Abstract：
:Bovine serum albumin (BSA, pI 4.9) was maleylated to yield highly anionic MBSA (pI 3.0). Maleylation of BSA lead to an expansion of molecular size of native BSA from an effective molecular radius (EMR) of 37 A to 57 A for MBSA as assessed by gel filtration chromatography. MBSA, but not BSA, bound to the peripheral capillary wall (PCW) and mesangium in vitro in frozen sections, and in vivo following i.v. injection (0.006 mg/g body wt), examined by immunofluorescence. When similarly injected rats or controls were given antibodies to either MBSA or BSA following injection of antigen, immune complexes were observed in glomeruli by immunofluorescence and EM only in MBSA injected rats. Deposits occurred in the mesangium and subendothelium in the PCW. In frozen sections, bound MBSA could be partially removed from tissue sections by high ionic strength buffer. Also, binding of MBSA was diminished by prior treatment of sections with synthetic polyanions. Maleylated bovine gamma-globulin and succinylated BSA showed identical binding patterns as described for MBSA, indicating that binding was not unique to the modified BSA molecule nor to the form of anionization. These results indicate that charge interactions between circulating highly anionic macromolecules and cationic domains within glomerular structures are responsible, in part, for MBSA binding and subsequent localization of immune complexes. Furthermore, it is inferred that the selective binding of MBSA to glomeruli and formation of immune complexes occurred by a mechanism not related to difference in size between MBSA and BSA. These findings are different from conventionally understood charge interactions in glomerular immune complex formation.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Barnes JL,Reznicek MJ,Radnik RA,Venkatachalam MAdoi
10.1038/ki.1988.161subject
Has Abstractpub_date
1988-08-01 00:00:00pages
156-63issue
2eissn
0085-2538issn
1523-1755pii
S0085-2538(15)34334-9journal_volume
34pub_type
杂志文章abstract：:The hormonal responsiveness profile of the cortical collecting duct varies from one species to another. To identify the hormones and agonists that modulate the functions of this tubule segment in the human species, we generated a cell line (HCD) immortalized by SV40 virus. The tubular origin of this cell line was asse...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.186
更新日期：1995-05-01 00:00:00
abstract：:Hypoalbuminemia is the most powerful predictor of mortality in end-stage renal disease. Since protein-calorie malnutrition can decrease albumin synthesis it is assumed that hypoalbuminemia results principally from malnutrition in these patients, but albumin synthesis may also be decreased as part of the acute-phase re...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.321
更新日期：1995-08-01 00:00:00
abstract：:When the endotoxin-lipopolysaccharide (LPS) derived from the cell wall of gram-negative bacteria is given to the rat in vivo, there are prompt, marked decreases in glomerular filtration rate (GFR), renal blood flow (RBF) and % Na+ reabsorption (%T-Na+). However, it has not been determined whether the endotoxin itself ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.105
更新日期：1990-05-01 00:00:00
abstract：:The nature of the light chains in mesangial IgA deposits and serum IgA was studied in patients with IgA nephropathy. Immunofluorescence (IF) studies using murine monoclonal antibodies, rabbit and goat anti-human monospecific antisera were performed in kidney sections from 15 IgA nephritic patients with only IgA isotyp...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.37
更新日期：1988-02-01 00:00:00
abstract：:A genome-wide association study (GWAS) indicated that myeloperoxidase-ANCA associated vasculitis (AAV) is associated with HLA-DQ. However, susceptibility alleles in these loci have been under-investigated. Here we genotyped 258 Chinese patients with myeloperoxidase-AAV and 597 healthy control individuals at HLA DRB1, ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2019.06.015
更新日期：2019-10-01 00:00:00
abstract：:Colony stimulating factor-1 (CSF-1) is a chemoattractant and growth factor for macrophages. In autoimmune MRL-lpr mice, CSF-1 is detected in the circulation and there is an increase in CSF-1 transcripts and macrophages in the kidney. The purpose of this study was to establish whether the MRL-lpr kidney is responsible ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.9
更新日期：1996-01-01 00:00:00
abstract：:This study was undertaken to test the hypothesis that, given equal arterial pressure reductions, the combination of an angiotensin converting enzyme (ACE) inhibitor and calcium antagonist slows declines in renal function and yields greater reductions in albuminuria over either agent alone. This hypothesis was evaluate...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/ki.1992.139
更新日期：1992-04-01 00:00:00
abstract：:Injury resulting from ischemia-reperfusion injury is a multifactorial process involving compromised function in both the tubular and the vascular compartments. Multiple vasoactive compounds have been implicated in the profound vasoconstriction that occurs in response to ischemia-reperfusion injury, and many of these f...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2011.145
更新日期：2011-08-01 00:00:00
abstract：UNLABELLED:Predicting renal survival in primary focal glomerulosclerosis from the time of presentation. BACKGROUND:To predict the risk of developing chronic renal failure in patients with primary focal glomerulosclerosis (FGS) using predictors available at the time of presentation, a retrospective analysis was perform...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.4491164
更新日期：1999-12-01 00:00:00
abstract：BACKGROUND:Altered mineral metabolism contributes to bone disease, cardiovascular disease, and other clinical problems in patients with end-stage renal disease. METHODS:This study describes the recent status, significant predictors, and potential consequences of abnormal mineral metabolism in representative groups of ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00185.x
更新日期：2005-03-01 00:00:00
abstract：:Effect of insulin-like growth factor-1 (IGF) on the synthesis of glomerular proteoglycans (PGs) in an ex vivo recirculating organ perfusion system was investigated. Kidneys were perfused with a medium (approximately 80 ml) containing [35S]-sulfate (250 microCi/ml) and IGF (62.5 to 625 ng/ml). After radiolabeling, a sm...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.188
更新日期：1992-05-01 00:00:00
abstract：:The renal 11beta-hydroxysteroid dehydrogenase type 2 (11betaHSD2) enzyme inactivates 11-hydroxy steroids in the kidney, thus protecting the nonselective mineralocorticoid receptor (MR) from occupation by glucocorticoids. The gene is highly expressed in all sodium-transporting epithelia, but also in human placenta, pan...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1046/j.1523-1755.2000.00978.x
更新日期：2000-04-01 00:00:00
abstract：:beta 2-microglobulin-related amyloidosis (A beta 2M) represents a frequent complication in long-term dialysis patients. Although the pathogenetic mechanism has yet to be fully understood, it is known that amyloid fibrils usually consist of intact molecules of beta 2-microglobulin (beta 2m). Plasma proteinase inhibitor...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.368
更新日期：1992-10-01 00:00:00
abstract：BACKGROUND:The clinical demographics of chronic dialysis patients are changing worldwide. However, long-term data from regional dialysis registries have not yet been analyzed and reported. METHODS:The Okinawa Dialysis Study (OKIDS) registry included all chronic dialysis patients treated in Okinawa, Japan, since 1971. ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00147.x
更新日期：2002-02-01 00:00:00
abstract：:Dual renin-angiotensin aldosterone (RAAS) blockade is associated with higher risk of hyperkalemia and has not been shown, in any outcome trial of validated renal end points, that is, doubling of creatinine, time to dialysis, or death, to be superior over other approaches. It shows promise in advanced proteinuric nephr...
journal_title：Kidney international
pub_type： 评论,杂志文章,评审
doi：10.1038/ki.2010.226
更新日期：2010-09-01 00:00:00
abstract：:Chronic cyclosporine (CsA) nephrotoxicity is a major complication of heart, bone marrow, and renal transplantation, and is characterized in humans by striped interstitial fibrosis, tubular dilatation and atrophy, and hyalinization of hilar arterioles. This last feature is highly specific for cyclosporine injury and ha...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.311
更新日期：1995-08-01 00:00:00
abstract：:Pathologic calcification is a significant cause of increased morbidity and mortality in patients with chronic kidney disease. The precise mechanisms of ectopic calcification are not fully elucidated, but it is known to be caused by an imbalance of procalcific and anticalcific factors. In the chronic kidney disease pop...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2015.12.046
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:Normal human podocytes are terminally differentiated and quiescent cells. It is not known why podocytes fail to proliferate in response to most forms of injury. Proliferation is regulated by cell cycle proteins and their inhibitors. The Cip/Kip family of cyclin-dependent kinase (CDK) inhibitors (p21, p27, p5...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00213.x
更新日期：2000-08-01 00:00:00
abstract：:The impact of high-flux hemodialysis on clinical outcomes remains controversial. We have previously shown that in vivo transmigrated leukocytes from patients with low-flux bioincompatible hemodialysis have an impaired capacity to upregulate CD11b at the site of interstitial inflammation. In the present study, we inves...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002090
更新日期：2007-03-01 00:00:00
abstract：:Major features of a long-standing inflammation in the kidney are vascular proliferation, glomerulosclerosis, interstitial fibrosis and tubular atrophy, leading to a gradual deterioration of the renal function. In this study we have investigated the expression of B-type receptors for platelet-derived growth factor (PDG...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.306
更新日期：1989-12-01 00:00:00
abstract：BACKGROUND:Segmental glomerular necrosis has been described in the biopsy material in a minority of patients with idiopathic IgA nephropathy in the oldest studies on this disease, but this marker of active capillaritis has received little attention in the subsequent literature, and its significance and relevance for th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.059002682.x
更新日期：2001-02-01 00:00:00
abstract：:Initiation of dialysis is associated with increased morbidity and mortality. However, a question could be asked whether these events are caused by initiation of dialysis or whether dialysis serves as a trigger unveiling or worsening underlying preexisting cardiovascular comorbidities that could be pre-treated. ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2015.271
更新日期：2015-11-01 00:00:00
abstract：:The Executive Summary of the KDIGO Clinical Practice Guideline for the Care of Kidney Transplant Recipients is presented in this issue of Kidney International. This guideline will require careful attention for implementation in the context of available resources within each country, especially those with underdevelope...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2009.468
更新日期：2010-02-01 00:00:00
abstract：:Hyperinsulinemia can contribute to hypertension through effects on sodium transport. To test whether the stimulatory effect of insulin on renal proximal tubule sodium transport is preserved in insulin resistance, we compared the effects of insulin on abdominal adipocytes and proximal tubules in rats and humans. Insuli...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2014.351
更新日期：2015-03-01 00:00:00
abstract：:Amylin (islet amyloid peptide) plays a critical role in islet amyloidosis and in the development of beta-cell dysfunction in patients with diabetes; however, the involvement of amylin in renal amyloidosis has not been studied. For this reason, we surveyed 149 patients with biopsy-proven diabetic nephropathy (DN). The ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002305
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND:Tacrolimus is a relatively new calcineurin inhibitor that has been increasingly used in transplant medicine. The objective of the current work is to report our preliminary experience with tacrolimus in the treatment of diffuse proliferative glomerulonephritis in systemic lupus erythematosus (SLE). METHODS:N...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1111/j.1523-1755.2005.00461.x
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND:Recent clinical trials of mycophenolate mofetil (MMF) in chronic allograft nephropathy (CAN) demonstrated that the dose of cyclosporine A (CsA) is one of the critical factors in determining graft function in CAN, but the effect of MMF on chronic CsA nephropathy is undetermined. We undertook this study to eva...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00400.x
更新日期：2002-07-01 00:00:00
abstract：:Neutrophil gelatinase-associated lipocalin (Ngal, 24p3, SIP24, lipocalin 2, or siderocalin) was originally purified from neutrophils, but with unknown function. Recently, it was identified that Ngal activates nephron formation in the embryonic kidney, is rapidly and massively induced in renal failure and possesses kid...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002165
更新日期：2007-05-01 00:00:00
abstract：:Transforming growth factor-β1 (TGF-β1) upregulation occurs in virtually all chronic kidney diseases and is associated with podocyte injury and proteinuria; however, the mechanisms contributing to this in vivo are ambiguous. In vitro, incubation of podocytes with TGF-β1 induced Wnt1 expression, β-catenin activation, an...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.255
更新日期：2011-12-01 00:00:00
abstract：:Fibroblast growth factor-23 (FGF23), a regulator of mineral metabolism, has been linked to cardiovascular disease in chronic kidney disease. As community-based data of the longitudinal association between FGF23 and cardiovascular events are lacking, we investigated a possible relationship in 727 men of the Uppsala Lon...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.327
更新日期：2013-01-01 00:00:00
