Abstract：
BACKGROUND:Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic areas. Recombinase Polymerase Amplification (RPA) is an isothermal DNA amplification/detection technology that is simple, rapid, portable and needs few resources. FINDINGS:Here a Schistosoma haematobium RPA assay was developed and adapted so that DNA amplicons could be detected using oligochromatographic Lateral Flow (LF) strips. The assay successfully amplified S. haematobium DNA at 30-45 °C in 10 mins and was sensitive to a lower limit of 100 fg of DNA. The assay was also successful with the addition of crude urine, up to 5% of the total reaction volume. Cross amplification occurred with other schistosome species but not with other common urine microorganisms. CONCLUSION:The LF-RPA assay developed here can amplify and detect low levels of S. haematobium DNA. Reactions are rapid, require low temperatures and positive reactions are interpreted using lateral flow strips, reducing the need for infrastructure and resources. This together with an ability to withstand inhibitors within urine makes RPA a promising technology for further development as a molecular diagnostic tool for urogenital schistosomiasis.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Rosser A,Rollinson D,Forrest M,Webster BLdoi
10.1186/s13071-015-1055-3subject
Has Abstractpub_date
2015-09-04 00:00:00pages
446issn
1756-3305pii
10.1186/s13071-015-1055-3journal_volume
8pub_type
杂志文章abstract：BACKGROUND:To reveal the spatio-temporal distribution of malaria vectors in the national malaria surveillance sites from 2005 to 2010 and provide reference for the current National Malaria Elimination Programme (NMEP) in China. METHODS:A 6-year longitudinal surveillance on density of malaria vectors was carried out in...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-0741-5
更新日期：2015-03-07 00:00:00
abstract：BACKGROUND:Anopheles mosquitoes transmit malaria which is one of the world's most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune d...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1810-0
更新日期：2016-09-29 00:00:00
abstract：BACKGROUND:Haemoproteus parasites (Haemosporida, Haemoproteidae) are cosmopolitan in birds and recent molecular studies indicate enormous genetic diversity of these pathogens, which cause diseases in non-adapted avian hosts. However, life-cycles remain unknown for the majority of Haemoproteus species. Information on th...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3773-4
更新日期：2019-11-04 00:00:00
abstract：BACKGROUND:Knowledge of insecticide resistance status in the main malaria vectors is an essential component of effective malaria vector control. This study presents the first evaluation of the status of insecticide resistance in Anopheles gambiae populations from Bangui, the Central African Republic. METHODS:Anopheles...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1965-8
更新日期：2017-01-13 00:00:00
abstract：BACKGROUND:One of the major issues concerning disease ecology and conservation is knowledge of the factors that influence the distribution of parasites and consequently disease outbreaks. This study aimed to investigate avian haemosporidian composition and the distribution of these parasites in three altitudinally sepa...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-139
更新日期：2013-05-07 00:00:00
abstract：BACKGROUND:The basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. According to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. Concerning fish, both phy...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-120
更新日期：2011-06-27 00:00:00
abstract：BACKGROUND:Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in centr...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2398-8
更新日期：2017-10-02 00:00:00
abstract：BACKGROUND:In 2012, the World Health Organization set goals for the elimination of onchocerciasis transmission by 2020 in selected African countries. Epidemiological data and mathematical modelling have indicated that elimination may not be achieved with annual ivermectin distribution in all endemic foci. Complementary...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2864-y
更新日期：2018-05-29 00:00:00
abstract：BACKGROUND:The tick midgut and salivary glands represent the primary organs for pathogen acquisition and transmission, respectively. Specifically, the midgut is the first organ to have contact with pathogens during the blood meal uptake, while salivary glands along with their secretions play a crucial role in pathogen ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3418-7
更新日期：2019-04-02 00:00:00
abstract：BACKGROUND:Lymphatic filariasis (LF) due to Wuchereria bancrofti is being eliminated from Oceania under the Pacific Elimination of Lymphatic Filariasis Programme. LF was endemic in Solomon Islands but in the 2010-2020 Strategic Plan of the Global Programme to Eliminate LF, Solomon Islands was listed as non-endemic for ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-218
更新日期：2013-07-26 00:00:00
abstract：BACKGROUND:Rickettsia spp. are obligate intracellular bacteria and well known as transmitted by arthropods. These pathogens have a broad geographic distribution and a high degree of biological and clinical diversity. This study was conducted to determine the prevalence and molecular characterization of Rickettsia spp. ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1423-7
更新日期：2016-03-12 00:00:00
abstract：BACKGROUND:Lymphatic filariasis (LF) is a debilitating disease associated with extensive disfigurement and is one of a diverse group of diseases referred to as neglected tropical diseases (NTDs) which mainly occur among the poorest populations. In line with global recommendations to eliminate LF, Kenya launched its LF ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2044-5
更新日期：2017-02-22 00:00:00
abstract：BACKGROUND:Because agriculture and offshore oil extraction are significant economic activities in the southern Gulf of Mexico, high concentrations of nutrients and hydrocarbons are expected. As parasite communities are sensitive to environmental impacts, these contaminants should have an effect on metrics such as speci...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0541-3
更新日期：2014-11-27 00:00:00
abstract：BACKGROUND:Filariasis and leishmaniasis are two neglected tropical diseases in Mali. Due to distribution and associated clinical features, both diseases are of concern to public health. The goal of this study was to determine the prevalence of co-infection with filarial (Wuchereria bancrofti and Mansonella perstans) an...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2531-8
更新日期：2018-01-08 00:00:00
abstract：BACKGROUND:Cutaneous Leishmaniasis (CL) is endemic in Sudan. It is caused by Leishmania major parasites and transmitted by Phlebotomus papatasi sandflies. Recently, uncommon clinical manifestations of CL have been reported. Moreover, L. donovani parasites that cause Visceral Leishmaniasis (VL) have been isolated from C...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-249
更新日期：2012-11-12 00:00:00
abstract：BACKGROUND:Animal African trypanosomiasis (AAT) greatly affects livestock production in sub-Saharan Africa. In Ghana prevalence of AAT is estimated to range between 5 and 50%. Studies have reported serum biochemical aberrations and variability in cytokine profiles in animals during infection. However, information regar...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2255-9
更新日期：2017-06-27 00:00:00
abstract：BACKGROUND:Insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. These viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. Following the isolation of the first Australian...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1683-2
更新日期：2016-07-25 00:00:00
abstract：BACKGROUND:The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylse...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2198-1
更新日期：2017-05-30 00:00:00
abstract：BACKGROUND:Anaplasma ovis is a gram-negative, tick-borne obligate intraerythrocytic pathogen, which causes ovine anaplasmosis in small ruminants worldwide. VirB10 of A. ovis is an integral component of the Type IV Secretion System (T4SS). The T4SS is used by bacteria to transfer DNA and/or proteins undeviatingly into t...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-3975-9
更新日期：2020-02-27 00:00:00
abstract：BACKGROUND:The survival of adult female Aedes mosquitoes is a critical component of their ability to transmit pathogens such as dengue viruses. One of the principal determinants of Aedes survival is temperature, which has been associated with seasonal changes in Aedes populations and limits their geographical distribut...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-351
更新日期：2013-12-12 00:00:00
abstract：BACKGROUND:Recent studies demonstrate that insect-specific viruses can influence the ability of their mosquito hosts to become infected with and transmit arboviruses of medical and veterinary importance. The aim of this study was to evaluate the interactions between Anopheles gambiae densovirus (AgDNV) (Parvoviridae) (...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04072-8
更新日期：2020-04-22 00:00:00
abstract：BACKGROUND:Equine piroplasmosis is a highly endemic protozoan disease of horses worldwide, caused by Theileria equi and Babesia caballi. While most horses in endemic areas are subclinically infected, the mechanisms leading to clinical outcome are vastly unknown. Moreover, since clinical signs of disease are not specifi...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04133-y
更新日期：2020-05-20 00:00:00
abstract：BACKGROUND:The abundance, richness and diversity of mosquitoes and aquatic insects associated with their oviposition sites were surveyed along eight states of the Pacific coast of Mexico. Diversity was estimated using the Shannon index (H'), similarity measures and cluster analysis. METHODS:Oviposition sites were samp...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-41
更新日期：2014-01-22 00:00:00
abstract：BACKGROUND:The efficacy of fluralaner for the treatment of Otodectes cynotis infestations in dogs and cats was evaluated after oral (dogs) or topical administration (dogs and cats). Twenty-four dogs and sixteen cats were experimentally infested with O. cynotis and randomly allocated to equal sized groups (n = 8/group)....
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1954-y
更新日期：2017-01-16 00:00:00
abstract：BACKGROUND:Novel transgenic mosquito control methods require progressively more realistic evaluation. The goal of this study was to determine the effect of a transgene that causes a male-bias sex ratio on Anopheles gambiae target populations in large insectary cages. METHODS:Life history characteristics of Anopheles g...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3289-y
更新日期：2019-02-06 00:00:00
abstract：BACKGROUND:The identification of Trypanosoma cruzi and blood-meal sources in synanthropic triatomines is important to assess the potential risk of Chagas disease transmission. We identified T. cruzi infection and blood-meal sources of triatomines caught in and around houses in the state of Bahia, northeastern Brazil, a...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3849-1
更新日期：2019-12-26 00:00:00
abstract：BACKGROUND:Studies of avian haemosporidians allow understanding how these parasites affect wild bird populations, and if their presence is related to factors such as habitat loss, degradation and fragmentation, and climate change. Considering the importance of the highland Plateau of Mexico as part of the North America...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1569-3
更新日期：2016-05-27 00:00:00
abstract：:Since 2004 there has been an increased recognition of the importance of Neglected Tropical Diseases (NTDs) as impediments to development. These diseases are caused by a variety of infectious agents - viruses, bacteria and parasites - which cause a diversity of clinical conditions throughout the tropics. The World Heal...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/1756-3305-4-234
更新日期：2011-12-13 00:00:00
abstract：BACKGROUND:Methods currently used in sampling adult Aedes aegypti, the main vector of dengue and chikungunya viruses are limited for effective surveillance of the vector and accurate determination of the extent of virus transmission during outbreaks and inter - epidemic periods. Here, we document the use of natural hum...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-451
更新日期：2014-09-23 00:00:00
abstract：BACKGROUND:The common cockle Cerastoderma edule plays an important ecological role in the marine ecosystem both as an infaunal engineer (reef forming and bioturbation) and a food source for protected bird species in its European range. Cockle beds are found in close proximity to aquaculture and fisheries operations, wh...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04373-y
更新日期：2020-10-01 00:00:00
