Abstract:
BACKGROUND:Opisthorchiasis and Opisthorchis viverrini-associated bile duct cancer represent major public health threats in Thailand and Laos. The tegument of this food borne fluke plays pivotal roles in parasite metabolism, homeostasis and osmoregulation. Excretory/secretory products also pass from the fluke to the biliary environment, products that likely underlie pathogenesis of liver fluke infection. Aquaporins (AQPs), belong to the major intrinsic protein superfamily of integral plasma membrane channel proteins that selectively transport water across cell membranes. AQPs play key roles as water and ion transport channels through the tegument of helminth parasites. METHODS:Here, two forms of AQP mRNAs from the adult developmental stage of O. viverrini, termed O. viverrini aquaporin-1 and -2 (Ov-aqp-1 and -2) were investigated. Roles of Ov-aqp-1 and -2 in the movement of water across the tegument of this carcinogenic liver fluke were investigated using RNA interference. RESULTS:Ov-AQP-1 and Ov-AQP-2 contain unique characteristic asparagine-proline-alanine (NPA) motifs of AQP transmembrane proteins. Phylogenetic analysis indicated that Ov-AQPs belong to an expanding group of aquaglyceroporin-like water channel proteins characterized from helminth and protozoan parasites, which is pivotal to the specialized requirements of water and solute control during parasitism. Elevated transcription of Ov-aqp-1 was evident in the egg, cercaria, metacercaria and adult stages of O. viverrini, whereas Ov-aqp-2 transcripts were detected at higher level in egg, metacercaria, cercaria and adult stage, respectively. RNA interference using electroporated dsRNA suppressed transcript levels of Ov-aqp-1 and Ov-aqp-2 in adult worms by 58-99% over periods of up to 16 days in vitro. Suppression of Ov-aqp-1 and Ov-aqp-2 in vitro disabled water transport in adult flukes. CONCLUSION:The apparently pivotal roles of Ov-AQP in solute homeostasis at the fluke surface suggest that deeper investigation will be informative for the pathophysiology of O. viverrini, and may uncover intervention targets, particularly in view of the singularly notable predilection of this pathogen for residence within ducts of the biliary tree.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Thanasuwan S,Piratae S,Brindley PJ,Loukas A,Kaewkes S,Laha Tdoi
10.1186/1756-3305-7-224subject
Has Abstractpub_date
2014-05-14 00:00:00pages
224issn
1756-3305pii
1756-3305-7-224journal_volume
7pub_type
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-48
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-52
更新日期:2012-03-20 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1962-y
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-262
更新日期:2014-06-05 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0913-3
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2625-y
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1528-z
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-4-12
更新日期:2011-02-03 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章,随机对照试验
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更新日期:2015-02-05 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-190
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2255-9
更新日期:2017-06-27 00:00:00
abstract:BACKGROUND:Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in centr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2398-8
更新日期:2017-10-02 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0581-8
更新日期:2014-12-16 00:00:00
abstract:BACKGROUND:Spirocerca lupi is a parasitic nematode of canids that can lead to a severe and potentially fatal disease. Recently, a new species, Spirocerca vulpis, was described from red foxes in Europe, suggesting a high genetic diversity of the Spirocerca spp. infecting canids. The genetic variation and phylogenetic re...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3202-0
更新日期:2018-12-14 00:00:00
abstract:BACKGROUND:Parasitic flatworms (Trematoda: Digenea) represent one of the most remarkable examples of drastic morphological diversity among the stages within a life cycle. Which genes are responsible for extreme differences in anatomy, physiology, behavior, and ecology among the stages? Here we report a comparative tran...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04424-4
更新日期:2020-11-10 00:00:00
abstract:BACKGROUND:Schistosomiasis primarily affects poor and neglected communities due to their lack of safe water and sanitation facilities. In an effort to improve intervention strategies, the present study investigated the association of socio-demographic characteristics of women with their existing knowledge, perceptions ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3668-4
更新日期:2019-09-23 00:00:00
abstract:BACKGROUND:Rats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short mitochondrial DNA regions is r...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2019-05-16 00:00:00