Abstract:
:Balanced reciprocal translocation carriers are usually phenotypically normal but are at an increased risk of infertility, recurrent miscarriage or having affected children. Preimplantation genetic testing on chromosomal structural rearrangement (PGT-SR) offers a way to screen against unbalanced embryos. Here, we demonstrated a new method to distinguish carrier from noncarrier embryos. Translocation breakpoints were first delineated by nanopore sequencing followed by polymerase chain reaction (PCR) across breakpoints. High-resolution breakpoint mapping was successful in all (9/9) balanced reciprocal translocation carriers. Retrospective analysis of their embryo biopsies with breakpoint PCR showed 100% concordant results with PGT-SR on trophectoderm biopsies (40/40) and 53% concordance on blastomere biopsies (8/15). The low concordant rate in blastomeres was due to failure in the amplification of derivative chromosomes involving large deletions. Breakpoint PCR also showed 100% concordant results with prenatal/postnatal outcomes on 5 pregnancies, indicating that our new method can accurately distinguish carrier from noncarrier embryos.
journal_name
Genomicsjournal_title
Genomicsauthors
Chow JFC,Cheng HHY,Lau EYL,Yeung WSB,Ng EHYdoi
10.1016/j.ygeno.2019.04.001subject
Has Abstractpub_date
2020-01-01 00:00:00pages
494-500issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(18)30617-7journal_volume
112pub_type
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