Effect of simvastatin in different concentrations on free radicals in A-2058 human melanoma malignum cells-EPR studies.

Abstract:

:The influence of concentration of simvastatin (SIM) on free radicals in A-2058 human melanoma malignum cells was studied. The proliferation assay for melanoma A-2058 cells with SIM in concentration range from 0.1 to 20 µM was performed. SIM in the concentrations of 0.1, 0.3, and 1 μM only slightly changed the growth of A-2058 cells, but the growth of the cells considerably decreased for higher concentrations of SIM. Free radicals in the cells were examined by an X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy. o-Semiquinone free radicals with g-factors in the range of 2.0060 to 2.0065 were found in A-2058 cells. The asymmetric broad EPR spectra with linewidths (ΔBpp) from 0.87 to 1.25 mT were measured. The fast spin-lattice relaxation processes characterized all the tested cells. The free radical concentrations in the all A-2058 cells cultured with SIM were lower than in the control cells. The quenching of free radicals in A-2058 cells depended on concentration of SIM. This effect was the weakest for concentration of SIM of 3 μM. The strongest decrease of free radical concentration caused SIM in concentration of 1 μM.

journal_name

J Cell Biochem

authors

Zdybel M,Chodurek E,Pilawa B

doi

10.1002/jcb.27540

subject

Has Abstract

pub_date

2018-09-11 00:00:00

eissn

0730-2312

issn

1097-4644

pub_type

杂志文章
  • Melatonin reduces two-cell block via nonreceptor pathway in mice.

    abstract::Embryo development block seriously limits the success of in vitro embryo production and assisted reproductive technology. Although numerous researchers have explored this problem, it remains to be solved. In this study, we found that melatonin supplementation at 10-8 and 10-9 M in M16 significantly reduced two-cell bl...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27255

    authors: Shi F,Li H,Wang E,Chen ZJ,Zhang C

    更新日期:2018-11-01 00:00:00

  • Analgesic-antitumor peptide inhibits proliferation and migration of SHG-44 human malignant glioma cells.

    abstract::Malignant gliomas, the most common subtype of primary brain tumors, are characterized by high proliferation, great invasion, and neurological destruction and considered to be the deadliest of human cancers. Analgesic-antitumor peptide (AGAP), one of scorpion toxic polypeptides, has been shown to have antitumor activit...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23166

    authors: Zhao Y,Cai X,Ye T,Huo J,Liu C,Zhang S,Cao P

    更新日期:2011-09-01 00:00:00

  • microRNA-145-3p inhibits non-small cell lung cancer cell migration and invasion by targeting PDK1 via the mTOR signaling pathway.

    abstract::The mammalian target of rapamycin (mTOR) pathway is dysregulated in more than 50% of all human malignancies and is a major target in cancer treatment. In this study, we explored the underlying mechanism involving microRNA-145-3p (miR-145-3p) in the development and progression of non-small cell lung cancer (NSCLC) by t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26252

    authors: Chen GM,Zheng AJ,Cai J,Han P,Ji HB,Wang LL

    更新日期:2018-01-01 00:00:00

  • In vivo antibacterial activity of Zataria multiflora Boiss extract and its components, carvacrol, and thymol, against colistin-resistant Acinetobacter baumannii in a pneumonic BALB/c mouse model.

    abstract:BACKGROUND:Acinetobacter baumannii has emerged as a major cause of nosocomial infections. Various resistance mechanisms of A. baumannii against antibiotics have transformed it into a successful nosocomial pathogen. Because of the limited number of available antibiotics, we used a medicinal plant with an antibacterial e...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28908

    authors: Hassannejad N,Bahador A,Rudbari NH,Modarressi MH,Parivar K

    更新日期:2019-11-01 00:00:00

  • Scleraxis messenger ribonucleic acid is expressed in C2C12 myoblasts and its level is down-regulated by bone morphogenetic protein-2 (BMP2).

    abstract::We examined the mRNA expression of scleraxis, a non-myogenic helix-loop-helix type transcription factor in C2C12 myogenic cells. Scleraxis mRNA has been shown to be expressed in sclerotome and perichondrium of the embryos. We found that C2C12 cells express 1.2 kb scleraxis mRNA constitutively. Since BMP was reported t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19971001)67:1<66::aid-jcb7

    authors: Liu Y,Nifuji A,Tamura M,Wozney JM,Olson EN,Noda M

    更新日期:1997-10-01 00:00:00

  • Nucleolar spindle-associated protein 1 promotes tumorigenesis and predicts poor prognosis in human esophageal squamous cell carcinoma.

    abstract::The microtubule binding protein, nucleolar spindle-associated protein 1 (NUSAP1), has a crucial function in mitosis and its expression is closely associated with carcinogenesis. Herein, we aimed to determine the function of NUSAP1 in the development of human esophageal squamous cell carcinoma (ESCC), and the associati...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28452

    authors: Guan C,Liu Z,Lu C,Xiao M,Shi H,Ni R,Bian Z

    更新日期:2019-02-21 00:00:00

  • High glucose condition suppresses neurosphere formation by human periodontal ligament-derived mesenchymal stem cells.

    abstract::D-Glucose serves many roles in cellular functions, but its role in human periodontal ligament-derived mesenchymal stem cells (hPSLSCs) is yet unknown. Here, the roles of high glucose concentration on neurogenic differentiation by hPDLSCs were investigated. Two-stage neurogenic induction protocol was employed. Cells we...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24735

    authors: Sawangmake C,Pavasant P,Chansiripornchai P,Osathanon T

    更新日期:2014-05-01 00:00:00

  • Inorganic phosphate as a signaling molecule in osteoblast differentiation.

    abstract::The spatial and temporal coordination of the many events required for osteogenic cells to create a mineralized matrix are only partially understood. The complexity of this process, and the nature of the final product, demand that these cells have mechanisms to carefully monitor events in the extracellular environment ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10622

    authors: Beck GR Jr

    更新日期:2003-10-01 00:00:00

  • Prediction of platinum-resistance patients of gastric cancer using bioinformatics.

    abstract::Lack of guidelines for personalized chemotherapy treatment after surgery has caused gastric cancer (GC) patients' unnecessary exposure to toxicity and the financial burden of chemotherapy treatments. In our study, we aimed to identify potential biomarkers to predict GC patients' susceptibility to platinum-based on Gen...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28621

    authors: Pan J,Xiang Z,Dai Q,Wang Z,Liu B,Li C

    更新日期:2019-08-01 00:00:00

  • Activation of the p38 MAPK/Akt/ERK1/2 signal pathways is required for the protein stabilization of CDC6 and cyclin D1 in low-dose arsenite-induced cell proliferation.

    abstract::Arsenic trioxide (ATO) is a first-line anti-cancer agent for acute promyelocytic leukemia, and induces apoptosis in other solid cancer cell lines including breast cancer cells. However, as with arsenites found in drinking water and used as raw materials for wood preservatives, insecticides, and herbicides, low doses o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22886

    authors: Liu Y,Hock JM,Sullivan C,Fang G,Cox AJ,Davis KT,Davis BH,Li X

    更新日期:2010-12-15 00:00:00

  • The beta3 chain short arm of laminin-332 (laminin-5) induces matrix assembly and cell adhesion activity of laminin-511 (laminin-10).

    abstract::The basement membrane (BM) protein laminin-332 (Lm332) (laminin-5) has unique activity and structure as compared with other laminins: it strongly promotes cellular adhesion and migration, and its alpha3, beta3, and gamma2 chains are all truncated in their N-terminal regions (short arms). In the present study, we inves...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21032

    authors: Nakashima Y,Kariya Y,Miyazaki K

    更新日期:2007-02-15 00:00:00

  • Modulation of human cytomegalovirus immediate-early gene enhancer by mitogen-activated protein kinase kinase kinase-1.

    abstract::The immediate-early (IE) promoter of human cytomegalovirus (HCMV) constitutes a primary genetic switch, which determines the progression of viral infection. Earlier reports by others have shown mitogen-activated protein kinase kinase kinase-1 (MEKK1) to be able to up-regulate HCMV-IE promoter through downstream mitoge...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1251

    authors: Sun B,Harrowe G,Reinhard C,Yoshihara C,Chu K,Zhuo S

    更新日期:2001-01-01 00:00:00

  • DACH1 negatively regulates the human RANK ligand gene expression in stromal/preosteoblast cells.

    abstract::Receptor activator of NF-kappaB ligand (RANKL) is a critical osteoclastogenic factor that is expressed on bone marrow stromal/preosteoblast cells. Most bone resorption stimuli induce osteoclast formation by modulating RANKL expression in these cells. However, little is known about the mechanisms regulating RANKL gene ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21561

    authors: Sundaram K,Mani SK,Kitatani K,Wu K,Pestell RG,Reddy SV

    更新日期:2008-04-15 00:00:00

  • Does the lack of regression-associated mRNA expression render a rat ventral prostate epithelial cell line androgen independent?

    abstract::A series of rapidly dividing epithelial (RDE) cell lines have been isolated from primary cultures of rat ventral prostate (RVP) epithelial cells. Unlike androgen-dependent secretory epithelial cells, the RDE cells in culture do not express the androgen-dependent secretory proteins, nor do they express the androgen-rep...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240390308

    authors: Montpetit ML,Tenniswood MP

    更新日期:1989-03-01 00:00:00

  • MicroRNA-424 regulates epithelial-mesenchymal transition of endometrial carcinoma by directly targeting insulin-like growth factor 1 receptor.

    abstract::Although numerous miRNAs are reported to contribute to the carcinogenesis of malignant tumor, the specific role of miR-424 in endometrial carcinoma is seldom reported. To explore the effect of miR-424 on epithelial-mesenchymal transition and its underlying mechanism, we detected miR-424 expression in endometrial carci...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27528

    authors: Shu S,Liu X,Xu M,Gao X,Fan J,Liu H,Li R

    更新日期:2018-09-06 00:00:00

  • Identification of novel prognostic alternative splicing signature in papillary renal cell carcinoma.

    abstract::Papillary renal cell carcinoma (pRCC) is a heterogeneous disease containing multifocal or solitary tumors with an aggressive phenotype. Increasing evidence has indicated the involvement of aberrant splicing variants in renal cell cancer, while systematic profiling of aberrant alternative splicing (AS) in pRCC was lack...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29314

    authors: Duan Y,Zhang D

    更新日期:2020-01-01 00:00:00

  • Overexpression of miR-4286 is an unfavorable prognostic marker in individuals with non-small cell lung cancer.

    abstract::Non-small cell lung cancer (NSCLC) is still an unresolved source of tumor-related death internationally. Current studies have discovered that microRNAs (miRNAs) are associated with diverse cancers development, including NSCLC. Our paper focused on the functional character of miR-4286 in NSCLC. miR-4286 level in 68 cas...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29024

    authors: An X,Ge J,Guo H,Mi H,Zhou J,Liu Y,Weiyue,Wu Z

    更新日期:2019-10-01 00:00:00

  • Genetic diversity of Paramecium species on the basis of multiple loci analysis and ITS secondary structure models.

    abstract::Among ciliates, Paramecium has become a privileged model for the study of "species problem" particularly in the case of the "Paramecium aurelia complex" that has been intensely investigated. Despite extensive studies, the taxonomy of Paramecium is still challenging. The major problem is an uneven sampling of Parameciu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29546

    authors: Tasneem F,Shakoori FR,Ilyas M,Shahzad N,Potekhin A,Shakoori AR

    更新日期:2019-11-06 00:00:00

  • Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer.

    abstract::Helicobacter pylori (H. pylori) infection causes chronic gastritis and peptic ulceration and is the strongest risk factor for the development of gastric cancer. The pathogenesis of H. pylori is believed to be associated with infection-initiated chronic gastritis, which is characterized by enhanced expression of many i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.24389

    authors: Lamb A,Chen LF

    更新日期:2013-03-01 00:00:00

  • In support of fatty acid synthase (FAS) as a metabolic oncogene: extracellular acidosis acts in an epigenetic fashion activating FAS gene expression in cancer cells.

    abstract::Relatively little information exists on the ultimate molecular mechanisms by which the lipogenic enzyme Fatty Acid Synthase (FAS) is differentially overexpressed in a biologically aggressive subset of human malignancies. Since the microenvironment of solid tumors contains regions of poor oxygenation and high acidity, ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20310

    authors: Menendez JA,Decker JP,Lupu R

    更新日期:2005-01-01 00:00:00

  • Role of vascular endothelial growth factor in the communication between human osteoprogenitors and endothelial cells.

    abstract::Proper bone remodeling requires an active process of angiogenesis which in turn supplies the necessary growth factors and stem cells. This tissue cooperation suggests a cross-talk between osteoblasts and endothelial cells. This work aims to identify the role of paracrine communication through vascular endothelial grow...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22018

    authors: Grellier M,Ferreira-Tojais N,Bourget C,Bareille R,Guillemot F,Amédée J

    更新日期:2009-02-15 00:00:00

  • Myoblast and myotube nuclei display similar patterns of heterogeneous acetylcholine receptor subunit mRNA expression.

    abstract::Muscle progenitor cells differentiate to myoblasts, and subsequently myotubes, upon expression of muscle specific genes. We and others have previously shown that myotube nuclei, even in the absence of nerve, express AChR alpha subunit RNA at varying levels, with a small subset (about ten percent) of the nuclei express...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240580105

    authors: Su X,Berman SA,Sullivan T,Bursztajn S

    更新日期:1995-05-01 00:00:00

  • Binding motifs of CBP2 a potential cell surface target for carcinoma cells.

    abstract::Previously we have shown (Hebert et al. [1999] J. Cell Biochem. 73:248-258) that among many cell lines the CBP2 gene product, Hsp47, eludes its retention receptor, erd2P, resulting in the appearance of Hsp47 on the cell surface associated with the tetraspanin protein CD9. Since Hsp47 possesses a highly restricted bind...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Sauk JJ,Coletta RD,Norris K,Hebert C

    更新日期:2000-05-01 00:00:00

  • SIRT1: a novel target to prevent atherosclerosis.

    abstract::Atherosclerosis is a chronic immuno-inflammatory disease associated with blood lipids disorder. Many studies have demonstrated that caloric restriction (CR) can prevent atherosclerosis and extend lifespan. Sir2 protein, mammal's SIRT1, has been reported to at least partly contribute to the protective effect of CR. Hen...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22240

    authors: Yu W,Fu YC,Chen CJ,Wang X,Wang W

    更新日期:2009-09-01 00:00:00

  • LncRNA CRNDE promotes hepatocellular carcinoma progression by upregulating SIX1 through modulating miR-337-3p.

    abstract:BACKGROUND:Long noncoding RNA (lncRNA) is emerging as a vital regulator in various cancers. Recently, it was found that lncRNA colorectal neoplasia differentially expressed (CRNDE) plays an oncogenic role, promoting cell proliferation and migration in hepatocellular carcinoma (HCC). However, the underlying regulatory m...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28894

    authors: Tang D,Zhao L,Peng C,Ran K,Mu R,Ao Y

    更新日期:2019-09-01 00:00:00

  • Interferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells.

    abstract::Adequate regulation of endolymphatic pH is essential for maintaining inner ear function. The Na(+)-H(+) exchanger (NHE) is a major determinant of intracellular pH (pH(i)), and facilitates Na(+) and fluid absorption in various epithelia. We determined the functional and molecular expression of NHEs in cultured human en...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22201

    authors: Son EJ,Moon IS,Kim SH,Kim SJ,Choi JY

    更新日期:2009-08-01 00:00:00

  • Intrinsic or acquired drug resistance and metastasis: are they linked phenotypes?

    abstract::Evidence is reviewed which suggests a linkage may exist between certain forms of de novo or acquired drug resistance and metastasis. This includes the finding that expression of certain dominantly acting mutant oncogenes or tumor suppressor genes, i.e., genes which normally act to "drive" tumor progression and metasta...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240560108

    authors: Kerbel RS,Kobayashi H,Graham CH

    更新日期:1994-09-01 00:00:00

  • IL-15 and RANKL Play a Synergistically Important Role in Osteoclastogenesis.

    abstract::Interleukin-15 (IL-15), a cytokine secreted by several cell types, has important physiological roles in the activity, proliferation, and viability of immune cells. It has both chemoattractant and proinflammatory properties, and may promote bone destruction. A previous study has shown that IL-15 alone exerts no effect ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25726

    authors: Okabe I,Kikuchi T,Mogi M,Takeda H,Aino M,Kamiya Y,Fujimura T,Goto H,Okada K,Hasegawa Y,Noguchi T,Mitani A

    更新日期:2017-04-01 00:00:00

  • Jedi--a novel transmembrane protein expressed in early hematopoietic cells.

    abstract::Self-renewal and differentiation of hematopoietic stem and progenitor cells are defined by the ensembles of genes expressed by these cells. Here we report identification of a novel gene named Jedi, which is expressed predominantly in short- and long-term repopulating stem cells when compared to more mature bone marrow...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21232

    authors: Krivtsov AV,Rozov FN,Zinovyeva MV,Hendrikx PJ,Jiang Y,Visser JW,Belyavsky AV

    更新日期:2007-06-01 00:00:00

  • Differentially expressed circular RNAs in maternal and neonatal umbilical cord plasma from SGA compared with AGA.

    abstract::Small for gestational age (SGA) has a high risk of mortality and morbidity and is common in obstetrics. To date, no effective prediction and treatment tools are available. Acting as microRNA (miRNA) sponges and disease biomarkers are clear functions of circular RNAs (circRNAs). However, it is still unknown what role c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29317

    authors: Wang Y,Li SF,Dang YJ,Shi XM,Chen L,Wang N,Cai Y,Zhao YY

    更新日期:2020-01-01 00:00:00