Abstract:
BACKGROUND:Prosthetic joint infection (PJI) is a serious complication of orthopedic implant surgery. Treatment often includes the use of an antibiotic-loaded Polymethyl methacrylate (PMMA) bone cement spacer. Several antibiotics are commonly used for the preparation of these spacers, but due to the increasing number of infections with resistant Gram-negative bacteria, there is a need for the use of carbapenem antibiotics such as meropenem and imipenem as drugs of last resort. Unfortunately, the reaction heat generated during the preparation of the bone cement can be a major problem for the stability of these antibiotics. In the present study, the stability of meropenem and imipenem was tested before and after the admixture to PMMA bone cements. METHODS:High-performance liquid chromatography with ion-pairing reversed-phase separation and spectrophotometric detection was used for analysis. Stability tests with meropenem and imipenem were performed with antibiotics in solution and solid form at different temperatures (37 °C, 45 °C, 60 °C, 90 °C) and times (30 min, 60 min, 120 min). To test the stability of both antibiotics in PMMA after exposure to the reaction heat during polymerization, three different bone cements were used to generate specimens that contained defined amounts of antibiotics. Reaction heat was measured. The form bodies were mechanically crushed and aliquots were dissolved in ethyl acetate. Samples were prepared for HPLC DAD analysis. RESULTS:Meropenem and imipenem showed the highest degradation levels after heat stressed in solution, with maximum levels of 75% and 95%, respectively. In solid form, degradation levels decreased dramatically for meropenem (5%) and imipenem (13%). Stability tests of both carbapenems in bone cement showed that they remained largely stable during PMMA polymerization, with retrieved amounts of about 70% in Palacos® R and Copal® G+V, and between 80 and 90% in Copal® spacem. CONCLUSIONS:In contrast to the results of meropenem and imipenem in solution, both antibiotics remain stable in solid form and mostly stable in the cement after PMMA polymerization. The low degradation levels of both antibiotics after exposure to temperatures > 100 °C allow the conclusion that they can potentially be used for an application in PMMA cements.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Schmid M,Steiner O,Fasshold L,Goessler W,Holl AM,Kühn KDdoi
10.1186/s40001-020-00428-zsubject
Has Abstractpub_date
2020-08-18 00:00:00pages
34issue
1eissn
0949-2321issn
2047-783Xpii
10.1186/s40001-020-00428-zjournal_volume
25pub_type
杂志文章abstract:BACKGROUND:International guidelines for the treatment of HIV-1 infected children recommend efavirenz plus nucleoside reverse transcriptase inhibitor combination therapy for first line therapy. Until now little is known about the steady state pharmacokinetics of efavirenz in children. METHODS:11 HIV-1 infected children...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-09-29 00:00:00
abstract:OBJECTIVE:Pulmonary cavitation is the classic hallmark of pulmonary tuberculosis (PTB) and is the site of very high mycobacterial burden associated with antimycobacterial drug resistance and treatment failure. The objective of this study was to investigate the relationship between re-treatment PTB and initial pulmonary...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-016-0214-0
更新日期:2016-05-06 00:00:00
abstract::Plexiform neurofibroma (PN) is a rare benign tumor and a special subtype of neurofibromatosis type 1 (NF1). Though the incidence is low, giant PN of the craniomaxillofacial region could result in severe hemifacial hypertrophy which is known as a typical manifestation of NF1 in young children. Here, we retrospectively ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-2-84
更新日期:2010-02-26 00:00:00
abstract::More than one quarter of total daily calories are normally provided by fatty acids which contain at least one double bond. The usual configuration of these double bonds is the cis configuration. Trans fatty acids (TFA) are formed in technological and microbiological processes by isomerization of cis double bonds to tr...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2003-08-20 00:00:00
abstract::Photoplethysmography enables non-invasive investigation of the volume pulse in the microvasculature of patients. We previously have shown that time-discrete analysis enables identification of highly reproducible characteristics of the volume pulse in absolute values. The method would be of particular interest, if the ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1998-05-12 00:00:00
abstract::In Germany during the past years about 200-250 HIV infected pregnant women delivered a baby per year, a number that is currently increasing. To determine the HIV-status early in pregnancy voluntary HIV-testing of all pregnant women is recommended in Germany and Austria as part of prenatal care. In those cases, where H...
journal_title:European journal of medical research
pub_type: 共识发展会议,杂志文章,评审
doi:
更新日期:2004-06-30 00:00:00
abstract::Gadolinium chelates are widely used in magnetic resonance imaging as contrast medium in patients with nephropathy. However, only few studies have investigated the effect of gadolinium on serum creatinine concentration and estimated GFR as surrogate markers of renal function. This study was performed to evaluate the ef...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-04-20 00:00:00
abstract::Intercellular adhesion molecule-1 (ICAM-1) plays an important role in inflammatory diseases. It is believed that its soluble form (sICAM-1) might be a serum parameter of inflammatory activity with possible relevance in granulomatous disorders. To evaluate this role we measured sICAM-1 by ELISA in serum and shedding of...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1998-06-17 00:00:00
abstract::The 825T-allele of the C825T polymorphism in GNB3, the gene for the G protein beta3 subunit, has been reported to be associated with essential hypertension and obesity. Expression of Gbeta3s, the gene product of GNB3 associated with the GNB3 825T-allele, causes increased signal transduction which may contribute to pat...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2003-03-27 00:00:00
abstract::Among the 763 patients with ulcers and stomach cancer, including the control group of 586 blood donors, the search for a relationship between the spread of infection and the main blood groups was defined by the appearance of IgG against H. pylori in blood plasma (the ELISA test). The statistical analysis, however, did...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-03-19 00:00:00
abstract:BACKGROUND:Cancer, one of the leading causes of death worldwide, develops when the normal balance between mitosis and apoptosis is disrupted. The subsequently increased proliferation rate or decreased apoptosis rate of cells leads to uncontrolled cellular growth. Thus, the current aim of cancer research is to increase ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-019-0365-x
更新日期:2019-01-28 00:00:00
abstract::Though AIDS-related morbidity and mortality are generally decreasing as a result of highly active antiretroviral therapy (HAART) and prevention of opportunistic infections, dual infection with HCV and HIV leads to an acceleration in the natural course of chronic hepatitis C (cHC) and worsening of associated liver dise...
journal_title:European journal of medical research
pub_type:
doi:
更新日期:2002-01-29 00:00:00
abstract:BACKGROUND:This study investigates the effects of human bone marrow-derived mesenchymal stem cell (hMSC) on migration and proliferation ability of hepatocellular carcinoma (HCC) with high- and low-metastatic potential. METHODS:The hMSC and transforming growth factor-β1 (TGFβ-1) gene infected hMSC were co-cultured with...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-015-0144-2
更新日期:2015-05-24 00:00:00
abstract:BACKGROUND:Sepsis leads to severe inflammatory and cardiac dysfunction. This study aimed to explore the clinical value of miR-495 in sepsis, as well as its role in sepsis-induced inflammation and cardiac dysfunction. METHODS:105 sepsis patients were recruited; receiver operating characteristic (ROC) curve was plotted ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-019-0396-3
更新日期:2019-11-26 00:00:00
abstract:INTRODUCTION:Azathioprine has variable efficacy in inflammatory bowel disease. Previous studies suggested that either neutropenia, an increase in the mean corpuscular volume, the assessment of thiopurine methyl-transferase activity or erythrocyte 6-thioguanine values might predict the treatment response. However, due t...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章
doi:
更新日期:2005-12-07 00:00:00
abstract:BACKGROUND:Tick-borne encephalitis (TBE) is a viral infection of the CNS with significant acute and long-term morbidity. Dysfunction of the autonomic nervous system may be a potentially harmful complication of TBE. MATERIAL AND METHODS:In a retrospective case series, 5 patients with acute TBE were evaluated for clinic...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-06-30 00:00:00
abstract:OBJECTIVE:This study aims to discuss the correlation between serum inflammatory cytokines and neurogenic pulmonary edema (NPE) in children with severe hand-foot-mouth disease (HFMD). METHODS:A total of 89 patients with severe HFMD were enrolled into this study. These patients were divided into two groups, according to...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0313-1
更新日期:2018-05-03 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the efficacy and safety of composite extracts in reducing weight, as the main outcome measure. Secondary measures of the study were body composition change. DESIGN:Randomised, double blind, placebo-controlled clinical trial. SETTING:Tertiary university clinic. SUBJECTS...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2006-08-30 00:00:00
abstract:BACKGROUND:The pathogenesis of central retinal artery occlusions (CRAO) varies, depending upon the underlying disease. An embolic origin of an occlusion often occurs. PATIENT AND METHODS:A 50-year-old man with an occlusion of the left internal carotid artery was examined because of a left central retinal artery occlus...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-04-19 00:00:00
abstract::The European Directive 98/79/EC on in vitro diagnostic medical devices (IVD) stipulates the marketing and post market surveillance of IVD in the European Economic Area. In cases of issues and field corrective actions, the manufacturers have to inform the responsible Competent Authorities (CA). In Germany, the Federal ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-s4-216
更新日期:2009-12-07 00:00:00
abstract:OBJECTIVE:Due to the side effects of antiretroviral therapy and long term survival there is an increasing concern of an elevated rate of cardiovascular diseases in HIV-infected patients. The present study analysed the cardiovascular risk profile and the probability of cardiovascular events in HIV-infected patients, due...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2004-02-27 00:00:00
abstract::The gum resin of Boswellia serrata, known in Indian Ayurvedic system of medicine as Salai guggal, contains boswellic acids, which have been shown to inhibit leukotriene biosynthesis. In a double-blind, placebo-controlled study forty patients, 23 males and 17 females in the age range of 18 - 75 years having mean durati...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1998-11-17 00:00:00
abstract::CMV infection is common in pediatric HIV-1 infected patients. We present a case report, to our knowledge the first pediatric patient, in which Addison-disease due to CMV was suspected during lifetime. We want to point out the importance of routine clinical and laboratory follow up once a HIV-1 infected child is shown ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-06-20 00:00:00
abstract:BACKGROUND:Despite extensive research, the underlying pathological mechanisms of osteoporosis are not completely understood. Recent studies have indicated a distinct role for the IFN-β/STAT1 pathway in bone metabolism. An inhibitory effect of IFN-β on osteoclastogenesis has been detected and STAT1/2 has been shown to i...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-014-0074-4
更新日期:2015-01-07 00:00:00
abstract:BACKGROUND:Multiple severe trauma frequently leads to massive dysbalances of the human immune system. This phenomenon is known as "Systemic Inflammatory Response Syndrome (SIRS)". SIRS is connected to multiple organ failure and thereby entails higher morbidity and mortality in trauma patients. Pro- and anti-inflammator...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-7-284
更新日期:2009-07-22 00:00:00
abstract:BACKGROUND:Melasma is a common hypermelanosis of the face. The use of a classical Q-switched ruby laser (QSRL) to treat melasma is discussed controversially and is associated with frequent adverse effects, such as hyper- or hypopigmentation. Recently a fractional-mode (FRx) QSRL was developed to minimize the adverse ef...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-43
更新日期:2013-11-14 00:00:00
abstract::Human Immunodeficiency virus can affect the peripheral (PNS) and central nervous system (CNS). In the ART / HAART era especially the CNS is said to be a virus reservoir so that neurologists have to be more and more integrated in therapy planning. Therefore the present paper describes neurological indications for ART, ...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1999-11-22 00:00:00
abstract:OBJECTIVES:At workplaces, organic solvents are often used as mixtures. Nevertheless, there is limited knowledge of their acute effects on human central nervous system. Here we report the effects of a toluene-acetone mixture. METHODS:In a parallel design, subgroups of 12 healthy men each were exposed to a mixture conta...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-09-12 00:00:00
abstract:OBJECTIVE:The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. METHODS:Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultur...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-1-35
更新日期:2010-01-29 00:00:00
abstract::Oxytocin is one of the most potent uterotonic agents and is known to fluctuate throughout the menstrual cycle, showing an increase during sexual stimulation and arousal, with a peak during orgasm in women. To date, limited data are available on the effects of oxytocin on the regulation of uterine contractility and tra...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-04-28 00:00:00