Abstract:
BACKGROUND:Parasitic flatworms (Trematoda: Digenea) represent one of the most remarkable examples of drastic morphological diversity among the stages within a life cycle. Which genes are responsible for extreme differences in anatomy, physiology, behavior, and ecology among the stages? Here we report a comparative transcriptomic analysis of parthenogenetic and amphimictic generations in two evolutionary informative species of Digenea belonging to the family Psilostomatidae. METHODS:In this study the transcriptomes of rediae, cercariae and adult worm stages of Psilotrema simillimum and Sphaeridiotrema pseudoglobulus, were sequenced and analyzed. High-quality transcriptomes were generated, and the reference sets of protein-coding genes were used for differential expression analysis in order to identify stage-specific genes. Comparative analysis of gene sets, their expression dynamics and Gene Ontology enrichment analysis were performed for three life stages within each species and between the two species. RESULTS:Reference transcriptomes for P. simillimum and S. pseudoglobulus include 21,433 and 46,424 sequences, respectively. Among 14,051 orthologous groups (OGs), 1354 are common and specific for two analyzed psilostomatid species, whereas 13 and 43 OGs were unique for P. simillimum and S. pseudoglobulus, respectively. In contrast to P. simillimum, where more than 60% of analyzed genes were active in the redia, cercaria and adult worm stages, in S. pseudoglobulus less than 40% of genes had such a ubiquitous expression pattern. In general, 7805 (36.41%) and 30,622 (65.96%) of genes were preferentially expressed in one of the analyzed stages of P. simillimum and S. pseudoglobulus, respectively. In both species 12 clusters of co-expressed genes were identified, and more than a half of the genes belonging to the reference sets were included into these clusters. Functional specialization of the life cycle stages was clearly supported by Gene Ontology enrichment analysis. CONCLUSIONS:During the life cycles of the two species studied, most of the genes change their expression levels considerably, consequently the molecular signature of a stage is not only a unique set of expressed genes, but also the specific levels of their expression. Our results indicate unexpectedly high level of plasticity in gene regulation between closely related species. Transcriptomes of P. simillimum and S. pseudoglobulus provide high quality reference resource for future evolutionary studies and comparative analyses.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Nesterenko MA,Starunov VV,Shchenkov SV,Maslova AR,Denisova SA,Granovich AI,Dobrovolskij AA,Khalturin KVdoi
10.1186/s13071-020-04424-4subject
Has Abstractpub_date
2020-11-10 00:00:00pages
559issue
1issn
1756-3305pii
10.1186/s13071-020-04424-4journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Schistosomiasis japonica remains a major public health concern in China. There are many interventions implemented to control the transmission of the disease. The purpose of the present study was to investigate the effects of an integrated control strategy for schistosomiasis control. METHODS:An integrated c...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-243
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:Chagas disease, caused by the parasite Trypanosoma cruzi, is mainly transmitted by blood-sucking bugs called triatomines. In the Yucatán Peninsula, Mexico, the main vector of T. cruzi is Triatoma dimidiata. While this species may colonize houses in other regions, it is mostly intrusive in Yucatán: it general...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1852-3
更新日期:2016-11-03 00:00:00
abstract:BACKGROUND:Porcine cysticercosis is an emerging agricultural problem in sub-Saharan Africa. This has been documented primarily through cross-sectional studies, however detailed knowledge of the transmission dynamics of this disease in sub-Saharan Africa is lacking. This study aims to describe seasonal variations in ser...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0574-7
更新日期:2014-12-04 00:00:00
abstract:BACKGROUND:Lymphatic filariasis (LF) due to Wuchereria bancrofti is being eliminated from Oceania under the Pacific Elimination of Lymphatic Filariasis Programme. LF was endemic in Solomon Islands but in the 2010-2020 Strategic Plan of the Global Programme to Eliminate LF, Solomon Islands was listed as non-endemic for ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-218
更新日期:2013-07-26 00:00:00
abstract:BACKGROUND:A plethora of evidence shows that activated microglia play a critical role in the pathogenesis of the central nervous system (CNS). Toxoplasmic encephalitis (TE) frequently occurs in HIV/AIDS patients. However, knowledge remains limited on the contributions of activated microglia to the pathogenesis of TE. ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-372
更新日期:2014-08-15 00:00:00
abstract:BACKGROUND:The emergence and spread of insecticide resistance in the major African malaria vectors Anopheles gambiae (s.s.) and An. arabiensis may compromise the current vector control interventions and threatens the global malaria control and elimination efforts. METHODS:Insecticide resistance was monitored in severa...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2342-y
更新日期:2017-09-02 00:00:00
abstract:BACKGROUND:Antigen B (EgAgB) is an abundant lipoprotein released by the larva of the cestode Echinococcus granulosus into the host tissues. Its protein moiety belongs to the cestode-specific family known as hydrophobic ligand binding protein (HLBP), and is encoded by five gene subfamilies (EgAgB8/1-EgAgB8/5). The funct...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1350-7
更新日期:2016-02-04 00:00:00
abstract:BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0608-1
更新日期:2015-01-06 00:00:00
abstract:BACKGROUND:In the Eastern and Upper Midwestern regions of North America, Ixodes scapularis (L.) is the most abundant tick species encountered by humans and the primary vector of B. burgdorferi, whereas in the southeastern region Amblyomma americanum (Say) is the most abundant tick species encountered by humans but cann...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-139
更新日期:2012-07-10 00:00:00
abstract:BACKGROUND:Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, causing quite pronounced...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3429-4
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:Cytauxzoonosis is an emerging tick-borne disease of domestic and wild felids. Cytauxzoon felis induces severe and often fatal disease in domestic cats. In Europe, clinical and subclinical infections caused by Cytauxzoon sp. are described. We report the first cases of Cytauxzoon sp. infection in domestic cats...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2728-5
更新日期:2018-05-10 00:00:00
abstract:BACKGROUND:Liver fibrosis is an excessive wound-healing reaction that requires the participation of inflammatory cells and hepatic stellate cells (HSCs). The pathogenesis of liver fibrosis caused by viruses and alcohol has been well characterized, but the molecular mechanisms underlying liver fibrosis induced by the li...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2228-z
更新日期:2017-06-17 00:00:00
abstract:BACKGROUND:Over 60 % of human emerging infectious diseases are zoonotic, and there is growing evidence of the zooanthroponotic transmission of diseases from humans to livestock and wildlife species, with major implications for public health, economics, and conservation. Zooanthroponoses are of relevance to critically e...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2283-5
更新日期:2017-07-18 00:00:00
abstract:BACKGROUND:The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We report on the cloning and expression of a recombinant F. gigantica 14-3-...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2745-4
更新日期:2018-03-06 00:00:00
abstract:BACKGROUND:Cystic echinococcosis (CE) is a near cosmopolitan zoonosis caused by the larval stage of the dog tapeworm Echinococcus granulosus. E. granulosus infection induces a polarized T-helper type 2 (Th2) systematic immune response in its intermediate hosts. However, it is not known whether the infection modulates l...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0522-6
更新日期:2014-11-20 00:00:00
abstract:BACKGROUND:The host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. Although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1659-2
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Rats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short mitochondrial DNA regions is r...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3491-y
更新日期:2019-05-16 00:00:00
abstract:BACKGROUND:A cytogenetic analysis of the new local triploid population of the caryophyllidean tapeworm Atractolytocestus huronensis, a unique parthenogenetic species with the ability to colonise new regions, was performed to understand the inner structure of its chromosome complement. METHODS:A karyotype analysis was ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3420-0
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Ixodes ricinus constitutes the main European vector tick for the Lyme borreliosis pathogen Borrelia burgdorferi (sensu lato), the relapsing fever borrelia Borrelia miyamotoi, as well as Anaplasma phagocytophilum and several Rickettsia species. Under laboratory conditions, a transovarial transmission to the n...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04049-7
更新日期:2020-04-07 00:00:00
abstract:BACKGROUND:Taxonomic identification of ticks obtained during a longitudinal survey of the critically endangered marsupial, Bettongia penicillata Gray, 1837 (woylie, brush-tailed bettong) revealed a new species of Ixodes Latrielle, 1795. Here we provide morphological data for the female and nymphal life stages of this n...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-1997-8
更新日期:2017-02-07 00:00:00
abstract:BACKGROUND:Functional analysis of animal parasitic nematode genes is often quite challenging due to the unavailability of standardised in vitro culture conditions and lack of adequate tools to manipulate these genes. Therefore, this study was undertaken to investigate the suitability of Culex quinquefasciatus, as an in...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3096-x
更新日期:2018-10-10 00:00:00
abstract:BACKGROUND:With field releases starting in Brazil, particular interest must be given to understanding how the endosymbiotic bacterium Wolbachia pipientis affects Aedes aegypti mosquitoes with a Brazilian genetic background. Currently, there is limited information on how the bacterium affects phenotypic traits such as l...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1559-5
更新日期:2016-05-16 00:00:00
abstract:BACKGROUND:A microsporidian was previously reported to infect the crustacean parasite, Lepeophtheirus salmonis (Krøyer, 1837) (Copepoda, Caligidae), on farmed Atlantic salmon (Salmo salar L.) in Scotland. The microsporidian was shown to be a novel species with a molecular phylogenetic relationship to Nucleospora (Enter...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-2-58
更新日期:2009-11-27 00:00:00
abstract::Since the implementation of Roll Back Malaria, the widespread use of insecticide-treated nets (ITNs) and indoor residual spraying (IRS) is thought to have played a major part in the decrease in mortality and morbidity achieved in malaria-endemic regions. In the past decade, resistance to major classes of insecticides ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-020-04170-7
更新日期:2020-06-10 00:00:00
abstract:BACKGROUND:Cryptosporidiosis is a gastrointestinal disease affecting many people worldwide. Disease incidence is often unknown and surveillance of human cryptosporidiosis is installed in only a handful of developed countries. A genetic marker that mirrors disease incidence is potentially a powerful tool for monitoring ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0921-3
更新日期:2015-06-06 00:00:00
abstract:BACKGROUND:Many cases of cutaneous leishmaniasis (CL) have been recorded in the Jenin District based on their clinical appearance. Here, their parasites have been characterized in depth. METHODS:Leishmanial parasites isolated from 12 human cases of CL from the Jenin District were cultured as promastigotes, whose DNA w...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-121
更新日期:2012-06-18 00:00:00
abstract:BACKGROUND:Japan is one of the few countries believed to have eliminated soil-transmitted helminths (STHs). In 1949, the national prevalence of Ascaris lumbricoides was 62.9%, which decreased to 0.6% in 1973 due to improvements in infrastructure, socioeconomic status, and the implementation of national STH control meas...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3875-z
更新日期:2020-01-08 00:00:00
abstract:BACKGROUND:In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, B...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1963-x
更新日期:2017-01-24 00:00:00
abstract:BACKGROUND:This laboratory study compared the efficacy of Bravecto™ (fluralaner), formulated as a chewable tablet, with the efficacy of Advocate® (imidacloprid/moxidectin), formulated for topical administration, against naturally acquired generalized demodicosis in dogs. METHODS:Sixteen dogs, all diagnosed with genera...
journal_title:Parasites & vectors
pub_type: 杂志文章,随机对照试验
doi:10.1186/s13071-015-0775-8
更新日期:2015-03-28 00:00:00
abstract:BACKGROUND:Henneguya Thélohan, 1892 (Myxobolidae) is one of the most species-rich genera of myxosporean parasites infecting fish. Although common in nature, there are few reports of these parasites causing important disease in aquaculture. In this paper, we describe a new species of Henneguya infecting Pagrus major (Te...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3888-7
更新日期:2020-01-14 00:00:00