Abstract:
:MicroRNAs (miRNAs) are a group of RNAs that play important roles in regulating gene expression and protein translation. In a previous study, we established an oligonucleotide microarray platform to detect miRNA expression. Because it contained only hundreds of probes, data normalization was difficult. In this study, the microarray data for eight miRNAs extracted from inflamed rat dorsal root ganglion (DRG) tissue were normalized using 15 methods and compared with the results of real-time polymerase chain reaction. It was found that the miRNA microarray data normalized by the print-tip loess method were the most consistent with results from real-time polymerase chain reaction. Moreover, the same pattern was also observed in 14 different types of rat tissue. This study compares a variety of normalization methods and will be helpful in the preprocessing of miRNA microarray data.
journal_name
Genomicsjournal_title
Genomicsauthors
Hua YJ,Tu K,Tang ZY,Li YX,Xiao HSdoi
10.1016/j.ygeno.2008.04.002subject
Has Abstractpub_date
2008-08-01 00:00:00pages
122-8issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(08)00083-9journal_volume
92pub_type
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