当前位置： SCI文献检索 > CELLULAR SIGNALLING期刊下所有文献 > Osteoclast-derived miR-23a-5p-containing exosomes inhibit osteogenic differentiation by regulating Runx2.

Osteoclast-derived miR-23a-5p-containing exosomes inhibit osteogenic differentiation by regulating Runx2.

Abstract：

BACKGROUND:Some microRNAs (miRNAs) are involved in osteogenic differentiation. In recent years, increasing evidences have revealed that exosomes contain specific miRNAs. However, the effect and mechanism of miR-23a-5p-containing exosomes in osteoblast remain largely unclear. METHODS:We extracted exosomes from RANKL-induced RAW 264.7 cells, and identified exosomes via transmission electron microscopy, western blot and flow cytometry analysis. In addition, exosome secretion was inhibited by GW4869 and Rab27a siRNAs. miR-23a-5p expression was analyzed by qRT-PCR, and the related protein levels were examined by western blot assay. Furthermore, the number and distribution of osteoclasts were detected by TRAP staining, and early osteogenesis was evaluated by ALP staining. Combination of YAP1 and Runx2 was verified by Co-IP assay, and the regulation of miR-23a-5p and Runx2 was measured by dual luciferase reporter assay. RESULTS:We successfully extracted exosomes from RANKL-induced RAW 264.7 cells, and successfully verified exosomes morphology. We also indicated that miR-23a-5p was highly expressed in exosomes from RANKL-induced RAW 264.7 cells, and osteoclast-derived miR-23a-5p-containing exosomes inhibited osteoblast activity, while its inhibition weakened osteoclasts. In mechanism, we demonstrated that Runx2 was a target gene of miR-23a-5p, YAP interacted with Runx2, and YAP or Runx2 inhibited MT1DP expression. In addition, we proved that knockdown of MT1DP facilitated osteogenic differentiation by regulating FoxA1 and Runx2. CONCLUSIONS:We demonstrated that osteoclast-derived miR-23a-5p-containing exosomes could efficiently suppress osteogenic differentiation by inhibiting Runx2 and promoting YAP1-mediated MT1DP. Therefore, we suggested miR-23a-5p in exosomes might provide a novel mechanism for osteoblast function.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Yang JX,Xie P,Li YS,Wen T,Yang XC

doi

10.1016/j.cellsig.2019.109504

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

109504

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(19)30300-6

journal_volume

70

pub_type

杂志文章

相关文献

CELLULAR SIGNALLING文献大全
  • Differential regulation of RhoA-mediated signaling by the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor: independent modulation of TPalpha signaling by prostacyclin and nitric oxide.

    abstract：:In humans, thromboxane (TX) A(2) signals through the TPalpha and TPbeta isoforms of the TXA(2) receptor that exhibit common and distinct roles. For example, Gq/phospholipase (PL)Cbeta signaling by TPalpha is directly inhibited by the vasodilators prostacyclin and nitric oxide (NO) whereas that signaling by TPbeta is u...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.04.006

    authors： Wikström K,Kavanagh DJ,Reid HM,Kinsella BT

    更新日期：2008-08-01 00:00:00

  • Zfra is an inhibitor of Bcl-2 expression and cytochrome c release from the mitochondria.

    abstract：:Zfra is a small size 31-amino-acid C2H2 zinc finger-like protein, which is known to interact with c-Jun N-terminal kinase 1 (JNK1), WW domain-containing oxidoreductase (WWOX, FOR or WOX1), TNF receptor-associated death domain protein (TRADD) and nuclear factor kappaB (NF-kappaB) during stress response. Here, we show t...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.02.018

    authors： Hsu LJ,Hong Q,Schultz L,Kuo E,Lin SR,Lee MH,Lin YS,Chang NS

    更新日期：2008-07-01 00:00:00

  • IL-2 activation of a PI3K-dependent STAT3 serine phosphorylation pathway in primary human T cells.

    abstract：:Interleukin-2 (IL-2) is the major growth factor of activated T lymphocytes. By inducing cell cycle progression and protection from apoptosis in these cells, IL-2 is involved in the successful execution of an immune response. Upon binding its receptor, IL-2 activates a variety of signal transduction pathways, including...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(03)00003-2

    authors： Fung MM,Rohwer F,McGuire KL

    更新日期：2003-06-01 00:00:00

  • Differential regulation and role of interleukin-1 receptor associated kinase-M in innate immunity signaling.

    abstract：:Toll-like-receptor mediated signaling is finely regulated by a complex intracellular protein network including the interleukin-1 receptor associate kinases (IRAKs). IRAK-4, 1, and 2 may positively regulate innate immunity signaling through the activation of various downstream kinases such as MAPKs. In contrast, IRAK-M...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2007.02.009

    authors： Su J,Xie Q,Wilson I,Li L

    更新日期：2007-07-01 00:00:00

  • TMC8 (EVER2) attenuates intracellular signaling by Zn2+ and Ca2+ and suppresses activation of Cl- currents.

    abstract：:Eight paralogue members form the family of transmembrane channel-like (TMC) proteins that share considerable sequence homology to anoctamin 1 (Ano1, TMEM16A). Ano1 is a Ca(2+) activated Cl(-) channel that is related to head and neck cancer, often caused by human papilloma virus (HPV) infection. Mutations in TMC 6 and ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2014.09.001

    authors： Sirianant L,Ousingsawat J,Tian Y,Schreiber R,Kunzelmann K

    更新日期：2014-12-01 00:00:00

  • Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine.

    abstract：:Macrophages are projected as one of the key players responsible for the progression of cancer. Classically activated (M1) macrophages are pro-inflammatory and have a central role in host defense, while alternatively activated (M2) macrophages are associated with immunosuppression. Macrophages residing at the site of n...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2015.03.013

    authors： Ghosh S,Mukherjee S,Choudhury S,Gupta P,Adhikary A,Baral R,Chattopadhyay S

    更新日期：2015-07-01 00:00:00

  • Mechanisms of regulation of the expression and function of G protein-coupled receptor kinases.

    abstract：:G protein-coupled receptor kinases (GRKs) are key modulators of G protein-coupled receptor signalling. Increasing evidence points to the occurrence of complex mechanisms able to modulate the subcellular localization, activity and expression levels of GRKs, revealing new functional interactions of these kinases with di...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/s0898-6568(03)00099-8

    authors： Penela P,Ribas C,Mayor F Jr

    更新日期：2003-11-01 00:00:00

  • Lysophosphatidic acid induces integrin β6 expression in human oral squamous cell carcinomas cells via LPAR1 coupling to Gαi and downstream SMAD3 and ETS-1 activation.

    abstract：:Integrin β6 (ITGB6), an epithelial-specific integrin, is upregulated in oral squamous cell carcinomas (OSCC) and is associated with progression and metastasis of OSCC. Lysophosphatidic acid (LPA), an important bioactive phospholipid present in saliva, has also been related to OSCC cell migration and invasiveness. LPA ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2019.04.008

    authors： Xu M,Yin H,Cai Y,Huang W,Ji Q,Liu F,Shi S,Deng X

    更新日期：2019-08-01 00:00:00

  • A cell surface inactive mutant of the human lutropin receptor (hLHR) attenuates signaling of wild-type or constitutively active receptors via heterodimerization.

    abstract：:The D405N and Y546F mutations of the human lutropin receptor (hLHR) have previously been shown to partially attenuate hCG-stimulated cAMP synthesis despite normal cell surface expression and hCG binding affinity (Min, L. and Ascoli, M. Mol. Endocrinol. 14:1797-1810, 2000). We now show that these mutations each stabili...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.07.003

    authors： Zhang M,Feng X,Guan R,Hébert TE,Segaloff DL

    更新日期：2009-11-01 00:00:00

  • CIKS (Act1 or TRAF3IP2) mediates high glucose-induced endothelial dysfunction.

    abstract：:Hyperglycemia-induced endothelial dysfunction is characterized by enhanced inflammatory cytokine and adhesion molecule expression, and endothelial-monocyte adhesion. The adapter molecule CIKS (connection to IKK and SAPK/JNK; also known as Act1 or TRAF3IP2) is an upstream regulator of NF-κB and AP-1, and plays a role i...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2012.10.009

    authors： Venkatesan B,Valente AJ,Das NA,Carpenter AJ,Yoshida T,Delafontaine JL,Siebenlist U,Chandrasekar B

    更新日期：2013-01-01 00:00:00

  • PKCδ stimulates macropinocytosis via activation of SSH1-cofilin pathway.

    abstract：:Macropinocytosis is an actin-dependent endocytic mechanism mediating internalization of extracellular fluid and associated solutes into cells. The present study was designed to identify the specific protein kinase C (PKC) isoform(s) and downstream effectors regulating actin dynamics during macropinocytosis. We utilize...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2018.09.018

    authors： Singla B,Lin HP,Ghoshal P,Cherian-Shaw M,Csányi G

    更新日期：2019-01-01 00:00:00

  • Coupling receptor tyrosine kinases to Rho GTPases--GEFs what's the link.

    abstract：:Rho GTPases are molecular switches involved in the regulation of many cellular processes. This review summarizes work examining how stimulation of receptor tyrosine kinases (RTKs) leads to the activation of Rho guanine nucleotide exchange factors (GEFs) and their Rho GTPase substrates. The collective findings strongly...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2006.01.022

    authors： Schiller MR

    更新日期：2006-11-01 00:00:00

  • Structural determinants governing β-arrestin2 interaction with PDZ proteins and recruitment to CRFR1.

    abstract：:β-Arrestins are multifunctional adaptor proteins best know for their vital role in regulating G protein coupled receptor (GPCR) trafficking and signaling. β-arrestin2 recruitment and receptor internalization of corticotropin-releasing factor receptor 1 (CRFR1), a GPCR whose antagonists have been shown to demonstrate b...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2019.109361

    authors： Gupta S,Abd-Elrahman KS,Albaker A,Dunn HA,Ferguson SSG

    更新日期：2019-11-01 00:00:00

  • Priming of calcium mobilization in human neutrophils by granulocyte-macrophage colony-stimulating factor: evidence for an involvement of phospholipase D-derived phosphatidic acid.

    abstract：:Human neutrophils pre-incubated with granulocyte-macrophage-colony-stimulating factor (GM-CSF) exhibit an enhanced mobilization of calcium in response to secondary stimuli such as chemotactic factors. The mechanisms underlying this priming effect of GM-CSF were examined. It was first demonstrated that the additional c...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/0898-6568(91)90040-2

    authors： Naccache PH,Hamelin B,Gaudry M,Bourgoin S

    更新日期：1991-01-01 00:00:00

  • Signalling via the G protein-activated K+ channels.

    abstract：:The inwardly rectifying K+ channels of the GIRK (Kir3) family, members of the superfamily of inwardly rectifying K+ channels (Kir), are important physiological tools to regulate excitability in heart and brain by neurotransmitters, and the only ion channels conclusively shown to be activated by a direct interaction wi...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/s0898-6568(97)00095-8

    authors： Dascal N

    更新日期：1997-12-01 00:00:00

  • GSK3beta inhibition promotes melanogenesis in mouse B16 melanoma cells and normal human melanocytes.

    abstract：:Glycogen synthase kinase 3beta (GSK3beta) is implicated in many biological events, including embryonic development, cell differentiation, apoptosis, and the insulin response. GSK3beta also plays a key role in the Wnt/beta-catenin pathway. The master regulator of the pigmentation microphthalmia-associated transcription...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.06.001

    authors： Bellei B,Flori E,Izzo E,Maresca V,Picardo M

    更新日期：2008-10-01 00:00:00

  • Multiple defects occur in the guanine nucleotide regulatory protein system in liver plasma membranes of obese (fa/fa) but not lean (Fa/Fa) Zucker rats: loss of functional Gi and abnormal Gs function.

    abstract：:Hepatocyte membranes from both lean and obese Zucker rats exhibited adenylate cyclase activity that could be stimulated by glucagon, forskolin, NaF and elevated concentrations of p[NH]ppG. In membranes from lean animals, functional Gi was detected by the ability of low concentrations of p[NH]ppG to inhibit forskolin-a...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/0898-6568(89)90016-8

    authors： Houslay MD,Gawler DJ,Milligan G,Wilson A

    更新日期：1989-01-01 00:00:00

  • Protein tyrosine phosphorylation as a mechanism of signalling in mast cells and basophils.

    abstract：:Tyrosine phosphorylation of proteins is a mechanism of signalling for different receptors and is important for cell growth and differentiation. Mast cells and basophils are secretory cells that play a role in inflammatory and immediate allergic reactions. The activation/aggregation of different surface receptors on th...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/0898-6568(95)00024-j

    authors： Hamawy MM,Mergenhagen SE,Siraganian RP

    更新日期：1995-08-01 00:00:00

  • The focal adhesion targeting sequence is the major inhibitory moiety of Fak-related non-kinase.

    abstract：:Focal adhesion kinase (FAK) plays an important role in integrin-mediated signal transduction pathways and its C-terminal noncatalytic domain Fak-related non-kinase (FRNK), which is autonomously expressed, acts as an inhibitor of FAK. A model has been proposed where FAK and FRNK compete for an essential common binding ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(01)00226-1

    authors： Mortier E,Cornelissen F,van Hove C,Dillen L,Richardson A

    更新日期：2001-12-01 00:00:00

  • Leptin upregulates VEGF in breast cancer via canonic and non-canonical signalling pathways and NFkappaB/HIF-1alpha activation.

    abstract：:High levels of VEGF and leptin are strongly linked to worse prognosis of breast cancer. Leptin signalling upregulates VEGF in human and mouse mammary tumor cells (MT), but the specific molecular mechanisms are largely unknown. Pharmacologic and genetic approaches were used to dissect the mechanism of leptin regulation...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2010.05.003

    authors： Gonzalez-Perez RR,Xu Y,Guo S,Watters A,Zhou W,Leibovich SJ

    更新日期：2010-09-01 00:00:00

  • CCN1/CYR61 overexpression in hepatic stellate cells induces ER stress-related apoptosis.

    abstract：:CCN1/CYR61 is a matricellular protein of the CCN family, comprising six secreted proteins specifically associated with the extracellular matrix (ECM). CCN1 acts as an enhancer of the cutaneous wound healing process by preventing hypertrophic scar formation through induction of myofibroblast senescence. In liver fibros...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2015.10.013

    authors： Borkham-Kamphorst E,Steffen BT,Van de Leur E,Haas U,Tihaa L,Friedman SL,Weiskirchen R

    更新日期：2016-01-01 00:00:00

  • Beneficial effects of PTP1B deficiency on brown adipocyte differentiation and protection against apoptosis induced by pro- and anti-inflammatory stimuli.

    abstract：:Insulin is an inducer of brown fat adipogenesis through the activation of a signalling network that involves positive/negative modulators. Given the importance of brown adipose tissue (BAT) for basal thermogenic energy expenditure, we investigated the role of PTP1B in the acquisition of terminal differentiated phenoty...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.11.019

    authors： Miranda S,González-Rodríguez A,Revuelta-Cervantes J,Rondinone CM,Valverde AM

    更新日期：2010-04-01 00:00:00

  • Roles of the intracellular regions of angiotensin II receptor AT2 in mediating reduction of intracellular cGMP levels.

    abstract：:We have shown previously that the angiotensin II (Ang II) receptor AT2 reduces the intracellular levels of cGMP in Xenopus oocytes when activated by ligand binding, and the C-terminal cytoplasmic tail of the AT2 acts as a negative regulator of this function. Here we report the effects of mutations in the 2nd and 3rd i...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.08.007

    authors： Pulakat L,Rahman S,Gray A,Knowle D,Gavini N

    更新日期：2005-03-01 00:00:00

  • Differential involvement of H- and K-Ras in Raf-1 activation determines the role of calmodulin in MAPK signaling.

    abstract：:We have previously demonstrated that inhibition of calmodulin (CaM) and the concomitant reduction of PI3K interfere with H-Ras-mediated activation of Raf-1 [1]. In the present study, we show that CaM has completely opposite effects on K-Ras-mediated Raf-1 activation. The differential contribution of CaM in the regulat...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.07.018

    authors： Moretó J,Vidal-Quadras M,Pol A,Santos E,Grewal T,Enrich C,Tebar F

    更新日期：2009-12-01 00:00:00

  • RhoA/ROCK I signalling downstream of the P2Y13 ADP-receptor controls HDL endocytosis in human hepatocytes.

    abstract：:Cell surface receptors for high-density lipoprotein (HDL) on hepatocytes are major partners in the regulation of cholesterol homeostasis. We have previously demonstrated on human hepatocytes that apolipoprotein A-I binding to an ectopic F(1)-ATPase stimulates the production of extracellular ADP that activates a P2Y(13...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.09.016

    authors： Malaval C,Laffargue M,Barbaras R,Rolland C,Peres C,Champagne E,Perret B,Tercé F,Collet X,Martinez LO

    更新日期：2009-01-01 00:00:00

  • Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

    abstract：:Phosphodiesterases 4 (PDE4) act as proinflammatory enzymes via degradation of cAMP, whereas PDE4 inhibitors play an anti-inflammatory role in vitro and in vivo. In particular, apremilast has been recently approved for the treatment of psoriasis and psoriatic arthritis. However, little is known on the expression patter...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2016.01.007

    authors： Schafer PH,Truzzi F,Parton A,Wu L,Kosek J,Zhang LH,Horan G,Saltari A,Quadri M,Lotti R,Marconi A,Pincelli C

    更新日期：2016-07-01 00:00:00

  • Activation of CRHR1 contributes to cerebral endothelial barrier impairment via cPLA2 phosphorylation in experimental ischemic stroke.

    abstract：:The activation of corticotrophin-releasing hormone receptor (CRHR) 1 is implicated in neuronal injury in experimental stroke. However, little is known about the relationship between CRHR1 activation and brain endothelial barrier impairment after ischemia and reperfusion (I/R). Recently we have demonstrated that the ac...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2019.109467

    authors： Cao C,Zhou J,Wu X,Qian Y,Hong Y,Mu J,Jin L,Zhu C,Li S

    更新日期：2020-02-01 00:00:00

  • Silencing Daxx increases the anti-tumor activity of a TRAIL/shRNA Bcl-xL-expressing oncolytic adenovirus through enhanced viral replication and cellular arrest.

    abstract：:We previously showed that an increase of cellular Bcl-xL mediates acquired resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and knockdown of Bcl-xL expression greatly sensitized TRAIL-induced cytotoxicity. Here, we show that Daxx downregulation increases the anti-tumorigenic activity throu...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2015.02.028

    authors： Kang S,Kang D,Bakhtiar Ul Islam SM,Je S,Kim JH,Song JJ

    更新日期：2015-06-01 00:00:00

  • MAP kinases have different functions in Dictyostelium G protein-mediated signaling.

    abstract：:Extracellular signal regulated kinases (ERKs) are a class of MAP kinases that function in many signaling pathways in eukaryotic cells and in some cases, a single stimulus can activate more than one ERK suggesting functional redundancy or divergence from a common pathway. Dictyostelium discoideum encodes only two MAP k...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2010.01.008

    authors： Nguyen HN,Raisley B,Hadwiger JA

    更新日期：2010-05-01 00:00:00

  • Signalling aspects of insulin resistance in skeletal muscle: mechanisms induced by lipid oversupply.

    abstract：:A reduced capacity for insulin to elicit increases in glucose uptake and metabolism in target tissues such as skeletal muscle is a common feature of obesity and diabetes. The association between lipid oversupply and such insulin resistance is well established, and evidence for mechanisms through which lipids could pla...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/s0898-6568(00)00110-8

    authors： Schmitz-Peiffer C

    更新日期：2000-10-01 00:00:00