Abstract:
:The emergence of neuronal diversity during development of the nervous system relies on dynamic changes in the epigenetic landscape of neural stem cells and their progeny. Targeted DamID (TaDa) is proving invaluable in identifying the genome-wide binding sites of chromatin-associated proteins in vivo, without fixation, cell isolation, or immunoprecipitation. The simplicity and efficiency of the technique have led to an ever-expanding TaDa toolbox. These tools enable profiling of gene expression and chromatin accessibility, as well as the identification of the genome-wide binding sites of chromatin complexes, transcription factors and RNAs. Here, we review these new developments, with particular emphasis on the use of TaDa in studying neuronal specification.
journal_name
Curr Opin Neurobioljournal_title
Current opinion in neurobiologyauthors
van den Ameele J,Krautz R,Brand AHdoi
10.1016/j.conb.2019.01.021subject
Has Abstractpub_date
2019-06-01 00:00:00pages
160-166eissn
0959-4388issn
1873-6882pii
S0959-4388(19)30022-4journal_volume
56pub_type
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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journal_title:Current opinion in neurobiology
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