当前位置： SCI文献检索 > CELLULAR SIGNALLING期刊下所有文献 > The signalling profile of recombinant human orexin-2 receptor.

The signalling profile of recombinant human orexin-2 receptor.

Abstract：

:Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, OX1R and OX2R, which activate multiple G-proteins. In many tissues, orexins activate extracellular signal-regulated kinase (ERK(1/2)) and p38 mitogen-activated protein kinase (MAPK); however, the mechanism by which OX2R alone mediates MAPK activation is not understood. This study describes the intracellular signalling pathways involved in OX2R-mediated ERK(1/2) and p38 MAPK activation. In HEK-293 cells stably over-expressing recombinant human OX2R, orexin-A/B resulted in a rapid, dose and time dependent increase in activation of ERK(1/2) and p38 MAPK, with maximal activation at 10 min for ERK(1/2) and 30 min for p38 MAPK. Using dominant-negative G-proteins and selective inhibitors of intracellular signalling cascades, we determined that orexin-A and orexin-B induced ERK(1/2) and p38 MAPK activation through multiple G-proteins and different intracellular signalling pathways. ERK(1/2) activation involves Gq/phospholipase C (PLC)/protein kinase C (PKC), Gs/adenylyl cyclase (AC)/cAMP/protein kinase A (PKA) and Gi cascades; however, the Gq/PLC/PKC pathway, as well as PKA is not required for OX2R-mediated p38 MAPK activation. Interestingly, orexin-B-induced ERK(1/2) activation is predominantly mediated through the Gq/PLC/PKC pathway. In conclusion, this is the first comprehensive signalling study of the human OX2R recombinant receptor, showing ERK(1/2) and p38 MAPK activation are regulated by differential signalling pathways in HEK-293 cells, and that the ERK(1/2) activation is severely affected by naturally occurring mutants associated with narcolepsy. Moreover, it is evident that the human OX2R has ligand specific effects, with orexin-B being more potent in this transfected system and this distinct modulation of the MAPKs through OX2R, may translate to the regulation of diverse biological actions of orexins.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Tang J,Chen J,Ramanjaneya M,Punn A,Conner AC,Randeva HS

doi

10.1016/j.cellsig.2008.05.010

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

1651-61

issue

9

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(08)00155-1

journal_volume

20

pub_type

杂志文章

相关文献

CELLULAR SIGNALLING文献大全
  • Different sensitivities of neutrophil responses to a selective protein kinase C inhibitor Ro 31-8425; redundancy in signal transduction.

    abstract：:Previous studies implicating a role for protein kinase C (PKC) in mediating stimulation of cellular responses by physiological agonists have relied on use of non-specific inhibitors or direct stimulation of PKC by phorbol esters. However, much of this evidence is questionable. Here, we have investigated the effects of...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(96)00097-6

    authors： Merritt JE,Sullivan JA,Tse J,Wilkinson Se,Nixon JS

    更新日期：1997-01-01 00:00:00

  • Cellular signaling in normal and cancerous stem cells.

    abstract：:Self-renewing divisions of normal and cancerous stem cells are responsible for the initiation and maintenance of normal and certain cancerous tissues, respectively. Recent findings suggest that tumor surveillance mechanisms can reduce regenerative capacity and frequency of normal stem cells, thereby contributing to ti...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2007.06.021

    authors： Grinstein E,Wernet P

    更新日期：2007-12-01 00:00:00

  • Mechanism of induction of muscle protein degradation by angiotensin II.

    abstract：:Angiotensin I and II have been shown to directly induce protein degradation in skeletal muscle through an increased activity and expression of the ubiquitin-proteasome proteolytic pathway. This investigation determines the role of the nuclear transcription factor nuclear factor-kappaB (NF-kappaB) in this process. Usin...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2005.09.009

    authors： Russell ST,Wyke SM,Tisdale MJ

    更新日期：2006-07-01 00:00:00

  • Regulation of stretch-induced JNK activation by stress fiber orientation.

    abstract：:Cyclic mechanical stretch associated with pulsatile blood pressure can modulate cytoskeletal remodeling and intracellular signaling in vascular endothelial cells. The aim of this study was to evaluate the role of stretch-induced actin stress fiber orientation in intracellular signaling involving the activation of c-ju...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2006.02.008

    authors： Kaunas R,Usami S,Chien S

    更新日期：2006-11-01 00:00:00

  • Liver X receptor inhibits the growth of hepatocellular carcinoma cells via regulating HULC/miR-134-5p/FOXM1 axis.

    abstract：:Hepatocellular carcinoma (HCC) is one of the most common malignancies with a high rate of mortality. Highly upregulated in liver cancer (HULC), the specifically overexpressed long non-coding RNA in human HCC, plays important roles in promoting the growth and metastasis of HCC cells. So downregulating HULC will be bene...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2020.109720

    authors： He J,Yang T,He W,Jiang S,Zhong D,Xu Z,Wei Q,Zhang Y,Shi C

    更新日期：2020-10-01 00:00:00

  • The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling.

    abstract：:Previous reports have suggested that the two mitogen-activated protein kinases (MAPKs) in Dictyostelium discoideum, ERK1 and ERK2, can be directly activated in response to external cAMP even though these MAPKs play different roles in the developmental life cycle. To better characterize MAPK regulation, the levels of p...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2014.10.009

    authors： Schwebs DJ,Hadwiger JA

    更新日期：2015-01-01 00:00:00

  • Validation of highly selective sphingosine kinase 2 inhibitors SLM6031434 and HWG-35D as effective anti-fibrotic treatment options in a mouse model of tubulointerstitial fibrosis.

    abstract：:Renal fibrosis is characterized by chronic inflammation and excessive accumulation of extracellular matrix and progressively leads to functional insufficiency and even total loss of kidney function. In this study we investigated the anti-fibrotic potential of two highly selective and potent SK2 inhibitors, SLM6031434 ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2020.109881

    authors： Schwalm S,Beyer S,Hafizi R,Trautmann S,Geisslinger G,Adams DR,Pyne S,Pyne N,Schaefer L,Huwiler A,Pfeilschifter J

    更新日期：2021-03-01 00:00:00

  • Platelet-derived growth factor (PDGF) induces pulmonary vascular remodeling through 15-LO/15-HETE pathway under hypoxic condition.

    abstract：:15-lipoxygenase (15-LO) is known to play an important role in chronic pulmonary hypertension. Accumulating evidence for its down-stream participants in the vasoconstriction and remodeling processes of pulmonary arteries, while how hypoxia regulates 15-LO/15-hydroxyeicosatetraenoic acid (15-HETE) to mediate hypoxic pul...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2012.06.007

    authors： Zhang L,Ma J,Shen T,Wang S,Ma C,Liu Y,Ran Y,Wang L,Liu L,Zhu D

    更新日期：2012-10-01 00:00:00

  • Type I interferons activate apoptosis in a Jurkat cell variant by caspase-dependent and independent mechanisms.

    abstract：:Although the antiviral actions of interferons (IFNs) are observed in most types of cells, the antiproliferative effects of IFNalpha/beta are variable as are the mechanisms of growth inhibition that may or may not be due to the induction of apoptosis. To understand more about the mechanisms that are responsible for IFN...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2005.10.008

    authors： Gamero AM,Potla R,Sakamoto S,Baker DP,Abraham R,Larner AC

    更新日期：2006-08-01 00:00:00

  • The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins.

    abstract：:The orthologous proteins of the stress-activated protein kinase-interacting 1 (Sin1) family have been implicated in several different signal transduction pathways. In this study, we have investigated the function of the full-length human Sin1 protein and a C-terminally truncated isoform, Sin1alpha, which is produced b...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.10.015

    authors： Schroder W,Bushell G,Sculley T

    更新日期：2005-06-01 00:00:00

  • GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication.

    abstract：:In eukaryotes, proper loading and activation of MCM helicase at chromosomal origins plays a central role in DNA replication. Activation of MCM helicase requires its association with CDC45-GINS complex, but the mechanism of how this complex activates MCM helicase is poorly understood. Here we identified SIK1 (salt-indu...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2016.08.018

    authors： Joshi K,Shah VJ,Maddika S

    更新日期：2016-12-01 00:00:00

  • Granzyme B induction signalling pathway in acute myeloid leukemia cell lines stimulated by tumor necrosis factor alpha and Fas ligand.

    abstract：:Acute myeloid leukemia (AML) cell lines treated by genotoxic agents or by Tumor Necrosis Factor alpha (TNFalpha) acquire potent cytotoxicity towards myeloid cells through activation of granzyme B (GrB)/perforin (PFN) system. Here we first extend this observation to another death receptor activator, Fas Ligand (FasL). ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2006.12.005

    authors： Guilloton F,Jean C,de Thonel A,Laurent G,Quillet-Mary A

    更新日期：2007-06-01 00:00:00

  • SUMO2 overexpression enhances the generation and function of interleukin-17-producing CD8⁺ T cells in mice.

    abstract：:Small ubiquitin-like modifier (SUMO) 2 is a small protein that controls the activity and stability of other proteins by SUMOylation. In this study, T cell-specific SUMO2 overexpressing transgenic mice were generated to study the effect of SUMO2 on T lymphocytes. SUMO2 overexpression promoted differentiation of interle...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2015.03.001

    authors： Won TJ,Lee YJ,Hyung KE,Yang E,Sohn UD,Min HY,Lee DI,Park SY,Hwang KW

    更新日期：2015-06-01 00:00:00

  • Role of the IRF-1 enhancer domain in signalling polyubiquitination and degradation.

    abstract：:The interferon regulated transcription factor IRF-1 is a tumour suppressor protein that is activated in response to viral infection and cell signalling activated by double stranded DNA lesions. IRF-1 has a short half-life (t(0.5) 20-40 min) allowing rapid changes in steady state levels by modulating its rate of degrad...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.05.004

    authors： Pion E,Narayan V,Eckert M,Ball KL

    更新日期：2009-10-01 00:00:00

  • Apoptosis induced by cAMP requires Smac/DIABLO transcriptional upregulation.

    abstract：:Smac/DIABLO is a mitochondrial protein that participates in apoptotic cell death by means of sequestering several members of the inhibitor of apoptosis protein family. This action allows caspase activation, cleavage of key cellular substrates and death. Release from mitochondria is considered the main regulatory step ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2007.01.001

    authors： Martinez-Velazquez M,Melendez-Zajgla J,Maldonado V

    更新日期：2007-06-01 00:00:00

  • Multi-mechanisms are involved in reactive oxygen species regulation of mTORC1 signaling.

    abstract：:The mammalian target of rapamycin complex 1(mTORC1) integrates diverse signals to control cell growth, proliferation, survival, and metabolism. Role of reactive oxygen species (ROS) on mTORC1 signaling remains obscure and mechanisms through which ROS modulate mTORC1 are not known.We demonstrate that low doses ROS expo...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2010.05.015

    authors： Li M,Zhao L,Liu J,Liu A,Jia C,Ma D,Jiang Y,Bai X

    更新日期：2010-10-01 00:00:00

  • Rapid non-genomic actions of progesterone stimulate Ca2+ influx and the acrosome reaction in human sperm.

    abstract：:This review summarizes some recent findings in human sperm which show that progesterone and 17 alpha hydroxyprogesterone are able rapidly (within seconds) to elevate [Ca2+]i and elicit the acrosome reaction (AR) via a non-genomic cell surface receptor. Progesterone promotes a transient elevation in [Ca2+]i which is bl...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/0898-6568(93)90048-q

    authors： Blackmore PF

    更新日期：1993-09-01 00:00:00

  • Regulation of the CCN genes by vitamin D: A possible adjuvant therapy in the treatment of cancer and fibrosis.

    abstract：:The CCN family is composed of six cysteine-rich, modular, and conserved proteins whose functions span a variety of tissues and include cell proliferation, adhesion, angiogenesis, and wound healing. Roles for the CCN proteins throughout the entire body including the skin, kidney, brain, blood vessels, hematopoietic com...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2016.07.009

    authors： Piszczatowski RT,Lents NH

    更新日期：2016-10-01 00:00:00

  • The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.

    abstract：:Macrophages take up oxidized low density lipoprotein (oxLDL) after being exposed to it in the blood vessels. oxLDL transforms macrophages into foam cells, which are a hallmark of atherosclerosis. The effects that oxLDL have on the inflammatory responses of foam cells are not clear. Here, we investigated how oxLDL modu...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2012.02.001

    authors： Min KJ,Cho KH,Kwon TK

    更新日期：2012-06-01 00:00:00

  • Native and iron-saturated bovine lactoferrin differently hinder migration in a model of human glioblastoma by reverting epithelial-to-mesenchymal transition-like process and inhibiting interleukin-6/STAT3 axis.

    abstract：:Glioblastoma, the most lethal form of brain cancer, is characterized by fast growth, migration and invasion of the surrounding parenchyma, with epithelial-to-mesenchymal transition (EMT)-like process being mostly responsible for tumour spreading and dissemination. A number of actors, including cadherins, vimentin, tra...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2019.109461

    authors： Cutone A,Colella B,Pagliaro A,Rosa L,Lepanto MS,Bonaccorsi di Patti MC,Valenti P,Di Bartolomeo S,Musci G

    更新日期：2020-01-01 00:00:00

  • Deregulation of Tpl2 and NF-kappaB signaling and induction of macrophage apoptosis by the anti-depressant drug lithium.

    abstract：:Lithium is an anti-depressant drug that also possesses immunomodulatory functions. The anti-inflammatory effect of lithium is thought to involve activation of the transcription factor CREB, although the underlying mechanism is incompletely understood. We show here that in macrophages lithium stimulates Tpl2, a MAP kin...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.12.010

    authors： Zhang M,Jin W,Zhou X,Yu J,Lee AJ,Sun SC

    更新日期：2009-04-01 00:00:00

  • C5L2 is required for C5a-triggered receptor internalization and ERK signaling.

    abstract：:C5L2 is a receptor that binds to C5a and belongs to the family of G protein-coupled receptors, but its role in physiological C5a-mediated responses remains under debate. Here we show that, like the canonical C5a receptor C5aR, C5L2 plays a pro-inflammatory role in a murine model of acute experimental colitis. We demon...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2014.02.021

    authors： Hsu WC,Yang FC,Lin CH,Hsieh SL,Chen NJ

    更新日期：2014-07-01 00:00:00

  • TGFbeta-induced GRK2 expression attenuates AngII-regulated vascular smooth muscle cell proliferation and migration.

    abstract：:Through diametric actions, the transforming growth factor beta (TGFbeta) and Angiotensin II (AngII) play important roles in regulating various biological responses such as cell proliferation and migration. Signaling initiated by TGFbeta and AngII occurs through two structurally and functionally distinct receptor super...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.01.037

    authors： Guo J,Chen H,Ho J,Mancini J,Sontag T,Laporte SA,Richard DE,Lebrun JJ

    更新日期：2009-06-01 00:00:00

  • Studies on the effect of diadenlyated nucleotides on calcium mobilization and prostacyclin synthesis in bovine aortic endothelial cells.

    abstract：:Extracellular adenine dinucleotides are modulators of blood vessel tone. We have previously demonstrated that Ap(2)A and Ap(4)A induce the synthesis of nitric oxide (NO) from bovine aortic endothelial cells (BAEC) while Ap(3)A and Ap(5)A do not [FEBS Lett. 427 (1998) 320; Arch. Biochem. Biophys. 364 (1999) 280.]. In t...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(00)00147-9

    authors： Haghiac M,Pojoga LH,Hilderman RH

    更新日期：2001-02-01 00:00:00

  • G93A SOD1 alters cell cycle in a cellular model of Amyotrophic Lateral Sclerosis.

    abstract：:Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative multifactorial disease characterized, like other diseases such as Alzheimer's disease (AD), Parkinson's disease (PD) or frontotemporal dementia (FTD), by the degeneration of specific neuronal cell populations. Motor neuron loss is distinctive of ALS. However, ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2010.05.016

    authors： Cova E,Ghiroldi A,Guareschi S,Mazzini G,Gagliardi S,Davin A,Bianchi M,Ceroni M,Cereda C

    更新日期：2010-10-01 00:00:00

  • ZIP kinase, a key regulator of myosin protein phosphatase 1.

    abstract：:Two major physiological roles have been defined for zipper interacting protein kinase (ZIPK), regulation of apoptosis in non-muscle cells and regulation of Ca(2+) sensitization in smooth muscle. Although much attention has focused on the role of ZIPK in the regulation of apoptotic events, its roles in smooth muscle ar...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2005.05.008

    authors： Haystead TA

    更新日期：2005-11-01 00:00:00

  • Activation of phosphoinositide 3-kinase in response to inflammation and nitric oxide leads to the up-regulation of cyclooxygenase-2 expression and subsequent cell proliferation in mesangial cells.

    abstract：:In this study, we showed that nitric oxide (NO) donors induced the mesangial cell proliferation and cyclooxygenase-2 (COX-2) protein expression in murine mesangial cells. An inflammatory condition [lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma)] could also induce cell proliferation and significantly enhanc...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.11.015

    authors： Sheu ML,Chao KF,Sung YJ,Lin WW,Lin-Shiau SY,Liu SH

    更新日期：2005-08-01 00:00:00

  • Hsp90/Cdc37 assembly modulates TGFβ receptor-II to act as a profibrotic regulator of TGFβ signaling during cardiac hypertrophy.

    abstract：:Cardiac hypertrophy is accompanied by excessive collagen deposition in the heart. Despite painstaking research on this fatal disease, the precise role of molecular chaperones in myocardial fibrosis has not yet been elucidated. In this study, we have analyzed the mechanism by which Heat shock protein 90 (Hsp90)/Cell di...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2015.09.005

    authors： Datta R,Bansal T,Rana S,Datta K,Chattopadhyay S,Chawla-Sarkar M,Sarkar S

    更新日期：2015-12-01 00:00:00

  • A novel role for Gq alpha in alpha-thrombin-mediated mitogenic signalling pathways.

    abstract：:alpha-Thrombin activates several G-proteins including members of the Gq, Gi, and G12/13 families, although the physiological importance of these proteins is still not completely understood. We specifically investigated the role of Gq alpha in modulating alpha-thrombin-induced mitogenesis. In Gqa1 cells, a stable cell ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(01)00279-0

    authors： Gardner A,Phillips-Mason PJ,Raben DM,Baldassare JJ

    更新日期：2002-06-01 00:00:00

  • TMEPAI/PMEPA1 inhibits Wnt signaling by regulating β-catenin stability and nuclear accumulation in triple negative breast cancer cells.

    abstract：:Transmembrane prostate androgen-induced protein (TMEPAI) is a type I transmembrane protein induced by several intracellular signaling pathways such as androgen, TGF-β, EGF, and Wnt signaling. It has been reported that TMEPAI functions to suppress TGF-β and androgen signaling but here, we report a novel function of TME...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2019.03.016

    authors： Amalia R,Abdelaziz M,Puteri MU,Hwang J,Anwar F,Watanabe Y,Kato M

    更新日期：2019-07-01 00:00:00