Abstract：
BACKGROUND:Toxoplasma gondii, an obligate intracellular protozoan parasite, possesses the remarkable ability to co-opt host cell machinery in order to maintain its intracellular survival. This parasite can modulate signaling pathways of its host through the secretion of polymorphic effector proteins localized in the rhoptry and dense granule organelles. One of such effectors is T. gondii type II-specific dense granule protein 15, TgGRA15, which activates NF-κB pathway. The aim of the present study was to identify the host interaction partner proteins of TgGRA15. METHODS:We screened a yeast two-hybrid mouse cDNA library using TgGRA15 as the bait. TgGRA15 (PRU strain, Type II) was cloned into the pGBKT7 vector and expressed in the Y2HGold yeast strain. Then, the bait protein expression was validated by western blotting analysis, followed by auto-activation and toxicity tests in comparison with control (Y2HGold yeast strain transformed with empty pGBKT7 vector). RESULTS:This screening led to the identification of mouse Luzp1 and AW209491 as host binding proteins that interact with TgGRA15. Luzp1 contains three nuclear localizing signals and is involved in regulating a subset of host non-coding RNA genes. CONCLUSIONS:These findings reveal, for the first time, new host cell proteins interacting with TgGRA15. The identification of these cellular targets and the understanding of their contribution to the host-pathogen interaction may serve as the foundation for novel therapeutic and prevention strategies against T. gondii infection.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Liu Q,Li FC,Elsheikha HM,Sun MM,Zhu XQdoi
10.1186/s13071-016-1943-1subject
Has Abstractpub_date
2017-01-03 00:00:00pages
1issue
1issn
1756-3305pii
10.1186/s13071-016-1943-1journal_volume
10pub_type
杂志文章abstract：BACKGROUND:Correct species identification of blow flies is a crucial step for understanding their biology, which can be used not only for designing fly control programs, but also to determine the minimum time since death. Identification techniques are usually based on morphological and molecular characters. However, th...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2163-z
更新日期：2017-05-10 00:00:00
abstract：BACKGROUND:Sarcoptic mange causes significant animal welfare and occasional conservation concerns for bare-nosed wombats (Vombatus ursinus) throughout their range. To date, in situ chemotherapeutic interventions have involved macrocytic lactones, but their short duration of action and need for frequent re-administratio...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04500-9
更新日期：2021-01-06 00:00:00
abstract：BACKGROUND:Blackflies (Diptera: Simuliidae) are known as effective vectors of human and animal pathogens, worldwide. We have already indicated that some individuals in the Simulium turgaicum complex are annoying pests of humans and livestock in the Aras River Basin, Iran. However, there is no evidence of host preferenc...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04432-4
更新日期：2020-11-04 00:00:00
abstract：BACKGROUND:Ixodes ricinus, a competent vector of several pathogens, is the tick species most frequently reported to bite humans in Europe. The majority of human cases of Lyme borreliosis (LB) and tick-borne encephalitis (TBE) occur in the north-eastern region of Italy. The aims of this study were to detect the occurren...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-61
更新日期：2012-03-27 00:00:00
abstract：BACKGROUND:The World Health Organization (WHO) recommends for sub-Saharan Africa a package of prompt and effective case-management combined with the delivery of insecticide-treated nets (ITN) and intermittent preventive treatment during pregnancy (IPTp) with sulphadoxine-pyrimethamine (SP) through the national antenata...
journal_title：Parasites & vectors
pub_type： 杂志文章,多中心研究
doi：10.1186/s13071-014-0495-5
更新日期：2014-11-20 00:00:00
abstract：BACKGROUND:Japan is one of the few countries believed to have eliminated soil-transmitted helminths (STHs). In 1949, the national prevalence of Ascaris lumbricoides was 62.9%, which decreased to 0.6% in 1973 due to improvements in infrastructure, socioeconomic status, and the implementation of national STH control meas...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3875-z
更新日期：2020-01-08 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章,随机对照试验
doi：10.1186/1756-3305-6-3
更新日期：2013-01-04 00:00:00
abstract：BACKGROUND:Both falciparum and vivax malaria were historically prevalent in China with high incidence. With the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000. Despite this, the re-emergence or outbreak of malaria was unavoidable...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-136
更新日期：2012-07-09 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-26
更新日期：2010-04-08 00:00:00
abstract：BACKGROUND:Infections by protozoans of the genus Giardia are a common cause of diarrhea in dogs. Canine giardiosis constitutes a disease with a zoonotic potential; however, it is often underestimated due to its challenging diagnosis. The objective of the study was to assess the diagnostic performance of an immunochroma...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04422-6
更新日期：2020-11-02 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3401-3
更新日期：2019-03-25 00:00:00
abstract：BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0608-1
更新日期：2015-01-06 00:00:00
abstract：BACKGROUND:Spirocerca lupi is a parasitic nematode of canids that can lead to a severe and potentially fatal disease. Recently, a new species, Spirocerca vulpis, was described from red foxes in Europe, suggesting a high genetic diversity of the Spirocerca spp. infecting canids. The genetic variation and phylogenetic re...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-3202-0
更新日期：2018-12-14 00:00:00
abstract：BACKGROUND:In this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spec...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2597-3
更新日期：2018-01-04 00:00:00
abstract：BACKGROUND:Ixodes ricinus is the most common tick species in Europe and the main vector for Borrelia burgdorferi (sensu lato) and tick-borne encephalitis virus (TBEV). It is involved also in the transmission of Borrelia miyamotoi, a relapsing fever spirochete that causes health disorders in humans. Little is known rega...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-3969-7
更新日期：2020-02-27 00:00:00
abstract：BACKGROUND:The trypanosome diversity of the Brush-tailed Bettong (Bettongia penicillata), known locally as the woylie, has been further investigated. At a species level, woylies are critically endangered and have declined by 90% since 1999. The predation of individuals made more vulnerable by disease is thought to be t...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-121
更新日期：2013-04-26 00:00:00
abstract：BACKGROUND:Feline babesiosis, sporadically reported from various countries, is of major clinical significance in South Africa, particularly in certain coastal areas. Babesia felis, B. leo, B. lengau and B. microti have been reported from domestic cats in South Africa. Blood specimens from domestic cats (n = 18) showing...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3395-x
更新日期：2019-03-26 00:00:00
abstract：BACKGROUND:Resistance to chemical insecticides plus high morbidity rates have lead to rising interest in fungi as candidates for biocontrol agents of mosquito vectors. In most studies fungal infections have been induced by exposure of mosquitoes to various surfaces treated with conidia. In the present study eight Mexic...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-24
更新日期：2011-02-26 00:00:00
abstract：BACKGROUND:Dengue virus (DENV) is the arbovirus with the highest incidence in New Caledonia and in the South Pacific region. In 2012-2014, a major DENV-1 outbreak occurred in New Caledonia. The only known vector of DENV in New Caledonia is Aedes aegypti but no study has yet evaluated the competence of New Caledonia Ae....
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2319-x
更新日期：2017-08-09 00:00:00
abstract：:Presently, 45% of the total human population of Europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (VBH) causing diseases. A plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly ...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/1756-3305-6-16
更新日期：2013-01-16 00:00:00
abstract：BACKGROUND:Polovodova method based on counting follicular dilatations estimates the number of egg-laying in mosquitoes. However, some researchers doubt the reliability of this method because of the absence of multiple dilatations in vectors after many gonotrophic cycles. It is in this context of controversy that our st...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-0731-7
更新日期：2015-02-22 00:00:00
abstract：:Companion vector-borne diseases (CVBDs) are an important threat for pet life, but may also have an impact on human health, due to their often zoonotic character. The importance and awareness of CVBDs continuously increased during the last years. However, information on their occurrence is often limited in several part...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/s13071-019-3407-x
更新日期：2019-03-28 00:00:00
abstract：BACKGROUND:Even though male-biased parasitism is common in mammals, little effort has been made to evaluate whether higher parasitic burden in males results in an extra biological cost, and thus a decrease in fitness. Body condition impairment and the augmentation of oxidative stress can be used as indicators of the co...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2060-5
更新日期：2017-04-21 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2051-6
更新日期：2017-03-13 00:00:00
abstract：BACKGROUND:Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection. The prevalence and intensity of schistosomiasis inf...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04420-8
更新日期：2020-10-27 00:00:00
abstract：BACKGROUND:Live attenuated Leishmania donovani parasites as LdCen(-/-) were shown to confer protective immunity against Leishmania infection in mice, hamsters, and dogs. Strong immunogenicity in dogs vaccinated with LdCen(-/-) has been previously reported, including increased antibody response favoring Th1 response lym...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1528-z
更新日期：2016-04-30 00:00:00
abstract：BACKGROUND:A good understanding of mosquito ecology is imperative for integrated vector control of malaria. In breeding sites, Anopheles larvae are concurrently exposed to predators and parasites. However, to our knowledge, there is no study on combined effects of predators and parasites on development and survival of ...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/s13071-020-3915-8
更新日期：2020-01-30 00:00:00
abstract：BACKGROUND:Zoonotic leishmaniosis, caused by the protozoan Leishmania infantum, is a public and animal health problem in Asia, Central and South America, the Middle East and the Mediterranean Basin. Several phlebotomine sand fly species from the subgenus Larroussius are vectors of L. infantum. Data from dogs living in ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3376-0
更新日期：2019-03-26 00:00:00
abstract：BACKGROUND:The Cqm1 α-glucosidase of Culex quinquefasciatus larvae acts as the midgut receptor for the binary toxin of the biolarvicide Lysinibacillus sphaericus. Mutations within the cqm1 gene can code for aberrant polypeptides that can no longer be properly expressed or bind to the toxin, leading to insect resistance...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1347-2
更新日期：2016-02-04 00:00:00
abstract：BACKGROUND:In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, B...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1963-x
更新日期：2017-01-24 00:00:00
