Abstract:
BACKGROUND:The parasitic mite Varroa destructor is considered the major pest of the European honey bee (Apis mellifera) and responsible for declines in honey bee populations worldwide. Exploiting the full potential of gene sequences becoming available for V. destructor requires adaptation of modern molecular biology approaches to this non-model organism. Using a mu-class glutathione S-transferase (VdGST-mu1) as a candidate gene we investigated the feasibility of gene knockdown in V. destructor by double-stranded RNA-interference (dsRNAi). RESULTS:Intra-haemocoelic injection of dsRNA-VdGST-mu1 resulted in 97% reduction in VdGST-mu1 transcript levels 48 h post-injection compared to mites injected with a bolus of irrelevant dsRNA (LacZ). This gene suppression was maintained to, at least, 72 h. Total GST catalytic activity was reduced by 54% in VdGST-mu1 gene knockdown mites demonstrating the knockdown was effective at the translation step as well as the transcription steps. Although near total gene knockdown was achieved by intra-haemocoelic injection, only half of such treated mites survived this traumatic method of dsRNA administration and less invasive methods were assessed. V. destructor immersed overnight in 0.9% NaCl solution containing dsRNA exhibited excellent reduction in VdGST-mu1 transcript levels (87% compared to mites immersed in dsRNA-LacZ). Importantly, mites undergoing the immersion approach had greatly improved survival (75-80%) over 72 h, approaching that of mites not undergoing any treatment. CONCLUSIONS:Our findings on V. destructor are the first report of gene knockdown in any mite species and demonstrate that the small size of such organisms is not a major impediment to applying gene knockdown approaches to the study of such parasitic pests. The immersion in dsRNA solution method provides an easy, inexpensive, relatively high throughput method of gene silencing suitable for studies in V. destructor, other small mites and immature stages of ticks.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Campbell EM,Budge GE,Bowman ASdoi
10.1186/1756-3305-3-73subject
Has Abstractpub_date
2010-08-16 00:00:00pages
73issn
1756-3305pii
1756-3305-3-73journal_volume
3pub_type
杂志文章abstract:BACKGROUND:Multiplicity and genetic diversity of Plasmodium falciparum infection might play a role in determining the clinical outcome of malaria infection and could be a fair reflection of the disease transmission rate. This study investigated the genetic diversity of P. falciparum and multiplicity of infection in rel...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1641-z
更新日期:2016-06-27 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2020-09-10 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3381-3
更新日期:2019-03-19 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3873-1
更新日期:2020-01-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0783-8
更新日期:2015-03-23 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1943-1
更新日期:2017-01-03 00:00:00
abstract:: The proteasome inhibitor and anti-cancer drug bortezomib was tested for in vitro activity against bloodstream forms of Trypanosoma brucei. The concentrations of bortezomib required to reduce the growth rate by 50% and to kill all trypanosomes were 3.3 nM and 10 nM, respectively. In addition, bortezomib was 10 times m...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2009-07-07 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3105-0
更新日期:2018-09-29 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04263-3
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-76
更新日期:2010-08-24 00:00:00
abstract::Echinococcus transmission is known to be affected by various environmental factors, which may be modified by human influence or natural events including global warming. Considerable population growth in the last fifty years in Ningxia Hui Autonomous Region (NHAR), the People's Republic of China (PRC), has led to drama...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
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更新日期:2012-07-24 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3301-6
更新日期:2019-01-15 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2556-z
更新日期:2017-12-27 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2742-7
更新日期:2018-03-07 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2014-12-03 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1203-9
更新日期:2015-11-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2014-11-20 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2011-05-26 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1198-2
更新日期:2015-11-10 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1066-0
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:Toxoplasmosis is an important zoonosis, being a cause of congenital disease and abortion in animals and humans. DNA vaccination as a promising vaccine remains a challenge for an improved delivery system. METHODS:In this study, attenuated Salmonella typhimurium BRD509 was used to deliver a DNA vaccine encodi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-145
更新日期:2014-03-31 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1331-x
更新日期:2016-01-28 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2815-7
更新日期:2018-04-13 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-104
更新日期:2013-04-16 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1854-1
更新日期:2016-11-02 00:00:00
abstract:BACKGROUND:Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the m...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-223
更新日期:2014-05-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1035-7
更新日期:2015-08-16 00:00:00