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Effects of cholinergic agonists on diacylglycerol and intracellular calcium levels in pancreatic beta-cells.

Abstract：

:We have studied the effects of cholinergic agonists on the rates of insulin release and the concentrations of diacylglycerol (DAG) and intracellular free Ca2+ ([Ca2+]i) in the beta-cell line MIN6. Insulin secretion was stimulated by glucose, by glibenclamide and by bombesin. In the presence of glucose, both acetylcholine (ACh) and carbachol (CCh) produced a sustained increase in the rate of insulin release which was blocked by EGTA or verapamil. The DAG content of MIN6 beta-cells was not affected by glucose. Both CCh and ACh evoked an increase in DAG which was maximal after 5 min and returned to basal after 30 min; EGTA abolished the cholinergic-induced increase in DAG. ACh caused a transient rise in [Ca2+]i which was abolished by omission of Ca2+ or by addition of devapamil. Thus, cholinergic stimulation of beta-cell insulin release is associated with changes in both [Ca2+]i and DAG. The latter change persists longer than the former and activation of protein kinase C and sensitization of the secretory process to Ca2+ may underlie the prolonged effects of cholinergic agonists on insulin release. However, a secretory response to CCh was still evident after both [Ca2+]i and DAG had returned to control values suggesting that additional mechanisms may be involved.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Weng L,Davies M,Ashcroft SJ

doi

10.1016/0898-6568(93)90038-n

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

777-86

issue

6

eissn

0898-6568

issn

1873-3913

pii

0898-6568(93)90038-N

journal_volume

5

pub_type

杂志文章

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