Abstract:
:We analyzed the state of the genomic DNA of the papovavirus SV40 in human keratinocytes as viral-infected cells gradually acquired a transformed phenotype over time. Initially, the vast majority of the viral DNA is maintained either in a full-length supercoiled form or as truncated subgenomic fragments with little evidence of integration. However, analyses of clonal populations revealed great heterogeneity and instability of the viral DNA, and we were able to isolate one clonal subpopulation in which integrated forms of the virus appeared to predominate. Similarly, uncloned populations eventually ceased production of the "free" viral DNA after several years in culture and instead came to display tandemly repeated SV40 copies at a single host integration site. Interestingly, Bg1 II digestion of host DNA generated restriction fragments containing the integrated SV40 DNA, which were of differing sizes in cultures at the 144th vs the 163rd serial passage suggesting modification or rearrangement of sequences at or near the integration site. Host sequences flanking the integrated viral DNA at the 163rd serial passage have been isolated on restriction fragments generated by Eco RI, Bam HI, and Hpa II digestion. These analyses suggest that the integrated virus is linearized near the Bg1 I site and contains a large deletion in the SV40 early region at one of the viral-host junctions.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Naimski P,Steinberg MLdoi
10.1002/jcb.240290205subject
Has Abstractpub_date
1985-01-01 00:00:00pages
95-103issue
2eissn
0730-2312issn
1097-4644journal_volume
29pub_type
杂志文章abstract::The non-receptor tyrosine kinase c-Src is activated in many human cancer types, and induces deregulation of cadherin-based cell-cell contacts and actin cytoskeleton. Because ezrin, a protein which cross-links the plasma membrane with the actin cytoskeleton, is often over-expressed in human cancers, and participates in...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20033
更新日期:2004-05-01 00:00:00
abstract::Alkaline phosphatase (ALP) refers to a group of nonspecific phosphomonoesterases located primarily in cell plasma membrane. It has been described in different cell lines that ecto-ALP is directly or indirectly involved in the modulation of organic cation transport. We aimed to investigate, in Caco-2 cells, a putative ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.10306
更新日期:2002-01-01 00:00:00
abstract::Madin-Darby canine kidney (MDCK) cells were previously shown to have few or no plasma membrane insulin binding sites (Hofmann et al: J Biol Chem 258:11774, 1983]. Accordingly, neither insulin-stimulated incorporation of [14C]glucose into glycogen, nor insulin-induced uptake of radiolabeled alpha-aminoisobutyrate ([3H]...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240270409
更新日期:1985-01-01 00:00:00
abstract::The NAD-dependent deacetylase Sirtuin 1 (SIRT1) plays a vital role in leukemogenesis. Nicotinamide (NAM) is the principal NAD+ precursor and a noncompetitive inhibitor of SIRT1. In our study, we showed that NAM enhanced the sensitivity of chronic myeloid leukemia (CML) to doxorubicin (DOX) via SIRT1. We found that SIR...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.29303
更新日期:2020-01-01 00:00:00
abstract::Four detergents have been compared for identification of the Plasmodium knowlesi variant antigen on infected erythrocytes by immunoprecipitation analysis. Erythrocytes infected with late trophozoite and schizont forms of cloned asexual parasites were labeled by lactoperoxidase-catalyzed radioiodination and extracted e...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240240310
更新日期:1984-01-01 00:00:00
abstract::O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a post-translational modification, which is believed antagonises phosphorylation. We have studied the O-GlcNAc level during Xenopus oocyte meiotic resumption, taking advantage of the high synchrony of this model which is dependent upon a burst of phosphorylation...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20242
更新日期:2004-11-15 00:00:00
abstract::Neurological disorders, such as stroke, are triggered by a loss of neurons and glial cells. Ischemic stroke remains a substantial problem for industrialized countries. Over the previous few decades our understanding about the pathophysiology of stroke has enhanced, nevertheless, more awareness is required to advance t...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.28207
更新日期:2019-06-01 00:00:00
abstract::Membrane vesicles were prepared by incubation of human erythrocytes with dimyristoylphosphatidylcholine [3] and isolated by isopycnic centrifugation on Dextran density gradients. Protein analyses were carried out with crossed immunoelectrophoresis and dodecylsulfate polyacrylamide gel electrophoresis. The right-side-o...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240190208
更新日期:1982-01-01 00:00:00
abstract::Although activated macrophages destroy cancer cells more effectively than normal cells, the ability to escape activated macrophages is a characteristic of tumor cells. One of the mechanisms responsible for the specific killing of tumor cells by macrophages is the production of the cytokine tumor necrosis factor (TNF) ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.21163
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:Long noncoding RNAs (lncRNAs) regulate gene expression by acting with microRNAs (miRNAs) and indirectly interact with messenger RNA (mRNAs). However, the roles of specific lncRNA and its related competing endogenous RNAs (ceRNA) network in alopecia areata (AA) are not fully understood. METHODS:The blood lnc...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28365
更新日期:2019-06-01 00:00:00
abstract::The deficiency of the enzyme glutaryl-CoA dehydrogenase, known as glutaric acidemia type I (GA-I), leads to the accumulation of glutaric acid (GA) and glutarilcarnitine (C5DC) in the tissues and body fluids, unleashing important neurotoxic effects. l-carnitine (l-car) is recommended for the treatment of GA-I, aiming t...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27332
更新日期:2018-12-01 00:00:00
abstract::Avian influenza viruses (AIV) are very active in several parts of the globe and are the cause of huge economic loss for the poultry industry and also human fatalities. Three dimensional modeling was carried out for neuraminidase (NA) and hemagglutinin (HA) proteins of AIV. The C-score, estimated TM-Score, and estimate...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.29017
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVES:This study is aimed to explore the functions of long noncoding RNA (lncRNA) HEIH on the proliferation as well as metastasis of non-small cell lung cancer (NSCLC). METHODS:HEIH was shown to be increased in NSCLC by a microarray study on lncRNA profiling. We detected the expression of HEIH in different human ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27629
更新日期:2019-03-01 00:00:00
abstract::We report here the identification and characterization of a novel SR-related protein, referred to as SRrp37, based on its apparent molecular weight and subcellular location. SRrp37 was identified through a yeast two-hybrid screen during the course of searching for proteins interacting with pNO40, a ribosomal 60S core ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22255
更新日期:2009-09-01 00:00:00
abstract::Angiogenesis, the formation of new capillary blood vessels, occurs almost exclusively in the microcirculation. This process is controlled by the interaction between factors with positive and negative regulatory activity. In this study, we have compared the effect of two well described positive regulators, vascular end...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.1190
更新日期:2001-01-01 00:00:00
abstract::Several molecules extracted from natural products exhibit different biological activities, such as ion channel modulation, activation of signaling pathways, and anti-inflammatory or antitumor activity. In this study, we tested the antitumor ability of natural compounds extracted from the Raputia praetermissa plant. Am...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28238
更新日期:2019-06-01 00:00:00
abstract::The influence of concentration of simvastatin (SIM) on free radicals in A-2058 human melanoma malignum cells was studied. The proliferation assay for melanoma A-2058 cells with SIM in concentration range from 0.1 to 20 µM was performed. SIM in the concentrations of 0.1, 0.3, and 1 μM only slightly changed the growth o...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27540
更新日期:2018-09-11 00:00:00
abstract::The in vitro growth of primary erythroleukemia cells has been examined in the presence and absence of the hormone erythropoietin (EPO). Although these leukemic cells had previously been considered to be hormone-independent, addition of EPO was found to be essential for maximum growth in culture. Erythroid colonies tha...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240300404
更新日期:1986-01-01 00:00:00
abstract::A23187 stimulates the metabolism of endogenous as well as exogenous arachidonic acid (AA) and eicosapentaenolc acid (EPA) to their corresponding leukotrienes in human neutrophils. In contrast, conflicting results have been obtained concerning the effect of FMLP on the metabolism of these fatty acids. In the present st...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240400310
更新日期:1989-07-01 00:00:00
abstract::Pyrazinamide is an essential first-line antitubercular drug which plays pivotal role in tuberculosis treatment. It is a prodrug that requires amide hydrolysis by mycobacterial pyrazinamidase enzyme for conversion into pyrazinoic acid (POA). POA is known to target ribosomal protein S1 (RpsA), aspartate decarboxylase (P...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28013
更新日期:2018-11-02 00:00:00
abstract::In order to better understand the mechanism by which changes in the fatty acid composition of cellular lipids occur in leukemia cell lines induced to differentiate, the activity of the first enzyme of fatty acid biosynthesis, acetyl-CoA carboxylase (EC 6.4.1.2) was measured in HL-60 promyelocytic leukemia cells before...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240260203
更新日期:1984-01-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants whose effects on biological systems depend on the number of and the positions of the chlorine substitutions. In the present study we examined the estrogenicity of the fully ortho-substituted PCB, 2,2',6,6'-tetrachlorobiphenyl (2,2',6,6'-TeCB). ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Phospholipase C-gamma1 (PLCgamma1) plays a critical role in cell growth and proliferation by generating the second messengers, diacylglycerol and 1, 4, 5-inositol triphosphate. To investigate the roles of Src homology domain 2 and domain 3 of PLCgamma1 in PLCgamma1-mediated cell signaling, we characterized some protei...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20357
更新日期:2005-04-01 00:00:00
abstract::Alzheimer's disease, a neurodegenerative disorder continues to be an area of investigation by the international researchers' fraternity. Despite all the ongoing efforts, the effective set of promising cholinesterase inhibitors available in the market for patients' use is limited. Furthermore, the currently available d...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28461
更新日期:2019-02-11 00:00:00
abstract::Stimulation of osteoblast survival signals may be an important mechanism of regulating bone anabolism. Protein kinase B (PKB/Akt), a serine-threonine protein kinase, is a critical regulator of normal cell growth, cell cycle progression, and cell survival. In this study we have investigated the signaling pathways activ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:2001-03-26 00:00:00
abstract::The cellular basis of metastasis is poorly understood. An important step to understanding this process is to be able to visualize the routes by which cancer cells migrate from the primary tumor to various distant sites to eventually form metastasis. Our laboratory previously developed single-cell in vivo imaging using...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25391
更新日期:2016-04-01 00:00:00
abstract::Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs)...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25838
更新日期:2017-07-01 00:00:00
abstract:AIM:To detect the expression and identify the role of Ribosomal protein S15A (RPS15A) in human breast cancer (BC). METHODS:Immunohistochemistry (IHC) was carried out for detecting the levels of RPS15A protein. Quantitative PCR was used to evaluate the mRNA level of RPS15A in one normal breast and three BC cell lines. ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.29304
更新日期:2020-01-01 00:00:00
abstract::The mitochondrial antioxidant enzyme Mn-Superoxide Dismutase (Sod2) is essential for mammalian survival. I82T mutation in human Sod2 has been linked to a wide variety of diseases, including Alzheimer's and Parkinson's diseases as well as some types of cancers. Yeast wild-type (WT) Sod2 and the mutant Sod2 I91T, which ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25720
更新日期:2017-05-01 00:00:00
abstract::The comet assay is one of the most widely used approaches for detecting DNA damage; generally, it provides information on the cell population-averaged level of DNA damage. Here, we present an automatic technique for easy measurement of standard comet characteristics and an annotation of the cell cycle phase of each co...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25615
更新日期:2016-10-01 00:00:00