Modulation of uptake of organic cationic drugs in cultured human colon adenocarcinoma Caco-2 cells by an ecto-alkaline phosphatase activity.

Abstract:

:Alkaline phosphatase (ALP) refers to a group of nonspecific phosphomonoesterases located primarily in cell plasma membrane. It has been described in different cell lines that ecto-ALP is directly or indirectly involved in the modulation of organic cation transport. We aimed to investigate, in Caco-2 cells, a putative modulation of 1-methyl-4-phenylpyridinium (MPP(+)) apical uptake by an ecto-ALP activity. Ecto-ALP activity and (3)H-MPP(+) uptake were evaluated in intact Caco-2 cells (human colon adenocarcinoma cell line), in the absence and presence of a series of drugs. The activity of membrane-bound ecto-ALP expressed on the apical surface of Caco-2 cells was studied at physiological pH using p-nitrophenylphosphate as substrate. The results showed that Caco-2 cells express ALP activity, characterized by an ecto-oriented active site functional at physiological pH. Genistein (250 micro M), 3-isobutyl-1-methylxanthine (1 mM), verapamil (100 micro M), and ascorbic acid (1 mM) significantly increased ecto-ALP activity and decreased (3)H-MPP(+) apical transport in this cell line. Orthovanadate (100 micro M) showed no effect on (3)H-MPP(+) transport and on ecto-ALP activity. On the other hand, okadaic acid (310 nM) and all trans-retinoic acid (1 micro M) significantly increased (3)H-MPP(+) uptake and inhibited ecto-ALP activity. There is a negative correlation between the effect of drugs upon ecto-ALP activity and (3)H-MPP(+) apical transport (r = -0.9; P = 0.0014). We suggest that apical uptake of organic cations in Caco-2 cells is affected by phosphorylation/dephosphorylation mechanisms, and that ecto-ALP activity may be involved in this process.

journal_name

J Cell Biochem

authors

Calhau C,Martel F,Hipólito-Reis C,Azevedo I

doi

10.1002/jcb.10306

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

408-16

issue

4

eissn

0730-2312

issn

1097-4644

journal_volume

87

pub_type

杂志文章
  • A proteomic approach to the identification of early molecular targets changed by L-ascorbic acid in NB4 human leukemia cells.

    abstract::The pro-oxidant effect of L-ascorbic acid (LAA) is toxic to leukemia cells. LAA induces the oxidation of glutathione to its oxidized form (GSSG) and this is followed by a concentration-dependent H(2)O(2) accumulation, which occurs in parallel to the induction of apoptosis. To identify early protein targets of LAA in l...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20971

    authors: Park S,Lee J,Yeom CH

    更新日期:2006-12-15 00:00:00

  • Caspase inhibition and N6-benzyladenosine-induced apoptosis in HL-60 cells.

    abstract::As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N(6)-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. Howeve...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1262

    authors: Mlejnek P

    更新日期:2001-01-01 00:00:00

  • Two modes of ERK activation by TNF in keratinocytes: different cellular outcomes and bi-directional modulation by vitamin D.

    abstract::Inflammation, elicited in the skin following tissue damage or pathogen invasion, may become chronic with deleterious consequences. Tumor necrosis factor (TNF) is a key mediator of cutaneous inflammation and the keratinocyte an important protagonist of skin immunity. Calcitriol, the hormonally active vitamin D metaboli...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21650

    authors: Ziv E,Rotem C,Miodovnik M,Ravid A,Koren R

    更新日期:2008-05-15 00:00:00

  • Biochemical evidence on the potential role of methyl mercury in hepatic glucose metabolism through inflammatory signaling and free radical pathways.

    abstract::Methylmercury (MeHg) is an extremely important environmental toxicant posing serious health risks to human health and a big source of environmental pollutant. Numerous evidence available showing a link between nervous system toxicity and MeHg exposure. Other forms of mercury are reason of metabolic toxic effects and a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28899

    authors: Maqbool F,Bahadar H,Hassani S,Niaz K,Baeeri M,Rahimifard M,Ghasemi-Niri SF,Abdollahi M

    更新日期:2019-09-01 00:00:00

  • Nucleolar spindle-associated protein 1 promotes tumorigenesis and predicts poor prognosis in human esophageal squamous cell carcinoma.

    abstract::The microtubule binding protein, nucleolar spindle-associated protein 1 (NUSAP1), has a crucial function in mitosis and its expression is closely associated with carcinogenesis. Herein, we aimed to determine the function of NUSAP1 in the development of human esophageal squamous cell carcinoma (ESCC), and the associati...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28452

    authors: Guan C,Liu Z,Lu C,Xiao M,Shi H,Ni R,Bian Z

    更新日期:2019-02-21 00:00:00

  • Reveromycin A-Induced Apoptosis in Osteoclasts Is Not Accompanied by Necrosis.

    abstract::Reveromycin A (RM-A), a small natural product isolated from Streptomyces bacteria, is a potential osteoporosis therapeutic in that it specifically induces apoptosis in osteoclasts but not osteoblasts. The purpose of the study presented here was to further elucidate the intracellular mechanisms of RM-A death effects in...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25125

    authors: Mead B,Morgan H,Mann-Knowlton A,Tedeschi L,Sloan C,Lang S,Hines C,Gragg M,Stofer J,Riemann K,Derr T,Heller E,Collins D,Landis P,Linna N,Jones D

    更新日期:2015-08-01 00:00:00

  • Differential effects of chemotherapeutic drugs versus the MDM-2 antagonist nutlin-3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B-cells.

    abstract::We have compared the cytotoxic/cytostatic responses of the SKW6.4 lymphoblastoid B-cells to the alkylating agent chlorambucil, the purine analog fludarabine, the non-genotoxic activator of the p53 pathway, Nutlin-3, used alone or in association with the death-inducing ligand recombinant TRAIL. Exposure to chlorambucil...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21649

    authors: Barbarotto E,Corallini F,Rimondi E,Fadda R,Mischiati C,Grill V,Vaccarezza M,Celeghini C

    更新日期:2008-05-15 00:00:00

  • Identification of NPAC as a novel biomarker and regulator for hepatocellular carcinoma.

    abstract:OBJECTIVE:Hepatocellular carcinoma (HCC) has a high morbidity and mortality around the world, yet the effective therapeutic option for HCC is still limited. NPAC, also known as glyoxylate reductase 1 homolog, is a new nuclear protein recently implicated in tumor biology. However, the role of NPAC in HCC remains unclear...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28106

    authors: Zhang J,Sun N,Guo W,Wu X,Yang X,Jin H,Zhang Y,Wu X,Zhang F,Hu L,Hu H,Gao Y

    更新日期:2018-11-26 00:00:00

  • Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR-126 in Primary Human Osteoblasts.

    abstract::Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs)...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25838

    authors: Strassburg S,Nabar N,Lampert F,Goerke SM,Pfeifer D,Finkenzeller G,Stark GB,Simunovic F

    更新日期:2017-07-01 00:00:00

  • Fastigial nucleus stimulation ameliorates cognitive impairment via modulating autophagy and inflammasomes activation in a rat model of vascular dementia.

    abstract:BACKGROUND:It has been reported that the fastigial nucleus (FSN) plays an important role in the development of vascular dementia (VD). Both autophagy and inflammation are functionally involved in the pathogenesis of VD. In this study, we aimed to evaluate the therapeutic effect of electrical cerebellar fastigial nucleu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27787

    authors: Xia D,Sui R,Min L,Zhang L,Zhang Z

    更新日期:2019-04-01 00:00:00

  • 3,4,5-trihydroxybenzaldehyde from Geum japonicum has dual inhibitory effect on matrix metalloproteinase 9; inhibition of gelatinoytic activity as well as MMP-9 expression in TNF-alpha induced HASMC.

    abstract::The matrix metalloproteinases (MMP-9 and MMP-2) production and smooth muscle cell (SMC) migration may play key roles in the pathogenesis of atherosclerotic lesions. In particular, the cancer cell invasion and SMC migration through vascular wall were shown to be directly associated with inducible MMP-9 expression. Prev...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21854

    authors: Suh SJ,Cho KJ,Moon TC,Chang HW,Park YG,Kim CH

    更新日期:2008-10-01 00:00:00

  • Differential regulation of cyclooxygenase-2 and inducible nitric oxide synthase by 4-hydroxynonenal in human osteoarthritic chondrocytes through ATF-2/CREB-1 transactivation and concomitant inhibition of NF-kappaB signaling cascade.

    abstract::4-hydroxynonenal (HNE), a lipid peroxidation end product, is produced abundantly in osteoarthritic (OA) articular tissues and was recently identified as a potent catabolic factor in OA cartilage. In this study, we provide additional evidence that HNE acts as an inflammatory mediator by elucidating the signaling cascad...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21110

    authors: Vaillancourt F,Morquette B,Shi Q,Fahmi H,Lavigne P,Di Battista JA,Fernandes JC,Benderdour M

    更新日期:2007-04-01 00:00:00

  • Regulation of amino acid-sensitive TOR signaling by leucine analogues in adipocytes.

    abstract::In adipocytes, amino acids stimulate the target of rapamycin (TOR) signaling pathway leading to phosphorylation of the translational repressor, eIF-4E binding protein-I (4E-BP1), and ribosomal protein S6. L-leucine is the primary mediator of these effects. The structure-activity relationships of a putative L-leucine r...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(20000501)77:2<234::aid-jcb

    authors: Lynch CJ,Fox HL,Vary TC,Jefferson LS,Kimball SR

    更新日期:2000-03-01 00:00:00

  • Albumin induces cellular fibrosis by upregulating transforming growth factor-beta ligand and its receptors in renal distal tubule cells.

    abstract::Albuminuria is indicative of nephropathy. However, little literature has focused on the role of albumin in renal distal tubule fibrosis. We used a well-defined distal tubule cell, Madin-Darby Canine Kidney (MDCK). Proliferation and cytotoxicity were examined. The conditioned supernatant was collected and subjected to ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20673

    authors: Liu HC,Liao TN,Lee TC,Chuang LY,Guh JY,Liu SF,Hu MS,Yang YL,Lin SH,Hung MY,Huang JS,Hung TJ,Chen CD,Chiang TA,Chan JY,Chen SY,Yang YL

    更新日期:2006-04-01 00:00:00

  • Evidence for inhibition of MyEF-2 binding to MBP promoter by MEF-1/Pur alpha.

    abstract::Myelin basic protein (MBP) is a major component of the myelin sheath whose production is developmentally controlled during myelinogenesis. Earlier studies have indicated that programmed expression of the MBP gene is regulated at the level of transcription. Evidently, the MB1 regulatory motif located between nucleotide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19970915)66:4<524::aid-jcb

    authors: Muralidharan V,Tretiakova A,Steplewski A,Haas S,Amini S,Johnson E,Khalili K

    更新日期:1997-09-15 00:00:00

  • Effects of c-Jun and a negative dominant mutation of c-Jun on differentiation and gene expression in lens epithelial cells.

    abstract::We have used a retroviral vector (RCAS) to overexpress wild-type chicken c-Jun or a deletion mutant of chicken c-Jun (Jun delta 7) lacking the DNA binding region to investigate the possible role of c-Jun in lens epithelial cell proliferation and differentiation. Both constructs were efficiently expressed in primary cu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240580212

    authors: Rinaudo JA,Vacchiano E,Zelenka PS

    更新日期:1995-06-01 00:00:00

  • LncRNA MALAT-1 Elevates HMGB1 to Promote Autophagy Resulting in Inhibition of Tumor Cell Apoptosis in Multiple Myeloma.

    abstract::Long non-coding RNAs (lncRNAs) can participate in the pathological process of multiple myeloma (MM) via regulation of specific gene expression and function. This research aimed to study the role of MALAT-1 and the underlying mechanism in MM. In this study, the expression of MALAT-1 and HMGB1 protein in the bone marrow...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25987

    authors: Gao D,Lv AE,Li HP,Han DH,Zhang YP

    更新日期:2017-10-01 00:00:00

  • Arsenite stimulates cyclooxygenase-2 expression through activating IkappaB kinase and nuclear factor kappaB in primary and ECV304 endothelial cells.

    abstract::Epidemiological studies have shown that chronic exposure to arsenic can result in liver injury, peripheral neuropathy, arteriosclerosis, and an increased incidence of cancer of the lung, skin, bladder, and liver. The overexpression of inducible cyclooxygenase-2 (Cox-2) has been associated with vascular inflammation an...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10096

    authors: Tsai SH,Liang YC,Chen L,Ho FM,Hsieh MS,Lin JK

    更新日期:2002-01-01 00:00:00

  • Upregulation of long noncoding RNA TUG1 contributes to the development of laryngocarcinoma by targeting miR-145-5p/ROCK1 axis.

    abstract::Laryngocarcinoma is the most common head and neck cancer and has a high incidence and mortality, causing about 83 000 deaths per year worldwide. Our research aimed to investigate the possible role of long noncoding RNA (lncRNA) taurine upregulated gene 1 (TUG1) in laryngocarcinoma development. The messenger RNA (mRNA)...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28614

    authors: Zhuang S,Liu F,Wu P

    更新日期:2019-08-01 00:00:00

  • Extracellular-regulated kinase-mitogen-activated protein kinase cascade: unsolved issues.

    abstract::This review point out several aspects regarding the mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase (Erk) network, which are still pending issues in the understanding how this pathway integrate information to drive cell fates. Focusing on the role of Erk during cell cycle, it has to be underline...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.22477

    authors: Bodart JF

    更新日期:2010-04-01 00:00:00

  • Protein-tyrosine phosphatases and the regulation of insulin action.

    abstract::Protein-tyrosine phosphatases (PTPases) play an important role in the regulation of insulin action by dephosphorylating the active (autophosphorylated) form of the insulin receptor and attenuating its tyrosine kinase activity. PTPases can also modulate post-receptor signalling by catalyzing the dephosphorylation of ce...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240480107

    authors: Goldstein BJ

    更新日期:1992-01-01 00:00:00

  • Effects of S100A12 gene silencing on serum levels of anti-inflammatory/pro-inflammatory cytokines in septic rats through the ERK signaling pathway.

    abstract::We explored the effect of S100A12 gene on serum levels of anti-inflammatory/pro-inflammatory cytokines in septic rats by activating the extracellular signal-regulated kinase (ERK) signaling pathway. A total of 180 specific pathogen-free (SPF) rats were purchased to establish cecal ligation and puncture (CLP) model. Ra...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26568

    authors: Wen X,Han XR,Wang YJ,Fan SH,Zhang ZF,Wu DM,Lu J,Zheng YL

    更新日期:2018-05-01 00:00:00

  • Tamoxifen induces TGF-beta 1 activity and apoptosis of human MCF-7 breast cancer cells in vitro.

    abstract::We report here that the antiestrogen tamoxifen (TAM) induces cell death in human breast cancer cell line MCF-7. We assessed the type of cell death induced by TAM in this breast cancer cell line on the basis of morphological and biochemical characteristics. Dying cells showed morphological characteristics of apoptosis,...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4644(19960401)61:1%3C9::AID-JCB

    authors: Chen H,Tritton TR,Kenny N,Absher M,Chiu JF

    更新日期:1996-04-01 00:00:00

  • Three-dimensional co-culture facilitates the differentiation of embryonic stem cells into mature cardiomyocytes.

    abstract::The cardiomyocyte (CM) differentiation of embryonic stem cells (ESCs) is routinely cultured as two-dimensional (2D) monolayer, which doesn't mimic in vivo physiological environment and may lead to low differentiated level of ESCs. Here, we develop a novel strategy that enhances CM differentiation of ESCs in collagen m...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23283

    authors: Ou DB,He Y,Chen R,Teng JW,Wang HT,Zeng D,Liu XT,Ding L,Huang JY,Zheng QS

    更新日期:2011-12-01 00:00:00

  • Development, characterization, and applications of a novel estrogen receptor beta monoclonal antibody.

    abstract::The role of estrogen receptor alpha (ERα) in breast cancer has been studied extensively, and its protein expression is prognostic and a primary determinant of endocrine sensitivity. However, much less is known about the role of ERβ and its relevance remains unclear due to the publication of conflicting reports. Here, ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23443

    authors: Wu X,Subramaniam M,Negron V,Cicek M,Reynolds C,Lingle WL,Goetz MP,Ingle JN,Spelsberg TC,Hawse JR

    更新日期:2012-02-01 00:00:00

  • Increasing and decreasing protein stability: effects of revertant substitutions on the thermal denaturation of phage lambda repressor.

    abstract::The thermal denaturations of five revertant lambda repressors containing single amino acid substitutions in their N-terminal domains have been studied by differential scanning calorimetry. Two substitutions slightly decrease stability, and the remaining three render the protein more stable than wild type. The Gly48---...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240290306

    authors: Hecht MH,Hehir KM,Nelson HC,Sturtevant JM,Sauer RT

    更新日期:1985-01-01 00:00:00

  • Proteomic analysis of the function of spot in Helicobacter pylori anti-oxidative stress in vitro and colonization in vivo.

    abstract::As a microaerobe, Helicobacter pylori employs the global regulator SpoT for defending against oxidative stress in vitro. However, the mechanisms how SpoT affects bacterial gene expression is still unknown. Moreover, the function of SpoT in H. pylori colonization in the host is remaining undetermined. To explore the fu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24215

    authors: Sun Y,Li X,Li W,Zhao M,Wang L,Liu S,Zeng J,Liu Z,Jia J

    更新日期:2012-11-01 00:00:00

  • Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL-6 and IL-8 in pulmonary fibrosis in rabbits.

    abstract::Panax notoginseng saponin (PNS) constitutes the major effective components of Panax notoginseng, which is widely used to treat microcirculatory disturbance associated diseases. In this study, we designed to investigate the effect of PNS on the treatment of pulmonary fibrosis (PF) and further explored its mechanism. A ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27045

    authors: Zhang J,Li Q,Shao Q,Song J,Zhou B,Shu P

    更新日期:2018-11-01 00:00:00

  • Association of TNF-308 G>A polymorphism located in tumor necrosis factor a with the risk of developing cervical cancer and results of pap smear.

    abstract::Tumor necrosis factor a (TNFa) is an inflammatory cytokine that plays a crucial role in the immune response and the progression of cervical lesions. There is a growing body of data evaluating the value of a genetic variant in the TNFa gene with the risk of developing cervical cancer. The aim of this study was to explo...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27823

    authors: Babapour N,Mehramiz M,Rastgar Moghadam A,Behboodi N,Yousefi Z,Maftouh M,Talebian S,Khazaei M,Jafarian A,Sharifi-Sistani N,Avan A,Hasanzadeh M

    更新日期:2019-04-01 00:00:00

  • A3 Adenosine Receptor Agonist Inhibited Survival of Breast Cancer Stem Cells via GLI-1 and ERK1/2 Pathway.

    abstract::Numerous studies have demonstrated the role of A3 adenosine receptor (A3AR) and signaling pathways in the multiple aspects of the tumor. However, there is a little study about the function of A3AR in the biological processes of cancer stem cells (CSCs). CSCs have a critical role in the maintenance and survival of brea...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25945

    authors: Jafari SM,Panjehpour M,Aghaei M,Joshaghani HR,Enderami SE

    更新日期:2017-09-01 00:00:00