Abstract:
:β-Arrestins are multifunctional adaptor proteins best know for their vital role in regulating G protein coupled receptor (GPCR) trafficking and signaling. β-arrestin2 recruitment and receptor internalization of corticotropin-releasing factor receptor 1 (CRFR1), a GPCR whose antagonists have been shown to demonstrate both anxiolytic- and antidepressant-like effects, have previously been shown to be modulated by PDZ proteins. Thus, a structural characterization of the interaction between β-arrestins and PDZ proteins can delineate potential mechanism of PDZ-dependent regulation of GPCR trafficking. Here, we find that the PDZ proteins PSD-95, MAGI1, and PDZK1 interact with β-arrestin2 in a PDZ domain-dependent manner. Further investigation of such interaction using mutational analyses revealed that mutating the alanine residue at 175 residue of β-arrestin2 to phenylalanine impairs interaction with PSD-95. Additionally, A175F mutant of β-arrestin2 shows decreased CRF-stimulated recruitment to CRFR1 and reduced receptor internalization. Thus, our findings show that the interaction between β-arrestins and PDZ proteins is key for CRFR1 trafficking and may be targeted to mitigate impaired CRFR1 signaling in mental and psychiatric disorders.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Gupta S,Abd-Elrahman KS,Albaker A,Dunn HA,Ferguson SSGdoi
10.1016/j.cellsig.2019.109361subject
Has Abstractpub_date
2019-11-01 00:00:00pages
109361eissn
0898-6568issn
1873-3913pii
S0898-6568(19)30157-3journal_volume
63pub_type
杂志文章abstract::Integrin β6 (ITGB6), an epithelial-specific integrin, is upregulated in oral squamous cell carcinomas (OSCC) and is associated with progression and metastasis of OSCC. Lysophosphatidic acid (LPA), an important bioactive phospholipid present in saliva, has also been related to OSCC cell migration and invasiveness. LPA ...
journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2010.12.003
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doi:10.1016/0898-6568(93)90038-n
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2019.109504
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2015.11.008
更新日期:2016-02-01 00:00:00
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2014.03.014
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2015.08.009
更新日期:2015-11-01 00:00:00
abstract::G protein-coupled receptor kinases (GRKs) are key modulators of G protein-coupled receptor signalling. Increasing evidence points to the occurrence of complex mechanisms able to modulate the subcellular localization, activity and expression levels of GRKs, revealing new functional interactions of these kinases with di...
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pub_type: 杂志文章,评审
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2013.05.020
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