Abstract:
:Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs) in this system, we found that miR-126 is highly upregulated in hOBs following coculturing with HUVECs. In this study we performed miR-126 gain-of-function and loss-of-function experiments in hOBs followed by microarray analysis in order to identify targets of miR-126. The transcript cluster IDs were sieved by applying cut-off criteria and by selecting transcripts which were upregulated following miR-126 downregulation and vice versa. The calmodulin regulated spectrin associated protein 1 (CAMSAP1) mRNA was confirmed to be differentially regulated by miR-126. Using the luciferase reporter assay it was demonstrated that CAMSAP1 is directly targeted by miR-126. In this study, we show that miR-126 and CAMSAP1 directly interact in hOBs. This finding has potential implications for tissue engineering applications. J. Cell. Biochem. 118: 1756-1763, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Strassburg S,Nabar N,Lampert F,Goerke SM,Pfeifer D,Finkenzeller G,Stark GB,Simunovic Fdoi
10.1002/jcb.25838subject
Has Abstractpub_date
2017-07-01 00:00:00pages
1756-1763issue
7eissn
0730-2312issn
1097-4644journal_volume
118pub_type
杂志文章abstract::A new view of the cell nucleus is emerging based on the functional dynamics of nuclear architecture. The striking structural preservation of a variety of genomic processes on the nuclear matrix provides an important approach for correlating nuclear form and function. In situ labeling coupled with three-dimensional mic...
journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2000-09-18 00:00:00
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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