Protein tyrosine phosphorylation as a mechanism of signalling in mast cells and basophils.

abstract：:In humans, thromboxane (TX) A₂ signals through the TPα and TPβ isoforms of its G-protein coupled TXA₂ receptor (TP) to mediate a host of (patho)physiologic responses. Herein, angio-associated migratory cell protein (AAMP) was identified as a novel interacting partner of both TPα and TPβ through an interaction dependen...

journal_title：Cellular signalling

authors： Reid HM,Wikström K,Kavanagh DJ,Mulvaney EP,Kinsella BT

更新日期：2011-04-01 00:00:00

abstract：:In this study, we showed that nitric oxide (NO) donors induced the mesangial cell proliferation and cyclooxygenase-2 (COX-2) protein expression in murine mesangial cells. An inflammatory condition [lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma)] could also induce cell proliferation and significantly enhanc...

journal_title：Cellular signalling

authors： Sheu ML,Chao KF,Sung YJ,Lin WW,Lin-Shiau SY,Liu SH

更新日期：2005-08-01 00:00:00

abstract：:The duration of ERK1/2 activation influences the nature of the biological response to agonist. Members of the AP-1 transcription factor family are well known targets of the ERK1/2 pathway and are expressed in a temporally coordinated fashion during cell cycle re-entry. In CCl39 fibroblasts, sustained ERK1/2 activation...

journal_title：Cellular signalling

authors： Chalmers CJ,Gilley R,March HN,Balmanno K,Cook SJ

更新日期：2007-04-01 00:00:00

abstract：:In this study, we demonstrate that EGF inhibits the TGF-β1-induced apoptosis of Huh7 cells. TGF-β1 up-regulates the expression of PDCD4 causing apoptosis, by stimulating the synthesis of PDCD4 mRNA via the Smad signaling pathway. TGF-β1 also inhibits the activation of S6 kinase 1 which phosphorylates the serine 67 res...

journal_title：Cellular signalling

authors： Matsuhashi S,Hamajima H,Xia J,Zhang H,Mizuta T,Anzai K,Ozaki I

更新日期：2014-03-01 00:00:00

abstract：:Ephedrine (Eph) is one of alkaloids that has been isolated from the ancient herb ephedra (ma huang) and is used as the treatment of asthma, hypotension and fatigue. However, its molecular mechanism remains unknown. Thioredoxin-1 (Trx-1) is a redox regulating protein, which has various biological activities, including ...

journal_title：Cellular signalling

authors： Jia JJ,Zeng XS,Li Y,Ma S,Bai J

更新日期：2013-05-01 00:00:00

abstract：:The present study was designed to investigate (i) the role of AMPK activation in inducing autophagy in androgen-dependent prostate cancer cells subjected to androgen deprivation and hypoxia, and (ii) whether autophagy offers a survival advantage under these harsh conditions. Low androgen and low oxygen are two co-exis...

journal_title：Cellular signalling

authors： Chhipa RR,Wu Y,Ip C

更新日期：2011-09-01 00:00:00

abstract：:Human neutrophils pre-incubated with granulocyte-macrophage-colony-stimulating factor (GM-CSF) exhibit an enhanced mobilization of calcium in response to secondary stimuli such as chemotactic factors. The mechanisms underlying this priming effect of GM-CSF were examined. It was first demonstrated that the additional c...

journal_title：Cellular signalling

authors： Naccache PH,Hamelin B,Gaudry M,Bourgoin S

更新日期：1991-01-01 00:00:00

abstract：:Heterotrimeric (alphabetagamma) G(s) mediates agonist-induced stimulation of adenylyl cyclase (AC). Cholera toxin (CTx) will ADP-ribosylate the alpha-subunit of G(s) (G(s)alpha). G(s)alpha-deficient cyc(-) membranes were "stripped" of Gbeta. When the stripped cyc(-) were incubated with G(s)alpha and/or Gbetagamma, eac...

journal_title：Cellular signalling

authors： Ganpat MM,Nishimura M,Toyoshige M,Okuya S,Pointer RH,Rebois RV

更新日期：2000-02-01 00:00:00

abstract：:15-lipoxygenase (15-LO) is known to play an important role in chronic pulmonary hypertension. Accumulating evidence for its down-stream participants in the vasoconstriction and remodeling processes of pulmonary arteries, while how hypoxia regulates 15-LO/15-hydroxyeicosatetraenoic acid (15-HETE) to mediate hypoxic pul...

journal_title：Cellular signalling

authors： Zhang L,Ma J,Shen T,Wang S,Ma C,Liu Y,Ran Y,Wang L,Liu L,Zhu D

更新日期：2012-10-01 00:00:00

abstract：:The possible involvement of zeta isozyme of protein kinase C (PKC zeta) in phorbol ester-induced signal transduction was investigated in mouse epidermal cells. Western blot analysis of RESOURCE Q column chromatography eluates obtained from 105,000 g supernatants and particulate fractions of epidermal cells was perform...

journal_title：Cellular signalling

authors： Nishikawa K,Yamamoto S,Nagumo H,Maruyama K,Kato R

更新日期：1995-07-01 00:00:00

abstract：:Nuclear factor of activated T cells (NFAT) plays a prominent role in gene transcription during the immune response. Growing evidence demonstrates the implication of inducible phosphorylation of the transactivation domain (TAD) of NFAT in transcriptional activation of genes. We have analyzed the regulation of NFATc2 ac...

journal_title：Cellular signalling

authors： Gómez-Casero E,San-Antonio B,Iñiguez MA,Fresno M

更新日期：2007-08-01 00:00:00

abstract：:Recycling of the majority of agonist-internalized GPCR is dependent on a type I-PDZ "barcode" in their C-tail. The recycling of wild-type (WT) ß1-AR is also dependent on its default "type-1 PDZ barcode", but trafficking of the ß1-AR is inhibited when PKA or its substrate serine at position 312 (Ser312) are inactivated...

journal_title：Cellular signalling

authors： Nooh MM,Bahouth SW

更新日期：2017-01-01 00:00:00

abstract：:Rho GTPases are molecular switches involved in the regulation of many cellular processes. This review summarizes work examining how stimulation of receptor tyrosine kinases (RTKs) leads to the activation of Rho guanine nucleotide exchange factors (GEFs) and their Rho GTPase substrates. The collective findings strongly...

journal_title：Cellular signalling

authors： Schiller MR

更新日期：2006-11-01 00:00:00

abstract：:The adaptor protein Gab-2 coordinates the assembly of the IL-3 signalsome comprising Gab-2, Grb2, Shc, SHP-2 and PI3K. To investigate the role of the pleckstrin homology domain of Gab-2 in this process, epitope-tagged wild type Gab-2 (WTGab-2), Gab-2 lacking its PH domain (DeltaPHGab-2) and the Gab-2 PH domain alone (...

journal_title：Cellular signalling

authors： Edmead CE,Fox BC,Stace C,Ktistakis N,Welham MJ

更新日期：2006-08-01 00:00:00

abstract：:We report here a direct modulation by mast cell tryptase of endothelial barrier function through activation of proteinase-activated receptor-2 (PAR-2). In cultured bovine aortic endothelial cells (BAECs), tryptase, trypsin and PAR-2 activating peptide impaired the barrier function as determined by the permeability of ...

journal_title：Cellular signalling

authors： Sendo T,Sumimura T,Itoh Y,Goromaru T,Aki K,Yano T,Oike M,Ito Y,Mori S,Nishibori M,Oishi R

更新日期：2003-08-01 00:00:00

abstract：:Cyclic mechanical stretch associated with pulsatile blood pressure can modulate cytoskeletal remodeling and intracellular signaling in vascular endothelial cells. The aim of this study was to evaluate the role of stretch-induced actin stress fiber orientation in intracellular signaling involving the activation of c-ju...

journal_title：Cellular signalling

authors： Kaunas R,Usami S,Chien S

更新日期：2006-11-01 00:00:00

abstract：:The CD34 protein is regarded as a marker of stem cells from multiple origins. Recently a mesenchymal progenitor CD34 positive cell identified from traumatized human skeletal muscle demonstrates differentiation capability into vascular endothelial cells, osteoblasts and adipocytes. Here they were treated with a small i...

journal_title：Cellular signalling

authors： Wolcott KM,Woodard GE

更新日期：2020-10-01 00:00:00

abstract：:p130Cas/breast cancer anti-oestrogen resistance 1 (BCAR1) is a member of the Cas (Crk-associated substrate) family of adaptor proteins, which have emerged as key signalling nodes capable of interactions with multiple proteins, with important regulatory roles in normal and pathological cell function. The Cas family of ...

journal_title：Cellular signalling

authors： Barrett A,Pellet-Many C,Zachary IC,Evans IM,Frankel P

更新日期：2013-04-01 00:00:00

abstract：:Macrophages take up oxidized low density lipoprotein (oxLDL) after being exposed to it in the blood vessels. oxLDL transforms macrophages into foam cells, which are a hallmark of atherosclerosis. The effects that oxLDL have on the inflammatory responses of foam cells are not clear. Here, we investigated how oxLDL modu...

journal_title：Cellular signalling

authors： Min KJ,Cho KH,Kwon TK

更新日期：2012-06-01 00:00:00

abstract：:Insulin/IGF-1 action is driven by a complex and highly integrated signalling network. Loss-of-function studies indicate that the major insulin/IGF-1 receptor substrate (IRS) proteins, IRS-1 and IRS-2, mediate different biological functions in vitro and in vivo, suggesting specific signalling properties despite their h...

journal_title：Cellular signalling

authors： Rabiee A,Krüger M,Ardenkjær-Larsen J,Kahn CR,Emanuelli B

更新日期：2018-07-01 00:00:00

abstract：:Neuroprotective effects of alpha(2)-adrenergic receptor (AR) agonists are mediated via the alpha(2A)AR subtype, but the molecular mechanisms underlying these actions are still not elucidated. A two-hybrid screen was performed to identify new proteins that may control alpha(2)AR receptor function and trafficking. This ...

journal_title：Cellular signalling

authors： Weber B,Schaper C,Wang Y,Scholz J,Bein B

更新日期：2009-10-01 00:00:00

abstract：:The roles of protein kinase C (PKC) isoenzymes in the differentiation process of THP-1 cells are investigated. Inhibition of PKC by RO 31-8220 reduces the phagocytosis of latex particles and the release of superoxide, prostaglandin E(2) (PGE(2)), and tumour necrosis factor (TNF)-alpha. The proliferation of THP-1 cells...

journal_title：Cellular signalling

authors： Dieter P,Schwende H

更新日期：2000-05-01 00:00:00

abstract：:Using adenoviruses encoding RGS2, RGS4 and Lsc (regulator of G protein signalling (RGS) domain of p115 RhoGEF), we investigated the contributions of G(q/11), Gi and G(12/13) proteins to G protein-coupled receptor (GPCR)-mediated activation of the extracellular signal-regulated kinase (ERK) pathway in adult rat ventric...

journal_title：Cellular signalling

authors： Snabaitis AK,Muntendorf A,Wieland T,Avkiran M

更新日期：2005-05-01 00:00:00

abstract：:Our previous oligonucleotide array studies revealed that ALK-positive anaplastic large cell lymphoma (ALK(+)ALCL) express high levels of the disheveled proteins (Dvls), a family of proteins that is integral to the Wnt signaling pathways. In this study, we assessed whether the Dvls are important in the pathogenesis of ...

journal_title：Cellular signalling

authors： Hegazy SA,Alshareef A,Gelebart P,Anand M,Armanious H,Ingham RJ,Lai R

更新日期：2013-01-01 00:00:00

abstract：:The strong inhibition of thrombin-induced platelet functions induced by okadaic acid is not correlated with the partial modification of pleckstrin phosphorylation, which remains still phosphorylated two min after stimulation, indicating that protein kinase C is not affected by okadaic acid. We then investigated the ef...

journal_title：Cellular signalling

authors： Mauco G,Artçanuthurry V,Pidard D,Grelac F,Maclouf J,Lévy-Tolédano S

更新日期：1997-01-01 00:00:00

abstract：:Several members of the mitogen-activated protein kinase kinase kinase (MAP3K) family including MEKK3 and TGFbeta-activating kinase (TAK1) play nonredundant roles in activation of the NF-kappaB transcription factor. However, the mechanism by which MEKK3 mediates NF-kappaB signaling is not fully understood. In this repo...

journal_title：Cellular signalling

authors： Di Y,Li S,Wang L,Zhang Y,Dorf ME

更新日期：2008-04-01 00:00:00

abstract：:We have demonstrated previously that chronic exposure to endothlin-1 enhances glucose transport in 3T3-L1 adipocytes via augmented GLUT1 mRNA and protein accumulation. In the present study, we further examined the combined effect of endothelin-1 (ET-1) and cAMP on glucose transport. In cells pretreated with ET-1 and 8...

journal_title：Cellular signalling

authors： Fong JC,Kao YS,Tsai HY,Chiou YY,Chiou GY

更新日期：2004-07-01 00:00:00

abstract：:Regulators of G protein signalling (RGS) proteins are united into a family by the presence of the RGS domain which serves as a GTPase-activating protein (GAP) for various Galpha subunits of heterotrimeric G proteins. Through this mechanism, RGS proteins regulate signalling of numerous G protein-coupled receptors. In a...

journal_title：Cellular signalling

authors： Sethakorn N,Yau DM,Dulin NO

更新日期：2010-09-01 00:00:00

abstract：:We report on a novel method to monitor changes in intracellular cAMP concentration ([cAMP]i) within intact living cells using a chimeric fusion of the catalytic subunit of cAMP-dependent protein kinase to green fluorescent protein (PKAcat-GFP). In stably transfected unstimulated fibroblasts, fusion protein fluorescenc...

journal_title：Cellular signalling

authors： Almholt K,Tullin S,Skyggebjerg O,Scudder K,Thastrup O,Terry R

更新日期：2004-08-01 00:00:00

abstract：:In order to elucidate the molecular events in signal transduction, examination of the interaction between Ras and Raf-1 seems crucial. Many Raf-1 mutants have been investigated in terms of their binding activities to Ras, where only a few Ras mutants have been examined thus far. We have investigated the Raf-1-binding ...

journal_title：Cellular signalling

authors： Hiwasa T,Kasama M,Nakadai T,Sawada T,Sakiyama S

更新日期：1996-08-01 00:00:00