Abstract:
BACKGROUND:Integrin αIIbβ3 mediates platelet adhesion, aggregation and fibrin clot retraction. These processes require activation of αIIbβ3 and post-ligation signaling. Disulfide bond exchanges are involved in αIIbβ3 and αvβ3 activation. METHODS:In order to investigate the role of integrin activation and disulfide bond exchange during αIIbβ3- and αvβ3-mediated clot retraction, we co-expressed in baby hamster kidney cells wild-type (WT) human αIIb and WT or mutated human β3 that contain single or double cysteine substitutions disrupting C523-C544 or C560-C583 bonds. Flow cytometry was used to measure surface expression and activation state of the integrins. Time-course of fibrin clot retraction was examined. RESULTS:Cells expressed WT or mutated human αIIbβ3 as well as chimeric hamster/human αvβ3. The αIIbβ3 mutants were constitutively active and the thiol blocker dithiobisnitrobenzoic acid (DTNB) did not affect their activation state. WT cells retracted the clot and addition of αvβ3 inhibitors decreased the retraction rate. The active mutants and WT cells activated by anti-LIBS6 antibody retracted the clot faster than untreated WT cells, particularly in the presence of αvβ3 inhibitor. DTNB substantially inhibited clot retraction by WT or double C523S/C544S mutant expressing cells, but minimally affected single C523S, C544S or C560S mutants. Anti-LIBS6-enhanced clot retraction was significantly inhibited by DTNB when added prior to anti-LIBS6. CONCLUSIONS:Both αIIbβ3 and αvβ3 contribute to clot retraction without prior activation of the integrins. Activation of αIIbβ3, but not of αvβ3 enhances clot retraction. Both αIIbβ3 activation and post-ligation signaling during clot retraction require disulfide bond exchange.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Mor-Cohen R,Rosenberg N,Averbukh Y,Seligsohn U,Lahav Jdoi
10.1016/j.thromres.2014.02.001subject
Has Abstractpub_date
2014-05-01 00:00:00pages
826-36issue
5eissn
0049-3848issn
1879-2472pii
S0049-3848(14)00064-4journal_volume
133pub_type
杂志文章abstract::D-dimer is an indicator for in vivo fibrin formation, reflecting the formation of fibrin crosslinked by factor XIIIa. D-dimer assays are frequently used in emergency situations, such as diagnosis of venous thrombosis and pulmonary embolism, or disseminated intravascular coagulation. In these conditions, short sample t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.10.020
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Nuclear factor kappa B (NF-kappa B) activation and tissue factor (TF) expression may contribute to lethality in sepsis. Inappropriate in vivo expression of TF is likely responsible for fibrin deposition in sepsis-associated disseminated intravascular coagulation (DIC). Clinical assessment of TF expression ha...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2003-01-01 00:00:00
abstract::Ovarian hyperstimulation syndrome (OHSS) affects 5% of IVF cycles and incurs a 100-fold increase in risk of venous thrombosis over natural conceptions. Identification of women at risk of OHSS can be achieved using antral follicle count (AFC) and anti-Müllerian hormone (AMH). For those women with a high AFC or AMH comb...
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更新日期:2013-01-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章,评审
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更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:Although patients with idiopathic VTE are at higher than normal risk of asymptomatic atherosclerosis and of cardiovascular events, the impact of cardiovascular risk factors on VTE is poorly understood. OBJECTIVE:To assess the prevalence of the metabolic syndrome and of its components in patients with early-...
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更新日期:2012-09-01 00:00:00
abstract::Endothelial cells synthesize and release different proteins involved in their function, and some of these proteins may play important roles in the cellular response to injury, infection, or glucocorticoids. We have examined the profile of proteins released from rabbit coronary microvascular endothelial (RCME) and huma...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90202-n
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abstract::The effect of the combined oral administration of pentoxifylline (pof) and low dose acetylsalicylic acid (ASA) was evaluated with the help of the laser-induced thrombosis in rat mesenteric arterioles. Laser-induced thrombosis is inhibited in a dose-dependent way by both drugs. The administration of ASA, either simulta...
journal_title:Thrombosis research
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更新日期:1987-04-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.06.007
更新日期:2006-01-01 00:00:00
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更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Deep vein thrombosis (DVT) is a common multi-factorial disease with a partially understood aetiology. Although the roles of high factor (F)VIII and von Willebrand factor (VWF) levels are recognized, that of ADAMTS13 is still unclear. AIM:To assess the association between ADAMTS13 activity levels, VWF antige...
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pub_type: 杂志文章
doi:10.1016/0049-3848(83)90243-8
更新日期:1983-02-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.04.005
更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0049-3848(96)00098-9
更新日期:1996-06-15 00:00:00
abstract:INTRODUCTION:Patients with type 1 diabetes have high risk of developing microvascular complications, and microangiopathy contributes to premature cardiovascular disease in this population. The role that microvesicles (MVs) may play in the development of microangiopathy in type 1 diabetes remains unclear. MATERIALS AND...
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pub_type: 杂志文章
doi:10.1016/j.thromres.2020.08.012
更新日期:2020-12-01 00:00:00
abstract:INTRODUCTION:Type 2 diabetes mellitus (T2DM) patients have a variable response profile to the P2Y(12) receptor antagonist clopidogrel. P2Y(12) receptor signalling promotes platelet procoagulant activity. The aim of this study was to determine if T2DM patients with suboptimal clopidogrel response have greater platelet p...
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pub_type: 杂志文章
doi:10.1016/j.thromres.2008.10.001
更新日期:2009-07-01 00:00:00
abstract::Lipoprotein-associated coagulation inhibitor (LACI) is a plasma-derived protein that inhibits the tissue factor/factor VIIa/calcium/phospholipid complex in a factor Xa-dependent manner. Recombinant LACI (rLACI) added exogenously to plasma prolonged the activated partial thromboplastin time (APTT) and prothrombin time ...
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pub_type: 杂志文章
doi:10.1016/0049-3848(91)90120-l
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pub_type: 信件
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更新日期:2021-01-16 00:00:00
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更新日期:2015-01-01 00:00:00
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章,meta分析
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更新日期:2010-06-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90096-u
更新日期:1990-05-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90222-x
更新日期:1990-12-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:A higher level of health insurance coverage may be related to better quality of care and outcomes. Whether insurance status is associated with anticoagulation quality and clinical outcomes in patients with venous thromboembolism (VTE) is unknown. METHODS:We studied 819 elderly patients treated with vitami...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.thromres.2018.11.011
更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0049-3848(96)00089-8
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
abstract::Endothelial cells in biopsied blood vessels from von Willebrand factor (vWf)-deficient Doberman pinscher dogs contain immunologically detectable vWf. These dogs and normal dogs were treated with DDAVP (0.6 microgram/kg) and epinephrine (0.5 microgram/kg/min for 30 minutes) and were exercised, using 5 different exercis...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90198-l
更新日期:1990-01-01 00:00:00
abstract::We provide a centralised thrombophilia screening service with sample transfer by courier or first class mail, a practice common to many centres. Sample quality is of prime importance, thus we have assessed the effect of delayed sample handling upon the haemostatic variables within our thrombophilia profile. This compr...
journal_title:Thrombosis research
pub_type: 杂志文章
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更新日期:1997-09-15 00:00:00
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pub_type: 杂志文章
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更新日期:1994-07-15 00:00:00