A novel method to monitor insulin-stimulated GTP-loading of Rab11a in cardiomyocytes.


:As a member of the Rab small GTPase family, Rab11a has been shown to be involved in different vesicle trafficking processes. In earlier work we identified Rab11a to be present in GLUT4-containing vesicles after insulin stimulation and showed its involvement in insulin-dependent glucose uptake. However, it remained elusive if Rab11a is directly activated by the insulin signalling cascade and at which step a potential activation occurs. To examine the GTP-loading of Rab11a, we introduced a biotinylated GTP-analog into H9c2-hIR cells, transiently overexpressing HA-tagged Rab11a, and measured its binding to the GTPase after insulin stimulation. We observed that Rab11a is transiently GTP-loaded after insulin stimulation with a 2.3 (+/-0.3) fold activation (n=5), reaching its maximum after 4 min and declining back to basal after additional 2 min. The activation of Rab11a is phosphatidylinositol 3-kinase (PI3-kinase) dependent and downstream of Akt, as shown by in vitro knockdown of this kinase. These data show that Rab11a is directly activated by insulin and represents an element of the GLUT4 trafficking machinery.


Cell Signal


Cellular signalling


Schwenk RW,Eckel J




Has Abstract


2007-04-01 00:00:00














  • The upstream stimulatory factor USF1 is regulated by protein kinase CK2 phosphorylation.

    abstract::The upstream stimulatory factors 1 (USF1) and 2 (USF2) are transcription factors which bind to E-box motifs of various promoters regulating a variety of different cellular processes. Only little is known about the regulation of USFs. Here, we identified protein kinase CK2 as an enzyme that phosphorylates USF1 but not ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Lupp S,Götz C,Khadouma S,Horbach T,Dimova EY,Bohrer AM,Kietzmann T,Montenarh M

    更新日期:2014-12-01 00:00:00

  • Downregulation of stathmin expression is mediated directly by Egr1 and associated with p53 activity in lung cancer cell line A549.

    abstract::Stathmin is overexpressed in a variety of assessed human malignancies and is correlated with tumor progression and poor prognosis. Downregulation of its expression will contribute to optimize therapeutic outcomes in the treatment of various malignancies. However, the mechanisms of stathmin gene overexpression are not ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Fang L,Min L,Lin Y,Ping G,Rui W,Ying Z,Xi W,Ting H,Li L,Ke D,Jihong R,Huizhong Z

    更新日期:2010-01-01 00:00:00

  • Functional decreases in P2X7 receptors are associated with retinoic acid-induced neuronal differentiation of Neuro-2a neuroblastoma cells.

    abstract::Neuro-2a (N2a) cells are derived from spontaneous neuroblastoma of mouse and capable to differentiate into neuronal-like cells. Recently, P2X7 receptor has been shown to sustain growth of human neuroblastoma cells but its role during neuronal differentiation remains unexamined.We characterized the role of P2X7 recepto...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wu PY,Lin YC,Chang CL,Lu HT,Chin CH,Hsu TT,Chu D,Sun SH

    更新日期:2009-06-01 00:00:00

  • Mutational analysis reveals separable DNA binding and trans-activation of Drosophila STAT92E.

    abstract::In the canonical model of JAK/STAT signalling STAT transcription factors are activated by JAK mediated tyrosine phosphorylation following pathway stimulation by external cytokines. Activated STAT molecules then homo- or heterodimerise before translocating to the nucleus where they bind to DNA sequences within the prom...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Karsten P,Plischke I,Perrimon N,Zeidler MP

    更新日期:2006-06-01 00:00:00

  • Protein kinase C-β mediates neuronal activation of Na(+)/H(+) exchanger-1 during glutamate excitotoxicity.

    abstract::Na(+)/H(+) exchanger-1 (NHE-1) activity is known to play a critical role in the neuronal injury caused by glutamate. However, the underlying mechanism is not clear. This study shows that NHE-1 activation and its phosphorylation during glutamate exposure were attenuated by the inhibition of protein kinase C (PKC)-βI an...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Lee BK,Yoon JS,Lee MG,Jung YS

    更新日期:2014-04-01 00:00:00

  • Factors affecting prostacyclin receptor agonist efficacy in different cell types.

    abstract::Octimibate and related nonprostanoid prostacyclin mimetics are partial agonists displaying highly tissue-specific responses. Octimibate demonstrated considerably greater efficacy for stimulation of adenylyl cyclase activity in Chinese hamster ovary cells transiently expressing mouse prostacyclin receptors (mIP-CHO cel...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Kam Y,Chow KB,Wise H

    更新日期:2001-11-01 00:00:00

  • Promyogenic function of Integrin/FAK signaling is mediated by Cdo, Cdc42 and MyoD.

    abstract::The Integrin-mediated cell adhesion to the extracellular matrix is implicated in the control of proliferation, survival, migration and differentiation of myoblasts. Focal adhesion kinase (FAK) mediates signals from Integrins and plays an essential role in myotube formation. Cdo forms a multiprotein complex that includ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Han JW,Lee HJ,Bae GU,Kang JS

    更新日期:2011-07-01 00:00:00

  • Role of glycosyl-phosphatidylinositol hydrolysis as a mitogenic signal for epidermal growth factor.

    abstract::We have investigated the role of the hydrolysis of glycosyl-phosphatidylinositol (GPI) as one of the signalling pathways elicited after interaction of epidermal growth factor (EGF) with its specific plasma membrane receptor (EGFR). Endogenous GPI was characterized in both NIH 3T3 mouse fibroblast cells and in EGFR-tra...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Clemente R,Jones DR,Ochoa P,Romero G,Mato JM,Varela-Nieto I

    更新日期:1995-05-01 00:00:00

  • Activated EGF receptor may balance ERK-inhibitory network signalling pathways.

    abstract::In the COS-7 cell signalling network high levels of cAMP produced, for example, by co-stimulation of beta2-adrenergic receptor (beta2-AR) and bradykinin B2 receptor (BKR) may affect epidermal growth factor receptor (EGFR)-mediated activation of extracellular signal-stimulated kinase (ERK). In contrast, co-stimulation ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Hanke S,Valkova C,Stirnweiss J,Drube S,Liebmann C

    更新日期:2006-07-01 00:00:00

  • Inter- and intra-cellular mechanism of NF-kB-dependent survival advantage and clonal expansion of radio-resistant cancer cells.

    abstract::Understanding the underlying mechanism by which cancer cells acquire resistance to radiation and favorably selected for its clonal expansion will provide molecular insight into tumor recurrence at the treatment site. In the present study, we investigated the molecular mechanisms prompted in MCF-7 breast cancer cells i...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Yu H,Aravindan N,Xu J,Natarajan M

    更新日期:2017-02-01 00:00:00

  • Glycogen synthase kinase-3 regulates microglial migration, inflammation, and inflammation-induced neurotoxicity.

    abstract::Microglia play a prominent role in the brain's inflammatory response to injury or infection by migrating to affected locations, secreting inflammatory molecules, and phagocytosing damaged tissue. However, because severe or chronic neuroinflammation exacerbates many neurological conditions, controlling microglia action...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Yuskaitis CJ,Jope RS

    更新日期:2009-02-01 00:00:00

  • Molecular interactions of SHP1 and SHP2 in IL-3-signalling.

    abstract::SHP1 and SHP2 tyrosine phosphatases have both been implicated in signalling pathways downstream of the interleukin-3 (IL-3) receptor. We have investigated the co-association of SHP1 and SHP2 with tyrosine-phosphorylated proteins in IL-3-dependent BaF/3 cells. We demonstrate that both SHP1 and SHP2 associate with Aic2A...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wheadon H,Paling NR,Welham MJ

    更新日期:2002-03-01 00:00:00

  • Apelin signalling: a promising pathway from cloning to pharmacology.

    abstract::The discovery of new signalling pathways is always followed by the development of pharmacological agents as drugs that can be used in the treatment of diseases resulting from a dysfunction of the signalling pathway in question. Apelin signalling plays a role in the central and peripheral regulation of the cardiovascul...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Masri B,Knibiehler B,Audigier Y

    更新日期:2005-04-01 00:00:00

  • Suppression of GATA-3 increases adipogenesis, reduces inflammation and improves insulin sensitivity in 3T3L-1 preadipocytes.

    abstract::Impaired adipogenesis plays an important role in the development of obesity-associated insulin resistance and type 2 diabetes. Adipose tissue inflammation is a crucial mediator of this process. GATA-3 plays important roles in adipogenesis and inflammation. The aim of this study is to investigate the impact of GATA-3 s...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Al-Mansoori L,Al-Jaber H,Madani AY,Mazloum NA,Agouni A,Ramanjaneya M,Abou-Samra AB,Elrayess MA

    更新日期:2020-11-01 00:00:00

  • Proteoglycan signaling co-receptors: roles in cell adhesion, migration and invasion.

    abstract::Signaling co-receptors are diverse, multifunctional components of most major signaling pathways, with roles in mediating and regulating signaling in both physiological and pathophysiological circumstances. Many of these signaling co-receptors, including CD44, glypicans, neuropilins, syndecans and TssRIII/betaglycan ar...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Mythreye K,Blobe GC

    更新日期:2009-11-01 00:00:00

  • Capsaicin induces cytotoxicity in pancreatic neuroendocrine tumor cells via mitochondrial action.

    abstract::Capsaicin (CAP), the pungent ingredient of chili peppers, inhibits growth of various solid cancers via TRPV1 as well as TRPV1-independent mechanisms. Recently, we showed that TRPV1 regulates intracellular calcium level and chromogranin A secretion in pancreatic neuroendocrine tumor (NET) cells. In the present study, w...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Skrzypski M,Sassek M,Abdelmessih S,Mergler S,Grötzinger C,Metzke D,Wojciechowicz T,Nowak KW,Strowski MZ

    更新日期:2014-01-01 00:00:00

  • Phorbol ester effects on hormonal responses in freshly isolated short-term incubated and cultured hepatocytes.

    abstract::Beta-adrenergic, alpha-1-adrenergic and glucagon stimulation of glucose release were compared between hepatocytes which were freshly isolated, incubated for 3 h in suspension or cultivated for 4 or 24 h in plastic culture flasks in the presence and absence of the protein kinase C activator 12-O-tetradecanoylphorbol-13...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Hermsdorf T,Dettmer D

    更新日期:1992-11-01 00:00:00

  • Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signalling.

    abstract::Disabled-2 (DAB2) is an adapter protein that plays a key role in cell proliferation and differentiation. We reported here that DAB2 is expressed in various regions of rat central nervous system and is most abundant in the olfactory bulb. The up-regulation of DAB2 upon 5,7-dihydroxytryptamine-induced spinal cord lesion...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Huang CH,Cheng JC,Chen JC,Tseng CP

    更新日期:2007-06-01 00:00:00

  • Curcumin represses mTORC1 signaling in Caco-2 cells by a two-sided mechanism involving the loss of IRS-1 and activation of AMPK.

    abstract::The mechanistic target of rapamycin complex 1 (mTORC1) is a central modulator of inflammation and tumorigenesis in the gastrointestinal tract. Growth factors upregulate mTORC1 via the PI3K/AKT and/or Ras/MAPK signal pathways. Curcumin (CUR), a polyphenol found in turmeric roots (Curcuma longa) can repress mTORC1 kinas...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Kaur H,Moreau R

    更新日期:2021-02-01 00:00:00

  • Platelet-derived growth factor (PDGF) induces pulmonary vascular remodeling through 15-LO/15-HETE pathway under hypoxic condition.

    abstract::15-lipoxygenase (15-LO) is known to play an important role in chronic pulmonary hypertension. Accumulating evidence for its down-stream participants in the vasoconstriction and remodeling processes of pulmonary arteries, while how hypoxia regulates 15-LO/15-hydroxyeicosatetraenoic acid (15-HETE) to mediate hypoxic pul...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Zhang L,Ma J,Shen T,Wang S,Ma C,Liu Y,Ran Y,Wang L,Liu L,Zhu D

    更新日期:2012-10-01 00:00:00

  • TMPRSS4 upregulates uPA gene expression through JNK signaling activation to induce cancer cell invasion.

    abstract::TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed in pancreatic, thyroid, colon, and other cancer tissues. Previously, we demonstrated that TMPRSS4 mediates tumor cell invasion, migration, and metastasis. However, the mechanisms by which TMPRSS4 contributes to invasion are not fully und...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Min HJ,Lee Y,Zhao XF,Park YK,Lee MK,Lee JW,Kim S

    更新日期:2014-02-01 00:00:00

  • Protein kinase A downregulates the phosphorylation of p47 phox in human neutrophils: a possible pathway for inhibition of the respiratory burst.

    abstract::Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A(PKA) is considered to be a physiologic modulator of superoxide generation by stimulated neutrophils. Mechanisms of the inhibitory action of PKA are poorly understood. In this study, we investigated effects of cAMP-elevating agents on the phosphorylation ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Bengis-Garber C,Gruener N

    更新日期:1996-06-01 00:00:00

  • HO-1 and JAK-2/STAT-1 signals are involved in preferential inhibition of iNOS over COX-2 gene expression by newly synthesized tetrahydroisoquinoline alkaloid, CKD712, in cells activated with lipopolysacchride.

    abstract::We found that CKD712, an S enantiomer of YS49, strongly inhibited inducible nitric oxide synthase (iNOS) and NO induction but showed a weak inhibitory effect on cyclooxygenase-2 (COX-2) and PGE(2) induction in LPS-stimulated RAW 264.7 cells. We, therefore, investigated the molecular mechanism(s) responsible for this b...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Tsoyi K,Kim HJ,Shin JS,Kim DH,Cho HJ,Lee SS,Ahn SK,Yun-Choi HS,Lee JH,Seo HG,Chang KC

    更新日期:2008-10-01 00:00:00

  • A soluble LAT deletion mutant inhibits T-cell activation: reduced recruitment of signalling molecules to glycolipid-enriched microdomains.

    abstract::The type III transmembrane adaptor protein linker for activation of T cells (LAT) is essential for membrane recruitment of signalling molecules following TCR activation. Here we show that although LAT deleted in the transmembrane domain is completely soluble, it can be tyrosine phosphorylated after anti-CD3 stimulatio...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Torgersen KM,Vaage JT,Rolstad B,Taskén K

    更新日期:2001-03-01 00:00:00

  • Major occurrence of the new alpha2beta1 isoform of NO-sensitive guanylyl cyclase in brain.

    abstract::NO-sensitive guanylyl cyclase (GC) acts as the effector molecule for NO and therefore plays a key role in the NO/cGMP signalling cascade. Besides the long known GC isoform (alpha(1)beta(1)), another heterodimer (alpha(2)beta(1)) has recently been identified to be associated with PSD-95 in brain.Here, we report on the ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Mergia E,Russwurm M,Zoidl G,Koesling D

    更新日期:2003-02-01 00:00:00

  • Structure and function of phosphatidylinositol-3,4 kinase.

    abstract::Activation of phosphatidylinositol (PI)-kinase is involved in the regulation of a wide array of cellular activities. The enzyme exists as a dimer, consisting of a catalytic and a regulatory subunit. Five isoforms of the regulatory subunit have been identified and classified into three groups comprising respectively 85...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Funaki M,Katagiri H,Inukai K,Kikuchi M,Asano T

    更新日期:2000-03-01 00:00:00

  • Activation of phosphoinositide 3-kinase/PKB pathway by CB(1) and CB(2) cannabinoid receptors expressed in prostate PC-3 cells. Involvement in Raf-1 stimulation and NGF induction.

    abstract::Cannabinoids exert a variety of physiological and pharmacological responses in humans through interaction with specific cannabinoid receptors. Cannabinoid receptors described to date belong to the seven-transmembrane-domain receptor superfamily and are coupled through the inhibitory G(i) protein to adenylyl cyclase in...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Sánchez MG,Ruiz-Llorente L,Sánchez AM,Díaz-Laviada I

    更新日期:2003-09-01 00:00:00

  • Overexpression of dJmj differentially affects intestinal stem cells and differentiated enterocytes.

    abstract::Jumonji (Jmj)/Jarid2 is a DNA-binding transcriptional repressor mediated via histone methylation. Nevertheless, the well-known function of Jmj is as a scaffold for the recruitment of various complexes including Polycomb repressive complex 2 (PRC2), and required for mouse embryonic stem cell development. However, PRC2 ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Suong DNA,Shimaji K,Pyo JH,Park JS,Yoshida H,Yoo MA,Yamaguchi M

    更新日期:2018-01-01 00:00:00

  • Transglutaminase 2 as a novel activator of LRP6/β-catenin signaling.

    abstract::The β-catenin signaling axis is critical for normal embryonic development and tissue homeostasis in adults. We have previously shown that extracellular enzyme transglutaminase 2 (TG2) activates β-catenin signaling in vascular smooth muscle cells (VSMCs). In this study, we provide several lines of evidence that TG2 fun...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Deasey S,Nurminsky D,Shanmugasundaram S,Lima F,Nurminskaya M

    更新日期:2013-12-01 00:00:00

  • RhoA/ROCK I signalling downstream of the P2Y13 ADP-receptor controls HDL endocytosis in human hepatocytes.

    abstract::Cell surface receptors for high-density lipoprotein (HDL) on hepatocytes are major partners in the regulation of cholesterol homeostasis. We have previously demonstrated on human hepatocytes that apolipoprotein A-I binding to an ectopic F(1)-ATPase stimulates the production of extracellular ADP that activates a P2Y(13...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Malaval C,Laffargue M,Barbaras R,Rolland C,Peres C,Champagne E,Perret B,Tercé F,Collet X,Martinez LO

    更新日期:2009-01-01 00:00:00