Role of adenosine on glucagon-induced cAMP in a human cortical collecting duct cell line.


:The hormonal responsiveness profile of the cortical collecting duct varies from one species to another. To identify the hormones and agonists that modulate the functions of this tubule segment in the human species, we generated a cell line (HCD) immortalized by SV40 virus. The tubular origin of this cell line was assessed by the expression of collecting duct-specific antigens and the ability of vasopressin to increase by nine-fold cAMP synthesis. Glucagon and adenosine stimulated cAMP synthesis, and atrial natriuretic peptide stimulated cGMP synthesis in a concentration-dependent manner. Bradykinin, adenosine and angiotensin increased intracellular calcium concentration ([Ca2+]i). Because adenosine can regulate tubular functions, we examined its role on glucagon-induced cAMP synthesis. Using adenosine analogs, we demonstrated that HCT cells both expressed adenosine type-2 (A2) receptors which stimulated cAMP production, and adenosine type-1 (A1) receptors linked to [Ca2+]i increase which inhibited glucagon-stimulated cAMP synthesis. The inhibitory effect was abolished by pertussis toxin, and was neither due to [Ca2+]i increase nor to protein kinase C activation, which indicated that some A1 adenosine receptors were directly negatively coupled to adenylyl cyclase. These results suggest that adenosine can modify human cortical collecting duct functions in opposite ways according to the adenosine receptor activated.


Kidney Int


Kidney international


Prié D,Friedlander G,Coureau C,Vandewalle A,Cassingéna R,Ronco PM




Has Abstract


1995-05-01 00:00:00














  • Dietary NaCl-restriction prevents the calciuria of KCl-deprivation and blunts the calciuria of KHCO3-deprivation in healthy adults.

    abstract::Previous studies have demonstrated that dietary potassium deprivation in healthy human subjects eating diets otherwise containing normal quantities of NaCl is accompanied by an increase in urinary calcium excretion. This increase in urinary Ca excretion occurs in association with reductions in urinary Na and Cl excret...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章


    authors: Lemann J Jr,Pleuss JA,Hornick L,Hoffman RG

    更新日期:1995-03-01 00:00:00

  • Impaired renal acidification following acute renal ischemia in the dog.

    abstract::Transient renal tubular acidosis may complicate acute renal failure (ARF). To clarify this phenomenon, the present study examined tubular H+ ion secretory capacity in an ischemic model of ARF. Clearance studies were performed in dogs subjected to 60 minutes, unilateral renal artery clamping. The contralateral kidney s...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Winaver J,Agmon D,Harari R,Better OS

    更新日期:1986-12-01 00:00:00

  • Urine citrate excretion as a marker of acid retention in patients with chronic kidney disease without overt metabolic acidosis.

    abstract::Acid (H+) retention appears to contribute to progressive decline in glomerular filtration rate (GFR) in patients with chronic kidney disease (CKD), including some patients without metabolic acidosis. Identification of patients with H+ retention but without metabolic acidosis could facilitate targeted alkali therapy; h...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Goraya N,Simoni J,Sager LN,Madias NE,Wesson DE

    更新日期:2019-05-01 00:00:00

  • The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity.

    abstract::The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Martuseviciene G,Hofman-Bang J,Clausen T,Olgaard K,Lewin E

    更新日期:2011-04-01 00:00:00

  • Treatment of experimental renal osteodystrophy with pamidronate.

    abstract::We evaluated the effects of the bisphosphonate pamidronate on bone histomorphometry, structure and strength in male rats with uninephrectomy or with chronic renal disease induced by 5/6 nephrectomy. In rats with chronic renal disease the plasma urea, phosphate and parathyroid hormone levels were significantly increase...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Jokihaara J,Pörsti IH,Kööbi P,Jolma PM,Mustonen JT,Saha HH,Sievänen H,Kannus P,Iwaniec UT,Turner RT,Järvinen TL

    更新日期:2008-08-01 00:00:00

  • Metabolic acidosis and skeletal muscle adaptation to low protein diets in chronic uremia.

    abstract::To maintain nitrogen equilibrium when prescribed a low protein diet (LPD), metabolic adaptations occur involving a reduction protein turnover, principally decreased muscle protein degradation. Studies suggest that in patients with chronic renal failure (CRF) uncomplicated by metabolic acidosis (MA), these adaptive res...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Williams B,Hattersley J,Layward E,Walls J

    更新日期:1991-10-01 00:00:00

  • Prospective assessment of antidonor cellular alloreactivity is a tool for guidance of immunosuppression in kidney transplantation.

    abstract::Current characterization of the immune risk in renal transplant patients is only focused on the assessment of preformed circulating alloantibodies; however, alloreactive memory T cells are key players in mediating allograft rejection. Immune monitoring of antidonor alloreactive memory/effector T cells using an IFN-γ E...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Bestard O,Cruzado JM,Lucia M,Crespo E,Casis L,Sawitzki B,Vogt K,Cantarell C,Torras J,Melilli E,Mast R,Martinez-Castelao A,Gomà M,Reinke P,Volk HD,Grinyó JM

    更新日期:2013-12-01 00:00:00

  • Cardiovascular disease in pediatric chronic dialysis patients.

    abstract:BACKGROUND:Little information is available regarding cardiac morbidity and mortality in children with end-stage renal disease. We sought to determine the incidence of cardiac morbidity and mortality in pediatric chronic dialysis patients. METHODS:Medicare incident pediatric (0 to 19 years) dialysis patients from 1991 ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Chavers BM,Li S,Collins AJ,Herzog CA

    更新日期:2002-08-01 00:00:00

  • Glutathione S-transferase polymorphisms and skin cancer after renal transplantation.

    abstract:BACKGROUND:Susceptibility to skin cancer after transplantation is multifactorial, and risk factors include skin type, sun exposure, and level of immunosuppression. A major mechanism of carcinogenesis is ultraviolet radiation-induced free radical damage, and genetically determined ability to metabolize free radicals may...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Marshall SE,Bordea C,Haldar NA,Mullighan CG,Wojnarowska F,Morris PJ,Welsh KI

    更新日期:2000-11-01 00:00:00

  • Plasminogen activator inhibitor-1 expression is regulated by the angiotensin type 1 receptor in vivo.

    abstract:BACKGROUND:The fibrinolytic system plays an important role in degrading fibrin-rich thrombi and in vascular and tissue remodeling. Elevated levels of plasminogen activator inhibitor-1 (PAI-1) can reduce the efficiency of the endogenous fibrinolytic system. Angiotensin (Ang) has been shown to regulate PAI-1 expression v...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Nakamura S,Nakamura I,Ma L,Vaughan DE,Fogo AB

    更新日期:2000-07-01 00:00:00

  • Changes in cancer incidence and outcomes among kidney transplant recipients in the United States over a thirty-year period.

    abstract::Recipients of kidney transplants have elevated cancer risk compared with the general population. Improvements over time in transplant care and cancer treatment may have affected incidence and outcomes of cancer among recipients of kidney transplant. To evaluate this, we used linked United States transplant and cancer ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Blosser CD,Haber G,Engels EA

    更新日期:2020-11-04 00:00:00

  • Nitric oxide mediates cyclosporine-induced apoptosis in cultured renal cells.

    abstract:BACKGROUND:The clinical use of cyclosporine (CsA) is limited by its nephrotoxicity. Apoptosis, perhaps instigated by increased nitric oxide synthase (NOS) activity, may play a role in such toxicity. METHODS:Human mesangial cells, human tubular cells, human umbilical vein endothelial cells, or murine endothelial cells ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Amore A,Emancipator SN,Cirina P,Conti G,Ricotti E,Bagheri N,Coppo R

    更新日期:2000-04-01 00:00:00

  • Prevalence, pathogenesis, and functional significance of aldosterone deficiency in hyperkalemic patients with chronic renal insufficiency.

    abstract::Our findings indicate that hypoaldosteronism occurs commonly (23/31 patients) in hyperkalemic patients with chronic renal insufficiency and that the deficiency of aldosterone contributes to the pathogenesis of the hyperkalemia. In most patients (83%), hypoaldosteronism could be accounted for by deficient renal secreti...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Schambelan M,Sebastian A,Biglieri EG

    更新日期:1980-01-01 00:00:00

  • Influence of systemically applied angiotensin II on the microcirculation of glomerular capillaries in the rat.

    abstract::The effect of intravenous infusion of angiotensin II on microvascular parameters of the renal microcirculation of rats was studied. With the aid of fluorescence microscopy and a high sensitivity video system we observed the passage of fluorescence-labeled erythrocytes through single glomerular capillaries on the surfa...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Zimmerhackl B,Parekh N,Kücherer H,Steinhausen M

    更新日期:1985-01-01 00:00:00

  • Studies of renal autoregulation in pancreatectomized and streptozotocin diabetic rats.

    abstract::We studied renal autoregulation in pancreatectomized Munich-Wistar diabetic rats and in their sham-operated controls. In a second experiment we studied renal autoregulation in untreated and insulin treated streptozotocin diabetic Munich-Wistar rats and their nondiabetic controls. In the first experiment the diabetic r...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Mauer SM,Brown DM,Steffes MW,Azar S

    更新日期:1990-03-01 00:00:00

  • Biocompatible peritoneal dialysis solutions: many questions but few answers.

    abstract::The clinical value of biocompatible peritoneal dialysis solutions is uncertain because of inconsistent findings in randomized controlled trials. A systematic review by Cho et al. examining 20 such trials suggests a beneficial effect on residual renal function. However, the finding is not robust and may relate to decre...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Blake PG,Jain AK,Yohanna S

    更新日期:2013-11-01 00:00:00

  • Mast cell activation and degranulation promotes renal fibrosis in experimental unilateral ureteric obstruction.

    abstract::Progressive renal fibrosis is the final common pathway leading to renal failure irrespective of the initiating cause. Clinical studies of renal fibrosis found that prominent mast cell accumulation correlated with worse outcomes. Mast cells are pluripotent innate immune cells that synthesize and secrete profibrotic med...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Summers SA,Gan PY,Dewage L,Ma FT,Ooi JD,O'Sullivan KM,Nikolic-Paterson DJ,Kitching AR,Holdsworth SR

    更新日期:2012-09-01 00:00:00

  • Screening early renal failure: cut-off values for serum creatinine as an indicator of renal impairment.

    abstract:BACKGROUND:The aim of this study was to define cut-off values for serum creatinine as an indicator of several levels of renal impairment. METHODS:To identify the suitable values, receiver operating characteristic curves were constructed based on the data of 984 laboratory assessments of renal function. The glomerular ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Couchoud C,Pozet N,Labeeuw M,Pouteil-Noble C

    更新日期:1999-05-01 00:00:00

  • Global trends in the rates of living kidney donation.

    abstract::As the worldwide prevalence of end-stage renal disease increases it is important to evaluate the rate of living kidney donation in various countries; however there is no comprehensive global assessment of these rates. To measure this, we compiled data from representatives, renal registries, transplant networks, publis...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Horvat LD,Shariff SZ,Garg AX,Donor Nephrectomy Outcomes Research (DONOR) Network.

    更新日期:2009-05-01 00:00:00

  • Alloreactive T cells and 'the persistence of memory'.

    abstract::Circulating donor-reactive effector memory T cells have been associated with allograft injury in humans. The next step to translate this observation into clinical practice is the design of clinical trials aimed at individualization of T cell-targeted therapy. The administration of immunosuppressive therapy based on th...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Poggio ED,Augustine JJ

    更新日期:2013-12-01 00:00:00

  • Urinary kallikrein and salt sensitivity in essential hypertensive males.

    abstract::A strong influence of urinary kallikrein excretion on the salt sensitivity of blood pressure has been recently suggested in normotensive patients. To evaluate the relationship between kallikrein and salt sensitivity in essential hypertension, active kallikrein excretion, plasma renin activity, atrial natriuretic pepti...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Ferri C,Bellini C,Carlomagno A,Perrone A,Santucci A

    更新日期:1994-09-01 00:00:00

  • Acidosis and hypoxic medullary injury in the isolated perfused kidney.

    abstract::The effects of acidosis on renal function and morphology were examined in the isolated perfused rat kidney (IPK). Kidneys were perfused with oxygenated Krebs-Henseleit-albumin medium for 60 minutes at pH 7.4 or pH 7.0. At the lower pH, GFR was reduced by 25%, TRNa by 32% and oxygen consumption by 41% as compared to pe...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Shanley PF,Shapiro JI,Chan L,Burke TJ,Johnson GC

    更新日期:1988-12-01 00:00:00

  • Protection from renal ischemia-reperfusion injury by the 2-methylaminochroman U83836E.

    abstract:BACKGROUND:In a prior study the 21-aminosteroid (lazaroid) U74389F provided in vivo protection from oxidative stress when used as a preventive therapy in ischemia-reperfusion injury in the kidney. As the cell membrane is the principal site for lipoperoxidation, in the current study the very lipophilic 2-methylaminochro...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: De Vecchi E,Lubatti L,Beretta C,Ferrero S,Rinaldi P,Galli Kienle M,Trazzi R,Paroni R

    更新日期:1998-09-01 00:00:00

  • Genetic susceptibility to severe course of nephropathia epidemica caused by Puumala hantavirus.

    abstract::Nephropathia epidemica (NE) caused by Puumala hantavirus is one type of hemorrhagic fever with renal syndrome (HFRS). There is considerable variability in the clinical severity of NE. Many infections are subclinical but the disease can even be fatal. We questioned whether the wide spectrum in the outcome of NE is depe...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Mustonen J,Partanen J,Kanerva M,Pietilä K,Vapalahti O,Pasternack A,Vaheri A

    更新日期:1996-01-01 00:00:00

  • Proinflammatory gene expression and macrophage recruitment in the rat remnant kidney.

    abstract:BACKGROUND:Macrophage (Mphi) infiltration may contribute to chronic renal injury. We therefore sought to examine the expression of genes associated with Mphi recruitment in the rat remnant kidney model. METHODS:Male Munich Wistar rats underwent 5/6 nephrectomy or sham operation (SHM, N = 18) and received no treatment ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Taal MW,Zandi-Nejad K,Weening B,Shahsafaei A,Kato S,Lee KW,Ziai F,Jiang T,Brenner BM,MacKenzie HS

    更新日期:2000-10-01 00:00:00

  • Accumulation of protein-bound uremic toxins: the kidney remains the leading culprit in the gut-liver-kidney axis.

    abstract::Protein-bound uremic toxins (PBUTs) accumulate in chronic kidney disease (CKD) and are poorly removed by dialysis. Gryp et al. demonstrated that fecal bacteria from patients with CKD do not produce more PBUTs than do those from healthy controls and that the accumulation of PBUTs, as CKD progresses, is mainly due to th...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Jourde-Chiche N,Burtey S

    更新日期:2020-06-01 00:00:00

  • Role of vascular endothelial growth factor in the regulation of angiogenesis.

    abstract::Compelling evidence indicates that vascular endothelial growth factor (VEGF) is a fundamental regulator of normal and abnormal angiogenesis. The loss of a single VEGF allele results in defective vascularization and early embryonic lethality. VEGF plays also a critical role in kidney development, and its inactivation d...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Ferrara N

    更新日期:1999-09-01 00:00:00

  • Effect of iron status on the intestinal absorption of aluminum: a reappraisal.

    abstract::Clinical and experimental studies have shown that serum aluminum (Al) is bound to transferrin and that cellular uptake of Al appears to be mediated by transferrin receptors. Based on these findings it is widely believed that intestinal Al absorption occurs via iron-specific, transferrin-dependent pathways and that iro...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Ittel TH,Kinzel S,Ortmanns A,Sieberth HG

    更新日期:1996-12-01 00:00:00

  • Induction of manganese superoxide dismutase by glucocorticoids in glomerular cells.

    abstract::Our previous in vivo study demonstrated that methylprednisolone (MP) activates glomerular antioxidant enzymes and attenuates glomerular oxidant injuries, including those in experimental nephrosis. The present study investigates the cellular mechanism of the MP-induced activation of antioxidant enzymes and their contri...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Yoshioka T,Kawamura T,Meyrick BO,Beckman JK,Hoover RL,Yoshida H,Ichikawa I

    更新日期:1994-01-01 00:00:00

  • Association of APOL1 variants with mild kidney disease in the first-degree relatives of African American patients with non-diabetic end-stage renal disease.

    abstract::Familial aggregation of non-diabetic end-stage renal disease (ESRD) is found in African Americans and variants in the apolipoprotein L1 gene (APOL1) contribute to this risk. To detect genetic associations with milder forms of nephropathy in the high-risk families, analyses were performed using generalized estimating e...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Freedman BI,Langefeld CD,Turner J,Núñez M,High KP,Spainhour M,Hicks PJ,Bowden DW,Reeves-Daniel AM,Murea M,Rocco MV,Divers J

    更新日期:2012-10-01 00:00:00