Abstract:
:Burkitt lymphoma (BL) commonly exhibits Epstein-Barr virus (EBV) positivity associated with latent chronic infection. Models of acute EBV infection have been associated with cellular resistance to apoptosis. However, the effect of latent long-term EBV infection on apoptosis induced by drugs is not well defined. To determine this, we have studied the response of the Akata EBV+ cell line (type I latency) to etoposide, before and after downregulating EBV gene expression. We observed that downregulating EBV nuclear antigen-1 (EBNA-1) expression with siRNAs reverted cellular sensitivity to etoposide. In accordance with this finding, Akata EBV+ cells showed increased sensitivity to etoposide, when compared to the Akata EBV- cells. We also observed that Akata EBV+ cells presented increased apoptosis levels and decreased Bcl-xL mRNA and protein levels, when compared to the Akata EBV- cells. In addition, Akata EBV+ cells contained less endoplasmic reticulum (ER) than EBV- cells. Finally, downregulation of EBV with EBNA-1 siRNAs caused an increase in the expression of Bcl-xL indicating that EBV is responsible for the differences found between the Akata EBV+ and EBV- cell lines.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Lima RT,Seca H,Soares P,Nascimento MS,Vasconcelos MHdoi
10.1002/jcb.22920subject
Has Abstractpub_date
2011-01-01 00:00:00pages
200-10issue
1eissn
0730-2312issn
1097-4644journal_volume
112pub_type
杂志文章abstract::The mouse macrophage Fc gamma 2b/gamma 1R has previously been purified with the aid of the monoclonal antibody 2.4G2. That this Fc gamma R functions as a ligand-dependent ion channel is supported by the following evidence. Employing [3H]tetraphenylphosphonium ([3H]Ph4P+) as a probe for membrane potential changes in in...
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更新日期:1998-10-01 00:00:00
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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更新日期:1998-01-01 00:00:00
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更新日期:2007-02-15 00:00:00