Involvement of proteinase-activated receptor-2 in mast cell tryptase-induced barrier dysfunction in bovine aortic endothelial cells.

Abstract:

:We report here a direct modulation by mast cell tryptase of endothelial barrier function through activation of proteinase-activated receptor-2 (PAR-2). In cultured bovine aortic endothelial cells (BAECs), tryptase, trypsin and PAR-2 activating peptide impaired the barrier function as determined by the permeability of protein-conjugated Evans blue. The tryptase-induced barrier dysfunction was completely blocked by U73122, and partially reversed by xestospongin C, calphostin C or Y27632. The intracellular Ca(2+) was elevated by tryptase. It was notable that ioxaglate, a contrast material that degranulates mast cells, markedly increased the permeability when applied to BAECs in combination with mast cells, an action that was blocked by nafamostat, a potent tryptase inhibitor. Immunofluorescence analysis showed that actin stress fibre formation and disruption of VE-cadherin were observed after exposure to tryptase or ioxaglate in combination with mast cells. Therefore, it is suggested that mast cell tryptase impairs endothelial barrier function through activation of endothelial PAR-2 in a manner dependent on the phospholipase C activity.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Sendo T,Sumimura T,Itoh Y,Goromaru T,Aki K,Yano T,Oike M,Ito Y,Mori S,Nishibori M,Oishi R

doi

10.1016/s0898-6568(03)00014-7

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

773-81

issue

8

eissn

0898-6568

issn

1873-3913

pii

S0898656803000147

journal_volume

15

pub_type

杂志文章
  • The shape of things to come: an emerging role for protein kinase CK2 in the regulation of cell morphology and the cytoskeleton.

    abstract::Protein kinase CK2 is a highly conserved, pleiotropic, protein serine/threonine kinase that is essential for life in eukaryotes. CK2 has been implicated in diverse cellular processes such as cell cycle regulation, circadian rhythms, apoptosis, transformation and tumorigenesis. In addition, there is increasing evidence...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2005.07.008

    authors: Canton DA,Litchfield DW

    更新日期:2006-03-01 00:00:00

  • Redox control of p53 in the transcriptional regulation of TGF-β1 target genes through SMAD cooperativity.

    abstract::Transforming growth factor-β1 (TGF-β1) regulates the tissue response to injury and is the principal driver of excessive scarring leading to fibrosis and eventual organ failure. The TGF-β1 effectors SMAD3 and p53 are major contributors to disease progression. While SMAD3 is an established pro-fibrotic factor, the role ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.02.017

    authors: Overstreet JM,Samarakoon R,Meldrum KK,Higgins PJ

    更新日期:2014-07-01 00:00:00

  • Role of glycosyl-phosphatidylinositol hydrolysis as a mitogenic signal for epidermal growth factor.

    abstract::We have investigated the role of the hydrolysis of glycosyl-phosphatidylinositol (GPI) as one of the signalling pathways elicited after interaction of epidermal growth factor (EGF) with its specific plasma membrane receptor (EGFR). Endogenous GPI was characterized in both NIH 3T3 mouse fibroblast cells and in EGFR-tra...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(95)00002-7

    authors: Clemente R,Jones DR,Ochoa P,Romero G,Mato JM,Varela-Nieto I

    更新日期:1995-05-01 00:00:00

  • Adenosine A1 receptors inhibit both adenylate cyclase activity and TRH-activated Ca2+ channels by a pertussis toxin-sensitive mechanism in GH3 cells.

    abstract::The present study has examined the effects of adenosine A1 receptors on second messenger processes in GH3 cells. A1 receptors are present which are shown to inhibit adenylate cyclase in a GTP-requiring manner. Hormone (VIP) stimulation is also absolutely required for the observation of inhibition. Adenosine A1 recepto...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(89)90023-5

    authors: Cooper DM,Caldwell KK,Boyajian CL,Petcoff DW,Schlegel W

    更新日期:1989-01-01 00:00:00

  • A caffeine/ryanodine-sensitive Ca2+ pool is involved in triggering spontaneous variations of Ca2+ in Jurkat T lymphocytes by a Ca(2+)-induced Ca2+ release (CICR) mechanism.

    abstract::Caffeine and ryanodine triggered an increase in [Ca2+]i (73 +/- 22 and 61 +/- 18 nM, respectively) in Jurkat cell populations that was independent of external Ca2+. In individual cells, caffeine and ryanodine induced Ca2+ spikes. Jurkat cell populations initially exposed to caffeine did not respond further to ryanodin...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(96)00141-6

    authors: Ricard I,Martel J,Dupuis L,Dupuis G,Payet MD

    更新日期:1997-02-01 00:00:00

  • The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.

    abstract::Macrophages take up oxidized low density lipoprotein (oxLDL) after being exposed to it in the blood vessels. oxLDL transforms macrophages into foam cells, which are a hallmark of atherosclerosis. The effects that oxLDL have on the inflammatory responses of foam cells are not clear. Here, we investigated how oxLDL modu...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.02.001

    authors: Min KJ,Cho KH,Kwon TK

    更新日期:2012-06-01 00:00:00

  • Regulation of alternative splicing of CD44 in cancer.

    abstract::CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce vario...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2014.07.011

    authors: Prochazka L,Tesarik R,Turanek J

    更新日期:2014-10-01 00:00:00

  • Phorbol ester effects on hormonal responses in freshly isolated short-term incubated and cultured hepatocytes.

    abstract::Beta-adrenergic, alpha-1-adrenergic and glucagon stimulation of glucose release were compared between hepatocytes which were freshly isolated, incubated for 3 h in suspension or cultivated for 4 or 24 h in plastic culture flasks in the presence and absence of the protein kinase C activator 12-O-tetradecanoylphorbol-13...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(92)90051-9

    authors: Hermsdorf T,Dettmer D

    更新日期:1992-11-01 00:00:00

  • Genomic analysis of G protein gamma subunits in human and mouse - the relationship between conserved gene structure and G protein betagamma dimer formation.

    abstract::Analysis of the genomic sequences, cDNAs and expressed sequence tags (ESTs) in human and mouse for the 12 genes of the gamma subunits of the heterotrimeric G proteins has allowed us to identify the common versus unique elements of the organization and expression of the members of this important gene family. All of the...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2005.04.011

    authors: Yang W,Hildebrandt JD

    更新日期:2006-02-01 00:00:00

  • Investigate the role of PTEN in chemotaxis of human breast cancer cells.

    abstract::Chemotaxis plays an important role in metastasis of cancer cells. In the current study, we investigated the role of PTEN, a tumor suppressor, in chemotaxis of human breast cancer cells. Over-expression of PTEN inhibited EGF-induced chemotaxis, probably due to an overall reduction of PIP(3) levels. Disruption of PTEN b...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2007.06.007

    authors: Wan W,Zou H,Sun R,Liu Y,Wang J,Ma D,Zhang N

    更新日期:2007-11-01 00:00:00

  • Frizzled7 as an emerging target for cancer therapy.

    abstract::Wnt proteins are secreted glycoproteins that bind to the N-terminal extra-cellular cysteine-rich domain of the Frizzled (Fzd) receptor family. The Fzd receptors can respond to Wnt proteins in the presence of Wnt co-receptors to activate the canonical and non-canonical Wnt pathways. Recent studies indicated that, among...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2011.12.009

    authors: King TD,Zhang W,Suto MJ,Li Y

    更新日期:2012-04-01 00:00:00

  • Regulation of the extracellular signal-regulated kinase pathway in adult myocardium: differential roles of G(q/11), Gi and G(12/13) proteins in signalling by alpha1-adrenergic, endothelin-1 and thrombin-sensitive protease-activated receptors.

    abstract::Using adenoviruses encoding RGS2, RGS4 and Lsc (regulator of G protein signalling (RGS) domain of p115 RhoGEF), we investigated the contributions of G(q/11), Gi and G(12/13) proteins to G protein-coupled receptor (GPCR)-mediated activation of the extracellular signal-regulated kinase (ERK) pathway in adult rat ventric...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2004.10.008

    authors: Snabaitis AK,Muntendorf A,Wieland T,Avkiran M

    更新日期:2005-05-01 00:00:00

  • In resting COS1 cells a dominant negative approach shows that specific, anchored PDE4 cAMP phosphodiesterase isoforms gate the activation, by basal cyclic AMP production, of AKAP-tethered protein kinase A type II located in the centrosomal region.

    abstract::We employ a novel, dominant negative approach to identify a key role for certain tethered cyclic AMP specific phosphodiesterase-4 (PDE4) isoforms in regulating cyclic AMP dependent protein kinase A (PKA) sub-populations in resting COS1 cells. A fraction of PKA is clearly active in resting COS1 cells and this activity ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2005.04.003

    authors: McCahill A,McSorley T,Huston E,Hill EV,Lynch MJ,Gall I,Keryer G,Lygren B,Tasken K,van Heeke G,Houslay MD

    更新日期:2005-09-01 00:00:00

  • Symmetric dimethylarginine alters endothelial nitric oxide activity in glomerular endothelial cells.

    abstract::Circulating symmetric dimethylarginine (SDMA) is increased in patients with chronic kidney disease. SDMA is considered an inert metabolite, but because it can transported into cells, we studied the effect of SDMA on glomerular endothelial cells. SDMA suppressed VEGF-induced endothelial nitric oxide synthase (eNOS) pho...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.09.024

    authors: Feliers D,Lee DY,Gorin Y,Kasinath BS

    更新日期:2015-01-01 00:00:00

  • TMPRSS4 upregulates uPA gene expression through JNK signaling activation to induce cancer cell invasion.

    abstract::TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed in pancreatic, thyroid, colon, and other cancer tissues. Previously, we demonstrated that TMPRSS4 mediates tumor cell invasion, migration, and metastasis. However, the mechanisms by which TMPRSS4 contributes to invasion are not fully und...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.08.002

    authors: Min HJ,Lee Y,Zhao XF,Park YK,Lee MK,Lee JW,Kim S

    更新日期:2014-02-01 00:00:00

  • Nuclear presence of nuclear factor of activated T cells (NFAT) c3 and c4 is required for Toll-like receptor-activated innate inflammatory response of monocytes/macrophages.

    abstract::Nuclear factor of activated T cells (NFATs) are crucial transcription factors that tightly control proinflammatory cytokine expression for adaptive immunity in T and B lymphocytes. However, little is known about the role of NFATs for innate immunity in macrophages. In this study, we report that NFAT is required for To...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2011.06.013

    authors: Minematsu H,Shin MJ,Celil Aydemir AB,Kim KO,Nizami SA,Chung GJ,Lee FY

    更新日期:2011-11-01 00:00:00

  • PI3K/Akt-dependent phosphorylation of GSK3β and activation of RhoA regulate Wnt5a-induced gastric cancer cell migration.

    abstract::Wnt5a, a non-transforming Wnt family member, plays complicated roles in oncogenesis and cancer metastasis. However, Wnt5a signaling in gastric cancer progression remains poorly defined. In this study, we found that Wnt5a dose-dependently stimulated the migration of human gastric cancer cells (SGC-7901), with the maxim...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.10.012

    authors: Liu J,Zhang Y,Xu R,Du J,Hu Z,Yang L,Chen Y,Zhu Y,Gu L

    更新日期:2013-02-01 00:00:00

  • The effect of lentivirus-mediated SIRT1 gene knockdown in the ATDC5 cell line via inhibition of the Wnt signaling pathway.

    abstract::SIRT1 is a highly conserved type III acetyltransferase gene located on chromosome 10 in mammals that belong to the Sirtuins family. In order to explore the effects of the SIRT1 gene in the ATDC5 cell line, an RNAi SIRT1 target sequence was designed and synthesized, aimed to knockdown the expression of SIRT1 in ATDC5 b...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.09.016

    authors: Yu F,Yuan Y,Li D,Kou Y,Jiang B,Zhang P

    更新日期:2019-01-01 00:00:00

  • Smad2 functions as a co-activator of canonical Wnt/beta-catenin signaling pathway independent of Smad4 through histone acetyltransferase activity of p300.

    abstract::Both canonical Wnt/beta-catenin and TGFbeta/Smad signaling pathways coordinately regulate pattern formation during embryogenesis as well as tumor progression. Evidence of cross-talk between these two pathways has been reported. Here we demonstrated that the Activin-like kinase 4 (Alk4)/Smad2 pathway facilitates the tr...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.05.003

    authors: Hirota M,Watanabe K,Hamada S,Sun Y,Strizzi L,Mancino M,Nagaoka T,Gonzales M,Seno M,Bianco C,Salomon DS

    更新日期:2008-09-01 00:00:00

  • Concentration of enzyme-dependent activation of PLC-beta 1 and PLC-beta 2 by G alpha 11 and beta gamma-subunits.

    abstract::Differential regulation of PLC-beta 1 and -beta 2 by the G-protein alpha-subunit, G alpha 11, and by G-protein beta gamma-subunits was studied utilizing recombinant PLC-beta 1 and -beta 2. Rat PLC-beta 1 and human PLC-beta 2 were purified after recombinant baculovirus-mediated expression in Sf9 cells. The catalytic pr...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(95)00039-r

    authors: Paterson A,Boyer JL,Watts VJ,Morris AJ,Price EM,Harden TK

    更新日期:1995-09-01 00:00:00

  • Upregulated BMP-Smad signaling activity in the glucuronyl C5-epimerase knock out MEF cells.

    abstract::Glucuronyl C5-epimerase (Hsepi) catalyzes the conversion of glucuronic acid to iduronic acid in the process of heparan sulfate biosynthesis. Targeted interruption of the gene, Glce, in mice resulted in neonatal lethality with varied defects in organ development. To understand the underlying molecular mechanisms of the...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.11.010

    authors: Batool T,Fang J,Jansson V,Zhao H,Gallant C,Moustakas A,Li JP

    更新日期:2019-02-01 00:00:00

  • Characteristics of circular RNAs generated by human Survival Motor Neuron genes.

    abstract::Circular RNAs (circRNAs) belong to a diverse class of stable RNAs expressed in all cell types. Their proposed functions include sponging of microRNAs (miRNAs), sequestration and trafficking of proteins, assembly of multimeric complexes, production of peptides, and regulation of transcription. Backsplicing due to RNA s...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2020.109696

    authors: Ottesen EW,Singh RN

    更新日期:2020-09-01 00:00:00

  • Translocation of phospholipase C-gamma 2 induced by in vitro activation of protein tyrosine kinase activity in mast cell lysates.

    abstract::Aggregation of the high-affinity receptor for IgE (Fc eta RI) on the surface of intact or permeabilized rodent mast cells results in tyrosine phosphorylation of phospholipase C-gamma 1 (PLC gamma 1) and PLC gamma 2, and translocation of both isozymes to the particulate fraction. We report here that activation of resid...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(96)00073-3

    authors: Atkinson TP,Yang Q

    更新日期:1996-09-01 00:00:00

  • Differential involvement of H- and K-Ras in Raf-1 activation determines the role of calmodulin in MAPK signaling.

    abstract::We have previously demonstrated that inhibition of calmodulin (CaM) and the concomitant reduction of PI3K interfere with H-Ras-mediated activation of Raf-1 [1]. In the present study, we show that CaM has completely opposite effects on K-Ras-mediated Raf-1 activation. The differential contribution of CaM in the regulat...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.07.018

    authors: Moretó J,Vidal-Quadras M,Pol A,Santos E,Grewal T,Enrich C,Tebar F

    更新日期:2009-12-01 00:00:00

  • Characterization of the isoenzymes of cyclic nucleotide phosphodiesterase in human platelets and the effects of E4021.

    abstract::In extracts of human platelets, three isoenzymes of cyclic nucleotide phosphodiesterase (PDE), namely, PDE2, PDE3, and PDE5, were identified; activities of PDE1 and PDE4 were not detected. In human platelets, the cGMP-hydrolytic activity was about six times higher than the cAMP-hydrolytic activity, and PDE5 and PDE3 a...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(96)00112-x

    authors: Ito M,Nishikawa M,Fujioka M,Miyahara M,Isaka N,Shiku H,Nakano T

    更新日期:1996-12-01 00:00:00

  • Constitutive serum response factor activation by the viral chemokine receptor homologue pUS28 is differentially regulated by Galpha(q/11) and Galpha(16).

    abstract::Expression of the human cytomegalovirus (HCMV)-encoded chemokine receptor homologue pUS28 in mammalian cells results in ligand-dependent and -independent changes in the activity of multiple cellular signal transduction pathways. The ligand-dependent signalling activity of pUS28 has been shown to be predominantly media...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.04.010

    authors: Moepps B,Tulone C,Kern C,Minisini R,Michels G,Vatter P,Wieland T,Gierschik P

    更新日期:2008-08-01 00:00:00

  • Homer1 knockdown protects dopamine neurons through regulating calcium homeostasis in an in vitro model of Parkinson's disease.

    abstract::Homer1 protein is an important scaffold protein at postsynaptic density and has been demonstrated to play a central role in calcium signaling in the central nervous system. The aim of this study was to investigate the effects of Homer1 knockdown on MPP(+) induced neuronal injury in cultured dopamine (DA) neurons. We f...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.09.004

    authors: Chen T,Yang YF,Luo P,Liu W,Dai SH,Zheng XR,Fei Z,Jiang XF

    更新日期:2013-12-01 00:00:00

  • Curcumin represses mTORC1 signaling in Caco-2 cells by a two-sided mechanism involving the loss of IRS-1 and activation of AMPK.

    abstract::The mechanistic target of rapamycin complex 1 (mTORC1) is a central modulator of inflammation and tumorigenesis in the gastrointestinal tract. Growth factors upregulate mTORC1 via the PI3K/AKT and/or Ras/MAPK signal pathways. Curcumin (CUR), a polyphenol found in turmeric roots (Curcuma longa) can repress mTORC1 kinas...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2020.109842

    authors: Kaur H,Moreau R

    更新日期:2021-02-01 00:00:00

  • Differential effect of TPA on PGE2 and cicaprost-induced cAMP synthesis in UMR-106 cells.

    abstract::PGE2 and prostacyclin each enhance cAMP synthesis in the osteoblast-like cell line UMR-106. The amount of cAMP induced by PGE2 was 5-7-fold greater than the amount induced by cicaprost or iloprost, stable prostacyclin analogues. Both PGE2 and the two prostacyclin analogues enhanced cAMP synthesis with similar time dep...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(98)00052-7

    authors: Khanin M,Liel Y,Rimon G

    更新日期:1999-03-01 00:00:00

  • Hsp90/Cdc37 assembly modulates TGFβ receptor-II to act as a profibrotic regulator of TGFβ signaling during cardiac hypertrophy.

    abstract::Cardiac hypertrophy is accompanied by excessive collagen deposition in the heart. Despite painstaking research on this fatal disease, the precise role of molecular chaperones in myocardial fibrosis has not yet been elucidated. In this study, we have analyzed the mechanism by which Heat shock protein 90 (Hsp90)/Cell di...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2015.09.005

    authors: Datta R,Bansal T,Rana S,Datta K,Chattopadhyay S,Chawla-Sarkar M,Sarkar S

    更新日期:2015-12-01 00:00:00