Abstract：
:Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Čavić M,Grozdanović M,Bajić A,Srdić-Rajić T,Anđjus PR,Gavrović-Jankulović Mdoi
10.1016/j.phytochem.2011.12.014subject
Has Abstractpub_date
2012-05-01 00:00:00pages
46-52eissn
0031-9422issn
1873-3700pii
S0031-9422(11)00604-2journal_volume
77pub_type
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