Abstract:
BACKGROUND:The argasid tick Ornithodoros erraticus is the vector of African swine fever virus and of several Borrelia species that cause human relapsing fever in the Iberian Peninsula. The tick midgut is part of the ectoparasite-host interface and expresses proteins that are vital for the survival of the tick. Midgut proteins are therefore potential targets for drug and/or vaccine design aimed at the development of new strategies for tick control. Thus, the aim of this work was the characterization of the proteome of the O. erraticus midgut before and after a blood meal trying to elucidate the induced changes upon blood feeding. METHODS:Midgut tissues from unfed and engorged O. erraticus females were dissected and proteins were fractionated by centrifugation and SDS-PAGE, and the corresponding gel pieces analysed by LC-MS/MS. The identified proteins were classified according to their Protein Class and Molecular Function and the differences between fed and unfed specimens were analysed. RESULTS:Overall 555 tick proteins were identified: 414 in the midgut of the unfed specimens and 376 in the fed specimens, of which 235 were present in both groups. The proteins with catalytic, binding and structural functions were the most numerous and abundant, consistent with their role in the intracellular processing of the blood meal. The analysis of some groups of proteins putatively involved directly in blood meal digestion, including protein digestion (peptidase activity), iron metabolism, enzymes involved in oxidative stress and detoxification and membrane traffic and transport proteins, detected some differences between the fed and unfed ticks CONCLUSIONS:This work reports for the first time the collection and analysis of the midgut proteome of an argasid tick species and provides molecular information about the argasid machinery involved in blood digestion. This information represents a starting point for the identification and selection of new targets for the development of alternative control strategies.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Oleaga A,Obolo-Mvoulouga P,Manzano-Román R,Pérez-Sánchez Rdoi
10.1186/s13071-015-1148-zsubject
Has Abstractpub_date
2015-10-12 00:00:00pages
525issn
1756-3305pii
10.1186/s13071-015-1148-zjournal_volume
8pub_type
杂志文章abstract:BACKGROUND:Culicoides biting midges are biological vectors of arboviruses including bluetongue virus (BTV), Schmallenberg virus (SBV) and African horse sickness virus (AHSV). Zoos are home to a wide range of 'at risk' exotic and native species of animals. These animals have a high value both in monetary terms, conserva...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04018-0
更新日期:2020-03-16 00:00:00
abstract:BACKGROUND:Digenean trematodes are parasitic platyhelminths that use several hosts in their life cycles and are thereby embedded in various ecosystems affected by local environmental conditions. Their presence in a habitat will reflect the presence of different host species and, as such, they can serve as ecological in...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04536-x
更新日期:2021-01-12 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis (VL) is an important public health challenge in Brazil because of the high number of human and canine cases reported annually. Leishmania infantum is the etiological agent of VL and Lutzomyia longipalpis is its main vector. However, evidence suggests that this taxon constitutes a speci...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2211-8
更新日期:2017-05-30 00:00:00
abstract::In the more than 100 years since its discovery, our knowledge of Toxoplasma biology has improved enormously. The evolution of molecular biology, immunology and genomics has had profound influences on our understanding of this ubiquitous bug. However, it could be argued that in science today the adage "seeing is believ...
journal_title:Parasites & vectors
pub_type: 历史文章,杂志文章,评审
doi:10.1186/1756-3305-6-334
更新日期:2013-11-22 00:00:00
abstract:BACKGROUND:Culex quinquefasciatus is a hematophagous insect from the Culicidae family that feeds on the blood of humans, dogs, birds and livestock. This species transmits a wide variety of pathogens between humans and animals. The midgut environment is the first location of pathogen-vector interactions for blood-feedin...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0985-0
更新日期:2015-07-16 00:00:00
abstract:BACKGROUND:Diagnostics provide a means to measure progress toward disease elimination. Many countries in Africa are approaching elimination of onchocerciasis after successful implementation of mass drug administration programs as well as vector control. An understanding of how markers for infection such as skin snip mi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1623-1
更新日期:2016-06-13 00:00:00
abstract:BACKGROUND:Neorickettsia are a group of intracellular α proteobacteria transmitted by digeneans (Platyhelminthes, Trematoda). These endobacteria can also infect vertebrate hosts of the helminths and cause serious diseases in animals and humans. Neorickettsia have been isolated from infected animals and maintained in ce...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2123-7
更新日期:2017-04-13 00:00:00
abstract:BACKGROUND:Toxoplasmosis is an important zoonosis, being a cause of congenital disease and abortion in animals and humans. DNA vaccination as a promising vaccine remains a challenge for an improved delivery system. METHODS:In this study, attenuated Salmonella typhimurium BRD509 was used to deliver a DNA vaccine encodi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-145
更新日期:2014-03-31 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis (VL) is a neglected disease that is spread to humans by the bites of infected female phlebotomine sand flies. Although this vector-borne disease has been eliminated in most parts of China, it still poses a significant public health burden in the Xinjiang Uygur Autonomous Region. Unders...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3778-z
更新日期:2019-11-08 00:00:00
abstract:BACKGROUND:Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneou...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2568-8
更新日期:2017-12-16 00:00:00
abstract:BACKGROUND:The relationship between the environment and infection of cattle with trematodes was studied at Arumeru District, Arusha Region, northern Tanzania. Randomly selected villages were grouped into three cattle management strata, (i) zero-grazing (ZZ) (ii) communal grazing without irrigation (ZC) and (iii) commun...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-107
更新日期:2014-03-20 00:00:00
abstract:BACKGROUND:Sheep scab is a highly contagious disease of sheep caused by the ectoparasitic mite Psoroptes ovis. The disease is endemic in the UK and has significant economic impact through its effects on performance and welfare. Diagnosis of sheep scab is achieved through observation of clinical signs e.g. itching, prur...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-7
更新日期:2012-01-10 00:00:00
abstract:BACKGROUND:West African Dwarf (WAD) goats serve an important role in the rural village economy of West Africa, especially among small-holder livestock owners. They have been shown to be trypanotolerant and to resist infections with Haemonchus contortus more effectively than any other known breed of goat. METHODS:In th...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-4-12
更新日期:2011-02-03 00:00:00
abstract:BACKGROUND:The Anopheles gambiae Giles complex is the most widely studied and the most important insect vector group. We explored the use of the palp ratio method as a field tool to identify A. melas and A. gambiae in Ghana. METHODS:Human landing catches were conducted to collect mosquitoes in the coastal area of West...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0913-3
更新日期:2015-05-28 00:00:00
abstract:BACKGROUND:To reveal the spatio-temporal distribution of malaria vectors in the national malaria surveillance sites from 2005 to 2010 and provide reference for the current National Malaria Elimination Programme (NMEP) in China. METHODS:A 6-year longitudinal surveillance on density of malaria vectors was carried out in...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0741-5
更新日期:2015-03-07 00:00:00
abstract:BACKGROUND:In 2012, the World Health Organization set goals for the elimination of onchocerciasis transmission by 2020 in selected African countries. Epidemiological data and mathematical modelling have indicated that elimination may not be achieved with annual ivermectin distribution in all endemic foci. Complementary...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2864-y
更新日期:2018-05-29 00:00:00
abstract:BACKGROUND:The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylse...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2198-1
更新日期:2017-05-30 00:00:00
abstract:BACKGROUND:Aedes albopictus is widely distributed across tropical and sub-tropical regions and is associated with the transmission of several arboviruses. Although this species is increasingly relevant to public health due its ability to successfully colonize both urban and rural habitats, favoring the dispersion of vi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-253
更新日期:2014-05-30 00:00:00
abstract:BACKGROUND:Ixodes ricinus is the most common tick species in Europe and the main vector for Borrelia burgdorferi (sensu lato) and tick-borne encephalitis virus (TBEV). It is involved also in the transmission of Borrelia miyamotoi, a relapsing fever spirochete that causes health disorders in humans. Little is known rega...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3969-7
更新日期:2020-02-27 00:00:00
abstract::Here we describe a case of paediatric visceral leishmaniasis recorded in an infant initially suspected for acute lymphoblastic leukaemia due to the clinical and haematological presentation. Eventually the patient was found positive for Leishmania infantum infection and successfully treated. This case emphasises how pi...
journal_title:Parasites & vectors
pub_type: 信件
doi:10.1186/1756-3305-6-123
更新日期:2013-04-29 00:00:00
abstract:BACKGROUND:The transformation of noninfective epimastigotes into infective metacyclic trypomastigotes (metacyclogenesis) is a fundamental step in the life cycle of Trypanosoma cruzi, comprising several morphological and biochemical changes. GP82 and GP90 are glycoproteins expressed at the surface of metacyclic trypomas...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-127
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:The prolonged feeding process of ixodid ticks, in combination with bacterial transmission, should lead to a robust inflammatory response at the blood-feeding site. Yet, factors present in tick saliva may down-regulate such responses, which may be beneficial to spirochete transmission. The primary goal of thi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1638-7
更新日期:2016-07-08 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Parasites & vectors
pub_type: 已发布勘误
doi:10.1186/s13071-020-04349-y
更新日期:2020-09-22 00:00:00
abstract:BACKGROUND:Nosema bombycis is a unicellular eukaryotic pathogen of the silkworm, Bombyx mori, and is an economic and occupational hazard in the silkworm industry. Because of its long incubation period and horizontal and vertical transmission, it is subject to quarantine measures in sericulture production. The microspor...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-021-04595-8
更新日期:2021-01-25 00:00:00
abstract:BACKGROUND:Glutathione S-transferases (GSTs) facilitate detoxification of drugs by catalysing the conjugation of the reduced glutathione (GSH) to electrophilic xenobiotic substrates and therefore have a function in multi-drug resistance. As a result, knowledge of GSTs can inform both drug resistance in, and novel inter...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0960-9
更新日期:2015-06-26 00:00:00
abstract:BACKGROUND:This study was carried out to develop a health education learning package (HELP) about soil-transmitted helminth (STH) infections, and to evaluate what impact such a package could have in terms of reducing the incidence and intensity of STH infections among Orang Asli schoolchildren in Pahang, Malaysia. MET...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-416
更新日期:2014-09-02 00:00:00
abstract:BACKGROUND:Recent studies have reported that soft tunic syndrome (STS) in the edible ascidian Halocynthia roretzi is caused by the kinetoplastid parasite Azumiobodo hoyamushi. In this study, we attempted to detect and quantify the pathogen in benthic animals. METHODS:Four species of ascidians, three species of echinod...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1179-5
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. FINDINGS:Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were se...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-178
更新日期:2013-06-17 00:00:00
abstract:BACKGROUND:There has been increasing effort in recent years to incorporate user needs in technology design and re-design. This project employed a bottom-up approach that engaged end users from the outset. Bottom-up approaches have the potential to bolster novel interventions and move them towards adaptive and evidence-...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0523-5
更新日期:2014-11-18 00:00:00
abstract:BACKGROUND:During infection with Leishmania donovani, parasite control is linked to the systemic Th1 immune response, but in infected organs (liver, spleen and bone marrow), the response differs according to the micro-environment. The pleiomorphic cytokine interleukin-33 (IL-33) exerts various roles during infection, e...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04190-3
更新日期:2020-06-22 00:00:00