Abstract：
:Mosquito salivary proteins are involved in several biological processes that facilitate their blood feeding and have also been reported to elicit an IgG response in vertebrates. A growing number of studies have focused on this immunological response for its potential use as a biological marker of exposure to arthropod bites. As mosquito saliva collection is extremely laborious and inefficient, most research groups prefer to work on mosquito salivary glands (SGs). Thus, SG protein integrity is a critical factor in obtaining meaningful data from immunological and biochemical analysis. Current methodologies rely on an immediate freezing of SGs after their collection. However, the maintenance of samples in a frozen environment can be hard to achieve in field conditions. In this study, SG proteins from two mosquito species (Aedes aegypti and Anopheles gambiae s.s.) stored in different media for 5 days at either +4°C or room temperature (RT) were evaluated at the quantitative (i.e., ELISA) and qualitative (i.e., SDS-PAGE and immunoblotting) levels. Our results indicated that PBS medium supplemented with an anti-protease cocktail seems to be the best buffer to preserve SG antigens for 5 days at +4°C for ELISA analysis. Conversely, cell-lysis buffer (Urea-Thiourea-CHAPS-Tris) was best at preventing protein degradation both at +4°C and RT for further qualitative analysis. These convenient storage methods provide an alternative to freezing and are expected to be applicable to other biological samples collected in the field.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Fontaine A,Pascual A,Diouf I,Bakkali N,Bourdon S,Fusai T,Rogier C,Almeras Ldoi
10.1186/1756-3305-4-33subject
Has Abstractpub_date
2011-03-08 00:00:00pages
33issn
1756-3305pii
1756-3305-4-33journal_volume
4pub_type
杂志文章abstract：BACKGROUND:Chagas disease control programmes have decreased the prevalence of Chagas disease in Latin America. Together with migration to urban areas and increase in life expectancy, a new scenario for Chagas disease has emerged in Brazil with most patients currently elderly individuals living in urban areas. However, ...
journal_title：Parasites & vectors
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更新日期：2018-06-19 00:00:00
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更新日期：2016-02-02 00:00:00
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pub_type： 已发布勘误
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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更新日期：2016-02-24 00:00:00
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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更新日期：2011-07-06 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章,随机对照试验
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更新日期：2014-07-29 00:00:00
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章,评审
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更新日期：2011-12-13 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
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更新日期：2015-09-04 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-86
更新日期：2010-09-15 00:00:00
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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更新日期：2014-09-04 00:00:00
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journal_title：Parasites & vectors
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更新日期：2015-12-30 00:00:00
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journal_title：Parasites & vectors
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更新日期：2019-09-11 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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更新日期：2011-11-14 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-82
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abstract：BACKGROUND:Animal trypanosomosis is endemic in Nigeria, while the human disease caused by Trypanosoma brucei gambiense is rarely reported nowadays after efforts to bring it under control in the 20th century. The University of Nigeria Veterinary Teaching Hospital (UNVTH) is a reference centre located within the Nsukka a...
journal_title：Parasites & vectors
pub_type： 杂志文章
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更新日期：2019-08-27 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
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更新日期：2019-09-16 00:00:00
abstract：BACKGROUND:Giardia duodenalis is a flagellated parasite that causes diarrhea in humans and other animals. Although G. duodenalis is found in companion animals worldwide, information regarding the prevalence and genetic characteristics of G. duodenalis in pet chipmunks in China is limited. The present study therefore ai...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2790-z
更新日期：2018-03-20 00:00:00
