Abstract:
BACKGROUND:The disease scabies, caused by the ectoparasitic mite, Sarcoptes scabiei, causes significant morbidity in humans and other mammals worldwide. However, there is limited data available regarding the molecular basis of host specificity and host-parasite interactions. Therefore, we sought to produce a draft genome for S. scabiei and use this to identify molecular markers that will be useful for phylogenetic population studies and to identify candidate protein-coding genes that are critical to the unique biology of the parasite. METHODS:S. scabiei var. canis DNA was isolated from living mites and sequenced to ultra-deep coverage using paired-end technology. Sequence reads were assembled into gapped contigs using de Bruijn graph based algorithms. The assembled genome was examined for repetitive elements and gene annotation was performed using ab initio, and homology-based methods. RESULTS:The draft genome assembly was about 56.2 Mb and included a mitochondrial genome contig. The predicted proteome contained 10,644 proteins, ~67 % of which appear to have clear orthologs in other species. The genome also contained more than 140,000 simple sequence repeat loci that may be useful for population-level studies. The mitochondrial genome contained 13 protein coding loci and 20 transfer RNAs. Hundreds of candidate salivary gland protein genes were identified by comparing the scabies mite predicted proteome with sialoproteins and transcripts identified in ticks and other hematophagous arthropods. These include serpins, ferritins, reprolysins, apyrases and new members of the macrophage migration inhibitory factor (MIF) gene family. Numerous other genes coding for salivary proteins, metabolic enzymes, structural proteins, proteins that are potentially immune modulating, and vaccine candidates were identified. The genes encoding cysteine and serine protease paralogs as well as mu-type glutathione S-transferases are represented by gene clusters. S. scabiei possessed homologs for most of the 33 dust mite allergens. CONCLUSION:The draft genome is useful for advancing our understanding of the host-parasite interaction, the biology of the mite and its phylogenetic relationship to other Acari. The identification of antigen-producing genes, candidate immune modulating proteins and pathways, and genes responsible for acaricide resistance offers opportunities for developing new methods for diagnosing, treating and preventing this disease.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Rider SD Jr,Morgan MS,Arlian LGdoi
10.1186/s13071-015-1198-2subject
Has Abstractpub_date
2015-11-10 00:00:00pages
585issn
1756-3305pii
10.1186/s13071-015-1198-2journal_volume
8pub_type
杂志文章abstract:BACKGROUND:Cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. The purpose of this study was to identify a cathepsin B of Clonorchis sinensis (CsCB) and to investiga...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-149
更新日期:2011-07-27 00:00:00
abstract:BACKGROUND:Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the serop...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1753-5
更新日期:2016-08-24 00:00:00
abstract:BACKGROUND:A blinded, randomized, negative controlled laboratory study was conducted to evaluate the efficacy of lotilaner (CredelioTM, Elanco) when administered orally to dogs, against experimentally induced adult flea infestations and flea egg production. METHODS:Twenty dogs were selected for the study and allocated...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3873-1
更新日期:2020-01-14 00:00:00
abstract:BACKGROUND:The host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. Although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1659-2
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Lymphatic filariasis and onchocerciasis are debilitating diseases caused by filarial nematodes. Disease pathogenesis is induced by inflammatory responses following the death of the parasite. Wolbachia endosymbionts of filariae are potent inducers of innate and adaptive inflammation and bacterial lipoproteins...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-99
更新日期:2010-10-14 00:00:00
abstract:BACKGROUND:Liver fibrosis is an excessive wound-healing reaction that requires the participation of inflammatory cells and hepatic stellate cells (HSCs). The pathogenesis of liver fibrosis caused by viruses and alcohol has been well characterized, but the molecular mechanisms underlying liver fibrosis induced by the li...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2228-z
更新日期:2017-06-17 00:00:00
abstract:BACKGROUND:Cytauxzoonosis is an emerging tick-borne disease of domestic and wild felids. Cytauxzoon felis induces severe and often fatal disease in domestic cats. In Europe, clinical and subclinical infections caused by Cytauxzoon sp. are described. We report the first cases of Cytauxzoon sp. infection in domestic cats...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2728-5
更新日期:2018-05-10 00:00:00
abstract:BACKGROUND:Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1687-y
更新日期:2016-07-25 00:00:00
abstract:BACKGROUND:Intestinal parasite infections (IPIs) are among the most significant causes of illness and disease of socially and economically disadvantaged populations in developing countries, including rural areas of the People's Republic of China. With the spread of the human immunodeficiency virus (HIV) among rural Chi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-36
更新日期:2012-02-13 00:00:00
abstract:BACKGROUND:The parasitic mite Varroa destructor is considered the major pest of the European honey bee (Apis mellifera) and responsible for declines in honey bee populations worldwide. Exploiting the full potential of gene sequences becoming available for V. destructor requires adaptation of modern molecular biology ap...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-73
更新日期:2010-08-16 00:00:00
abstract:BACKGROUND:Multiplicity and genetic diversity of Plasmodium falciparum infection might play a role in determining the clinical outcome of malaria infection and could be a fair reflection of the disease transmission rate. This study investigated the genetic diversity of P. falciparum and multiplicity of infection in rel...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1641-z
更新日期:2016-06-27 00:00:00
abstract:BACKGROUND:The emergence and spread of insecticide resistance in the major African malaria vectors Anopheles gambiae (s.s.) and An. arabiensis may compromise the current vector control interventions and threatens the global malaria control and elimination efforts. METHODS:Insecticide resistance was monitored in severa...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2342-y
更新日期:2017-09-02 00:00:00
abstract:BACKGROUND:Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. METHODS:A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Eryth...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0872-8
更新日期:2015-05-15 00:00:00
abstract:BACKGROUND:Normal-looking skin of dogs with leishmaniosis frequently shows microscopic lesions along with the presence of Leishmania amastigotes. However, histological lesions with or without detection of amastigotes might not occur in less severe clinical cases. In addition, comparative studies between paired clinical...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2051-6
更新日期:2017-03-13 00:00:00
abstract:BACKGROUND:The East Route Project (ERP) of the South-to-North Water Diversion Project (SNWDP) stretches across schistosomiasis endemic and non-endemic areas in China, which may lead to the dispersal of Oncomelania hupensis, the intermediate host of Schistosoma japonicum, from permissive areas along the Yangtze River Ba...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-4004-8
更新日期:2020-03-19 00:00:00
abstract:BACKGROUND:In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, B...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1963-x
更新日期:2017-01-24 00:00:00
abstract:BACKGROUND:Rhipicephalus sanguineus is a ubiquitous tick responsible for transmitting Ehrlichia canis and most likely Anaplasma platys to dogs, as either single or co-infections. The objective of this study was to assess the effects of either simultaneous or sequential experimental infections with E. canis and A. platy...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-33
更新日期:2010-04-08 00:00:00
abstract:BACKGROUND:Canine vector-borne diseases (CVBD) have become a major concern for canine and human public health. The aim of the study described here is to add epidemiological data regarding four pathogens responsible for CVBD, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducte...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1686-z
更新日期:2016-07-29 00:00:00
abstract:BACKGROUND:Understanding the dispersal dynamics of invasive mosquito species is fundamental to improve vector surveillance and to target control efforts. Aedes albopictus has been deemed a poor flyer as its range of active dispersal is generally assumed to be limited to a few hundred metres, while laboratory studies su...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3837-5
更新日期:2019-12-12 00:00:00
abstract:BACKGROUND:Estimating the abundance of Culicoides using light traps is influenced by a large variation in abundance in time and place. This study investigates the optimal trapping strategy to estimate the abundance or presence/absence of Culicoides on a field with grazing animals. We used 45 light traps to sample speci...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-151
更新日期:2013-05-24 00:00:00
abstract:BACKGROUND:To date there has been no evidence of mosquito-borne virus transmission of public health concern in the UK, despite the occurrence of more than 30 species of mosquito, including putative vectors of arboviruses. The saltmarsh mosquito Ochlerotatus detritus [syn. Aedes (Ochlerotatus) detritus] is locally commo...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1739-3
更新日期:2016-08-15 00:00:00
abstract:BACKGROUND:Knowledge of insecticide resistance status in the main malaria vectors is an essential component of effective malaria vector control. This study presents the first evaluation of the status of insecticide resistance in Anopheles gambiae populations from Bangui, the Central African Republic. METHODS:Anopheles...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1965-8
更新日期:2017-01-13 00:00:00
abstract:BACKGROUND:African trypanosomes are extracellular protozoan parasites that are transmitted between mammalian hosts by the bite of an infected tsetse fly. Human African Trypanosomiasis (HAT) or sleeping sickness is caused by Trypanosoma brucei rhodesiense or T. brucei gambiense, while African Animal Trypanosomiasis (AAT...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-217
更新日期:2012-10-01 00:00:00
abstract::The need to discover new treatments for human schistosomiasis has been an important driver for molecular research on schistosomes, a major breakthrough being the publication of the Schistosoma mansoni and Schistosoma japonicum genomes in 2009. This 'Primer' considers recent advances in the understanding of schistosome...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-203
更新日期:2011-10-20 00:00:00
abstract:BACKGROUND:Schistosomiasis remains prevalent in Africa, Asia and South America with an estimated burden of 1.9 million disability-adjusted life years in 2016. Targeting snails as a key to success for schistosomiasis control has been widely approved, but the long-term quantitative effects of interventions on snail contr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04273-1
更新日期:2020-08-05 00:00:00
abstract:BACKGROUND:As part of efforts to more fully understand the potential risks posed by West Nile virus (WNV) and Usutu virus (USUV) in the UK, and following on from previous reports of a potential bridge vector Culex modestus for these viruses, at wetland sites in North Kent, mosquito surveillance was undertaken more wide...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0705-9
更新日期:2015-02-10 00:00:00
abstract:BACKGROUND:Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1344-5
更新日期:2016-02-02 00:00:00
abstract:BACKGROUND:Insect haemocytes mediate cellular immune responses (e.g., phagocytosis) and contribute to the synthesis of humoral immune factors. In previous work, a genome-wide molecular characterization of Anopheles gambiae circulating haemocytes was followed by functional gene characterization using cell-based RNAi scr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1359-y
更新日期:2016-02-09 00:00:00
abstract:BACKGROUND:Cutaneous Leishmaniasis (CL) is endemic in Sudan. It is caused by Leishmania major parasites and transmitted by Phlebotomus papatasi sandflies. Recently, uncommon clinical manifestations of CL have been reported. Moreover, L. donovani parasites that cause Visceral Leishmaniasis (VL) have been isolated from C...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-249
更新日期:2012-11-12 00:00:00
abstract:BACKGROUND:The population dynamics of vector-borne pathogens inside the arthropod vector can have important consequences for vector-to-host transmission. Tick-borne spirochete bacteria of the Borrelia burgdorferi (sensu lato) species complex cause Lyme borreliosis in humans and spend long periods of time (>12 months) i...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2187-4
更新日期:2017-05-25 00:00:00