Molecular analysis reveals a high diversity of Anopheles species in Karama, West Sulawesi, Indonesia.

Abstract:

BACKGROUND:Understanding local Anopheles species compositions and bionomic traits are vital for an effective malaria vector intervention strategy. Though eight malaria vectors, including species complexes, have been documented across the island of Sulawesi, Indonesia, a comprehensive survey linking morphological and molecular species identification has not been conducted in this global hotspot of biodiversity. RESULTS:Eighteen distinct species of Anopheles were molecularly identified in a 1 km2 area in Karama village, West Mamuju Province, Sulawesi. Known species included An. aconitus, An. karwari, An. peditaeniatus, An. vagus, An. barbirostris, An. tessellatus, An. nigerrimus, An. crawfordi, An. maculatus, An. flavirostris and An. kochi. Of the 18 distinct sequence groups identified through both ribosomal DNA internal transcribed spacer region 2, and mitochondrial DNA cytochrome c oxidase subunit 1 loci, 8 could not be identified to species through comparison to published sequences. The comparison of morphological and molecular identities determined that interpretations of local species compositions for primary and expected species in Karama (An. barbirostris and An. vagus) had the highest rate of accuracy (92.1% and 87.6%, respectively) when compared to molecular analysis. However, the remaining distinct sequences molecularly identified to species were identified correctly by morphological methods less frequently, from 0 to 83%. CONCLUSIONS:Karama, Indonesia has a high diversity of Anopheles spp. The unexpected high number of Anopheles species in a small area points to possible complex transmission dynamics and limitations with vector control based on possible varying behaviors and interactions with both humans and interventions. Morphological identification of Anopheles spp. in this study was more accurate for primary and expected species than secondary or unexpected species. Finally, the inability to identify seven sequence groups to species with consensus sequences implies that future studies employing sequencing are required to clarify species compositions in the Nigerrimus Subgroup, among others, as well as their distribution and vector status. Use of molecular methods in conjunction with morphological investigations for analysis of species composition, population dynamics and bionomic characteristics is directly implicated in understanding drivers of malaria transmission, intervention effectiveness, and the pursuit of malaria elimination.

journal_name

Parasit Vectors

journal_title

Parasites & vectors

authors

Davidson JR,Wahid I,Sudirman R,Small ST,Hendershot AL,Baskin RN,Burton TA,Makuru V,Xiao H,Yu X,Troth EV,Olivieri D,Lizarraga S,Hasan H,Arfah A,Yusuf M,Nur N,Syafruddin D,Asih P,Lobo NF

doi

10.1186/s13071-020-04252-6

subject

Has Abstract

pub_date

2020-07-29 00:00:00

pages

379

issue

1

issn

1756-3305

pii

10.1186/s13071-020-04252-6

journal_volume

13

pub_type

杂志文章
  • Parasite load and genotype are associated with clinical outcome of piroplasm-infected equines in Israel.

    abstract:BACKGROUND:Equine piroplasmosis is a highly endemic protozoan disease of horses worldwide, caused by Theileria equi and Babesia caballi. While most horses in endemic areas are subclinically infected, the mechanisms leading to clinical outcome are vastly unknown. Moreover, since clinical signs of disease are not specifi...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-020-04133-y

    authors: Tirosh-Levy S,Steinman A,Levy H,Katz Y,Shtilman M,Gottlieb Y

    更新日期:2020-05-20 00:00:00

  • Prevalence and diversity of Rickettsia species in ectoparasites collected from small rodents in Lithuania.

    abstract:BACKGROUND:Rickettsiae are emerging pathogens causing public health problems in many countries around the world. Rickettsia spp. are found in association with a wide range of arthropods which feed on different species of animals. However, the distribution and natural cycle of Rickettsia species and their association wi...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2947-9

    authors: Radzijevskaja J,Kaminskienė E,Lipatova I,Mardosaitė-Busaitienė D,Balčiauskas L,Stanko M,Paulauskas A

    更新日期:2018-06-28 00:00:00

  • Nitric oxide and cytokine production by glial cells exposed in vitro to neuropathogenic schistosome Trichobilharzia regenti.

    abstract:BACKGROUND:Helminth neuroinfections represent a serious health problem, but host immune mechanisms in the nervous tissue often remain undiscovered. This study aims at in vitro characterization of the response of murine astrocytes and microglia exposed to Trichobilharzia regenti which is a neuropathogenic schistosome mi...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1869-7

    authors: Macháček T,Panská L,Dvořáková H,Horák P

    更新日期:2016-11-14 00:00:00

  • The influence of larval competition on Brazilian Wolbachia-infected Aedes aegypti mosquitoes.

    abstract:BACKGROUND:With field releases starting in Brazil, particular interest must be given to understanding how the endosymbiotic bacterium Wolbachia pipientis affects Aedes aegypti mosquitoes with a Brazilian genetic background. Currently, there is limited information on how the bacterium affects phenotypic traits such as l...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1559-5

    authors: Dutra HL,Lopes da Silva V,da Rocha Fernandes M,Logullo C,Maciel-de-Freitas R,Moreira LA

    更新日期:2016-05-16 00:00:00

  • Controlled efficacy trial confirming toltrazuril resistance in a field isolate of ovine Eimeria spp.

    abstract:BACKGROUND:Coccidiosis due to Eimeria spp. infections in lambs causes increased mortality and substantial production losses, and anticoccidials are important for control of the infection. Anticoccidial resistance has been reported in poultry and swine, and we recently described reduced toltrazuril efficacy in ovine Eim...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2976-4

    authors: Odden A,Enemark HL,Ruiz A,Robertson LJ,Ersdal C,Nes SK,Tømmerberg V,Stuen S

    更新日期:2018-07-05 00:00:00

  • Development of environmental tools for anopheline larval control.

    abstract:BACKGROUND:Malaria mosquitoes spend a considerable part of their life in the aquatic stage, rendering them vulnerable to interventions directed to aquatic habitats. Recent successes of mosquito larval control have been reported using environmental and biological tools. Here, we report the effects of shading by plants a...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-4-130

    authors: Imbahale SS,Mweresa CK,Takken W,Mukabana WR

    更新日期:2011-07-06 00:00:00

  • The abundance and host-seeking behavior of culicine species (Diptera: Culicidae) and Anopheles sinensis in Yongcheng city, People's Republic of China.

    abstract:BACKGROUND:The knowledge of mosquito species diversity and the level of anthropophily exhibited by each species in a region are of great importance to the integrated vector control. Culicine species are the primary vectors of Japanese encephalitis (JE) virus and filariasis in China. Anopheles sinensis plays a major rol...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-4-221

    authors: Liu XB,Liu QY,Guo YH,Jiang JY,Ren DS,Zhou GC,Zheng CJ,Zhang Y,Liu JL,Li ZF,Chen Y,Li HS,Morton LC,Li HZ,Li Q,Gu WD

    更新日期:2011-11-24 00:00:00

  • Structural and functional characterization of a novel gene, Hc-daf-22, from the strongylid nematode Haemonchus contortus.

    abstract:BACKGROUND:The strongylid nematode Haemonchus contortus is a parasite of major concern for modern livestock husbandry because hostile environmental conditions may induce diapause in the early fourth-stage larvae. METHODS:A new gene Hc-daf-22 was identified which is the homologue of Ce-daf-22 and human SCPx. Genome wal...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1704-1

    authors: Guo X,Zhang H,Zheng X,Zhou Q,Yang Y,Chen X,Du A

    更新日期:2016-07-29 00:00:00

  • Therapeutic efficacy of Artemether/Lumefantrine (Coartem(R)) against Plasmodium falciparum in Kersa, South West Ethiopia.

    abstract:BACKGROUND:Artemether/Lumefantrine (Coartem(R)) has been used as a first-line treatment for uncomplicated Plasmodium falciparum infection since 2004 in Ethiopia. In the present study the therapeutic efficacy of artemether/lumefantrine for the treatment of uncomplicated P. falciparum infection in Kersa, Jima zone, South...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-3-1

    authors: Assefa A,Kassa M,Tadese G,Mohamed H,Animut A,Mengesha T

    更新日期:2010-01-05 00:00:00

  • Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.

    abstract:BACKGROUND:Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, causing quite pronounced...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-019-3429-4

    authors: Kumarasingha R,Young ND,Yeo TC,Lim DSL,Tu CL,Palombo EA,Shaw JM,Gasser RB,Boag PR

    更新日期:2019-04-25 00:00:00

  • Histological and parasitological distinctive findings in clinically-lesioned and normal-looking skin of dogs with different clinical stages of leishmaniosis.

    abstract:BACKGROUND:Normal-looking skin of dogs with leishmaniosis frequently shows microscopic lesions along with the presence of Leishmania amastigotes. However, histological lesions with or without detection of amastigotes might not occur in less severe clinical cases. In addition, comparative studies between paired clinical...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2051-6

    authors: Ordeix L,Dalmau A,Osso M,Llull J,Montserrat-Sangrà S,Solano-Gallego L

    更新日期:2017-03-13 00:00:00

  • Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection.

    abstract:BACKGROUND:Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic areas. Recombinase Polymerase Amplific...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-015-1055-3

    authors: Rosser A,Rollinson D,Forrest M,Webster BL

    更新日期:2015-09-04 00:00:00

  • The oxidative status and inflammatory level of the peripheral blood of rabbits infested with Psoroptes cuniculi.

    abstract:BACKGROUND:Psoroptes cuniculi can parasitise the ear canal of the rabbit, and cause the afflicted animals to cease feeding and become severely debilitated, sometimes resulting in death. In this study, we examined the oxidative status and inflammatory level of the peripheral blood of rabbits infested with Psoroptes cuni...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-7-124

    authors: Shang X,Wang D,Miao X,Wang X,Li J,Yang Z,Pan H

    更新日期:2014-03-25 00:00:00

  • Are fish immune systems really affected by parasites? An immunoecological study of common carp (Cyprinus carpio).

    abstract:BACKGROUND:The basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. According to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. Concerning fish, both phy...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-4-120

    authors: Rohlenová K,Morand S,Hyršl P,Tolarová S,Flajšhans M,Simková A

    更新日期:2011-06-27 00:00:00

  • Ultrastructure and localization of Neorickettsia in adult digenean trematodes provides novel insights into helminth-endobacteria interaction.

    abstract:BACKGROUND:Neorickettsia are a group of intracellular α proteobacteria transmitted by digeneans (Platyhelminthes, Trematoda). These endobacteria can also infect vertebrate hosts of the helminths and cause serious diseases in animals and humans. Neorickettsia have been isolated from infected animals and maintained in ce...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2123-7

    authors: Fischer K,Tkach VV,Curtis KC,Fischer PU

    更新日期:2017-04-13 00:00:00

  • Spatial and seasonal variation in the prevalence of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in questing Ixodes ricinus ticks in Norway.

    abstract:BACKGROUND:Understanding the variation in prevalence of Borrelia burgdorferi sensu lato (Lyme Borreliosis Spirochaetes, LBS) and Anaplasma phagocytophilum (causing tick-borne fever in ruminants and human granulocytic ehrlichiosis) in ticks is vital from both a human and an animal disease perspective to target the most ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-6-187

    authors: Mysterud A,Easterday WR,Qviller L,Viljugrein H,Ytrehus B

    更新日期:2013-06-20 00:00:00

  • Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle.

    abstract:BACKGROUND:In East Africa, animal trypanosomiasis is caused by many tsetse transmitted protozoan parasites including Trypanosoma vivax, T. congolense and subspecies of T. brucei s.l. (T. b. brucei and zoonotic human infective T. b. rhodesiense) that may co-circulate in domestic and wild animals. Accurate species-specif...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-3-82

    authors: Cox AP,Tosas O,Tilley A,Picozzi K,Coleman P,Hide G,Welburn SC

    更新日期:2010-09-06 00:00:00

  • Parasitism of Aedes albopictus by Ascogregarina taiwanensis lowers its competitive ability against Aedes triseriatus.

    abstract:BACKGROUND:Mosquitoes are vectors for diseases such as dengue, malaria and La Crosse virus that significantly impact the human population. When multiple mosquito species are present, the competition between species may alter population dynamics as well as disease spread. Two mosquito species, Aedes albopictus and Aedes...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-021-04581-0

    authors: Stump E,Childs LM,Walker M

    更新日期:2021-01-25 00:00:00

  • Establishment and interspecific associations in two species of Ichthyocotylurus (Trematoda) parasites in perch (Perca fluviatilis).

    abstract:BACKGROUND:Co-infections of multiple parasite species in hosts may lead to interspecific associations and subsequently shape the structure of a parasite community. However, few studies have focused on these associations in highly abundant parasite species or, in particular, investigated how the associations develop wit...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-4-85

    authors: Faltýnková A,Karvonen A,Valtonen ET

    更新日期:2011-05-20 00:00:00

  • Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system.

    abstract:BACKGROUND:Toxoplasma gondii, an obligate intracellular protozoan parasite, possesses the remarkable ability to co-opt host cell machinery in order to maintain its intracellular survival. This parasite can modulate signaling pathways of its host through the secretion of polymorphic effector proteins localized in the rh...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1943-1

    authors: Liu Q,Li FC,Elsheikha HM,Sun MM,Zhu XQ

    更新日期:2017-01-03 00:00:00

  • Retrospective investigation of Echinococcus canadensis emergence in translocated elk (Cervus canadensis) in Tennessee, USA, and examination of canid definitive hosts.

    abstract:BACKGROUND:Few reports of Echinococcus spp. have been described in the USA; however, the geographical distribution of Echinococcus spp. in wild hosts is increasing consequent to human activities. In the early 2000's, 253 elk (Cervus canadensis) originating from Alberta, Canada were released into the Great Smoky Mountai...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-020-04198-9

    authors: Dell B,Newman SJ,Purple K,Miller B,Ramsay E,Donnell R,Gerhold RW

    更新日期:2020-06-30 00:00:00

  • Blastocystis sp. in splenic cysts: causative agent or accidental association? A unique case report.

    abstract:BACKGROUND:Blastocystis sp. is one of the most prevalent parasites found in human stool and has been recently considered an opportunistic emerging pathogen in immunocompromised individuals. However, cases of invasive intestinal infections and skin rashes have been attributed to infection by Blastocystis sp in immunocom...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-7-207

    authors: Santos HL,Sodré FC,de Macedo HW

    更新日期:2014-04-29 00:00:00

  • Survey of Anaplasma phagocytophilum and Anaplasma sp. 'Omatjenne' infection in cattle in Africa with special reference to Ethiopia.

    abstract:BACKGROUND:As evidence of the infection of domestic animals by Anaplasma phagocytophilum and Anaplasma sp. 'Omatjenne' is presently becoming available, understanding the epidemiological and ecological significance of infection is important to quantify the clinical and socio-economic impact of the diseases they cause. ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2633-y

    authors: Teshale S,Geysen D,Ameni G,Dorny P,Berkvens D

    更新日期:2018-03-09 00:00:00

  • Variation in species composition and infection rates of Anopheles mosquitoes at different altitudinal transects, and the risk of malaria in the highland of Dirashe Woreda, south Ethiopia.

    abstract:BACKGROUND:The transmission of malaria is heterogeneous, and varies due to altitude. The information on whether the transmission of malaria is indigenous or imported to highland areas is scarce. Therefore, this study aimed to assess the species composition and infection rates of Anopheles at different altitudinal trans...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2288-0

    authors: Daygena TY,Massebo F,Lindtjørn B

    更新日期:2017-07-19 00:00:00

  • Countrywide serological evaluation of canine prevalence for Anaplasma spp., Borrelia burgdorferi (sensu lato), Dirofilaria immitis and Ehrlichia canis in Mexico.

    abstract:BACKGROUND:Canine vector-borne diseases (CVBD) have become a major concern for canine and human public health. The aim of the study described here is to add epidemiological data regarding four pathogens responsible for CVBD, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducte...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1686-z

    authors: Movilla R,García C,Siebert S,Roura X

    更新日期:2016-07-29 00:00:00

  • Modelling local areas of exposure to Schistosoma japonicum in a limited survey data environment.

    abstract:BACKGROUND:Spatial modelling studies of schistosomiasis (SCH) are now commonplace. Covariate values are commonly extracted at survey locations, where infection does not always take place, resulting in an unknown positional exposure mismatch. The present research aims to: (i) describe the nature of the positional exposu...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-3039-6

    authors: Araujo Navas AL,Soares Magalhães RJ,Osei F,Fornillos RJC,Leonardo LR,Stein A

    更新日期:2018-08-13 00:00:00

  • Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection.

    abstract:BACKGROUND:Insect haemocytes mediate cellular immune responses (e.g., phagocytosis) and contribute to the synthesis of humoral immune factors. In previous work, a genome-wide molecular characterization of Anopheles gambiae circulating haemocytes was followed by functional gene characterization using cell-based RNAi scr...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1359-y

    authors: Lombardo F,Christophides GK

    更新日期:2016-02-09 00:00:00

  • Comparative pharmacokinetics of fluralaner in dogs and cats following single topical or intravenous administration.

    abstract:BACKGROUND:Bravecto™ Chewable Tablets for Dogs, containing fluralaner as active ingredient, is an innovative treatment for flea and tick infestations that provides safe, rapid and long acting efficacy after a single oral administration in dogs. Topically applied fluralaner provides similar safe, rapid and long acting e...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1564-8

    authors: Kilp S,Ramirez D,Allan MJ,Roepke RK

    更新日期:2016-05-31 00:00:00

  • Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds.

    abstract:BACKGROUND:Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the serop...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1753-5

    authors: Djokic V,Fablet C,Blaga R,Rose N,Perret C,Djurkovic-Djakovic O,Boireau P,Durand B

    更新日期:2016-08-24 00:00:00

  • Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China.

    abstract:BACKGROUND:The protistan pathogens Cryptosporidium and Giardia can cause significant intestinal diseases in animals and humans. Cattle, particularly calves, carrying these protists can be significant reservoirs for human infections and disease. However, little is known about the genetic make-up of Cryptosporidium and G...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2463-3

    authors: Fan Y,Wang T,Koehler AV,Hu M,Gasser RB

    更新日期:2017-10-25 00:00:00