Abstract:
OBJECTIVES:To cultivate primary human bladder smooth muscle cells (hBSMCs) in vitro and to study the influences of different sustained hydrostatic pressures upon hBSMCs in terms of cell proliferation and expression of gap-junction connexin 43 (Cx43). METHODS:hBSMCs were exposed to 0, 20, 40, 60, 80 and 100 cm H(2)O sustained hydrostatic pressures for 6, 12, 24 and 72 h, respectively. hBSMC proliferation was quantified with Cell Counting Kit-8 (CCK-8) afterwards. Immunofluorescence staining and Western blotting were employed to determine hBSMC Cx43 expression. RESULTS:hBSMC proliferation increased with time (6-72 h) without significant differences between pressure levels at each time point. Expression of Cx43 on Western blotting and immunofluorescence staining was similar under varied pressures for 6 and 12 h while seeing significant decreases under hydrostatic pressures >60 cm H(2)O for 24 h or pressures >40 cm H(2)O for 72 h. CONCLUSIONS:Different sustained hydrostatic pressures (<100 cm H(2)O) bear no significant effects on proliferation of hBSMCs. Interestingly, hydrostatic pressures above the physiological level seem to cause the gap junctions of hBSMCs to decrease significantly and affect the cellular functions in the cultivation group over a longer period of time.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Wang Y,Wang K,Li H,Chen L,Xu F,Wu Tdoi
10.1159/000338924subject
Has Abstractpub_date
2013-01-01 00:00:00pages
75-82issue
1eissn
0042-1138issn
1423-0399pii
000338924journal_volume
90pub_type
杂志文章abstract:INTRODUCTION:Mini percutaneous nephrolithotomy (mini-PCNL) and retrograde intrarenal surgery (RIRS) are well-established techniques with little morbidity. The combined use of standard PCNL and the mini-PCNL or the RIRS technique was evaluated and compared to investigate their own role in the management of staghorn calc...
journal_title:Urologia internationalis
pub_type: 杂志文章,随机对照试验
doi:10.1159/000360708
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:To determine association between urine pH and OAB symptoms and to determine if urinary alkalization improves overactive bladder (OAB) symptoms. METHODS:659 patients with OAB were enrolled in this study between June 2012 and May 2014. 329 patients (group 1) were included in the final analysis. 201 adults wer...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000375322
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To assess clinical outcomes and reimbursement costs of open and robotic-assisted radical prostatectomies in Germany. METHODS:Perioperative data of 499 open (2003-2006) and 932 (2008-2010) robotic-assisted radical prostatectomies as well as longitudinal reimbursement costs of an anonymized health insurance rese...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000431104
更新日期:2016-01-01 00:00:00
abstract::Nutrition plays a major role in the pathogenesis of the most widespread forms of nephrolithiasis, i.e. calcium (calcium oxalate and phosphate) and uric acid stone disease. For this reason, dietary measures are the first level of intervention in primary prevention, as well as in secondary prevention of recurrences. An ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000076588
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:In order to accurately assess the extent of chronic pelvic pain syndrome (CPPS) and to objectively measure symptoms for natural history studies and to assess the outcome parameters for clinical trials, the National Institutes of Health (NIH) Chronic Prostatitis Collaborative Research Network developed and ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000094814
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:This article systematically analyses comparative studies to evaluate the efficacy and safety of tubeless percutaneous nephrolithotomy (PCNL) versus standard PCNL. METHODS:The Medline, EMBASE, PsycINFO, Cochrane and DARE databases were searched from 1997 to February 2011. Comparative studies evaluating outcom...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000336145
更新日期:2012-01-01 00:00:00
abstract::Technetium-labeled red blood cell scintigraphy using a bolus injection was used to obtain a noninvasive quantitative measurement of renal blood flow. The method used is an adaptation of the one used for calculating cerebral blood flow which involves deconvolution of the first pass of the isotope through the kidneys. T...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281863
更新日期:1987-01-01 00:00:00
abstract::We present a 17-year-old patient with a right testicular dislocation. In this case, dislocated testis had been considered as hematoma and therefore remained untreated for 4 months. The patient underwent right orchiopexy 4 months after the injury. Intraoperative testicular biopsy revealed impaired spermatogenesis and e...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282830
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To investigate whether or not the age of spontaneous resolution of monosymptomatic nocturnal enuresis (MNE) was familial. PATIENTS AND METHODS:A questionnaire was administered to more than 1,500 people, and 100 appropriate participants were identified from four referral hospitals. We included the participants ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000370166
更新日期:2015-01-01 00:00:00
abstract::During a 2-year period, 37 patients with symptomatic metastatic prostatic cancer were included in a prospective randomized phase-III trial. Nineteen patients were randomized to subcapsular orchiectomy, and 18 to cyproterone acetate (CPA) treatment with a dose of 50 mg b.i.d. The median age of the patients was 74 years...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282125
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:The role of positron emission tomography (PET) with 2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG) is currently under evaluation in urologic oncology. The aim of the present study was to investigate the use of [18F]FDG positron emission tomography ([18F]FDG-PET) in the detection and treatment control of malign...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000048442
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:The principle of treating male infertility in those with chromosomal aberrations should change according to progress in treatment modalities. METHODS:In this study, ten patients with Robertsonian translocation were assessed for their cytogenetic and clinical characteristics to establish guidelines for treat...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000066122
更新日期:2002-01-01 00:00:00
abstract::Urethral diverticula are pouches opening into the urethral canal that may be either acquired or congenital. Diverticula of the female urethra are fairly common. However, diverticula of the male urethra are relatively uncommon abnormalities. Clinical manifestation is frequently described with irritative and/or obstruct...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000346321
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To assess the feasibility and performance of radical cystectomy with urinary diversion using exclusively regional anesthesia (i.e. combined spinal thoracic epidural anesthesia, CSTEA), avoiding the adverse effects of general anesthesia. MATERIALS AND METHODS:In our hospital, radical cystectomy with extended ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000348542
更新日期:2013-01-01 00:00:00
abstract::To clarify the relative importance of clinicopathological factors affecting survival in patients with renal cell carcinoma, univariate and multivariate analyses by Cox's proportional hazards model were performed for 121 patients undergoing nephrectomy between 1980 and 1991. The 5-year survival rate was 67% for all 121...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282439
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVES:This open label, phase I clinical trial (NCT02945462) using 2 consecutive intracavernous autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) for the first time in the treatment of diabetic patients with erectile dysfunction (ED). The primary outcome is to assess the safety and tolerability of int...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000492120
更新日期:2018-01-01 00:00:00
abstract::The prevalence of Chlamydia trachomatis and other microbes was studied in 94 semen samples from asymptomatic infertile males. Simultaneously, we sought evidence for inflammation of the genital tract by determining the polymorphonuclear granulocyte (PMN)-elastase concentration in the seminal plasma. The C. trachomatis ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282675
更新日期:1994-01-01 00:00:00
abstract::The aim of the present paper was to review findings from the most relevant studies and to evaluate the value of current chemotherapy and surgery in advanced unresectable and metastatic bladder cancer. Studies were identified by searching the MEDLINE® and PubMed® databases up to 2011 using both medical subject heading ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000335556
更新日期:2012-01-01 00:00:00
abstract::Controversies abound in relation to the treatment of every stage of prostate cancer and its natural history. We critically evaluate the controversies and information that exist. An ongoing autopsy project at Wayne State University aims to: define the prevalence of incidental cancer in subjects between 20 and 70 years ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000282860
更新日期:1996-01-01 00:00:00
abstract:INTRODUCTION:The treatment of large complete staghorn calculi requires a sandwich combination of percutaneous nephrolithotomy (PCNL) and shockwave lithotripsy (SWL) or sometimes open surgery. Many urologists hesitate to place more than 2-3 tracts during PCNL because of the belief that this may increase complications. W...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000089168
更新日期:2005-01-01 00:00:00
abstract::119 patients with stage-IV renal cell carcinoma were treated using immunotherapy with autologous tumor vaccine. The immunization was carried out at monthly intervals, the patients were restaged every 3 months using X-ray, ultrasound scanning or computed tomography as well as bone scintigraphy in the follow-up. The pat...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000281439
更新日期:1989-01-01 00:00:00
abstract::Testicular microlithiasis associated with seminoma was found in a 32-year-old infertile man. Gray-scale and color Doppler ultrasound findings are discussed and a brief review of the literature is presented. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282928
更新日期:1996-01-01 00:00:00
abstract::We report the case of a woman who presented with obstruction and urosepsis of her left kidney secondary to small cystine calculi. The calculi could not be seen on initial plain abdominal X-ray. However, following percutaneous nephrostogram the calculi became more radio-dense and visible on later X-rays. Experimental e...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000030375
更新日期:1999-01-01 00:00:00
abstract:INTRODUCTION:We investigated the status of erectile function and urinary continence after radical prostatectomy to investigate a possible relation between them and then determined whether the status of postoperative urinary continence affected erectile function. PATIENTS AND METHODS:Seventy-six patients who had no sym...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000078801
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Male infertility caused by azoospermia due to non-reconstructable obstruction or non-obstructive azoospermia can be treated by microsurgical epididymal aspiration (MESA) or testicular sperm extraction (TESE) followed by an intracytoplasmatic spermatozoa injection (ICSI). MATERIAL AND METHODS:From 9/93 to ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000068185
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVES:Endorectal coil MRI (endoMRI) of the prostate is useful to evaluate tumor localization. There is little evidence on patient characteristics affecting its diagnostic performance. We evaluate the influence of clinical and histological parameters on the accuracy of endoMRI. METHODS:Sixty-nine patients with pro...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000324098
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:Our aim was to determine the safety and feasibility of catheter removal on the first morning after transurethral prostatectomy. METHODS:Fifty-four patients who underwent transurethral prostatectomy were included in a prospective study. The decision to remove the catheters on the first morning after surgery w...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000048428
更新日期:2002-01-01 00:00:00
abstract::A partial urethral obstruction was induced in female rats. After 6 weeks, detrusor weight had increased eightfold and collagen concentration had decreased to about 40% compared to controls. Due to the weight gain, the total amount of detrusor collagen had increased more than threefold. Removal of the obstruction led t...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282230
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:No established technique for locating solitary carcinoma in situ (CIS) of the urinary bladder or CIS accompanying bladder cancer has been determined. Here we investigated whether the location of CIS of the urinary bladder can be macroscopically ascertained by instilling pirarubicin hydrochloride (THP) into...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000083555
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:The aim of our study was to determine the validity of the updated nomogram [Briganti et al.: Eur Urol 2012;61:480-487] as a prediction tool for pelvic lymph node invasion (LNI) in the current era by using a large multicentric population of men who underwent extended pelvic lymph node dissection (ePLND) at ...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000343993
更新日期:2013-01-01 00:00:00