Differential regulation of RhoA-mediated signaling by the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor: independent modulation of TPalpha signaling by prostacyclin and nitric oxide.

Abstract:

:In humans, thromboxane (TX) A(2) signals through the TPalpha and TPbeta isoforms of the TXA(2) receptor that exhibit common and distinct roles. For example, Gq/phospholipase (PL)Cbeta signaling by TPalpha is directly inhibited by the vasodilators prostacyclin and nitric oxide (NO) whereas that signaling by TPbeta is unaffected. Herein, we investigated whether TPalpha and/or TPbeta regulate G(12)/Rho activation and whether that signaling might be differentially regulated by prostacyclin and/or NO. Both TPalpha and TPbeta independently regulated RhoA activation and signaling in clonal cells over-expressing TPalpha or TPbeta and in primary human aortic smooth muscle cells (1 degrees AoSMCs). While RhoA-signaling by TPalpha was directly impaired by prostacyclin and NO through protein kinase (PK)A- and PKG-dependent phosphorylation, respectively, signaling by TPbeta was not directly affected by either agent. Collectively, while TPalpha and TPbeta contribute to RhoA activation, our findings support the hypothesis that TPalpha is involved in the dynamic regulation of haemostasis and vascular tone, such as in response to prostacyclin and NO. Conversely, the role of TPbeta in such processes remains unsolved. Data herein provide essential new insights into the physiologic roles of TPalpha and TPbeta and, through studies in AoSMCs, reveal an additional mode of regulation of VSM contractile responses by TXA(2).

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Wikström K,Kavanagh DJ,Reid HM,Kinsella BT

doi

10.1016/j.cellsig.2008.04.006

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

1497-512

issue

8

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(08)00118-6

journal_volume

20

pub_type

杂志文章
  • The interaction of dystrophin with beta-dystroglycan is regulated by tyrosine phosphorylation.

    abstract::Dystrophin and the dystrophin-associated protein complex (DAPC) have recently been implicated in cell signalling events. These proteins are ideally placed to transduce signals from the extracellular matrix (ECM) to the cytoskeleton. Here we show that beta-dystroglycan is tyrosine-phosphorylated in C2/C4 mouse myotubes...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(01)00188-7

    authors: Ilsley JL,Sudol M,Winder SJ

    更新日期:2001-09-01 00:00:00

  • Studies on the effect of diadenlyated nucleotides on calcium mobilization and prostacyclin synthesis in bovine aortic endothelial cells.

    abstract::Extracellular adenine dinucleotides are modulators of blood vessel tone. We have previously demonstrated that Ap(2)A and Ap(4)A induce the synthesis of nitric oxide (NO) from bovine aortic endothelial cells (BAEC) while Ap(3)A and Ap(5)A do not [FEBS Lett. 427 (1998) 320; Arch. Biochem. Biophys. 364 (1999) 280.]. In t...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(00)00147-9

    authors: Haghiac M,Pojoga LH,Hilderman RH

    更新日期:2001-02-01 00:00:00

  • Structural determinants of LL5beta subcellular localisation and association with filamin C.

    abstract::PI3K signalling pathways link cell surface receptors to the control of several intracellular functions including cell growth, survival and movement. Filamins are important regulators of cortical actin structure and function. LL5beta is a filamin binding protein that is an effector of the PI3K signalling pathway. We de...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2006.10.007

    authors: Paranavitane V,Stephens LR,Hawkins PT

    更新日期:2007-04-01 00:00:00

  • MAP kinases have different functions in Dictyostelium G protein-mediated signaling.

    abstract::Extracellular signal regulated kinases (ERKs) are a class of MAP kinases that function in many signaling pathways in eukaryotic cells and in some cases, a single stimulus can activate more than one ERK suggesting functional redundancy or divergence from a common pathway. Dictyostelium discoideum encodes only two MAP k...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2010.01.008

    authors: Nguyen HN,Raisley B,Hadwiger JA

    更新日期:2010-05-01 00:00:00

  • Soluble and particulate inositol 1,4,5-trisphosphate 5-phosphatases show common antigenic determinants.

    abstract::Inositol 1,4,5-trisphosphate 5-phosphatase catalyses the dephosphorylation of the phosphate in the 5-position from inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate. One particulate and two soluble enzymes were previously described in bovine brain. In this study, we have obtained a precipitating anti...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90082-l

    authors: Verjans B,Hollande F,Moreau C,Lejeune C,Erneux C

    更新日期:1990-01-01 00:00:00

  • Inducible cAMP early repressor (ICER) is a novel regulator of RIG-I mediated IFN-β production.

    abstract::Antiviral responses can be triggered by the cytoplasmic RNA helicase RIG-I that binds to viral RNA. RIG-I-mediated signaling stimulates the transcription factors IRF3 and NF-κB and their activation mechanisms have been intensively studied. Here we examined Sendai virus (SV)-mediated activation of the transcription fac...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.05.014

    authors: Johnsen IB,Bergstroem B,Stiberg KA,Thommesen L,Anthonsen MW

    更新日期:2013-09-01 00:00:00

  • Rapid non-genomic actions of progesterone stimulate Ca2+ influx and the acrosome reaction in human sperm.

    abstract::This review summarizes some recent findings in human sperm which show that progesterone and 17 alpha hydroxyprogesterone are able rapidly (within seconds) to elevate [Ca2+]i and elicit the acrosome reaction (AR) via a non-genomic cell surface receptor. Progesterone promotes a transient elevation in [Ca2+]i which is bl...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/0898-6568(93)90048-q

    authors: Blackmore PF

    更新日期:1993-09-01 00:00:00

  • Unsaturated fatty acids synergistically enhance glucocorticoid-induced gene expression.

    abstract::Regulation by unsaturated fatty acids of glucocorticoid-sensitive gene transcription was studied in HeLa cells transiently transfected with a mouse mammary tumour virus-luciferase reporter gene. Arachidonic acid and docosahexaenoic acid by themselves had no effect on basal levels of luciferase expression. However, the...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(95)00010-m

    authors: Vallette G,Sumida C,Thobie N,Nunez EA

    更新日期:1995-05-01 00:00:00

  • Overexpression of p75(NTR) increases survival of breast cancer cells through p21(waf1).

    abstract::The p75 neurotrophin receptor (p75(NTR)) plays a critical role in various neuronal and non-neuronal cell types by regulating cell survival, differentiation and proliferation. To evaluate the influence of p75(NTR) in breast cancer development, we have established and characterized breast cancer cells which stably overe...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2010.07.014

    authors: Verbeke S,Meignan S,Lagadec C,Germain E,Hondermarck H,Adriaenssens E,Le Bourhis X

    更新日期:2010-12-01 00:00:00

  • Synergistic activation of JNK/SAPK induced by TNF-alpha and IFN-gamma: apoptosis of pancreatic beta-cells via the p53 and ROS pathway.

    abstract::IFN-gamma and TNF-alpha are major proinflammatory cytokines implicated in islet beta-cell destruction, which results in type-1 diabetes; however, the underlying mechanism is not clear. Using pancreatic beta-cell line MIN6N8 cells, co-treatment with TNF-alpha and IFN-gamma, but neither cytokine alone, synergistically i...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2005.03.020

    authors: Kim WH,Lee JW,Gao B,Jung MH

    更新日期:2005-12-01 00:00:00

  • Sphingosine-1-phosphate induces COX-2 expression and PGE2 production in human granulosa cells through a S1P1/3-mediated YAP signaling.

    abstract::Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that can regulate various physiological and pathological processes. The expression of S1P has been detected in human follicular fluid. In addition, two S1P receptors, S1P1 and S1P3, are expressed at a high level in human granulosa cells. Cyclooxygenase-2 (COX-2...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2016.03.006

    authors: Cheng JC,Chang HM,Liu PP,Leung PC

    更新日期:2016-06-01 00:00:00

  • Hypoxia-induced neuronal apoptosis is mediated by de novo synthesis of ceramide through activation of serine palmitoyltransferase.

    abstract::Cellular hypoxia can lead to cell death or adaptation and has important effects on development, physiology, and pathology. Here, we investigated the role and regulation of ceramide in hypoxia-induced apoptosis of SH-SY5Y neuroblastoma cells. Hypoxia increased the ceramide concentration; subsequently, we observed bioch...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.11.015

    authors: Kang MS,Ahn KH,Kim SK,Jeon HJ,Ji JE,Choi JM,Jung KM,Jung SY,Kim DK

    更新日期:2010-04-01 00:00:00

  • The adaptor function of SHP-2 downstream of the prolactin receptor is required for the recruitment of p29, a substrate of SHP-2.

    abstract::SHP-2, a cytosolic protein tyrosine phosphatase with two SH2 domains and multiple tyrosine phosphorylation sites, contributes to signal transduction as an enzyme and/or adaptor molecule. Here we demonstrate that prolactin (PRL) stimulation of the PRL-responsive Nb2 cells, a rat lymphoma cell line, and T47D cells, a hu...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(02)00122-5

    authors: Minoo P,Chughtai N,Campiglio M,Stein-Gerlach M,Lebrun JJ,Ullrich A,Ali S

    更新日期:2003-03-01 00:00:00

  • T cell tyrosine phosphorylation response to transient redox stress.

    abstract::Reactive Oxygen Species (ROS) are crucial to multiple biological processes involved in the pathophysiology of inflammation, and are also involved in redox signaling responses. Although previous reports have described an association between oxidative events and the modulation of innate immunity, a role for redox signal...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.12.014

    authors: Secchi C,Carta M,Crescio C,Spano A,Arras M,Caocci G,Galimi F,La Nasa G,Pippia P,Turrini F,Pantaleo A

    更新日期:2015-04-01 00:00:00

  • The SH3 domain of the S. cerevisiae Cdc25p binds adenylyl cyclase and facilitates Ras regulation of cAMP signalling.

    abstract::Cdc25 and Ras are two proteins required for cAMP signalling in the budding yeast Saccharomyces cerevisiae. Cdc25 is the guanine nucleotide exchange protein that activates Ras. Ras, in turn, activates adenylyl cyclase. Cdc25 has a Src homology 3 (SH3) domain near the N-terminus and a catalytic domain in the C-terminal ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(98)00044-8

    authors: Mintzer KA,Field J

    更新日期:1999-02-01 00:00:00

  • Osteoclast-derived miR-23a-5p-containing exosomes inhibit osteogenic differentiation by regulating Runx2.

    abstract:BACKGROUND:Some microRNAs (miRNAs) are involved in osteogenic differentiation. In recent years, increasing evidences have revealed that exosomes contain specific miRNAs. However, the effect and mechanism of miR-23a-5p-containing exosomes in osteoblast remain largely unclear. METHODS:We extracted exosomes from RANKL-in...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2019.109504

    authors: Yang JX,Xie P,Li YS,Wen T,Yang XC

    更新日期:2020-06-01 00:00:00

  • Downstream signalling events regulated by phosphatidylinositol 3-kinase activity.

    abstract::The phosphatidylinositol (PI) 3-kinase family of enzymes is now known to be regulated by several different upstream pathways in response to virtually all growth factors and cytokines. In the past few years, the phosphoinositides phosphorylated at the 3-OH position of the inositol ring have been shown to be lipid secon...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/s0898-6568(97)00129-0

    authors: Duronio V,Scheid MP,Ettinger S

    更新日期:1998-04-01 00:00:00

  • Activation of phosphoinositide 3-kinase in response to inflammation and nitric oxide leads to the up-regulation of cyclooxygenase-2 expression and subsequent cell proliferation in mesangial cells.

    abstract::In this study, we showed that nitric oxide (NO) donors induced the mesangial cell proliferation and cyclooxygenase-2 (COX-2) protein expression in murine mesangial cells. An inflammatory condition [lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma)] could also induce cell proliferation and significantly enhanc...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2004.11.015

    authors: Sheu ML,Chao KF,Sung YJ,Lin WW,Lin-Shiau SY,Liu SH

    更新日期:2005-08-01 00:00:00

  • Adenosine A1 receptors inhibit both adenylate cyclase activity and TRH-activated Ca2+ channels by a pertussis toxin-sensitive mechanism in GH3 cells.

    abstract::The present study has examined the effects of adenosine A1 receptors on second messenger processes in GH3 cells. A1 receptors are present which are shown to inhibit adenylate cyclase in a GTP-requiring manner. Hormone (VIP) stimulation is also absolutely required for the observation of inhibition. Adenosine A1 recepto...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(89)90023-5

    authors: Cooper DM,Caldwell KK,Boyajian CL,Petcoff DW,Schlegel W

    更新日期:1989-01-01 00:00:00

  • ZIP kinase, a key regulator of myosin protein phosphatase 1.

    abstract::Two major physiological roles have been defined for zipper interacting protein kinase (ZIPK), regulation of apoptosis in non-muscle cells and regulation of Ca(2+) sensitization in smooth muscle. Although much attention has focused on the role of ZIPK in the regulation of apoptotic events, its roles in smooth muscle ar...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2005.05.008

    authors: Haystead TA

    更新日期:2005-11-01 00:00:00

  • The stoichiometry of expression of protein components of the stimulatory adenylyl cyclase cascade and the regulation of information transfer.

    abstract::Quantitative analysis of the proteins which compromise the stimulatory arm of the adenylyl cyclase cascade indicate that the adenylyl cyclase catalytic component is usually the least highly expressed. The effects on both potency of agonist ligands and maximal output resulting from targetted alterations in expression l...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/0898-6568(95)02034-9

    authors: Milligan G

    更新日期:1996-02-01 00:00:00

  • TMEPAI/PMEPA1 inhibits Wnt signaling by regulating β-catenin stability and nuclear accumulation in triple negative breast cancer cells.

    abstract::Transmembrane prostate androgen-induced protein (TMEPAI) is a type I transmembrane protein induced by several intracellular signaling pathways such as androgen, TGF-β, EGF, and Wnt signaling. It has been reported that TMEPAI functions to suppress TGF-β and androgen signaling but here, we report a novel function of TME...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2019.03.016

    authors: Amalia R,Abdelaziz M,Puteri MU,Hwang J,Anwar F,Watanabe Y,Kato M

    更新日期:2019-07-01 00:00:00

  • The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling.

    abstract::Previous reports have suggested that the two mitogen-activated protein kinases (MAPKs) in Dictyostelium discoideum, ERK1 and ERK2, can be directly activated in response to external cAMP even though these MAPKs play different roles in the developmental life cycle. To better characterize MAPK regulation, the levels of p...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.10.009

    authors: Schwebs DJ,Hadwiger JA

    更新日期:2015-01-01 00:00:00

  • Spatial regulation of signaling by the coordinated action of the protein tyrosine kinases MET and FER.

    abstract::A critical aspect of understanding the regulation of signal transduction is not only to identify the protein-protein interactions that govern assembly of signaling pathways, but also to understand how those pathways are regulated in time and space. In this report, we have applied both gain-of-function and loss-of-func...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.06.006

    authors: Zhang J,Wang Z,Zhang S,Chen Y,Xiong X,Li X,Tonks NK,Fan G

    更新日期:2018-10-01 00:00:00

  • Deregulation of Tpl2 and NF-kappaB signaling and induction of macrophage apoptosis by the anti-depressant drug lithium.

    abstract::Lithium is an anti-depressant drug that also possesses immunomodulatory functions. The anti-inflammatory effect of lithium is thought to involve activation of the transcription factor CREB, although the underlying mechanism is incompletely understood. We show here that in macrophages lithium stimulates Tpl2, a MAP kin...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.12.010

    authors: Zhang M,Jin W,Zhou X,Yu J,Lee AJ,Sun SC

    更新日期:2009-04-01 00:00:00

  • Ephedrine induced thioredoxin-1 expression through β-adrenergic receptor/cyclic AMP/protein kinase A/dopamine- and cyclic AMP-regulated phosphoprotein signaling pathway.

    abstract::Ephedrine (Eph) is one of alkaloids that has been isolated from the ancient herb ephedra (ma huang) and is used as the treatment of asthma, hypotension and fatigue. However, its molecular mechanism remains unknown. Thioredoxin-1 (Trx-1) is a redox regulating protein, which has various biological activities, including ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.02.007

    authors: Jia JJ,Zeng XS,Li Y,Ma S,Bai J

    更新日期:2013-05-01 00:00:00

  • Human colon carcinoma cell-line HCT116 transfected by antisense cDNA as a tool to study the Ku86 involvement in cell proliferation.

    abstract::In this work, we used colon carcinoma cell-line HCT116 to study the involvement of the 86-kDa subunit (Ku86) of DNA-protein kinase (DNA-PK) in human tumoural cell proliferation. We transfected these cells with a 639-bp cDNA encoding a Ku86 portion inserted into pcDNA3.1 vector in the antisense orientation. After selec...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(00)00126-1

    authors: Sadji Z,Le Romancer M,Lewin MJ,Reyl-Desmars F

    更新日期:2000-12-01 00:00:00

  • A cell surface inactive mutant of the human lutropin receptor (hLHR) attenuates signaling of wild-type or constitutively active receptors via heterodimerization.

    abstract::The D405N and Y546F mutations of the human lutropin receptor (hLHR) have previously been shown to partially attenuate hCG-stimulated cAMP synthesis despite normal cell surface expression and hCG binding affinity (Min, L. and Ascoli, M. Mol. Endocrinol. 14:1797-1810, 2000). We now show that these mutations each stabili...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.07.003

    authors: Zhang M,Feng X,Guan R,Hébert TE,Segaloff DL

    更新日期:2009-11-01 00:00:00

  • DDX17 (P72), a Sox2 binding partner, promotes stem-like features conferred by Sox2 in a small cell population in estrogen receptor-positive breast cancer.

    abstract::We have previously demonstrated the existence of two phenotypically distinct cell subsets in estrogen receptor (ER)-positive breast cancer (BC) based on their differential response to a Sox2 reporter (SRR2), with reporter responsive (RR) cells being more tumorigenic and stem-like than reporter unresponsive (RU) cells....

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2015.11.004

    authors: Alqahtani H,Gopal K,Gupta N,Jung K,Alshareef A,Ye X,Wu F,Li L,Lai R

    更新日期:2016-02-01 00:00:00

  • Lysophospholipids and chemokines activate distinct signal transduction pathways in T helper 1 and T helper 2 cells.

    abstract::We demonstrate the expression of S1P(1,3,4,5) the receptors for sphingosine 1-phosphate (S1P), and LPA(1,2,3) the receptors for lysophosphatidic acid (LPA) in T helper 1 (Th1) and T helper 2 (Th2) cells. S1P and LPA induce the chemotaxis of Th1 and Th2 cells, an activity that is resistant to pertussis toxin (PTX) pret...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2004.02.001

    authors: Wang L,Knudsen E,Jin Y,Gessani S,Maghazachi AA

    更新日期:2004-09-01 00:00:00