Abstract：
:Prostaglandin E2 (PGE2) and proton are typical inflammatory mediators. They play a major role in pain processing and hypersensitivity through activating their cognate receptors expressed in terminals of nociceptive sensory neurons. However, it remains unclear whether there is an interaction between PGE2 receptors and proton-activated acid-sensing ion channels (ASICs). Herein, we show that PGE2 enhanced the functional activity of ASICs in rat dorsal root ganglion (DRG) neurons through EP1 and EP4 receptors. In the present study, PGE2 concentration-dependently increased ASIC currents in DRG neurons. It shifted the proton concentration-response curve upwards, without change in the apparent affinity of proton for ASICs. Moreover, PGE2 enhancement of ASIC currents was partially blocked by EP1 or EP4 receptor antagonist. PGE2 failed to enhance ASIC currents when simultaneous blockade of both EP1 and EP4 receptors. PGE2 enhancement was partially suppressed after inhibition of intracellular PKC or PKA signaling, and completely disappeared after concurrent blockade of both PKC and PKA signaling. PGE2 increased significantly the expression levels of p-PKCε and p-PKA in DRG cells. PGE2 also enhanced proton-evoked action potentials in rat DRG neurons. Finally, peripherally administration of PGE2 dose-dependently exacerbated acid-induced nocifensive behaviors in rats through EP1 and EP4 receptors. Our results indicate that PGE2 enhanced the electrophysiological activity of ASICs in DRG neurons and contributed to acidosis-evoked pain, which revealed a novel peripheral mechanism underlying PGE2 involvement in hyperalgesia by sensitizing ASICs in primary sensory neurons.
journal_name
Brain Resjournal_title
Brain researchauthors
Zhou YM,Wu L,Wei S,Jin Y,Liu TT,Qiu CY,Hu WPdoi
10.1016/j.brainres.2019.146442subject
Has Abstractpub_date
2019-12-01 00:00:00pages
146442eissn
0006-8993issn
1872-6240pii
S0006-8993(19)30496-2journal_volume
1724pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:As a means of probing the chemotransducer, impulse sequences from in vitro, superfused sinus nerve-carotid body preparations were analyzed for departures from randomness. Twelve of 17 spike trains showed interval histograms that did not differ significantly from exponential. Thus these sequences resembled a Poisson (r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90908-9
更新日期：1984-01-30 00:00:00
abstract：:The distributions of serotonin and catecholamines were examined within the central ganglia of juvenile Achatina through the histological localization of serotonin-like immunoreactivity and of glyoxylic acid-induced fluorescence. Somata containing these amines were widely distributed throughout all central ganglia exce...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90427-1
更新日期：1988-09-13 00:00:00
abstract：:Chicks of 1-8 days after hatching were submitted to unilateral visual deprivation and, in both optic lobes, muscarinic receptors, labeled with L-[3H]quinuclidinyl benzylate [( 3H]L-QNB) and acetylcholinesterase (AChE) were assayed. Between 1 and 6 days the number of [3H]L-QNB binding sites was lower in the contralater...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(84)90313-4
更新日期：1984-06-01 00:00:00
abstract：:The time course of the neurogenesis of cells showing glutamic acid decarboxylase (GAD) immunoreactivity has been analyzed in the dorsal hippocampus and area dentata of the mouse. The quantitative data indicate that gamma-aminobutyric acid (GABA)ergic cells are generated prenatally, and that their peaks of neurogenesis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(86)80012-9
更新日期：1986-11-01 00:00:00
abstract：:The auditory cortical projections to the medial geniculate body (MGB) were studied in the cat. Lesions carefully restricted to each one of the subdivisions of the auditory and peri-auditory cortex were made and degenerating fibres were mapped in the MGB. In other experiments L-[4-5-3-H]leucine was injected into the co...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90465-5
更新日期：1975-06-20 00:00:00
abstract：:The pathogenic mechanisms of gene-environment interactions determining variability of human neurodevelopmental disorders remain unclear. In the two consecutive papers, we used the neonatal Borna disease virus (BDV) infection rat model of neurodevelopmental damage to evaluate brain pathology, monoamine alterations, beh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02723-3
更新日期：2002-07-19 00:00:00
abstract：:This study was designed to assess effects of insulin-induced hypoglycemia on plasma and cerebrospinal fluid (CSF) levels of immunoreactive (ir) beta-endorphins, adrenocorticotropin (ACTH), cortisol, norepinephrine, insulin, and glucose in the conscious, overnight fasted dog. Dogs received either an intravenous infusio...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90475-1
更新日期：1988-08-23 00:00:00
abstract：:Acupuncture is widely used to relieve pain; however, the mechanism underlying electroacupuncture analgesia (EAA) during inflammatory pain is unclear. We investigated whether endogenous peripheral opioid receptors participated in EAA during hyperalgesia elicited by carrageenan-induced inflammation. Moreover, we investi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.08.014
更新日期：2010-10-08 00:00:00
abstract：:The role of GABA in NMDA-dependent long term depression (LTD) in the medial vestibular nuclei (MVN) was studied on rat brainstem slices. High frequency stimulation (HFS) of the primary vestibular afferents induces a long lasting reduction of the polysynaptic (N2) component of the field potentials recorded in the dorsa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00895-w
更新日期：1995-11-20 00:00:00
abstract：:Dopamine-beta-hydroxylase (DBH), the enzyme that converts dopamine to norepinephrine, has been localized in light and electron microscopic preparations of rat brain by an immunocytochemical method using a peroxidase--anti-peroxidase Fab complex. In light microscopic preparations, DBH-specific reaction product was obse...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90755-8
更新日期：1979-02-16 00:00:00
abstract：:Choline molecules are needed for the synthesis of acetylcholine and phospholipids in the mammalian brain. An enzymatic activity capable of forming lecithin (phosphatidylcholine) from the step-by-step methylation of phosphatidylethanolamine is identified in the bovine brain. This enzyme(s), phosphatidylethanolamine-N-m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90447-5
更新日期：1979-12-28 00:00:00
abstract：:ENT1 is an equilibrative nucleoside transporter that enables trans-membrane bi-directional diffusion of biologically active purines such as adenosine. In spinal cord dorsal horn and in sensory afferent neurons, adenosine acts as a neuromodulator with complex pro- and anti-nociceptive actions. Although uptake and relea...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.08.017
更新日期：2005-10-19 00:00:00
abstract：:Double-staining in either vibratome or paraffin sections using contrasting chromogens revealed an alpha-melanocyte-stimulating hormone (alpha-MSH)-containing cell group in the arcuate nucleus, a metorphamide-containing cell group in the paraventricular hypothalamus, and an extensive group of magnocellular perikarya in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90012-1
更新日期：1988-06-14 00:00:00
abstract：:In the rat, calbindin-like immunoreactivity was observed at both the light and electron microscopic levels within the chemoreceptor neurons of the vomeronasal organ (VNO) and both intragemmal cells and associated nerve fibers of the circumvallate taste buds. All VNO neurons were immunoreactive. Only a subset of intrag...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90493-s
更新日期：1992-02-14 00:00:00
abstract：:The role of N-methyl-D-aspartate (NMDA) receptors in specific D1 and D2 regulation of striatal and accumbens neurotensin (NT) systems was investigated. As demonstrated previously, stimulation of D1 receptors with multiple administrations of SKF 38393 significantly increased striatal and accumbens NT content to approxi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90663-t
更新日期：1992-02-07 00:00:00
abstract：:A feature of neurodegenerative disease is the accumulation of insoluble protein aggregates in the brain. In some conditions, including Amyotrophic Lateral Sclerosis and Frontotemporal lobar degeneration, the primary aggregating entities are RNA binding proteins. Through regulated prion-like assembly, RNA binding prote...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.05.052
更新日期：2014-10-10 00:00:00
abstract：:The effect of gamma-aminobutyric acid (GABA)receptor stimulation on norepinephrine (NE)-induced metabolism of polyphosphoinositides (PIPs) was studied in rat hippocampal slices. Inositol phosphates (IPs), PIPs, and phosphatidic acid were measured. NE induced formation of IPs and phosphatidic acid in a dose-dependent m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90701-3
更新日期：1987-05-12 00:00:00
abstract：:Even though animal and human studies show alterations in dopamine transporter (DAT) sites after alcohol withdrawal, the role of DAT in influencing either alcoholic or depressive behavior has not been examined extensively. Given that the Wistar-Kyoto (WKY) rat is a putative animal model of depressive behavior, the pres...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.12.009
更新日期：2006-02-16 00:00:00
abstract：:We studied the propagation of spike discharges and the changes in local cerebral blood flow (LCBF) by means of the electroencephalogram (EEG) and autoradiographs to elucidate the underlying mechanism and the propagation pathway in rats with limbic seizures. Seizures were induced by the unilateral microinjection of kai...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91923-2
更新日期：1994-01-21 00:00:00
abstract：:The object of this study was to quantify and compare the degree of control monkeys may assert over firing patterns of normal and epileptic neurons. Thirty-seven epileptic and 70 normal neurons were studied in detail. The operant task was for the monkey to generate specified consecutive interspike intervals (ISI). The ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90884-3
更新日期：1978-08-04 00:00:00
abstract：:The possibility of retinotopic organization of pathways of retinal fibers within the optic tract and the tectum of the frog was studied by selective labeling of the retinal fibers with horseradish peroxidase. Within the optic tract the pathways of the ventral, temporal and dorsal retinal fibers were ordered from the d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90097-4
更新日期：1981-02-09 00:00:00
abstract：:Parkinson's disease (PD) is caused by oxidative stress, and erythropoietin (EPO) reduces oxidative stress in the brain. However, EPO cannot be developed as a treatment for PD, because EPO does not cross the blood-brain barrier (BBB). A brain penetrating form of human EPO has been developed wherein EPO is fused to a ch...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.01.061
更新日期：2011-03-25 00:00:00
abstract：:Adaptive decision making in dynamic social settings requires frequent re-evaluation of choice outcomes and revision of strategies. This requires an array of multiple cognitive abilities, such as working memory and response inhibition. Thus, the disruption of such abilities in schizophrenia can have significant implica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.08.049
更新日期：2007-11-14 00:00:00
abstract：:Previous electrophysiological as well as imaging research has contributed to the understanding of impairments in attention, executive functions, and memory in patients with attention-deficit/hyperactivity disorder (ADHD). However, there is a lack of studies investigating ADHD related differences in the gamma range of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.06.023
更新日期：2008-10-15 00:00:00
abstract：:Coupling between lumbar motoneurons in the isolated frog spinal cord was studied by using intracellular recordings and intracellular injections of Lucifer Yellow CH. Physiological studies revealed intra- and intersegmental, short latency electrical interactions between many motoneurons. Injections of Lucifer Yellow in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90721-7
更新日期：1983-09-12 00:00:00
abstract：:We used rabbit antisera against manganese (Mn)-superoxide dismutase for immunohistochemical studies of localization in the rat neostriatum. Immunostaining was intense in large-sized neurons and several medium-sized neurons, but it was moderate to weak in other cells. Double immunostaining with monoclonal antibody to c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90618-6
更新日期：1991-05-17 00:00:00
abstract：:The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00372-7
更新日期：1997-07-11 00:00:00
abstract：:Monkeys can gradually increase or decrease the amplitude of the segmentally mediated spinal stretch reflex (SSR) without change in initial muscle length or background EMG activity. Both increase (under the SSR increases mode) and decrease (under the SSR decreases mode) occur slowly, progressing steadily over weeks. Th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90259-7
更新日期：1983-11-14 00:00:00
abstract：:The morphological characteristics, distribution and neurochemical phenotype of striatal neuronal subtypes expressing the GABA(A) receptor alpha3-subunit were investigated using immunocytochemical and immunofluorescent techniques with an antibody specific for this subunit. alpha3-immunopositive neurons were infrequent ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02636-6
更新日期：2000-09-08 00:00:00
abstract：:Reciprocal interactions between nitrergic and dopaminergic systems play a key role in the control of motor behavior. In the present study, we performed a comparative analysis of motor behavior (locomotor activity, catalepsy, rotational behavior) and monoamine metabolism in the striatum and substantia nigra of unilater...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.08.006
更新日期：2015-11-02 00:00:00
