:Interferon-α (IFNα) has enormous potential for anti-proliferative and anti-viral treatments. However, clinical success is still hampered due to its limited bioavailability and thus, lack of sustained modulation of disease-relevant protective programs. Consequently, we here examined whether IFNα immobilized on nanoscale ferromagnetic R-Chitosan carriers is capable of inducing rapid and sustained activation of STAT1 signaling. We report the spontaneous formation of a stable nanoparticle-IFNα protein corona, which was exploited to generate IFNα-loaded spheres, obviating the need to specifically couple the cytokine to the nanoparticles (NPs). Notably, comprehensive experimental approaches ensure that formation of the IFNα NP-corona does not affect the biological activity of the cytokine, as STAT1 signaling was efficiently activated. Employing human prostate cancer and melanoma cell models, we found that the intensity and duration of STAT1 phosphorylation as well as the downstream activation of pathobiologically relevant genes were dose and particle dependent. In comparison with free IFNα, IFNα-loaded spheres resulted in a more sustained biologically relevant STAT1 activation, demonstrated also by conferring innate cellular immunity against vesicular stomatitis virus (VSV) infection. For one, our study demonstrates the advantages of biodegradable IFNα-coated R-Chitosan NPs for controlled cytokine release, and thereby improved therapy. Second, we reveal that the permanent presence of IFNα and not just the initial STAT1 phosphorylation ensures sustained IFNα-dependent signaling.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Pollok S,Ginter T,Günzel K,Pieper J,Henke A,Stauber RH,Reichardt W,Krämer OHdoi
10.1016/j.cellsig.2013.01.012subject
Has Abstractpub_date
2013-04-01 00:00:00pages
989-98issue
4eissn
0898-6568issn
1873-3913pii
S0898-6568(13)00015-6journal_volume
25pub_type
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2011.03.014
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2011.06.022
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2018.12.005
更新日期:2019-02-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2010.10.017
更新日期:2011-02-01 00:00:00
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2006.02.008
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2010.05.003
更新日期:2010-09-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2012.06.007
更新日期:2012-10-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2011.09.022
更新日期:2012-02-01 00:00:00