Abstract:
BACKGROUND:Cancer, one of the leading causes of death worldwide, develops when the normal balance between mitosis and apoptosis is disrupted. The subsequently increased proliferation rate or decreased apoptosis rate of cells leads to uncontrolled cellular growth. Thus, the current aim of cancer research is to increase the apoptosis rate in tumor cells-while limiting the concurrent death of healthy cells-and to induce controlled apoptosis in abnormal cells. Staurosporine is a very potent inducer of apoptosis because it inhibits many different kinases. So far, many different kinase pathways of staurosporine-induced apoptosis have been discussed for various tumor entities. AIMS:To identify the effect of staurosporine in pancreatic and colorectal carcinoma cells and its apoptosis-inducing signaling pathway. METHODS:The apoptosis rate in pancreatic and colorectal carcinoma cells was analyzed by annexin V staining after staurosporine administration. Staurosporine stimulation and its effects on the expression of Bcl2, BAX, Bad, caspase-8, and caspase-9 were investigated with immunoblot. RESULTS:Staurosporine significantly increased apoptosis in pancreatic carcinoma cells. Western blot analysis showed activation of caspase-9 in PaTu 8988t and Panc-1 cells with 1 µM staurosporine. In addition, expression of Bcl2 and Bad was decreased in PaTu 8988t cells. In colorectal carcinoma cells SW 480, staurosporine stimulation did not induce apoptosis. CONCLUSION:Modern therapeutic strategies for tumor diseases target the efficient modulation of specific signaling and transcription pathways. In this respect, the therapeutic potential of protein kinase inhibitors has been repeatedly discussed. Our study showed that staurosporine induces apoptosis in pancreatic carcinoma cells via the intrinsic signaling pathway. Thus, staurosporine is a suitable positive control for in vitro apoptosis tests for the pancreatic cancer cell lines PaTu 8988t and Panc-1. Further clinical studies should analyze the impact of this finding on cancer treatment.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Malsy M,Bitzinger D,Graf B,Bundscherer Adoi
10.1186/s40001-019-0365-xsubject
Has Abstractpub_date
2019-01-28 00:00:00pages
5issue
1eissn
0949-2321issn
2047-783Xpii
10.1186/s40001-019-0365-xjournal_volume
24pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to examine the influence of immuno modulating agents like bromelain and trypsin (e.g. Wobenzym on granulocyte and monocyte functions in healthy volunteers and patients with disorders of the humoral immuno system X-linked agammaglobulinaemia (XLA) and common variable immuno deficiency...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2001-05-29 00:00:00
abstract:SUBJECT:Recent studies have shown that smoking and drinking are associated with poorer outcomes in patients with cardiomyopathy. The purpose of this study was to determine all-cause mortality in dilated cardiomyopathy (DCM) associated with smoking and drinking. METHODS:An observational cohort study was undertaken in D...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-015-0171-z
更新日期:2015-09-17 00:00:00
abstract:OBJECTIVE:Evaluating the effects of prophylactic administration of IgM-enriched immunoglobulins (IVIG) on immunological- and clinical parameters in cardiac surgical patients. PATIENTS AND METHODS:41 patients were randomized to receive either an IgM-enriched immunoglobulin (Pentaglobin(R)) preparation (1,300 ml immunog...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-12-17 00:00:00
abstract:BACKGROUND:In intestinal epithelium, tyrosine kinase receptor Ephrin B3 (Eph B3) maintains the architecture of the crypt-villus axis by repulsive interaction with its ligand ephrin-B1. While loss of Eph B3 is linked to colorectal cancer initiation, overexpression of Eph B3 in cancer cell lines inhibits growth and induc...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-17-10
更新日期:2012-05-14 00:00:00
abstract::We investigated the hypothesis that religious commitment could help counter general affective distress, accompanying depressive symptoms, in older age. A total of 34 older adults, all catholic believers, completed self-reported questionnaires on the presence of depressive symptoms, religiosity, health, worry, and the ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-16-9-401
更新日期:2011-09-12 00:00:00
abstract:AIMS:This study assessed the prevalence of substance use among Iranian patients with cardiovascular disease who were admitted in different cardiac wards at Shiraz general hospitals. DESIGN:Cross-sectional survey using structured interview and also using DSM-IV criteria for substance dependency. SETTING:General hospit...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2002-02-21 00:00:00
abstract:BACKGROUND:Prostate cancer (PCa) originating from the prostate base may intrude into the urinary bladder and may be misdiagnosed as bladder cancer. In this retrospective study, we reviewed the clinic data on PCa cases which were initially misdiagnosed as bladder cancer in order to identify diagnostic methods that would...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-36
更新日期:2013-10-03 00:00:00
abstract::The current report describes the experience from the Frankfurt AIDS Cohort Study with patients suffering from renal failure. The clinical data of 4993 HIV-infected patients between 1983 and 1998 were analyzed retrospectively. Patients were seen at least twice a year and clinical features, routine laboratory results, i...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2000-05-23 00:00:00
abstract:BACKGROUND:Exacerbations of chronic obstructive pulmonary disease (COPD) are sporadic, acute worsening of symptoms. Identifying predictors of exacerbation frequency may facilitate medical interventions that reduce exacerbation frequency and severity. The objective of this study was to determine predictors of exacerbati...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-19-18
更新日期:2014-04-08 00:00:00
abstract:BACKGROUND:The Orthogonal Polarization Spectral (OPS) imaging technique has been introduced for in vivo assessment of microcirculation in humans. Unclear remains, whether the new technique would allow for accurate investigations of the colon microcirculation with respect to the unique conditions of the microvasculature...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2001-12-17 00:00:00
abstract:BACKGROUND:Chondral or osteochondral lesions, post-traumatic contracture and loose bodies of the elbow are often associated with chronic pain, stiffness, repetitive swelling and joint blockages. Therefore, arthroscopy of the elbow is often used in the elderly for the treatment of osteochondral defects or arthrolysis. T...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0338-5
更新日期:2018-09-15 00:00:00
abstract:BACKGROUND:It is hitherto still questionable which of the physical properties of the bile fluid influence the outcome of extracorporeal shock wave lithotripsy (ESWL). METHODS:In this study the influence of the density and viscosity of bile simulating fluid on ESWL was tested in vitro by applying CsCl solutions of dens...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-01-19 00:00:00
abstract::Here we illustrate a 74-year-old patient presenting with clinical signs of slowly progressive motor neuron disease. After electromyography demonstrated myopathic changes, a skeletal muscle biopsy was performed showing histological signs of granulomatous myositis. In extensive diagnostic workup underlying primary granu...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2008-07-28 00:00:00
abstract:OBJECTIVE:This study aims to discuss the correlation between serum inflammatory cytokines and neurogenic pulmonary edema (NPE) in children with severe hand-foot-mouth disease (HFMD). METHODS:A total of 89 patients with severe HFMD were enrolled into this study. These patients were divided into two groups, according to...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0313-1
更新日期:2018-05-03 00:00:00
abstract::The influence of heparin on Polymorphonuclear (PMN s) leukocytes was investigated using a new whole-blood cytofluorometric method (patent granted for the test with the number P 4334935.8-41) with Candida albicans and Staphylococcus aureus as test microorganisms. After comparing the effect of equal volumes of two widel...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-12-31 00:00:00
abstract::Exposure to high concentrations of oxygen is known to induce changes in lung function through effects on several pulmonary cell types, including alveolar macrophages (AM). In this study, we studied the in vitro effects of hyperoxia on the release of proinflammatory cytokines and the expression of surface receptors in ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-04-21 00:00:00
abstract:BACKGROUND:Data on lung volumes and changes in flow-volume spirometry at high altitude are few and do not provide comprehensive assessment of the occurring changes. This study characterizes alterations of the forced expiratory flow-volume curve (FEFV-curve) and lung volumes at increasing altitude. METHODS:FEFV-curve a...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-11-16 00:00:00
abstract::Bacteremia due to Capnocytophaga sputigena occurred in a 4-year and 9-month-old Japanese girl patient with acute erythroblastic leukemia in Shinshu University Hospital, Japan. On her admission to the hospital, she had a temperature of 38.2 degrees C with canker sore. Prior to the commencement of chemotherapy, peripher...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2008-03-31 00:00:00
abstract:BACKGROUND:Multiple severe trauma frequently leads to massive dysbalances of the human immune system. This phenomenon is known as "Systemic Inflammatory Response Syndrome (SIRS)". SIRS is connected to multiple organ failure and thereby entails higher morbidity and mortality in trauma patients. Pro- and anti-inflammator...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-7-284
更新日期:2009-07-22 00:00:00
abstract:BACKGROUND:Focal colorectal uptake in (18)FDG-PET/CT may be associated with a malignancy and can be quantified. This provides the basis for an automatic trigger threshold above which cases are flagged for colonoscopic evaluation and below which for individual assessment. PURPOSE:To determine the lowest maximum standar...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-016-0195-z
更新日期:2016-01-09 00:00:00
abstract::Determination of fecal excretion of the serum proteinase inhibitor alpha(1)-antitrypsin (AAT) is established for quantification of intestinal protein loss. It was demonstrated to be increased both in quiescent and in active inflammatory bowel diseases (IBD, Crohn's disease and ulcerative colitis). The (patho)physiolog...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1998-02-21 00:00:00
abstract::Plexiform neurofibroma (PN) is a rare benign tumor and a special subtype of neurofibromatosis type 1 (NF1). Though the incidence is low, giant PN of the craniomaxillofacial region could result in severe hemifacial hypertrophy which is known as a typical manifestation of NF1 in young children. Here, we retrospectively ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-2-84
更新日期:2010-02-26 00:00:00
abstract::Severe pulmonary hypertension is a lethal group of disorders which preferentially afflicts women. It appears that in recent years the patient profile has shifted towards older, obese, and postmenopausal women, suggesting that endocrine factors may be important. Several studies have revealed an increased prevalence of ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-10-433
更新日期:2009-09-28 00:00:00
abstract:BACKGROUND:Prosthetic joint infection (PJI) is a serious complication of orthopedic implant surgery. Treatment often includes the use of an antibiotic-loaded Polymethyl methacrylate (PMMA) bone cement spacer. Several antibiotics are commonly used for the preparation of these spacers, but due to the increasing number of...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-020-00428-z
更新日期:2020-08-18 00:00:00
abstract::In the absence of thromboprophylaxis, venous thromboembolism (VTE) affects about 50 to 80% of the patients after total hip replacement (THR), total knee replacement (TKR), or hip fracture surgery. Since stratification of patients in those who will become symptomatic and those who will not, is not possible, primary hig...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-30 00:00:00
abstract:BACKGROUND:Several studies have been performed in tumour patients to analyse the immunological response to mistletoe extracts. Considering the fact that these extracts are given subcutaneously in most instances, the kind of application resembles a typical immunization schedule. We therefore wanted to see how those extr...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-04-30 00:00:00
abstract:BACKGROUND:Patients with human immunodeficiency virus (HIV) infection have an increased risk of cardiovascular diseases. Previous publications described pericardial effusion as one of the most common HIV-associated cardiac affiliations. The aim of the current study was to investigate if pericardial effusion still has a...
journal_title:European journal of medical research
pub_type: 杂志文章,多中心研究
doi:10.1186/2047-783x-16-11-480
更新日期:2011-11-10 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the efficacy and safety of composite extracts in reducing weight, as the main outcome measure. Secondary measures of the study were body composition change. DESIGN:Randomised, double blind, placebo-controlled clinical trial. SETTING:Tertiary university clinic. SUBJECTS...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2006-08-30 00:00:00
abstract:BACKGROUND:Systemic treatment of head and neck squamous cell carcinoma (HNSCC) includes a variety of antineoplastic drugs. However, drug-resistance interferes with the effectiveness of chemotherapy. Preclinical testing models are needed in order to develop approaches to overcome chemoresistance. - METHODS:Ten human ce...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-8-337
更新日期:2010-08-20 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the association between leisure-time physical activity (LPA) and the risk of metabolic syndrome (MS). METHODS:Prospective cohort studies of the association between LPA and the risk of MS were retrieved from the PubMed and Embase databases up to 12 August 2013. The sta...
journal_title:European journal of medical research
pub_type: 杂志文章,meta分析,收录出版
doi:10.1186/2047-783X-19-22
更新日期:2014-04-23 00:00:00