Abstract:
:Macromolecules are sulfated during the vegetative growth of Dictyostelium discoideum. A characterisation of the structures of sulfated oligosaccharides associated with these macromolecules indicates that the oligosaccharides are heterogeneous. Endoglycosidase and pronase digestion were used with gel-filtration chromatography to obtain two different oligosaccharide fractions and a glycopeptide fraction; these were further characterised by ion-exchange and lectin-affinity chromatography and by acid hydrolysis. The data indicate that up to 43% of the sulfate is associated with typical N-linked oligosaccharides, that up to 5% is associated with N-linked oligosaccharides that are either very large or extremely highly charged, and that the remaining sulfate is associated with oligosaccharides non-N-linked to protein. Each fraction was also shown to be heterogeneous at most other structural levels. Electrophoretic analyses following the endoglycosidase and pronase treatments indicated that all of the macromolecules are glycoproteins and suggested further that at least two of the oligosaccharide fractions are located on different groups of glycoproteins.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Davis SJdoi
10.1002/jcb.240380202subject
Has Abstractpub_date
1988-10-01 00:00:00pages
77-86issue
2eissn
0730-2312issn
1097-4644journal_volume
38pub_type
杂志文章abstract::Lung cancer is the leading cause of death among all cancers. Non-small cell lung cancer accounts for 80% of lung cancer with a 5-year survival rate of 16%. Notch pathway, especially Notch-1 is up-regulated in a subgroup of non-small cell lung cancer patients. Since Notch-1 signaling plays an important role in cell pro...
journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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更新日期:2013-07-01 00:00:00
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更新日期:2015-08-01 00:00:00
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journal_title:Journal of cellular biochemistry
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