Lysophosphatidic acid induces Ca2+ mobilization and c-Myc expression in mouse embryonic stem cells via the phospholipase C pathway.


:Embryonic stem cells (ESC) are pluripotent and could be maintained in vitro in a self-renewing state indefinitely, at the same time preserving their potential to differentiate towards more specific lineages. Despite the progress in the field, the complex network of signalling cascades involved in the maintenance of the self-renewing and pluripotent state remains not fully understood. In the present study, we have investigated the role of lysophosphatidic acid (LPA), a potent mitogen present in serum, in Ca(2+) signalling and early gene activation in mouse ESC (mESC). In these cells, we detected the expression of the G-protein coupled LPA receptor subtypes LPA(1), LPA(2) and LPA(3). Using fluorescence Ca(2+) imaging techniques, we showed that LPA induced an increase in intracellular Ca(2+) concentration. This increase was also observed in the absence of extracellular Ca(2+), suggesting the involvement of internal stores. Pre-treatment with BAPTA-AM, thapsigargin or U-73122 efficiently blocked this Ca(2+) release, indicating that LPA was evoking Ca(2+) mobilization from the endoplasmic reticulum via the phospholipase C (PLC) pathway. Interestingly, this signalling cascade initiated by LPA was involved in inducing the expression of the Ca(2+)-dependent early response gene c-myc, a key gene implicated in ESC self-renewal and pluripotency. Additionally, LPA increased the proliferation rate of mESC. Our findings therefore outline the physiological role of LPA in mESC.


Cell Signal


Cellular signalling


Todorova MG,Fuentes E,Soria B,Nadal A,Quesada I




Has Abstract


2009-04-01 00:00:00














  • The SH3 domain of the S. cerevisiae Cdc25p binds adenylyl cyclase and facilitates Ras regulation of cAMP signalling.

    abstract::Cdc25 and Ras are two proteins required for cAMP signalling in the budding yeast Saccharomyces cerevisiae. Cdc25 is the guanine nucleotide exchange protein that activates Ras. Ras, in turn, activates adenylyl cyclase. Cdc25 has a Src homology 3 (SH3) domain near the N-terminus and a catalytic domain in the C-terminal ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Mintzer KA,Field J

    更新日期:1999-02-01 00:00:00

  • Mechanisms of action of antidepressants: from neurotransmitter systems to signaling pathways.

    abstract::Antidepressants are commonly used in the treatment of anxiety and depression, medical conditions that affect approximately 17-20% of the population. The clinical effects of antidepressants take several weeks to manifest, suggesting that these drugs induce adaptive changes in brain structures affected by anxiety and de...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Taylor C,Fricker AD,Devi LA,Gomes I

    更新日期:2005-05-01 00:00:00

  • Enteropathogenic Escherichia coli Tir recruits cellular SHP-2 through ITIM motifs to suppress host immune response.

    abstract::Immune responses to pathogens are regulated by immune receptors containing either an immunoreceptor tyrosine-based activation motif (ITAM) or an immunoreceptor tyrosine-based inhibitory motif (ITIM). The important diarrheal pathogen enteropathogenic Escherichia coli (EPEC) require delivery and insertion of the bacteri...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Yan D,Quan H,Wang L,Liu F,Liu H,Chen J,Cao X,Ge B

    更新日期:2013-09-01 00:00:00

  • Involvement of store-operated calcium signaling in EGF-mediated COX-2 gene activation in cancer cells.

    abstract::Growing evidence shows that chronic inflammation drives the progression of colorectal cancer (CRC). Cyclooxygenase-2 (COX-2) is one of the most important inflammatory genes involved in solid tumor metastasis. Epidermal growth factor receptor (EGFR) also plays a key role in cancer cell development. We compared the expr...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wang JY,Chen BK,Wang YS,Tsai YT,Chen WC,Chang WC,Hou MF,Wu YC,Chang WC

    更新日期:2012-01-01 00:00:00

  • MiR-16 targets Bcl-2 in paclitaxel-resistant lung cancer cells and overexpression of miR-16 along with miR-17 causes unprecedented sensitivity by simultaneously modulating autophagy and apoptosis.

    abstract::Non-small cell lung cancer is one of the most aggressive cancers as per as the mortality and occurrence is concerned. Paclitaxel based chemotherapeutic regimes are now used as an important option for the treatment of lung cancer. However, resistance of lung cancer cells to paclitaxel continues to be a major clinical p...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Chatterjee A,Chattopadhyay D,Chakrabarti G

    更新日期:2015-02-01 00:00:00

  • Early growth response gene 1, a TRBP binding protein, is involved in miRNA activity of miR-125a-3p in human cells.

    abstract:BACKGROUND:MicroRNAs (miRNAs) are key regulators of many cellular pathways. However, the picture for components or regulators involved in the process of miRNA biogenesis and function remains to be further elucidated. Early growth response gene 1 (Egr1) has long been considered as tumor suppressor and transcriptional fa...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wei J,Ouyang Y,Li X,Zhu B,Yang J,Cui Y,Chen X,Lin F,Long M,Yang A,Dong K,Zhang H

    更新日期:2015-06-01 00:00:00

  • Overexpression of p75(NTR) increases survival of breast cancer cells through p21(waf1).

    abstract::The p75 neurotrophin receptor (p75(NTR)) plays a critical role in various neuronal and non-neuronal cell types by regulating cell survival, differentiation and proliferation. To evaluate the influence of p75(NTR) in breast cancer development, we have established and characterized breast cancer cells which stably overe...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Verbeke S,Meignan S,Lagadec C,Germain E,Hondermarck H,Adriaenssens E,Le Bourhis X

    更新日期:2010-12-01 00:00:00

  • Apremilast is a selective PDE4 inhibitor with regulatory effects on innate immunity.

    abstract::Apremilast, an oral small molecule inhibitor of phosphodiesterase 4 (PDE4), is in development for chronic inflammatory disorders, and has shown efficacy in psoriasis, psoriatic arthropathies, and Behçet's syndrome. In March 2014, the US Food and Drug Administration approved apremilast for the treatment of adult patien...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Schafer PH,Parton A,Capone L,Cedzik D,Brady H,Evans JF,Man HW,Muller GW,Stirling DI,Chopra R

    更新日期:2014-09-01 00:00:00

  • T-cadherin attenuates the PERK branch of the unfolded protein response and protects vascular endothelial cells from endoplasmic reticulum stress-induced apoptosis.

    abstract::Endoplasmic reticulum (ER) stress activated by perturbations in ER homeostasis induces the unfolded protein response (UPR) with chaperon Grp78 as the key activator of UPR signalling. The aim of UPR is to restore normal ER function; however prolonged or severe ER stress triggers apoptosis of damaged cells to ensure pro...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Kyriakakis E,Philippova M,Joshi MB,Pfaff D,Bochkov V,Afonyushkin T,Erne P,Resink TJ

    更新日期:2010-09-01 00:00:00

  • CRH suppressed TGFβ1-induced Epithelial-Mesenchymal Transition via induction of E-cadherin in breast cancer cells.

    abstract::Since its discovery in biopsies from breast cancer patients, the effect of corticotropin-releasing hormone (CRH) on carcinoma progression is still unclear. Transforming growth factorβ1 (TGFβ1) promotes Epithelial-Mesenchymal Transition (EMT) and induces Snail1 and Twist1 expressions. Loss of epithelial cadherin (E-cad...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Jin L,Chen J,Li L,Li C,Chen C,Li S

    更新日期:2014-04-01 00:00:00

  • Multi-mechanisms are involved in reactive oxygen species regulation of mTORC1 signaling.

    abstract::The mammalian target of rapamycin complex 1(mTORC1) integrates diverse signals to control cell growth, proliferation, survival, and metabolism. Role of reactive oxygen species (ROS) on mTORC1 signaling remains obscure and mechanisms through which ROS modulate mTORC1 are not known.We demonstrate that low doses ROS expo...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Li M,Zhao L,Liu J,Liu A,Jia C,Ma D,Jiang Y,Bai X

    更新日期:2010-10-01 00:00:00

  • E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3.

    abstract::Tribbles 3 homolog (TRB3) is recently identified as a scaffold-like regulator of various signal transducers and has been implicated in several processes including insulin signaling, NF-kappaB signaling, lipid metabolism and BMP signaling. To further understand cellular mechanisms of TRB3 regulation, we performed a yea...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Zhou Y,Li L,Liu Q,Xing G,Kuai X,Sun J,Yin X,Wang J,Zhang L,He F

    更新日期:2008-05-01 00:00:00

  • Neutrophil signaling during myocardial infarction wound repair.

    abstract::Neutrophils are key effector cells of the innate immune system, serving as a first line of defense in the response to injury and playing essential roles in the wound healing process. Following myocardial infarction (MI), neutrophils infiltrate into the infarct region to propagate inflammation and begin the initial pha...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Daseke MJ 2nd,Chalise U,Becirovic-Agic M,Salomon JD,Cook LM,Case AJ,Lindsey ML

    更新日期:2021-01-01 00:00:00

  • Ligand activation of peroxisome proliferator-activated receptor-beta/delta(PPARbeta/delta) inhibits cell growth of human N/TERT-1 keratinocytes.

    abstract::The functional role of peroxisome proliferator-activated receptor-beta(PPARbeta; also referred to as PPARdelta) in epidermal cell growth remains controversial. Recent evidence suggests that ligand activation of PPARbeta/delta increases cell growth and inhibits apoptosis in epidermal cells. In contrast, other reports s...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Burdick AD,Bility MT,Girroir EE,Billin AN,Willson TM,Gonzalez FJ,Peters JM

    更新日期:2007-06-01 00:00:00

  • Proteasome inhibitor-I enhances tunicamycin-induced chemosensitization of prostate cancer cells through regulation of NF-κB and CHOP expression.

    abstract::Although endoplasmic reticulum (ER) stress induction by some anticancer drugs can lead to apoptotic death of cancer cells, combination therapy with other chemicals would be much more efficient. It has been reported that proteasome inhibitors could induce cancer cell death through ER-stress. Our study, however, showed ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Huong PT,Moon DO,Kim SO,Kim KE,Jeong SJ,Lee KW,Lee KS,Jang JH,Erikson RL,Ahn JS,Kim BY

    更新日期:2011-05-01 00:00:00

  • Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.

    abstract::SCF induces autophosphorylation of Kit and activates a variety of signaling components including Jnks, Erks, PI 3 Kinase, the JAK-Stat pathway and members of the Src family. Previously we showed that Lyn is activated at multiple points during SCF-induced cell cycle progression and contributes to SCF-mediated growth, c...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Shivakrupa R,Linnekin D

    更新日期:2005-01-01 00:00:00

  • Illumina-microarray analysis of mycophenolic acid-induced cell death in an insulin-producing cell line and primary rat islet cells: new insights into apoptotic pathways involved.

    abstract::Mycophenolic acid (MPA), widely used to prevent organ transplant rejection, may induce toxicity and impair function in beta-cells. Mechanisms of MPA-induced cell death have not been fully explored. In this study, we examined gene expression patterns in INS-1E cells and isolated primary rat islets following MPA treatme...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Park YJ,Ahn HJ,Kim YS,Cho Y,Joo DJ,Ju MK

    更新日期:2010-11-01 00:00:00

  • Synergistic effect of endothelin-1 and cyclic AMP on glucose transport in 3T3-L1 adipocytes.

    abstract::We have demonstrated previously that chronic exposure to endothlin-1 enhances glucose transport in 3T3-L1 adipocytes via augmented GLUT1 mRNA and protein accumulation. In the present study, we further examined the combined effect of endothelin-1 (ET-1) and cAMP on glucose transport. In cells pretreated with ET-1 and 8...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Fong JC,Kao YS,Tsai HY,Chiou YY,Chiou GY

    更新日期:2004-07-01 00:00:00

  • Cell-surface heparan sulfate proteoglycans as multifunctional integrators of signaling in cancer.

    abstract::Proteoglycans (PGs) represent a large proportion of the components that constitute the extracellular matrix (ECM). They are a diverse group of glycoproteins characterized by a covalent link to a specific glycosaminoglycan type. As part of the ECM, heparan sulfate (HS)PGs participate in both physiological and pathologi...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Hassan N,Greve B,Espinoza-Sánchez NA,Götte M

    更新日期:2021-01-01 00:00:00

  • Imatinib exhibit synergistic pleiotropy in the prevention of colorectal cancer by suppressing proinflammatory, cell survival and angiogenic signaling.

    abstract::Recent global incidences and mortality rates have placed colorectal cancer (CRC) at third and second positions, respectively, among both sexes of all ages. Resistance during chemotherapy is a big problem in the treatment and disease-free survival of CRC patients. Discovery of new anticancer drug(s) is a time taking pr...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Dhiman DK,Sanyal SN,Vaish V

    更新日期:2020-12-01 00:00:00

  • Identification of distinct signalling pathways for somatostatin receptors SSTR1 and SSTR2 as revealed by microphysiometry.

    abstract::Somatostatin receptors (SSTRs) are known to mediate diverse cellular responses. Most target cell express more than one SSTR isoform, making it difficult to define the signalling pathway used by individual receptor subtypes. Thus, we have expressed SSTR1 or SSTR2 in rat pituitary F4C1 cells which lack endogenous SSTRs....

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Chen L,Tashjian AH Jr

    更新日期:1999-07-01 00:00:00

  • The Na+, K(+)-ATPase inhibitor ouabain and the Na+ ionophore monensin have opposite effects upon carbachol-stimulated inositol phospholipid breakdown in rat cerebral cortical miniprisms.

    abstract::The effects of ouabain and monensin upon basal and carbachol-stimulated inositol phospholipid breakdown in rat cerebral cortical miniprisms have been investigated. Basal inositol phospholipid breakdown was increased by both compounds at both 6 and 18 mM K+. Enhancement of the carbachol response at 6 mM, but not at 18 ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Tiger G,Fowler CJ

    更新日期:1991-01-01 00:00:00

  • Detection of calmodulin-binding proteins and calmodulin-dependent phosphorylation linked to calmodulin-dependent chemotaxis to folic and cAMP in Dictyostelium.

    abstract::Calmodulin (CaM) antagonists, trifluoperazine (TFP) or calmidazolium (R24571), dose-dependently inhibited cAMP and folic acid (FA) chemotaxis in Dictyostelium. Developing, starved, and refed cells were compared to determine if certain CaM-binding proteins (CaMBPs) and CaM-dependent phosphorylation events could be iden...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Gauthier ML,O'Day DH

    更新日期:2001-08-01 00:00:00

  • Interaction between TCL1 and Epac1 in the activation of Akt kinases in plasma membranes and nuclei of 8-CPT-2-O-Me-cAMP-stimulated macrophages.

    abstract::Epac1 is a cAMP-stimulated guanine exchange factor that activates Rap1. The protein product of the T cell leukemia 1 (TCL1) proto-oncogene binds to Akt enhancing its kinase activity. TCL1 and Epac promote cellular proliferation because of their activating effects on Akt. Employing macrophages, we have studied the mech...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Misra UK,Kaczowka SJ,Pizzo SV

    更新日期:2008-01-01 00:00:00

  • The many faces of compartmentalized PKA signalosomes.

    abstract::Cellular signal transmission requires the dynamic formation of spatiotemporally controlled molecular interactions. At the cell surface information is received by receptor complexes and relayed through intracellular signaling platforms which organize the actions of functionally interacting signaling enzymes and substra...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Torres-Quesada O,Mayrhofer JE,Stefan E

    更新日期:2017-09-01 00:00:00

  • Role of mitogen-activated protein kinase signaling in the pathogenesis of dengue virus infection.

    abstract::Dengue virus (DENV) infection is a disease that is endemic to many parts of the world, and its increasing prevalence ranks it among the diseases considered to be a significant threat to public health. The clinical manifestations of DENV infection range from mild dengue fever (DF) to more severe dengue hemorrhagic feve...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Sreekanth GP,Yenchitsomanus PT,Limjindaporn T

    更新日期:2018-08-01 00:00:00

  • Translocated in liposarcoma (TLS) is a substrate for fibroblast growth factor receptor-1.

    abstract::Binding of fibroblast growth factor (FGF) to the high affinity receptor-1 (FGFR-1) leads to activation of its endogenous tyrosine kinase activity. A number of substrates for the FGFR-1 kinase have been identified. Among those, FGF receptor-substrate-2 (FRS-2) was identified by virtue of its interaction with p13suc, a ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Klint P,Hellman U,Wernstedt C,Aman P,Ron D,Claesson-Welsh L

    更新日期:2004-04-01 00:00:00

  • GSK3beta inhibition promotes melanogenesis in mouse B16 melanoma cells and normal human melanocytes.

    abstract::Glycogen synthase kinase 3beta (GSK3beta) is implicated in many biological events, including embryonic development, cell differentiation, apoptosis, and the insulin response. GSK3beta also plays a key role in the Wnt/beta-catenin pathway. The master regulator of the pigmentation microphthalmia-associated transcription...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Bellei B,Flori E,Izzo E,Maresca V,Picardo M

    更新日期:2008-10-01 00:00:00

  • The dual role of S-nitrosoglutathione (GSNO) during thymocyte apoptosis.

    abstract::Nitric oxide (NO) is known to regulate redox-sensitive signalling pathways in physiology and pathophysiology. Depending on its concentration, the NO-releasing compound S-nitrosoglutathione (GSNO) causes negative and positive regulation of thymocyte apoptosis. At levels below 0.6 mM, GSNO produces deoxyribonucleic acid...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Sandau K,Brüne B

    更新日期:1996-03-01 00:00:00

  • GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication.

    abstract::In eukaryotes, proper loading and activation of MCM helicase at chromosomal origins plays a central role in DNA replication. Activation of MCM helicase requires its association with CDC45-GINS complex, but the mechanism of how this complex activates MCM helicase is poorly understood. Here we identified SIK1 (salt-indu...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Joshi K,Shah VJ,Maddika S

    更新日期:2016-12-01 00:00:00