Abstract:
OBJECTIVE:The initial objective of this renal cancer study was to identify gene sets in clear cell renal cell carcinoma (ccRCC) to support grading of ccRCC histopathology. MATERIALS AND METHODS:Preselected ccRCC tumor tissues of grade 1 (G1, n = 14) and grade 3 (G3, n = 15) as well es 14 normal kidney tissues thereof were subjected to microarray expression analysis using Human Genome U133 Plus 2.0 Array. Event ratio scoring, hierarchical clustering and principal component analysis were used to determine gene sets that distinguish expression profiles from normal kidney tissue, G1 and G3 tumor tissues. RESULTS:An initial set of 73 genes provided seven gene subclusters (SC01 to SC07) that distinguish RNA expression profiles from G1, G3 tumor and normal kidney tissues. A ranked list of 24 genes was determined that separated G1 from G3 tumors in high concordance with histopathological grading confirmed by immunohistochemical analysis of ceruloplasmin protein expression. CONCLUSION:A final set of 24 genes has been determined awaiting further validation on the RNA as well as on the protein level by studying an additional cohort of ccRCC patients. A reliable separation of G1 and G3 tumor grades will be instrumental to foster and direct the administration of upcoming targeted therapeutics of ccRCC tumors in a more predictive and reliable manner.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Maruschke M,Koczan D,Reuter D,Ziems B,Nizze H,Hakenberg OW,Thiesen HJdoi
10.1159/000328196subject
Has Abstractpub_date
2011-01-01 00:00:00pages
205-17issue
2eissn
0042-1138issn
1423-0399pii
000328196journal_volume
87pub_type
杂志文章abstract:OBJECTIVES:To cultivate primary human bladder smooth muscle cells (hBSMCs) in vitro and to study the influences of different sustained hydrostatic pressures upon hBSMCs in terms of cell proliferation and expression of gap-junction connexin 43 (Cx43). METHODS:hBSMCs were exposed to 0, 20, 40, 60, 80 and 100 cm H(2)O su...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000338924
更新日期:2013-01-01 00:00:00
abstract::We examined Southern blot analysis of genomic DNAs from 70 patients with sporadic renal cell carcinoma, using the human L-myc oncogene fragment as a hybridization probe. Our purpose was to study the relationship between the restriction fragment length polymorphism (RFLP) of the L-myc and the frequencies of metastasis....
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282261
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Limited data are available for the use of agents in metastatic castration-resistant prostate cancer (mCRPC) beyond the third-line. We provide data during treatment with cabazitaxel (CAB), helping to improve the informed-consent process. PATIENTS AND METHODS:We retrospectively reviewed patients treated with ...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究,评审
doi:10.1159/000477943
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION AND AIM:Surgical vasectomy remains the gold standard for fertility control in men. Endoluminal occlusion of the seminal ducts, thus avoiding an external incision, may become an appealing alternative to this approach. As our group has shown, nontraumatic endoscopic inspection of the seminal ducts is feasibl...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000478790
更新日期:2017-01-01 00:00:00
abstract::Intrarenal blood flow distribution was measured in normal and hypoperfused dog kidneys by xenon-133 washout measurement in one kidney vary slightly in contrast to those done in different kidneys. In normal kidneys the ratio of compartment I (CI) flow to whole renal flow -- i.e., the CI fraction--as measured by the xen...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280818
更新日期:1982-01-01 00:00:00
abstract::High concentrations of phosphate, oxalate, and calcium ions in urine may cause formation of mineral deposits, i.e., urolithiasis. This is prevented in healthy individuals by substances present in trace quantities. However, there is no recognizable difference between normals and stone formers in urinary substance conte...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281098
更新日期:1985-01-01 00:00:00
abstract::The ability of three compounds, all similar in chemical structure to citric acid, to decrease calcium crystalluria has been measured. The measurements were made in normal human urine at 37 degrees C and compared with the crystal-decreasing power of citric acid when measured in the same way and in the same urine sample...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282875
更新日期:1996-01-01 00:00:00
abstract::We report a case of a 75-year-old female with pheochromocytoma of the bladder. Clinical evaluation included ultrasonography, intravenous pyelography, CT scan, I-MIBG scintiscan. A transurethral resection was performed for a exophytic tumor of 2 cm diameter. The histological result indicated the diagnosis of bladder ph...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000064868
更新日期:2000-01-01 00:00:00
abstract::The rate of recurrence after internal urethrotomy a.m. Sachse was studied in a prospective, consecutive, randomized series of 43 patients using short-term (1 day) and long-term (14 days) postoperative catheter treatment. No significant difference was found between the two groups according to voiding interview, flowmet...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000280991
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:This study aims to examine the relationship between chronic prostatic inflammation and prostatic calculi, and clinical parameters of benign prostatic hyperplasia (BPH). MATERIALS AND METHODS:This study was based on 225 patients who underwent transurethral resection of the prostate for BPH. Chronic inflammati...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000342643
更新日期:2013-01-01 00:00:00
abstract::The present study was undertaken to analyze the changes in neuroendocrine cells of the human prostate induced by neoplasms and the effect of hormonal treatment. Samples of human prostate (n = 47) were obtained during surgery or removal of organs for transplantation. The cases analyzed represent normal prostates (n = 4...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000283051
更新日期:1997-01-01 00:00:00
abstract::The prevalence, pathogenesis and clinical aspects of bacterial infections of the female urinary tract are described and the efficacy of therapies used at present is discussed. Based on a retrospective compilation of data on 142 female patients with urinary tract infections, the pathogen spectrum is defined and the the...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281989
更新日期:1987-01-01 00:00:00
abstract::The influence of unilateral ureteral ligation in the rat nephron has been morphometrically analysed. The glomeruli show no significant differences compared with the control groups. The lumen of the tubulus rectus I increases significantly, but irregularly, after the 6th day of ureter ligation. The brush border is most...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280329
更新日期:1980-01-01 00:00:00
abstract::Transrectal ultrasonography was performed in 46 patients with hemospermia. Abnormal findings in the prostate and seminal vesicles, including prostatic stones, benign prostatic hyperplasia, prostatitis, and dilatation and calculi of the seminal vesicles, were observed in 34 patients (73.9%). However, no malignant lesio...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282655
更新日期:1994-01-01 00:00:00
abstract::Urethral diverticula are pouches opening into the urethral canal that may be either acquired or congenital. Diverticula of the female urethra are fairly common. However, diverticula of the male urethra are relatively uncommon abnormalities. Clinical manifestation is frequently described with irritative and/or obstruct...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000346321
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Open radical cystectomy (ORC) is currently the standard treatment for muscle-invasive bladder cancer (MIBC) without metastasis, while many patients with MIBC are not always appropriate candidates due to multiple comorbidities. To evaluate the bladder-preservation strategy, we compared the results with thos...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000477912
更新日期:2017-01-01 00:00:00
abstract::The term infection stones refers to calculi that occur following urinary tract infections (UTIs) caused by urease-producing gram-negative organisms. They consist of magnesium ammonium phosphate, carbonate apatite and monoammonium urate. Alkaline urine is most favorable to their formation. Urinary tract obstruction, ne...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000104439
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Wnt-1 is capable of inducing metanephric mesenchyme to undergo tubulogenesis. A relationship between the degree of cystogenesis and reduced E-cadherin (E-cad) expression was described. Syndecan-1 (Sdc-1) has a critical role in kidney development. MATERIALS AND METHODS:Ten multicystic dysplastic kidneys (M...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000296285
更新日期:2010-01-01 00:00:00
abstract::Experience with chemodissolution of uric acid stones in 30 patients is presented. Chemodissolution was achieved either with infusion of 0.16 M i.v. lactate or oral sodium bicarbonate, in addition to liberal fluid intake and allopurinol wherever indicated. In some cases direct chemodissolution by in situ irrigation wit...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282302
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:To investigate the effect of hydrodynamic pressure mimicking vesicoureteral reflux on renal tubular epithelial cells in vitro, we constructed an intermittent pressure-loading (IPL) model of Madin-Darby canine kidney (MDCK) cells. MATERIALS AND METHODS:Three grades of pressure were loaded onto the MDCK cells...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000087170
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:To assess the influence of music therapy on perceived anxiety and pain during outpatient urodynamic study (UDS) in a prospective, randomized fashion. METHODS:Between January and December 2018, a total of 70 patients were randomized to either have music therapy (study) or not have (control) in a 1:1 ratio....
journal_title:Urologia internationalis
pub_type: 杂志文章,随机对照试验
doi:10.1159/000502298
更新日期:2019-01-01 00:00:00
abstract::Patients with prostatic carcinoma were randomised to treatment with surgical castration (n = 47) or oral oestrogen therapy (n = 53). The oestrogen-treated patients received oral ethinyl oestradiol daily, 1 mg for the first 2 weeks followed by 150 micrograms. In addition, oestrogen was given intramuscularly as polyoest...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282639
更新日期:1994-01-01 00:00:00
abstract::A 63-year-old man underwent inguinal orchiectomy for painless enlargement of the left testis. The diagnosis of malignant gonadal stromal tumor was made on the basis of morphology and immunohistochemical studies. At computed tomography there was bulky retroperitoneal metastasis. The patient then underwent retroperitone...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000283061
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to describe a novel negative-pressure laser lithotripsy device to overcome the deficiencies of the conventional procedure. PATIENTS AND METHODS:Between August 2018 and March 2019, 78 patients with a single ureteral stone underwent retrograde ureteroscopy with a Wolf 8F/9.8F rigid u...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000507266
更新日期:2020-01-01 00:00:00
abstract::During a 2-year period, 37 patients with symptomatic metastatic prostatic cancer were included in a prospective randomized phase-III trial. Nineteen patients were randomized to subcapsular orchiectomy, and 18 to cyproterone acetate (CPA) treatment with a dose of 50 mg b.i.d. The median age of the patients was 74 years...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282125
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:The study aimed to present our experience of surgical treatment for urorectal fistulas (URF) that develop after cancer surgery. MATERIALS AND METHODS:Fourteen patients with URF who were treated at our institution from 2005 through 2015 were retrospectively analyzed. Among these, 7 patients had previous su...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000457835
更新日期:2017-01-01 00:00:00
abstract::Emphysematous cystitis is a rare disorder most commonly seen in patients with urinary tract infection and diabetes mellitus. We present a nondiabetic case of a 46-year-old woman with this entity following brain contusion. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000030520
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:To compare the safety and efficacy of standard-tract combined with mini-tract to single standard-tract in percutaneous nephrolithotomy (PCNL) for renal staghorn calculi. METHODS:The records of 216 patients with staghorn calculi (110 (50.9%) had complete and 106 (49.1%) had partial) who received PCNL were revie...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000354427
更新日期:2014-01-01 00:00:00
abstract::Human factors is a definition that includes the science of understanding the properties of human capability, the application of this understanding to the design and development of systems and services, the art of ensuring their successful applications to a program. The field of human factors traces its origins to the ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000241662
更新日期:2009-01-01 00:00:00
abstract::The functional activity of the sphincter muscle of the urethra is known to be controlled largely by the hypogastric and pudendal nerves. It remains unknown, however, whether innervation of the muscle by these peripheral nerves is ipsi- or bilateral. In an attempt to answer this question urethral closure pressure was d...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281328
更新日期:1988-01-01 00:00:00