Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages.

Abstract:

BACKGROUND:Neospora caninum is an obligate intracellular parasite, and its ability to survive inside host immune cells may be a key mechanism for the establishment of infection in cattle. In vitro studies carried out by our group have shown that N. caninum is able to replicate in bovine macrophages (MØs), alter their microbicidal mechanisms and exploit their motility. Furthermore, host-cell control seems to be isolate virulence-dependent. METHODS:To investigate the molecular basis underlying the innate responses in MØs against N. caninum and the mechanisms of parasite manipulation of the host cell environment, the transcriptome profile of bovine monocyte-derived MØs infected with high-virulence (Nc-Spain7) or low-virulence (Nc-Spain1H) N. caninum isolates was studied. RESULTS:Functional enrichment revealed upregulation of genes involved in chemokine signalling, inflammation, cell survival, and inhibition of genes related with metabolism and phagolysosome formation. MØs activation was characterized by the induction of a predominantly M1 phenotype with expression of TLR2, TLR3 and TLR9 and activation of the NF-ƙB signalling pathway. Heat-killed N. caninum tachyzoites failed to activate NF-ƙB, and to inhibit lysosomal activity and apoptosis, which indicates active modulation by the parasite. The FoxO signalling pathway, Th1-Th2 differentiation, glycosaminoglycan degradation and apoptosis were pathways enriched only for low virulent Nc-Spain1H infection. In addition, Nc-Spain1H infection upregulated the IL12A and IL8 pro-inflammatory cytokines, whereas IL23 was downregulated by high virulent Nc-Spain7. CONCLUSIONS:This study revealed mechanisms implicated in the recognition of N. caninum by bovine MØs and in the development of the subsequent immune response. NF-ƙB seems to be the main signalling pathway implicated in the pro-inflammatory bovine MØs response against this pathogen. Apoptosis and phagolysosome maturation are processes repressed by N. caninum infection, which may guarantee its intracellular survival. The results also indicate that Nc-Spain7 may be able to partially circumvent the pro-inflammatory response whereas Nc-Spain1H induces a protective response to infection, which may explain the more efficient transmission of the high-virulence Nc-Spain7 isolate observed in vivo.

journal_name

Parasit Vectors

journal_title

Parasites & vectors

authors

García-Sánchez M,Jiménez-Pelayo L,Horcajo P,Collantes-Fernández E,Ortega-Mora LM,Regidor-Cerrillo J

doi

10.1186/s13071-020-04239-3

subject

Has Abstract

pub_date

2020-07-25 00:00:00

pages

374

issue

1

issn

1756-3305

pii

10.1186/s13071-020-04239-3

journal_volume

13

pub_type

杂志文章
  • HisAK70: progress towards a vaccine against different forms of leishmaniosis.

    abstract:BACKGROUND:Leishmania major and Leishmania infantum are among the main species that are responsible for cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL), respectively. The leishmanioses represent the second-largest parasitic killer in the world after malaria. Recently, we succeeded in generating a plasmid D...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-015-1246-y

    authors: Domínguez-Bernal G,Horcajo P,Orden JA,Ruiz-Santa-Quiteria JA,De La Fuente R,Ordóñez-Gutiérrez L,Martínez-Rodrigo A,Mas A,Carrión J

    更新日期:2015-12-09 00:00:00

  • Evaluation of resting traps to examine the behaviour and ecology of mosquito vectors in an area of rapidly changing land use in Sabah, Malaysian Borneo.

    abstract:BACKGROUND:Widespread deforestation occurring in the tropics is hypothesized to impact the transmission of vector-borne diseases (VBD). Predicting how environmental changes will impact VBD transmission is dependent on understanding the ecology and behaviour of potential vector species outside of domestic settings. Howe...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2926-1

    authors: Brown R,Hing CT,Fornace K,Ferguson HM

    更新日期:2018-06-14 00:00:00

  • Rapid on-site diagnosis of canine giardiosis: time versus performance.

    abstract:BACKGROUND:Infections by protozoans of the genus Giardia are a common cause of diarrhea in dogs. Canine giardiosis constitutes a disease with a zoonotic potential; however, it is often underestimated due to its challenging diagnosis. The objective of the study was to assess the diagnostic performance of an immunochroma...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-020-04422-6

    authors: Symeonidou I,Gelasakis AΙ,Miliotou AN,Angelou A,Arsenopoulos KV,Loukeri S,Papadopoulos E

    更新日期:2020-11-02 00:00:00

  • Resistance to pyrethroid and organophosphate insecticides, and the geographical distribution and polymorphisms of target-site mutations in voltage-gated sodium channel and acetylcholinesterase 1 genes in Anopheles sinensis populations in Shanghai, China.

    abstract:BACKGROUND:In the final phase of China's national programme to eliminate malaria by 2020, it is vitally important to monitor the resistance of malaria vectors for developing effective vector control strategies. In 2017 Shanghai declared that it had eliminated malaria; however, the insecticide resistance status of the p...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-019-3657-7

    authors: Fang Y,Shi WQ,Wu JT,Li YY,Xue JB,Zhang Y

    更新日期:2019-08-09 00:00:00

  • Insecticidal effects of deltamethrin in laboratory and field populations of Culicoides species: how effective are host-contact reduction methods in India?

    abstract:BACKGROUND:Bluetongue virus (BTV) is transmitted by Culicoides biting midges and causes bluetongue (BT), a clinical disease observed primarily in sheep. BT has a detrimental effect on subsistence farmers in India, where hyperendemic outbreaks impact on smallholdings in the southern states of the country. In this study,...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-1992-0

    authors: De Keyser R,Cassidy C,Laban S,Gopal P,Pickett JA,Reddy YK,Prasad M,Prasad G,Chirukandoth S,Senthilven K,Carpenter S,Logan JG

    更新日期:2017-01-31 00:00:00

  • Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays.

    abstract:BACKGROUND:The diagnosis of cutaneous leishmaniasis (CL) might be difficult, in particular in endemic areas where different species of Leishmania can cause lesions of very similar appearance and where other skin diseases with similar clinical symptoms occur. Even today, the parasitological diagnosis of CL remains the g...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-7-420

    authors: Mouttaki T,Morales-Yuste M,Merino-Espinosa G,Chiheb S,Fellah H,Martin-Sanchez J,Riyad M

    更新日期:2014-09-04 00:00:00

  • Canine leishmaniosis in South America.

    abstract:: Canine leishmaniosis is widespread in South America, where a number of Leishmania species have been isolated or molecularly characterised from dogs. Most cases of canine leishmaniosis are caused by Leishmania infantum (syn. Leishmania chagasi) and Leishmania braziliensis. The only well-established vector of Leishmani...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-2-S1-S1

    authors: Dantas-Torres F

    更新日期:2009-03-26 00:00:00

  • Anaplasma spp. in dogs and owners in north-western Morocco.

    abstract:BACKGROUND:Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2148-y

    authors: Elhamiani Khatat S,Daminet S,Kachani M,Leutenegger CM,Duchateau L,El Amri H,Hing M,Azrib R,Sahibi H

    更新日期:2017-04-24 00:00:00

  • Modeling the effects of palm-house proximity on the theoretical risk of Chagas disease transmission in a rural locality of the Orinoco basin, Colombia.

    abstract:BACKGROUND:Chagas disease is a major public health concern in Latin America and it is transmitted by insects of the subfamily Triatominae, including Rhodnius spp. Since palm trees are ubiquitous in Colombia and a habitat for Rhodnius spp., the presence of palms near villages could increase contact rates between vectors...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1884-8

    authors: Erazo D,Cordovez J

    更新日期:2016-11-18 00:00:00

  • Low genetic diversity of Ehrlichia canis associated with high co-infection rates in Rhipicephalus sanguineus (s.l.).

    abstract:BACKGROUND:Rhipicephalus sanguineus sensu lato (s.l.) is the most widely distributed ixodid tick and is a vector of major canine and human pathogens. High-throughput technologies have revealed that individual ticks carry a high diversity of pathogens, including bacteria, protozoa and viruses. Currently, it is accepted ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-3194-9

    authors: Cabezas-Cruz A,Allain E,Ahmad AS,Saeed MA,Rashid I,Ashraf K,Yousfi L,Shehzad W,Indjein L,Rodriguez-Valle M,Estrada-Peña A,Obregón D,Jabbar A,Moutailler S

    更新日期:2019-01-07 00:00:00

  • Molecular detection of Setaria tundra (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany.

    abstract:BACKGROUND:Knowledge of the potential vector role of Culicidae mosquitoes in Germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. However, anticipated changes in the course of global warming and globali...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-5-14

    authors: Czajka C,Becker N,Poppert S,Jöst H,Schmidt-Chanasit J,Krüger A

    更新日期:2012-01-11 00:00:00

  • Field evaluation of alphacypermethrin in indoor residual spraying for leishmaniasis control in an endemic area, northern Morocco.

    abstract:BACKGROUND:In Morocco, the main strategies of leishmaniasis vector control are based on environmental modifications. Use of local residual indoor spraying with synthetic pyrethroids is often envisaged. The need to evaluate this control method is essential. The current study was conducted to determine the efficacy of an...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-6-354

    authors: Faraj C,Adlaoui el B,Ouahabi S,Elkohli M,Elrhazi M,Laqraa L,Ameur B

    更新日期:2013-12-13 00:00:00

  • A perspective on the need and current status of efficient sex separation methods for mosquito genetic control.

    abstract::Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their envir...

    journal_title:Parasites & vectors

    pub_type: 杂志文章,评审

    doi:10.1186/s13071-018-3222-9

    authors: Papathanos PA,Bourtzis K,Tripet F,Bossin H,Virginio JF,Capurro ML,Pedrosa MC,Guindo A,Sylla L,Coulibaly MB,Yao FA,Epopa PS,Diabate A

    更新日期:2018-12-24 00:00:00

  • Identification of a novel β-adrenergic octopamine receptor-like gene (βAOR-like) and increased ATP-binding cassette B10 (ABCB10) expression in a Rhipicephalus microplus cell line derived from acaricide-resistant ticks.

    abstract:BACKGROUND:The cattle tick Rhipicephalus (Boophilus) microplus is an economically important parasite of livestock. Effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. Several continuous R. microplus cell lines have been established and provide an under-ut...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1708-x

    authors: Koh-Tan HH,Strachan E,Cooper K,Bell-Sakyi L,Jonsson NN

    更新日期:2016-08-02 00:00:00

  • Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of fasciolosis in sheep and its application under field conditions.

    abstract:BACKGROUND:Loop-mediated isothermal amplification (LAMP) is a very specific, efficient, and rapid gene amplification procedure in which the reaction can run at a constant temperature. In the current study we have developed a LAMP assay to improve the diagnosis of Fasciola spp. in the faeces of sheep. FINDINGS:After th...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1355-2

    authors: Martínez-Valladares M,Rojo-Vázquez FA

    更新日期:2016-02-05 00:00:00

  • First identification and multilocus genotyping of Giardia duodenalis in pet chipmunks (Eutamias asiaticus) in Sichuan Province, southwestern China.

    abstract:BACKGROUND:Giardia duodenalis is a flagellated parasite that causes diarrhea in humans and other animals. Although G. duodenalis is found in companion animals worldwide, information regarding the prevalence and genetic characteristics of G. duodenalis in pet chipmunks in China is limited. The present study therefore ai...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2790-z

    authors: Deng L,Luo R,Liu H,Zhou Z,Li L,Chai Y,Yang L,Wang W,Fu H,Zhong Z,Cao S,Peng G

    更新日期:2018-03-20 00:00:00

  • Morphological and molecular characterization and phylogenetic relationships of a new species of trypanosome in Tapirus terrestris (lowland tapir), Trypanosoma terrestris sp. nov., from Atlantic Rainforest of southeastern Brazil.

    abstract:BACKGROUND:The Lowland tapir (Tapirus terrestris) is the largest Brazilian mammal and despite being distributed in various Brazilian biomes, it is seriously endangered in the Atlantic Rainforest. These hosts were never evaluated for the presence of Trypanosoma parasites. METHODS:The Lowland tapirs were captured in the...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-6-349

    authors: Acosta Ida C,da Costa AP,Nunes PH,Gondim MF,Gatti A,Rossi JL Jr,Gennari SM,Marcili A

    更新日期:2013-12-11 00:00:00

  • Abiotic predictors and annual seasonal dynamics of Ixodes ricinus, the major disease vector of Central Europe.

    abstract:BACKGROUND:Abiotic conditions provide cues that drive tick questing activity. Defining these cues is critical in predicting biting risk, and in forecasting climate change impacts on tick populations. This is particularly important for Ixodes ricinus nymphs, the vector of numerous pathogens affecting humans. METHODS:A ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-015-1092-y

    authors: Daniel M,Malý M,Danielová V,Kříž B,Nuttall P

    更新日期:2015-09-18 00:00:00

  • Molecular fingerprinting of the myxozoan community in common carp suffering swim bladder inflammation (SBI) identifies multiple etiological agents.

    abstract:BACKGROUND:Swim bladder inflammation (SBI) is an important disease of common carp fingerlings in Central Europe. In the 1980s, its etiology was ascribed to multicellular proliferative stages of the myxozoan parasite Sphaerospora dykovae (formerly S. renicola). S. dykovae was reported to proliferate in the blood and in ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-7-398

    authors: Holzer AS,Hartigan A,Patra S,Pecková H,Eszterbauer E

    更新日期:2014-08-28 00:00:00

  • A multi-event capture-recapture analysis of Toxoplasma gondii seroconversion dynamics in farm cats.

    abstract:BACKGROUND:Domestic cats play a key role in the epidemiology of the parasite Toxoplasma gondii by excreting environmentally-resistant oocysts that may infect humans and other warm-blooded animals. The dynamics of Toxoplasma gondii seroconversion, used as a proxy for primo-infection dynamics, was investigated in five ca...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2834-4

    authors: Simon JA,Pradel R,Aubert D,Geers R,Villena I,Poulle ML

    更新日期:2018-06-08 00:00:00

  • Assessing the burden of intestinal parasites affecting newly arrived immigrants in Qatar.

    abstract:BACKGROUND:In the last decades, the enormous influx of immigrants to industrialized countries has led to outbreaks of parasitic diseases, with enteric infections being amongst the most frequently encountered. In its strategy to control such infection, Qatar has established the Pre-Employment Certificate (PEC) program w...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-016-1906-6

    authors: Abu-Madi MA,Behnke JM,Ismail A,Boughattas S

    更新日期:2016-12-01 00:00:00

  • Multi-function oxidases are responsible for the synergistic interactions occurring between repellents and insecticides in mosquitoes.

    abstract:BACKGROUND:With the spread of pyrethroid resistance in mosquitoes, the combination of an insecticide (carbamate or organophosphate) with a repellent (DEET) is considered as a promising alternative strategy for the treatment of mosquito nets and other relevant materials. The efficacy of these mixtures comes from the fac...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-2-17

    authors: Bonnet J,Pennetier C,Duchon S,Lapied B,Corbel V

    更新日期:2009-04-16 00:00:00

  • Integration of schistosomiasis control activities within the primary health care system: a critical review.

    abstract:BACKGROUND:Schistosomiasis is a chronic disease linked to poverty and is widely endemic, particularly in sub-Saharan Africa. For decades, the World Health Organization has called for a larger role of the primary health care system in schistosomiasis control, and its integration within the routine activities of primary ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章,评审

    doi:10.1186/s13071-019-3652-z

    authors: Bizimana P,Ortu G,Van Geertruyden JP,Nsabiyumva F,Nkeshimana A,Muhimpundu E,Polman K

    更新日期:2019-08-07 00:00:00

  • Genetic diversity, natural selection and haplotype grouping of Plasmodium vivax Duffy-binding protein genes from eastern and western Myanmar borders.

    abstract:BACKGROUND:Merozoite proteins of the malaria parasites involved in the invasion of red blood cells are selected by host immunity and their diversity is greatly influenced by changes in malaria epidemiology. In the Greater Mekong Subregion (GMS), malaria transmission is concentrated along the international borders and t...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-019-3803-2

    authors: Hu Y,Wang L,Mbenda HGN,Soe MT,Yu C,Feng H,Kyaw MP,Cui L,Zhu X,Cao Y

    更新日期:2019-11-20 00:00:00

  • Molecular prevalence of trypanosome infections in cattle and tsetse flies in the Maasai Steppe, northern Tanzania.

    abstract:BACKGROUND:African trypanosomosis is a disease of public health and economic importance that poses a major threat to the livelihoods of people living in the Maasai Steppe, where there is a significant interaction between people, livestock and wildlife. The vulnerability of the Maasai people to the disease is enhanced b...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-017-2411-2

    authors: Simwango M,Ngonyoka A,Nnko HJ,Salekwa LP,Ole-Neselle M,Kimera SI,Gwakisa PS

    更新日期:2017-10-23 00:00:00

  • Genome-wide identification and characterization of transfer RNA-derived small RNAs in Plasmodium falciparum.

    abstract:BACKGROUND:Transfer RNA (tRNA)-derived fragments (tRFs) have been widely identified in nature, functioning in diverse biological and pathological situations. Yet, the presence of these small RNAs in Plasmodium spp. remains unknown. Systematic identification and characterization of tRFs is therefore highly needed to und...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-019-3301-6

    authors: Wang Z,Wei C,Hao X,Deng W,Zhang L,Wang Z,Wang H

    更新日期:2019-01-15 00:00:00

  • Cold storage and cryopreservation of tick cell lines.

    abstract:BACKGROUND:Tick cell lines are now available from fifteen ixodid and argasid species of medical and veterinary importance. However, some tick cell lines can be difficult to cryopreserve, and improved protocols for short- and long-term low temperature storage will greatly enhance their use as tools in tick and tick-born...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/1756-3305-3-37

    authors: Lallinger G,Zweygarth E,Bell-Sakyi L,Passos LM

    更新日期:2010-04-13 00:00:00

  • Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts.

    abstract:BACKGROUND:Acanthamoebiasis is most often found in patients with immune deficiency, with infections facilitated by the intake of immunosuppressive drugs. The host immune response to Acanthamoeba spp. infection is poorly understood. Thus, in this study, we aimed to examine the course of Acanthamoeba spp. infection takin...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-3108-x

    authors: Łanocha-Arendarczyk N,Kolasa-Wołosiuk A,Wojciechowska-Koszko I,Kot K,Roszkowska P,Krasnodębska-Szponder B,Paczkowska E,Machaliński B,Łuczkowska K,Wiszniewska B,Kosik-Bogacka D

    更新日期:2018-09-20 00:00:00

  • Modelling the impact of larviciding on the population dynamics and biting rates of Simulium damnosum (s.l.): implications for vector control as a complementary strategy for onchocerciasis elimination in Africa.

    abstract:BACKGROUND:In 2012, the World Health Organization set goals for the elimination of onchocerciasis transmission by 2020 in selected African countries. Epidemiological data and mathematical modelling have indicated that elimination may not be achieved with annual ivermectin distribution in all endemic foci. Complementary...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-018-2864-y

    authors: Routledge I,Walker M,Cheke RA,Bhatt S,Nkot PB,Matthews GA,Baleguel D,Dobson HM,Wiles TL,Basañez MG

    更新日期:2018-05-29 00:00:00

  • Evaluation of traps and lures for mosquito vectors and xenomonitoring of Wuchereria bancrofti infection in a high prevalence Samoan Village.

    abstract:BACKGROUND:Elimination of lymphatic filariasis (LF) in Samoa continues to be challenging despite multiple annual mass drug campaigns aimed at stopping transmission by reducing the prevalence and density of microfilaraemia. The persistence of transmission may be partly related to the highly efficient Aedes vectors. The ...

    journal_title:Parasites & vectors

    pub_type: 杂志文章

    doi:10.1186/s13071-015-0886-2

    authors: Hapairai LK,Plichart C,Naseri T,Silva U,Tesimale L,Pemita P,Bossin HC,Burkot TR,Ritchie SA,Graves PM,Melrose W,Joseph H

    更新日期:2015-05-28 00:00:00