Abstract:
BACKGROUND:Live attenuated Leishmania donovani parasites as LdCen(-/-) were shown to confer protective immunity against Leishmania infection in mice, hamsters, and dogs. Strong immunogenicity in dogs vaccinated with LdCen(-/-) has been previously reported, including increased antibody response favoring Th1 response lymphoproliferative responses, CD4(+) and CD8(+) T-cells activation, increased levels of Th1 and reduction of Th2 cytokines, in addition to a significant reduction in parasite burden after 18 and 24 months post virulent parasite challenge. METHODS:Aimed at validating a new method using in vitro co-culture systems with macrophages and purified CD4(+) or CD8(+) or CD4(+):CD8(+) T-cells of immunized dogs with both LdCen(-/-) and Leishmune® to assess microbicide capacity of macrophages and the immune response profile as the production of IFN-γ, TNF-α, IL-12, IL-4 and IL-10 cytokines. RESULTS AND DISCUSSION:Our data showed co-cultures of macrophages and purified T-cells from dogs immunized with LdCen(-/-) and challenged with L. infantum were able to identify high microbicidal activity, especially in the co-culture using CD4(+) T-cells, as compared to the Leishmune® group. Similarly, co-cultures with CD8(+) T-cells or CD4(+):CD8(+) T-cells in both experimental groups were able to detect a reduction in the parasite burden in L. infantum infected macrophages. Moreover, co-cultures using CD4(+) or CD8(+) or CD4(+):CD8(+) T-cells from immunized dogs with both LdCen(-/-) and Leishmune® were able to identify higher levels of IFN-γ and IL-12 cytokines, reduced levels of IL-4 and IL-10, and a higher IFN-γ/IL-10 ratio. While the highest IFN-γ levels and IFN-γ/IL-10 ratio were the hallmarks of LdCen(-/-) group in the co-culture using CD4(+) T-cells, resulting in strong reduction of parasitism, the Leishmune® immunization presented a differential production of TNF-α in the co-culture using CD4(+):CD8(+) T-cells. CONCLUSION:The distinct conditions of co-culture systems were validated and able to detect the induction of immune protection. The method described in this study applied a new, more accurate approach and was able to yield laboratory parameters useful to test and monitor the immunogenicity and efficacy of Leishmania vaccines in dogs.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Viana KF,Fiuza JA,Gannavaram S,Dey R,Selvapandiyan A,Bartholomeu DC,da Silveira-Lemos D,Bueno LL,Dutra WO,Fujiwara RT,Nakhasi HL,Giunchetti RCdoi
10.1186/s13071-016-1528-zsubject
Has Abstractpub_date
2016-04-30 00:00:00pages
250issn
1756-3305pii
10.1186/s13071-016-1528-zjournal_volume
9pub_type
杂志文章abstract::Taenia solium life cycle includes humans as definitive hosts and pigs as intermediate hosts. One of the measures to stop the life cycle of this parasite is by vaccination of pigs. In experiments performed in pigs with TSOL18 and TSOL45-1A, two recombinant T. solium proteins, 99.5% and 97.0% protection was induced, res...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-3
更新日期:2011-01-06 00:00:00
abstract:BACKGROUND:Malaria transmission in Cameroon is mediated by a plethora of vectors that are heterogeneously distributed across the country depending on the biotope. To effectively guide malaria control operations, regular update on the role of local Anopheles species is essential. Therefore, an entomological survey was c...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-262
更新日期:2014-06-05 00:00:00
abstract:BACKGROUND:Helminth neuroinfections represent a serious health problem, but host immune mechanisms in the nervous tissue often remain undiscovered. This study aims at in vitro characterization of the response of murine astrocytes and microglia exposed to Trichobilharzia regenti which is a neuropathogenic schistosome mi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1869-7
更新日期:2016-11-14 00:00:00
abstract:BACKGROUND:Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans, a non-invasive loop-mediated isothermal amplification (LAMP) assa...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0991-2
更新日期:2015-07-15 00:00:00
abstract:BACKGROUND:The use of phytochemicals is a promising solution in biological control against salmon louse (Lepeophtheirus salmonis). Glucosinolates belong to a diverse group of compounds used as protection against herbivores by plants in the family Brassicaceae, while in vertebrates, ingested glucosinolates exert health-...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1537-y
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:Lymphatic filariasis and onchocerciasis are debilitating diseases caused by filarial nematodes. Disease pathogenesis is induced by inflammatory responses following the death of the parasite. Wolbachia endosymbionts of filariae are potent inducers of innate and adaptive inflammation and bacterial lipoproteins...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-99
更新日期:2010-10-14 00:00:00
abstract:BACKGROUND:The emergence and massive spread of bluetongue in Western Europe during 2006-2008 had disastrous consequences for sheep and cattle production and confirmed the ability of Palaearctic Culicoides (Diptera: Ceratopogonidae) to transmit the virus. Some aspects of Culicoides ecology, especially host-seeking and f...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-119
更新日期:2011-06-27 00:00:00
abstract:BACKGROUND:Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-66
更新日期:2013-03-15 00:00:00
abstract:BACKGROUND:Thelazia callipaeda is a zoonotic nematode that affects the eyes of domestic and wild animals, including dogs, cats and red foxes. This parasitic eye worm is transmitted by Phortica variegata, which is a zoophilic fruit fly spread in Europe. Two wild European rabbits (Oryctolagus cuniculus) found dead in nor...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1526-1
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:Infections by protozoans of the genus Giardia are a common cause of diarrhea in dogs. Canine giardiosis constitutes a disease with a zoonotic potential; however, it is often underestimated due to its challenging diagnosis. The objective of the study was to assess the diagnostic performance of an immunochroma...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04422-6
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:The tropical bed bug, Cimex hemipterus, is a serious indoor public health pest in tropical regions causing intense physical discomfort and mental distress to humans. At present, the application of insecticides is the major control strategy. The present study was designed to evaluate the development of resist...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3565-x
更新日期:2019-06-21 00:00:00
abstract:BACKGROUND:Dirofilaria repens is the causative agent of subcutaneous dirofilariosis of dogs, other animals and humans. This nematode is transmitted by mosquitoes of Aedes, Anopheles and Culex genera. In dogs, the parasite may cause subclinical infection or cutaneous signs. Recently, D. repens has emerged and spread in ...
journal_title:Parasites & vectors
pub_type: 杂志文章,随机对照试验
doi:10.1186/1756-3305-7-347
更新日期:2014-07-29 00:00:00
abstract:BACKGROUND:Zoonotic onchocerciasis is a vector-borne disease, which involves many animal species, including large ungulates, boars, dogs, and sporadically, humans. So far, 39 cases of zoonotic onchocerciasis have been reported worldwide, 30 of which have been found in the last 20 years. Onchocerca nematodes are transmi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3925-6
更新日期:2020-02-12 00:00:00
abstract:BACKGROUND:With the spread of pyrethroid resistance in mosquitoes, the combination of an insecticide (carbamate or organophosphate) with a repellent (DEET) is considered as a promising alternative strategy for the treatment of mosquito nets and other relevant materials. The efficacy of these mixtures comes from the fac...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-2-17
更新日期:2009-04-16 00:00:00
abstract::Acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. Results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-016-1719-7
更新日期:2016-08-08 00:00:00
abstract:BACKGROUND:Studies of avian haemosporidians allow understanding how these parasites affect wild bird populations, and if their presence is related to factors such as habitat loss, degradation and fragmentation, and climate change. Considering the importance of the highland Plateau of Mexico as part of the North America...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1569-3
更新日期:2016-05-27 00:00:00
abstract:BACKGROUND:Ecto-Nucleoside Triphosphate Diphosphohydrolases (Ecto-NTPDases) are enzymes that hydrolyze tri- and/or di-phosphate nucleotides. Evidences point to their participation in Trypanosoma cruzi virulence and infectivity. In this work, we evaluate TcNTPDase-1 gene expression in comparison with ecto-NTPDase activi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0463-0
更新日期:2014-10-05 00:00:00
abstract:BACKGROUND:Surveillance of outdoor resting malaria vector populations is crucial to monitor possible changes in vector resting and feeding behaviour following the widespread use of indoor-based vector control interventions. However, it is seldom included in the routine vector surveillance system in Africa due to lack o...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3535-3
更新日期:2019-05-31 00:00:00
abstract:BACKGROUND:Lipoproteins are the major agonists of Wolbachia-dependent inflammatory pathogenesis in filariasis and a validated target for drug discovery. Here we characterise the abundance, localisation and serology of the Wolbachia lipoproteins: Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion S...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0462-1
更新日期:2014-10-06 00:00:00
abstract:BACKGROUND:Babesia spp. are apicomplexan parasites which infect a wide range of mammalian hosts. Historically, most Babesia species were described based on the assumed host specificity and morphological features of the intraerythrocytic stages. New DNA-based approaches challenge the traditional species concept and host...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04051-z
更新日期:2020-04-03 00:00:00
abstract:BACKGROUND:The strongylid nematode Haemonchus contortus is a parasite of major concern for modern livestock husbandry because hostile environmental conditions may induce diapause in the early fourth-stage larvae. METHODS:A new gene Hc-daf-22 was identified which is the homologue of Ce-daf-22 and human SCPx. Genome wal...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1704-1
更新日期:2016-07-29 00:00:00
abstract:BACKGROUND:A new isolate of Aura virus serendipitously discovered as a cell culture contaminant is reported in this manuscript. Aura virus belongs to the family Togaviridae and is classified in the genus Alphavirus. There are only two reports of Aura virus isolation from mosquitoes in the scientific literature, and the...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2907-4
更新日期:2018-05-29 00:00:00
abstract:BACKGROUND:In Brazil, the autochthonous transmission of extra-Amazonian malaria occurs mainly in areas of the southeastern coastal Atlantic Forest, where Anopheles cruzii is the primary vector. In these locations, the population density of the mosquito varies with altitude (5-263 m above sea level), prompting us to hyp...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0581-8
更新日期:2014-12-16 00:00:00
abstract:BACKGROUND:Giardia duodenalis is a flagellated parasite that causes diarrhea in humans and other animals. Although G. duodenalis is found in companion animals worldwide, information regarding the prevalence and genetic characteristics of G. duodenalis in pet chipmunks in China is limited. The present study therefore ai...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2790-z
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:Understanding local Anopheles species compositions and bionomic traits are vital for an effective malaria vector intervention strategy. Though eight malaria vectors, including species complexes, have been documented across the island of Sulawesi, Indonesia, a comprehensive survey linking morphological and mo...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04252-6
更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:Phagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval st...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1961-z
更新日期:2017-01-31 00:00:00
abstract:BACKGROUND:Both falciparum and vivax malaria were historically prevalent in China with high incidence. With the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000. Despite this, the re-emergence or outbreak of malaria was unavoidable...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-136
更新日期:2012-07-09 00:00:00
abstract:BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0608-1
更新日期:2015-01-06 00:00:00
abstract:BACKGROUND:More effective mosquito control strategies are urgently required due to the increasing prevalence of insecticide resistance. The sterile insect technique (SIT) and the release of insects carrying a dominant lethal allele (RIDL) are two proposed methods for environmentally-friendly, species-targeted populatio...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1331-x
更新日期:2016-01-28 00:00:00
abstract:BACKGROUND:Taenia multiceps is a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3352-8
更新日期:2019-03-12 00:00:00