Abstract:
BACKGROUND:Anaplasma ovis is a gram-negative, tick-borne obligate intraerythrocytic pathogen, which causes ovine anaplasmosis in small ruminants worldwide. VirB10 of A. ovis is an integral component of the Type IV Secretion System (T4SS). The T4SS is used by bacteria to transfer DNA and/or proteins undeviatingly into the host cell to increase their virulence. To more thoroughly understand the interaction between A. ovis and Dermacentor silvarum, a vector containing the virb10 gene of A. ovis was used as a bait plasmid to screen interacting proteins from the cDNA library of the D. silvarum salivary gland using the yeast two-hybrid system. METHODS:The cDNA of the D. silvarum salivary gland was cloned into the pGADT7-SmaI vector (prey plasmid) to construct the yeast two-hybrid cDNA library. The virb10 gene was cloned into the pGBKT7 vector to generate a bait plasmid. Any gene auto-activation or toxicity effects in the yeast strain Y2HGold were excluded. The screening was performed by combining the bait and prey plasmids in yeast strains to identify positive preys. The positive preys were then sequenced, and the obtained sequences were subjected to further analyses using Gene Ontology, UniProt, SMART, and STRING. Additionally, the interaction between the bait and the prey was evaluated using the glutathione S-transferase (GST) pull-down assay. RESULTS:A total of two clones were obtained from the cDNA library using the yeast two-hybrid system, and the sequence analysis showed that both clones encoded the same large tegument protein, UL36. Furthermore, the proteins GST-UL36 and His-VirB10 were successfully expressed in vitro and the interaction between the two proteins was successfully demonstrated by the GST pull-down assay. CONCLUSIONS:To our knowledge, this study is the first to screen for D. silvarum salivary gland proteins that interact with A. ovis VirB10. The resulting candidate, UL36, is a multi-functional protein. Further investigations into the functionality of UL36 should be carried out, which might help in identifying novel prevention and treatment strategies for A. ovis infection. The present study provides a base for exploring and further understanding the interactions between A. ovis and D. silvarum.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Mukhtar MU,Iqbal N,Yang J,Niu Q,Zhao S,Li Z,Zhao Y,Rashid M,Chen Z,Guan G,Liu Z,Yin Hdoi
10.1186/s13071-020-3975-9subject
Has Abstractpub_date
2020-02-27 00:00:00pages
105issue
1issn
1756-3305pii
10.1186/s13071-020-3975-9journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Understanding the dispersal dynamics of invasive mosquito species is fundamental to improve vector surveillance and to target control efforts. Aedes albopictus has been deemed a poor flyer as its range of active dispersal is generally assumed to be limited to a few hundred metres, while laboratory studies su...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3837-5
更新日期:2019-12-12 00:00:00
abstract:BACKGROUND:Schistosomiasis is a neglected tropical disease and drug-repurposing is a useful strategy to fill its exhausted drug development pipeline. The ferrocenyl analogue of chloroquine, ferroquine, is an antimalarial in late-stage drug development. The aim of the present work was to study the antischistosomal activ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-424
更新日期:2014-09-04 00:00:00
abstract:BACKGROUND:Release of gene-drive mutants to suppress Anopheles mosquito reproduction is a promising method of malaria control. However, many scientific, regulatory and ethical questions remain before transgenic mosquitoes can be utilised in the field. At a behavioural level, gene-drive carrying mutants should be at lea...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04382-x
更新日期:2020-10-07 00:00:00
abstract:BACKGROUND:The preventive effect of fluralaner spot-on solution against transmission of Babesia canis by Dermacentor reticulatus ticks was evaluated. FINDINGS:Sixteen dogs, tested negative for B. canis by polymerase chain reaction (PCR) and immunofluorescence assay test (IFAT), were allocated to two study groups. On d...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1481-x
更新日期:2016-05-31 00:00:00
abstract:BACKGROUND:Nematodirus battus, unlike most other gastrointestinal nematodes, undergoes maturation to an infective larva within the egg. Historically, eggs were considered to require a period of chilling over winter followed by a period of temperature above 10 °C for synchronous hatching to occur (generally in spring). ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04368-9
更新日期:2020-09-29 00:00:00
abstract:BACKGROUND:Carboxylesterase overproduction is a frequently observed resistance mechanism of insects to organophosphate insecticides. As a major transmitter of human diseases, mosquitoes in the Culex pipiens complex have evolved 13 carboxylesterase alleles (Ester) that confer organophosphate resistance. Six alleles, Est...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-209
更新日期:2012-09-24 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-372
更新日期:2014-08-15 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0741-5
更新日期:2015-03-07 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3475-y
更新日期:2019-05-14 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Parasites & vectors
pub_type: 已发布勘误
doi:10.1186/s13071-020-04349-y
更新日期:2020-09-22 00:00:00
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journal_title:Parasites & vectors
pub_type: 历史文章,杂志文章,评审
doi:10.1186/1756-3305-6-334
更新日期:2013-11-22 00:00:00
abstract:BACKGROUND:Annual community-directed treatment with ivermectin (CDTI) have been carried out since 1999 in the Lekie division (central region of Cameroon where most cases of Loa-related post ivermectin severe adverse events were reported) as part of the joined activities of the African Programme for Onchocerciasis Contr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-283
更新日期:2013-09-27 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2495-8
更新日期:2017-11-02 00:00:00
abstract:BACKGROUND:Lymphatic filariasis (LF) is a debilitating disease associated with extensive disfigurement and is one of a diverse group of diseases referred to as neglected tropical diseases (NTDs) which mainly occur among the poorest populations. In line with global recommendations to eliminate LF, Kenya launched its LF ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2044-5
更新日期:2017-02-22 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3803-2
更新日期:2019-11-20 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2917-2
更新日期:2018-06-04 00:00:00
abstract:BACKGROUND:The myxosporean parasite Parvicapsula pseudobranchicola commonly infects farmed Atlantic salmon in northern Norway. Heavy infections are associated with pseudobranch lesions, runting and mortality in the salmon populations. The life-cycle of the parasite is unknown, preventing controlled challenge experiment...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2583-9
更新日期:2018-01-06 00:00:00
abstract:BACKGROUND:In the final phase of China's national programme to eliminate malaria by 2020, it is vitally important to monitor the resistance of malaria vectors for developing effective vector control strategies. In 2017 Shanghai declared that it had eliminated malaria; however, the insecticide resistance status of the p...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3657-7
更新日期:2019-08-09 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3375-1
更新日期:2019-03-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2597-3
更新日期:2018-01-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-420
更新日期:2014-09-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1564-8
更新日期:2016-05-31 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-1992-0
更新日期:2017-01-31 00:00:00
abstract:BACKGROUND:Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the serop...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1753-5
更新日期:2016-08-24 00:00:00
abstract:BACKGROUND:Toxocara canis is the causative agent of toxocariasis of humans and other animals. This parasitic nematode (roundworm) has a complex life cycle, in which substantial developmental changes and switches occur. As small non-coding RNAs (sRNAs) are key regulators of gene expression in a wide range of organisms, ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1508-3
更新日期:2016-04-23 00:00:00
abstract:BACKGROUND:The trypanosome diversity of the Brush-tailed Bettong (Bettongia penicillata), known locally as the woylie, has been further investigated. At a species level, woylies are critically endangered and have declined by 90% since 1999. The predation of individuals made more vulnerable by disease is thought to be t...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-121
更新日期:2013-04-26 00:00:00
abstract:: The proteasome inhibitor and anti-cancer drug bortezomib was tested for in vitro activity against bloodstream forms of Trypanosoma brucei. The concentrations of bortezomib required to reduce the growth rate by 50% and to kill all trypanosomes were 3.3 nM and 10 nM, respectively. In addition, bortezomib was 10 times m...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-2-29
更新日期:2009-07-07 00:00:00
abstract:BACKGROUND:Entomopathogenic fungi are potential candidates for use in integrated vector management and many isolates are compatible with synthetic and natural insecticides. Neem oil was tested separately and in combination with the entomopathogenic fungus Metarhizium anisopliae against larvae of the dengue vector Aedes...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1280-9
更新日期:2015-12-30 00:00:00
abstract::Echinococcus transmission is known to be affected by various environmental factors, which may be modified by human influence or natural events including global warming. Considerable population growth in the last fifty years in Ningxia Hui Autonomous Region (NHAR), the People's Republic of China (PRC), has led to drama...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-5-146
更新日期:2012-07-24 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-207
更新日期:2014-04-29 00:00:00