Control of intestinal promoter activity of the cellular migratory regulator gene ELMO3 by CDX2 and SP1.

Abstract:

:An important aspect of the cellular differentiation in the intestine is the migration of epithelial cells from the crypt to the villus tip. As homeodomaine transcription factor CDX2 has been suggested to influence cell migration, we performed a genome-wide promoter analysis for CDX2 binding in the differentiated human intestinal cancer cell line Caco-2 in order to identify CDX2-regulated genes involved in cellular migration. The engulfment and cell motility 3 (ELMO3) gene was identified as a potential CDX2 target gene. ELMO3 is an essential upstream regulator of the GTP-binding protein RAC during cell migration. However, no information is available about the transcriptional regulation of the ELMO3 gene. The aim of this study was to investigate the potential role of CDX2 in the regulation of the ELMO3 promoter activity. Electrophoretic mobility shift assays showed that CDX2 bound to conserved CDX2 sequences and mutations of the CDX2-binding sites, significantly reduced the promoter activity. Reporter gene assays demonstrated that the region mediating ELMO3 basal transcriptional activity to be located between -270 and -31 bp. Sequence analysis revealed no typical TATA-box, but four GC-rich sequences. In vitro analyses (electrophoretic mobility shift assays and promoter analyses) demonstrate that the SP1-binding sites are likely to play an important role in regulating the ELMO3 promoter activity. Furthermore, we showed here that CDX2 and SP1 can activate the ELMO3 promoter. Taken together, the present study reports the first characterization of the ELMO3 promoter and suggests a significant role of CDX2 in the basal transcriptional regulation of the intestine-specific expression of ELMO3, possibly through interaction with SP1.

journal_name

J Cell Biochem

authors

Coskun M,Boyd M,Olsen J,Troelsen JT

doi

10.1002/jcb.22490

subject

Has Abstract

pub_date

2010-04-15 00:00:00

pages

1118-28

issue

6

eissn

0730-2312

issn

1097-4644

journal_volume

109

pub_type

杂志文章
  • The association of the placental CASPASE-3 gene polymorphisms and preeclampsia susceptibility and in-silico analysis.

    abstract::Preeclampsia is a pathologic complication of pregnancy, associated with increased apoptosis in the cytotrophoblasts as the main cause of this disorder. Caspase-3 is a key apoptosis-related enzyme that both mitochondrial and death receptor apoptotic pathways can activate. In this study, we aimed to investigate the effe...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26869

    authors: Teimoori B,Yazdi A,Rezaei M,Mohammadpour-Gharehbagh A,Jahantigh D,Salimi S

    更新日期:2018-08-01 00:00:00

  • Modulation of O-GlcNAc glycosylation during Xenopus oocyte maturation.

    abstract::O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a post-translational modification, which is believed antagonises phosphorylation. We have studied the O-GlcNAc level during Xenopus oocyte meiotic resumption, taking advantage of the high synchrony of this model which is dependent upon a burst of phosphorylation...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20242

    authors: Lefebvre T,Baert F,Bodart JF,Flament S,Michalski JC,Vilain JP

    更新日期:2004-11-15 00:00:00

  • Hepatocyte uptake of alpha 1-proteinase inhibitor-trypsin complexes in vitro: evidence for a shared uptake mechanism for proteinase complexes of alpha 1-proteinase inhibitor and antithrombin III.

    abstract::In vivo clearance studies have indicated that the clearance of proteinase complexes of the homologous serine proteinase inhibitors alpha 1-proteinase inhibitor and antithrombin III occurs via a specific and saturable pathway located on hepatocytes. In vitro hepatocyte-uptake studies with antithrombin III-proteinase co...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240250405

    authors: Fuchs HE,Michalopoulos GK,Pizzo SV

    更新日期:1984-01-01 00:00:00

  • Energy metabolism characterization of a novel cancer stem cell-like line 3AB-OS.

    abstract::Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutic target, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells, exhibit a reduced mitochondrial acti...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24671

    authors: Palorini R,Votta G,Balestrieri C,Monestiroli A,Olivieri S,Vento R,Chiaradonna F

    更新日期:2014-02-01 00:00:00

  • Does the lack of regression-associated mRNA expression render a rat ventral prostate epithelial cell line androgen independent?

    abstract::A series of rapidly dividing epithelial (RDE) cell lines have been isolated from primary cultures of rat ventral prostate (RVP) epithelial cells. Unlike androgen-dependent secretory epithelial cells, the RDE cells in culture do not express the androgen-dependent secretory proteins, nor do they express the androgen-rep...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240390308

    authors: Montpetit ML,Tenniswood MP

    更新日期:1989-03-01 00:00:00

  • Melanoma cancer vaccines and anti-tumor T cell responses.

    abstract::Melanoma is a disease which has been shown to be responsive to immune intervention. This has been suggested by reports of spontaneous responses of metastatic disease with strong immune infiltrates, and supported by recent data correlating clinical response after IFNalpha treatment with development of generalized autoi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.21473

    authors: Vujanovic L,Butterfield LH

    更新日期:2007-10-01 00:00:00

  • FAT-free p300 is good for scar-free tissue repair.

    abstract::Fibrosis, the deadly pathological manifestation of an abnormal tissue remodeling in any organ due to excessive collagen deposition, is associated with a wide variety of organ failure-related human diseases. Chronic stress or repeated injury in a particular organ induces abnormal molecular signals that lead to super-ac...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24820

    authors: Ghosh AK

    更新日期:2014-09-01 00:00:00

  • Decreased activity of acetyl-CoA carboxylase during chemically induced neutrophilic differentiation of human promyelocytic leukemia cells.

    abstract::In order to better understand the mechanism by which changes in the fatty acid composition of cellular lipids occur in leukemia cell lines induced to differentiate, the activity of the first enzyme of fatty acid biosynthesis, acetyl-CoA carboxylase (EC 6.4.1.2) was measured in HL-60 promyelocytic leukemia cells before...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240260203

    authors: Fischkoff SA,Papuchis GC,Nickols WA

    更新日期:1984-01-01 00:00:00

  • Retinoid signalling and gene expression in neuroblastoma cells: RXR agonist and antagonist effects on CRABP-II and RARbeta expression.

    abstract::9-cis Retinoic acid (RA) induces gene expression in neuroblastoma cells more effectively and with different kinetics than other RA isomers, and could be acting in part through Retinoid X Receptors (RXRs). The aim of this study was to characterise the effects of an RXR agonist and RXR homodimer antagonist on the induct...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10310

    authors: Hewson QC,Lovat PE,Pearson AD,Redfern CP

    更新日期:2002-01-01 00:00:00

  • LncRNA Erbb4-IR promotes esophageal squamous cell carcinoma (ESCC) by downregulating miR-145.

    abstract::Erbb4-IR is a recently identified lncRNA with pivotal functions in renal injury. The present study investigated the roles of Erbb4-IR in esophageal squamous cell carcinoma (ESCC). It was observed that Erbb4-IR was upregulated in tumor tissues of patients with ESCC. Plasma levels of Erbb4-IR in patients with ESCC were ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29023

    authors: Zhang Y,Zhang L,Wang R,Wang B,Hua P,Li J

    更新日期:2019-10-01 00:00:00

  • Inhibition of extracellular ATP-mediated lysis of human macrophages by calmodulin antagonists.

    abstract::Lysis of human culture-derived macrophages by extracellular ATP has recently been described, and treatment of macrophages with interferon-gamma rendered those cells significantly more sensitive to lysis. In addition, cell death occurred more rapidly in interferon (IFN)-treated cells than in untreated macrophages. In a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240570311

    authors: Blanchard DK,Hoffman SL,Djeu JY

    更新日期:1995-03-01 00:00:00

  • miR-381-3p restrains cervical cancer progression by downregulating FGF7.

    abstract::This study aimed at elucidating the molecular mechanism of miR-381-3p in cervical cancer progression, which may provide a novel therapeutic target for patients with cervical cancer. The expression of miR-381-3p was confirmed by quantitative reverse transcription polymerase chain reaction. Microarray analysis was condu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27438

    authors: Shang A,Zhou C,Bian G,Chen W,Lu W,Wang W,Li D

    更新日期:2019-01-01 00:00:00

  • Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation.

    abstract::Nuclear receptors (NRs) are key regulators of cell function and differentiation. We examined NR expression during osteogenic versus adipogenic differentiation of primary mouse calvarial osteoblasts (MOBs). MOBs were cultured for 21 days in osteogenic or adipogenic differentiation media. von Kossa and Oil Red O stainin...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21931

    authors: Pirih FQ,Abayahoudian R,Elashoff D,Parhami F,Nervina JM,Tetradis S

    更新日期:2008-12-01 00:00:00

  • Stable subclones of the chondrogenic murine cell line MC615 mimic distinct stages of chondrocyte differentiation.

    abstract::Fourteen stable subclones derived from the murine chondrogenic cell line MC615 were established and characterised regarding their differentiation stages and responsivity to BMP2. Based on their gene expression profiles which revealed remarkable variances in Col2a1 and Col10a1 expression, subclones could be grouped int...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22290

    authors: Surmann-Schmitt C,Widmann N,Mallein-Gerin F,von der Mark K,Stock M

    更新日期:2009-10-15 00:00:00

  • BMRP is a Bcl-2 binding protein that induces apoptosis.

    abstract::Members of the Bcl-2 family of proteins play important roles in the regulation of cell death by apoptosis. The yeast Two-Hybrid system was utilized to identify a protein that interacts with the anti-apoptotic protein Bcl-2, designated BMRP. This protein corresponds to a previously known mitochondrial ribosomal protein...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20292

    authors: Chintharlapalli SR,Jasti M,Malladi S,Parsa KV,Ballestero RP,González-García M

    更新日期:2005-02-15 00:00:00

  • Possible protective mechanisms exerted by metformin or metformin and vitamin E in isoproterenol-induced cardiac injury.

    abstract::Several studies have reported that metformin is cardioprotective for diabetic and non-diabetic ischemic hearts through mechanisms that cannot be entirely attributed to its anti-hyperglycemic effect. This study was designed to investigate the cardioprotective effects of metformin with and without vitamin E after induct...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26530

    authors: Al-Rasheed NM,Al-Rasheed NM,Al-Rabeeah DA,Al-Barrak HS,Al-Salman SA,Ibrahim SA,Al-Hassab SA,Al-Amin MA,Hasan IH,Al-Ajmi HN,Al-Shammari TK

    更新日期:2018-05-01 00:00:00

  • Altered hematopoietic stem cell and osteoclast precursor frequency in cathepsin K null mice.

    abstract::Cathepsin K (CatK) is a lysosomal cysteine protease necessary for bone resorption by osteoclasts (OCs), which originate from myeloid hematopoietic precursors. CatK-deficient (CatK(-/-) ) mice show osteopetrosis due to defective resorption by OCs, which are increased in number in these mice. We investigated whether gen...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24801

    authors: Jacome-Galarza C,Soung do Y,Adapala NS,Pickarski M,Sanjay A,Duong LT,Lorenzo JA,Drissi H

    更新日期:2014-08-01 00:00:00

  • Decreased DMT1 and increased ferroportin 1 expression is the mechanisms of reduced iron retention in macrophages by erythropoietin in rats.

    abstract::Recycled iron from reticuloendothelial macrophages to erythroid precursors is important to maintain the iron homeostasis. However, the molecular mechanisms underlying iron homeostasis in macrophages are poorly understood. In this study, male Sprague-Dawley rats were treated with recombinant human erythropoietin (rHuEp...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21654

    authors: Kong WN,Zhao SE,Duan XL,Yang Z,Qian ZM,Chang YZ

    更新日期:2008-05-15 00:00:00

  • Salinomycin suppresses LRP6 expression and inhibits both Wnt/β-catenin and mTORC1 signaling in breast and prostate cancer cells.

    abstract::Emerging evidence indicates that activation of Wnt/β-catenin signaling at the cell surface results in inhibition of glycogen synthase kinase 3β (GSK3β), leading to activation of mTORC1 signaling in cancer cells. The low density lipoprotein receptor-related protein-6 (LRP6) is an essential Wnt co-receptor for Wnt/β-cat...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24850

    authors: Lu W,Li Y

    更新日期:2014-10-01 00:00:00

  • PTH regulates β2-adrenergic receptor expression in osteoblast-like MC3T3-E1 cells.

    abstract::As the aged population is soaring, prevalence of osteoporosis is increasing. However, the molecular basis underlying the regulation of bone mass is still incompletely understood. Sympathetic tone acts via beta2 adrenergic receptors in bone and regulates the mass of bone which is the target organ of parathyroid hormone...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24953

    authors: Moriya S,Hayata T,Notomi T,Aryal S,Nakamaoto T,Izu Y,Kawasaki M,Yamada T,Shirakawa J,Kaneko K,Ezura Y,Noda M

    更新日期:2015-01-01 00:00:00

  • Effects of histone deacetylase inhibitors on p55CDC/Cdc20 expression in HT29 cell line.

    abstract::In a previous work, taking advantage of the gene-array screening technology, we analysed the effects of histone deacetylase (HDAC) inhibitor sodium butyrate (NaBt), on gene transcription in HT29 human adenocarcinoma cell line. In this study, we focused our attention on p55CDC/Cdc20 gene, whose expression was dramatica...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21014

    authors: Iacomino G,Medici MC,Napoli D,Russo GL

    更新日期:2006-11-01 00:00:00

  • Recent advances in understanding the mechanisms of osteoclast precursor fusion.

    abstract::Bone marrow macrophages fuse on the bone surface to form multinucleated osteoclasts that then organize to efficiently resorb bone. Many, if not all, of the stages of macrophage fusion involve cytoskeletal components that reorganize the cells. Recruitment may involve chemotactic responses to bone matrix protein and cal...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22640

    authors: Oursler MJ

    更新日期:2010-08-01 00:00:00

  • Protected regions in the chicken alpha 2(1) procollagen promoter in differentiated tissues.

    abstract::The higher ordered structure of the chicken alpha 2(1) procollagen gene was analyzed in chromatin isolated from expressing (lung) and nonexpressing (reticulocyte and erythrocyte) tissues. Digestion of DNA with methylation sensitive restriction endonucleases revealed that this gene was methylated in all tissues examine...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240540204

    authors: Truter SL,Parker MI

    更新日期:1994-02-01 00:00:00

  • Acidic fibroblast growth factor gene 5' non-coding exon and flanking region from hamster DDT1 cells: identification of the promoter region and transcriptional regulation by testosterone and aFGF protein.

    abstract::Selected clones of Syrian hamster DDT1-MF2 cells are responsive to testosterone for growth. Heparin binding growth factor 1 (HBGF-1) or acidic fibroblast growth factor (aFGF) can replace testosterone (T) in the stimulation of growth in these cells. This phenomena is correlated with testosterone's ability to elevate aF...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240510118

    authors: Hall JA,Harris MA,Intres R,Harris SE

    更新日期:1993-01-01 00:00:00

  • Augmented synthesis and differential localization of heparan sulfate proteoglycans in Duchenne muscular dystrophy.

    abstract::Muscular dystrophies are characterized by continuous cycles of degeneration and regeneration that result in extensive fibrosis and a progressive diminution of muscle mass. Cell surface heparan sulfate proteoglycans are found almost ubiquitously on the surface and in the extracellular matrix (ECM) of mammalian cells. T...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10184

    authors: Alvarez K,Fadic R,Brandan E

    更新日期:2002-01-01 00:00:00

  • Proteinase-Activated Receptor-2 Modulates Ve-Cadherin Expression to Affect Human Vascular Endothelial Barrier Function.

    abstract::Published data indicate that the protease-activated receptor (PAR) 2 is involved in the pathogenesis of some cardiovascular diseases; the underlying mechanism is to be further investigated. Ve-cadherin is a critical molecule in maintaining the endothelial barrier integrity. This study aims to investigate the role of P...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26123

    authors: Zhang R,Ge J

    更新日期:2017-12-01 00:00:00

  • Heparan sulfate proteoglycans as trastuzumab targets in anoikis-resistant endothelial cells.

    abstract::Anoikis is a form of programmed cell death induced by loss of contact from neighboring cells or from their extracellular matrix (ECM). Many tumorigenic cells are anoikis resistant, facilitating cancer progression and metastasis. Trastuzumab is a monoclonal antibody used for the treatment of breast and gastric cell can...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28656

    authors: Onyeisi JOS,Castanho de Almeida Pernambuco Filho P,de Araujo Lopes S,Nader HB,Lopes CC

    更新日期:2019-08-01 00:00:00

  • Organochlorine and organophosphorus pesticides and bladder cancer: A case-control study.

    abstract:BACKGROUND:Exposure to pesticides is associated with an increase in the incidence of cancer. We aimed to investigate the association of serum organochlorine pesticides (OCPs) and organophosphorus pesticides (OPs) levels and GSTM1/GSTT1 gene polymorphism with bladder cancer (BC). METHODS:This study was performed on 57 ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28746

    authors: Mortazavi N,Asadikaram G,Ebadzadeh MR,Kamalati A,Pakmanesh H,Dadgar R,Moazed V,Paydar P,Fallah H,Abolhassani M

    更新日期:2019-09-01 00:00:00

  • Wnt/β-catenin signaling enhances transcription of the CX43 gene in murine Sertoli cells.

    abstract::Sertoli cells provide the nutritional and metabolic support for germ cells. Wnt/β-catenin signaling is important for the development of the seminiferous epithelium during embryonic age, although after birth this pathway is downregulated. Cx43 gene codes for a protein that is critical during testicular development. The...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27973

    authors: López C,Aguilar R,Nardocci G,Cereceda K,Vander Stelt K,Slebe JC,Montecino M,Concha II

    更新日期:2019-04-01 00:00:00

  • Cytoplasmic proteins of porcine adipocytes: identification with monoclonal antibodies.

    abstract::In the present study, monoclonal antibodies were produced using porcine adipocyte extracts as the immunogen. Two of the monoclonal antibodies, designated CB6 and IB4, exhibited reactivity toward only cells containing lipid in stromal-vascular cell cultures. The antigens recognized by the CB6 and IB4 monoclonal antibod...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240450311

    authors: Wright JT,Hausman GJ

    更新日期:1991-03-01 00:00:00