Abstract:
BACKGROUND:Liver fibrosis is an excessive wound-healing reaction that requires the participation of inflammatory cells and hepatic stellate cells (HSCs). The pathogenesis of liver fibrosis caused by viruses and alcohol has been well characterized, but the molecular mechanisms underlying liver fibrosis induced by the liver fluke Clonorchis sinensis are poorly understood. Lysophospholipase A (LysoPLA), which deacylates lysophospholipids, plays a critical role in mediating the virulence and pathogenesis of parasites and fungi; however, the roles of C. sinensis lysophospholipase A (CsLysoPLA) in C. sinensis-induced liver fibrosis remain unknown. METHODS:A mouse macrophage cell line (RAW264.7) was cultured and treated with CsLysoPLA. IL-25 and members of its associated signaling pathway were detected by performing quantitative real-time PCR, Western blotting and immunofluorescent staining. A human hepatic stellate cell line (LX-2) was cultured and exposed to IL-25. LX-2 cell activation markers were examined via quantitative real-time PCR, Western blotting and immunofluorescent staining. Migration was analyzed in transwell plates. RESULTS:Treating RAW264.7 cells with CsLysoPLA significantly induced IL-25 expression. Elevated PKA, B-Raf, and ERK1/2 mRNA levels and phosphorylated B-Raf and ERK1/2 were detected in CsLysoPLA-stimulated RAW264.7 cells. The PKA inhibitor H-89 weakened B-Raf and ERK1/2 phosphorylation whereas the AKT activator SC79 attenuated ERK1/2 phosphorylation in RAW264.7 cells. Both H-89 and SC79 inhibited CsLysoPLA-induced IL-25 upregulation. In addition, stimulation of LX-2 cells with IL-25 upregulated the expression of mesenchymal cell markers, including α-smooth muscle actin (α-SMA) and collagen type I (Collagen-I), and promoted cell migration. CONCLUSIONS:CsLysoPLA activates HSCs by upregulating IL-25 in macrophages through the PKA-dependent B-Raf/ERK1/2 pathway and potentially promotes hepatic fibrosis during C. sinensis infection.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Zhou L,Shi M,Zhao L,Lin Z,Tang Z,Sun H,Chen T,Lv Z,Xu J,Huang Y,Yu Xdoi
10.1186/s13071-017-2228-zsubject
Has Abstractpub_date
2017-06-17 00:00:00pages
295issue
1issn
1756-3305pii
10.1186/s13071-017-2228-zjournal_volume
10pub_type
杂志文章abstract:BACKGROUND:The identification of Trypanosoma cruzi and blood-meal sources in synanthropic triatomines is important to assess the potential risk of Chagas disease transmission. We identified T. cruzi infection and blood-meal sources of triatomines caught in and around houses in the state of Bahia, northeastern Brazil, a...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3849-1
更新日期:2019-12-26 00:00:00
abstract:BACKGROUND:Bioclimatic variables play an integral part in the life-cycle of Ctenocephalides felis, the most common flea found on companion animals. It is essential that we understand the effects of climate on C. felis distribution as fleas are a major veterinary and public health concern. This study investigated the cu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3399-6
更新日期:2019-03-22 00:00:00
abstract:BACKGROUND:Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2148-y
更新日期:2017-04-24 00:00:00
abstract:BACKGROUND:Anopheles culicifacies s.l. is one of the primary vectors of malaria in India responsible for the highest number of malaria cases. This vector is resistant to DDT in most parts of the country with indication of emerging resistance to pyrethroids. Since knockdown resistance (kdr) is known to confer cross-resi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0946-7
更新日期:2015-06-18 00:00:00
abstract:BACKGROUND:Canine dirofilariasis due to Dirofilaria immitis is known to be endemic in continental Portugal. However, information about the transmitting mosquito species is still scarce, with only Culex theileri identified to date, albeit with L1-2, through dissection. This study was carried out to investigate the poten...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0760-2
更新日期:2015-03-03 00:00:00
abstract:BACKGROUND:Diarrhea caused by opportunistic intestinal protozoa is a common problem in HIV infection. We aimed to establish the prevalence of Cryptosporidium, misrosporidia, and Isospora in HIV-infected people using a systematic review and meta-analysis, which is central to developing public policy and clinical service...
journal_title:Parasites & vectors
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s13071-017-2558-x
更新日期:2018-01-09 00:00:00
abstract:BACKGROUND:Proteins from the ABC family (ATP-binding cassette) represent the largest known group of efflux pumps, responsible for transporting specific molecules across lipid membranes in both prokaryotic and eukaryotic organisms. In arthropods they have been shown to play a role in insecticide defense/resistance. The ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-349
更新日期:2014-07-29 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3453-4
更新日期:2019-05-02 00:00:00
abstract:BACKGROUND:Schistosomiasis japonica remains a major public health concern in China. There are many interventions implemented to control the transmission of the disease. The purpose of the present study was to investigate the effects of an integrated control strategy for schistosomiasis control. METHODS:An integrated c...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-243
更新日期:2011-12-30 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3199-4
更新日期:2018-12-17 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-217
更新日期:2012-10-01 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-105
更新日期:2014-03-19 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-318
更新日期:2013-11-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04265-1
更新日期:2020-08-12 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3829-5
更新日期:2019-12-26 00:00:00
abstract:BACKGROUND:Despite similarities in morphology, gene and protein profiles, Entamoeba histolytica and E. moshkovskii show profound differences in pathogenicity. Entamoeba histolytica infection might result in amoebic dysentery and liver abscess, while E. moshkovskii causes only mild diarrhea. Extensive studies focus on r...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3363-5
更新日期:2019-03-13 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-224
更新日期:2014-05-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2020-03-02 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Parasites & vectors
pub_type: 已发布勘误
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更新日期:2020-09-22 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2019-10-22 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-17
更新日期:2012-01-13 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-36
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2014-09-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-117
更新日期:2014-03-24 00:00:00
abstract:BACKGROUND:Birds harbour an astonishing diversity of haemosporidian parasites. Renewed interest in avian haemosporidians as a model system has placed a greater emphasis on the development of screening protocols to estimate parasite prevalence and diversity. Prevalence estimates are often based on the molecular or blood...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2066-z
更新日期:2017-03-06 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2016-11-02 00:00:00