Abstract：
BACKGROUND:Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis. RESULTS:The protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses. CONCLUSIONS:Larval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Wang H,Li J,Guo B,Zhao L,Zhang Z,McManus DP,Wen H,Zhang Wdoi
10.1186/s13071-016-1687-ysubject
Has Abstractpub_date
2016-07-25 00:00:00pages
411issue
1issn
1756-3305pii
10.1186/s13071-016-1687-yjournal_volume
9pub_type
杂志文章abstract：BACKGROUND:Schistosomiasis is a chronic disease linked to poverty and is widely endemic, particularly in sub-Saharan Africa. For decades, the World Health Organization has called for a larger role of the primary health care system in schistosomiasis control, and its integration within the routine activities of primary ...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/s13071-019-3652-z
更新日期：2019-08-07 00:00:00
abstract：BACKGROUND:Following the recent discovery of the role of Anopheles rufipes Gough, 1910 in human malaria transmission in the northern savannah of Cameroon, we report here additional information on its feeding and resting habits and its susceptibility to the pyrethroid insecticide deltamethrin. METHODS:From 2011 to 2015...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2809-5
更新日期：2018-04-18 00:00:00
abstract：BACKGROUND:The mosquito Aedes aegypti, vector of dengue fever, is a target for control by entomopathogenic fungi. Recent studies by our group have shown the susceptibility of adult A. aegypti to fungal infection by Metarhizium anisopliae. This fungus is currently being tested under field conditions. However, it is unkn...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-91
更新日期：2011-05-26 00:00:00
abstract：BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0608-1
更新日期：2015-01-06 00:00:00
abstract：BACKGROUND:Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic areas. Recombinase Polymerase Amplific...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-1055-3
更新日期：2015-09-04 00:00:00
abstract：BACKGROUND:Rhipicephalus microplus is a hard tick that has a major impact on cattle health in tropical and subtropical regions because it feeds on cattle and is implicated in the transmission of pathogens that cause diseases such as bovine anaplasmosis and babesiosis. Presently, acaricides are used to control tick infe...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2081-0
更新日期：2017-03-23 00:00:00
abstract：BACKGROUND:Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. FINDINGS:Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were se...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-6-178
更新日期：2013-06-17 00:00:00
abstract：BACKGROUND:A good understanding of mosquito ecology is imperative for integrated vector control of malaria. In breeding sites, Anopheles larvae are concurrently exposed to predators and parasites. However, to our knowledge, there is no study on combined effects of predators and parasites on development and survival of ...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/s13071-020-3915-8
更新日期：2020-01-30 00:00:00
abstract：BACKGROUND:Resistance to chemical insecticides plus high morbidity rates have lead to rising interest in fungi as candidates for biocontrol agents of mosquito vectors. In most studies fungal infections have been induced by exposure of mosquitoes to various surfaces treated with conidia. In the present study eight Mexic...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-24
更新日期：2011-02-26 00:00:00
abstract：BACKGROUND:In Brazil, the autochthonous transmission of extra-Amazonian malaria occurs mainly in areas of the southeastern coastal Atlantic Forest, where Anopheles cruzii is the primary vector. In these locations, the population density of the mosquito varies with altitude (5-263 m above sea level), prompting us to hyp...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0581-8
更新日期：2014-12-16 00:00:00
abstract：BACKGROUND:Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. Wild animals play an...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-1130-9
更新日期：2015-10-08 00:00:00
abstract：BACKGROUND:Limited available sequence information has greatly impeded population genetics, phylogenetics and systematics studies in the subclass Acari (mites and ticks). Mitochondrial (mt) DNA is well known to provide genetic markers for investigations in these areas, but complete mt genomic data have been lacking for ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-340
更新日期：2014-07-22 00:00:00
abstract：BACKGROUND:Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. R...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3308-z
更新日期：2019-01-28 00:00:00
abstract：BACKGROUND:The TNF signaling pathway is involved in the regulation of many cellular processes (such as apoptosis and cell proliferation). Previous reports indicated the effect of human TNF-α on metabolism, physiology, gene expression and protein phosphorylation of the human parasite Schistosoma mansoni and suggested th...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04370-1
更新日期：2020-09-25 00:00:00
abstract：BACKGROUND:Over 60 % of human emerging infectious diseases are zoonotic, and there is growing evidence of the zooanthroponotic transmission of diseases from humans to livestock and wildlife species, with major implications for public health, economics, and conservation. Zooanthroponoses are of relevance to critically e...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2283-5
更新日期：2017-07-18 00:00:00
abstract：BACKGROUND:Eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The devel...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1756-2
更新日期：2016-08-23 00:00:00
abstract：BACKGROUND:Cats appear to be the primary reservoir host for Bartonella koehlerae, an alpha Proteobacteria that is most likely transmitted among cat populations by fleas (Ctenocephalides felis). Bartonella koehlerae has caused endocarditis in a dog and in one human patient from Israel, but other clinically relevant repo...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-76
更新日期：2010-08-24 00:00:00
abstract：BACKGROUND:Paratransgenesis is an approach to reducing arthropod vector competence using genetically modified symbionts. When applied to control of Chagas disease, the symbiont bacterium Rhodococcus rhodnii, resident in the gut lumen of the triatomine vector Rhodnius prolixus (Hemiptera: Reduviidae), is transformed to ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-77
更新日期：2011-05-14 00:00:00
abstract：BACKGROUND:The human helminth infections include ascariasis, trichuriasis, hookworm infections, schistosomiasis, lymphatic filariasis (LF) and onchocerciasis. It is estimated that almost 2 billion people worldwide are infected with helminths. Whilst the WHO treatment guidelines for helminth infections are mostly aimed ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2656-4
更新日期：2018-01-31 00:00:00
abstract：BACKGROUND:The emergence and spread of insecticide resistance in the major African malaria vectors Anopheles gambiae (s.s.) and An. arabiensis may compromise the current vector control interventions and threatens the global malaria control and elimination efforts. METHODS:Insecticide resistance was monitored in severa...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2342-y
更新日期：2017-09-02 00:00:00
abstract：BACKGROUND:The use of inland valley swamps for vegetable crop agriculture contributes to food security in urban and less urbanized settings in Africa. The impact of this agriculture on aggressive mosquitoes' diversity and malaria transmission in central Africa is poorly documented. This study is aimed at assessing the ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-0906-2
更新日期：2015-05-28 00:00:00
abstract：BACKGROUND:Clonorchiasis ranks among the most important food-borne parasitic diseases in China. However, due to low compliance to traditional fecal examination techniques in the general population and medical personnel, immunodiagnosis is expected. This study evaluated, in parallel, the performance of four immunodiagno...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2612-3
更新日期：2018-01-15 00:00:00
abstract：BACKGROUND:Sandfly-borne phleboviruses are present in North Africa where they can infect humans in regions where Leishmania infantum, the causative agent of zoonotic visceral leishmaniasis in the Western Mediterranean basin is present affecting both humans and dogs. We investigated the capacity of dogs to be used as se...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0476-8
更新日期：2014-10-12 00:00:00
abstract：BACKGROUND:Pyrethroid resistance has been slower to emerge in Anopheles arabiensis than in An. gambiae s.s and An. funestus and, consequently, studies are only just beginning to unravel the genes involved. Permethrin resistance in An. arabiensis in Lower Moshi, Tanzania has been linked to elevated levels of both P450 m...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-274
更新日期：2014-06-19 00:00:00
abstract：BACKGROUND:The argasid tick Ornithodoros erraticus is the vector of African swine fever virus and of several Borrelia species that cause human relapsing fever in the Iberian Peninsula. The tick midgut is part of the ectoparasite-host interface and expresses proteins that are vital for the survival of the tick. Midgut p...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-1148-z
更新日期：2015-10-12 00:00:00
abstract：BACKGROUND:In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, B...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1963-x
更新日期：2017-01-24 00:00:00
abstract：BACKGROUND:Malaria transmission in Cameroon is mediated by a plethora of vectors that are heterogeneously distributed across the country depending on the biotope. To effectively guide malaria control operations, regular update on the role of local Anopheles species is essential. Therefore, an entomological survey was c...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-262
更新日期：2014-06-05 00:00:00
abstract：BACKGROUND:Blastocystis spp. are the most prevalent intestinal eukaryotes identified in humans, with at least 17 genetic subtypes (ST) based on genes coding for the small-subunit ribosomal RNA (18S). It has been argued that the 18S gene should not be the marker of choice to discriminate between STs of these strains bec...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-3141-9
更新日期：2018-10-29 00:00:00
abstract：BACKGROUND:A question of epidemiological relevance in Chagas disease studies is to understand Trypanosoma cruzi transmission cycles and trace the origins of (re)emerging cases in areas under vector or disease surveillance. Conventional parasitological methods lack sensitivity whereas molecular approaches can fill in th...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3817-9
更新日期：2019-11-29 00:00:00
abstract：BACKGROUND:Lipoproteins are the major agonists of Wolbachia-dependent inflammatory pathogenesis in filariasis and a validated target for drug discovery. Here we characterise the abundance, localisation and serology of the Wolbachia lipoproteins: Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion S...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-014-0462-1
更新日期：2014-10-06 00:00:00
